LOCUS       NM_009266               2206 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus selenophosphate synthetase 2 (Sephs2), mRNA.
ACCESSION   NM_009266
VERSION     NM_009266.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Eagle,K., Jiang,Y., Shi,X., Li,M., Obholzer,N.P., Hu,T.,
            Perez,M.W., Koren,J.V., Kitano,A., Yi,J.S., Lin,C.Y. and Nakada,D.
  TITLE     An oncogenic enhancer encodes selective selenium dependency in AML
  JOURNAL   Cell Stem Cell 29 (3), 386-399 (2022)
   PUBMED   35108519
  REMARK    Erratum:[Cell Stem Cell. 2022 Apr 7;29(4):650. doi:
            10.1016/j.stem.2022.03.006.. PMID: 35395189]
REFERENCE   3  (bases 1 to 2206)
  AUTHORS   Gladyshev,V.N., Arner,E.S., Berry,M.J., Brigelius-Flohe,R.,
            Bruford,E.A., Burk,R.F., Carlson,B.A., Castellano,S., Chavatte,L.,
            Conrad,M., Copeland,P.R., Diamond,A.M., Driscoll,D.M., Ferreiro,A.,
            Flohe,L., Green,F.R., Guigo,R., Handy,D.E., Hatfield,D.L.,
            Hesketh,J., Hoffmann,P.R., Holmgren,A., Hondal,R.J., Howard,M.T.,
            Huang,K., Kim,H.Y., Kim,I.Y., Kohrle,J., Krol,A., Kryukov,G.V.,
            Lee,B.J., Lee,B.C., Lei,X.G., Liu,Q., Lescure,A., Lobanov,A.V.,
            Loscalzo,J., Maiorino,M., Mariotti,M., Sandeep Prabhu,K.,
            Rayman,M.P., Rozovsky,S., Salinas,G., Schmidt,E.E., Schomburg,L.,
            Schweizer,U., Simonovic,M., Sunde,R.A., Tsuji,P.A., Tweedie,S.,
            Ursini,F., Whanger,P.D. and Zhang,Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   4  (bases 1 to 2206)
  AUTHORS   Xu,X.M., Carlson,B.A., Irons,R., Mix,H., Zhong,N., Gladyshev,V.N.
            and Hatfield,D.L.
  TITLE     Selenophosphate synthetase 2 is essential for selenoprotein
            biosynthesis
  JOURNAL   Biochem J 404 (1), 115-120 (2007)
   PUBMED   17346238
  REMARK    GeneRIF: These in vivo studies indicate that SPS2 is essential for
            generating the selenium donor for selenocysteine biosynthesis in
            mammals.
REFERENCE   5  (bases 1 to 2206)
  AUTHORS   Kim,I.Y., Guimaraes,M.J., Zlotnik,A., Bazan,J.F. and Stadtman,T.C.
  TITLE     Fetal mouse selenophosphate synthetase 2 (SPS2): characterization
            of the cysteine mutant form overproduced in a baculovirus-insect
            cell system
  JOURNAL   Proc Natl Acad Sci U S A 94 (2), 418-421 (1997)
   PUBMED   9012797
REFERENCE   6  (bases 1 to 2206)
  AUTHORS   Guimaraes,M.J., Peterson,D., Vicari,A., Cocks,B.G., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A., Ferrick,D.A., Kastelein,R.A.,
            Bazan,J.F. and Zlotnik,A.
  TITLE     Identification of a novel selD homolog from eukaryotes, bacteria,
            and archaea: is there an autoregulatory mechanism in selenocysteine
            metabolism?
  JOURNAL   Proc Natl Acad Sci U S A 93 (26), 15086-15091 (1996)
   PUBMED   8986768
REFERENCE   7  (bases 1 to 2206)
  AUTHORS   Guimaraes,M.J., Bazan,J.F., Zlotnik,A., Wiles,M.V., Grimaldi,J.C.,
            Lee,F. and McClanahan,T.
  TITLE     A new approach to the study of haematopoietic development in the
            yolk sac and embryoid bodies
  JOURNAL   Development 121 (10), 3335-3346 (1995)
   PUBMED   7588067
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK168722.1, AK149516.1 and
            U43285.1.
            
            On Apr 6, 2007 this sequence version replaced NM_009266.2.
            
            Summary: This gene encodes an enzyme that catalyzes the production
            of monoselenophosphate (MSP) from selenide and ATP. MSP is the
            selenium donor required for synthesis of selenocysteine (Sec),
            which is co-translationally incorporated into selenoproteins at
            in-frame UGA codons that normally signal translation termination.
            The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop
            structure, the Sec insertion sequence (SECIS) element, which is
            necessary for the recognition of UGA as a Sec codon rather than as
            a stop signal. This protein is itself a selenoprotein containing a
            Sec residue at its active site, suggesting the existence of an
            autoregulatory mechanism. It is preferentially expressed in tissues
            implicated in the synthesis of selenoproteins and in sites of blood
            cell development. [provided by RefSeq, May 2017].
            
            ##Evidence-Data-START##
            Transcript is intronless :: U43285.1, BC016508.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 8986768
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1760              AK168722.1         2-1761
            1761-2177           AK149516.1         1758-2174
            2178-2206           U43285.1           2174-2202
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.43 cM"
     gene            1..2206
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /note="selenophosphate synthetase 2"
                     /db_xref="GeneID:20768"
                     /db_xref="MGI:MGI:108388"
     exon            1..2181
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1499
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /EC_number="2.7.9.3"
                     /note="UGA stop codon recoded as selenocysteine; selenium
                     donor protein 2; selenophosphate synthase 2; the SelD gene
                     product"
                     /codon_start=1
                     /transl_except=(pos:327..329,aa:Sec)
                     /product="selenide, water dikinase 2"
                     /protein_id="NP_033292.2"
                     /db_xref="CCDS:CCDS21863.1"
                     /db_xref="GeneID:20768"
                     /db_xref="MGI:MGI:108388"
                     /translation="MAEAAAAGASGETMAALVAAEGSLGPAGWSAGRSFSNYRPFEPQ
                     TLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSGLVGGQEETVQE
                     GGLSTRPGPGSAFPSLSIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACAN
                     VLSDLYAMGITECDNMLMLLSVSQSMSEKEREKVTPLMIKGFRDAAEEGGTAVTGGQT
                     VVNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEKWN
                     KIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAK
                     QQKNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSE
                     IKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGASAAAAAAPDNSNAASEP
                     SS"
     regulatory      2007..2082
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /note="hexamer: AATAAA"
     regulatory      2158..2163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /note="hexamer: AATAAA"
     polyA_site      2175
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
     polyA_site      2181
                     /gene="Sephs2"
                     /gene_synonym="Sps2; Ysg3"
                     /note="major polyA site"
ORIGIN      
        1 aggcagcgtg gctcgagtcc tccggtccgg ctcgcgtggt tgatcatctc ctggcgtaac
       61 cttggcccgc tgtggctggg agactcatct gcaggtatct gggccttctg gtccgcacgg
      121 cctcccgggc gagcggcgcg atggcggaag cggcggcggc gggcgccagc ggagaaacca
      181 tggcggcgct agtggccgca gaaggttcct tgggcccggc gggctggtct gccggccgga
      241 gtttctccaa ctaccggccg ttcgagcccc agacactggg cttcagcccg agctggcggc
      301 tgacgagctt ctccggcatg aagggctgag gctgcaaggt cccccaggag accctgctca
      361 aactcctgga gggactgacg cggcccgcgc tgcagccccc gcttacctcg ggtctggtcg
      421 ggggccagga agagacggtg caggaagggg gcctgtccac caggcccggc cccggctcag
      481 ccttcccctc gctgagcatt ggcatggact cctgcgtcat ccccctgagg cacggaggcc
      541 tgtcgctggt gcagaccacc gacttctttt accccttggt ggaagatccc tatatgatgg
      601 ggcgcatagc ttgtgccaat gtgctcagtg acctctatgc catgggtatc actgagtgtg
      661 acaacatgtt gatgttactc agtgtgagcc agagcatgag tgaaaaggaa cgagagaagg
      721 tgacaccgct catgatcaaa ggctttcgtg acgctgcgga ggagggaggc actgcagtga
      781 ccggtggaca gacagtggtc aacccgtgga ttatcatcgg tggcgttgcc acggtggtgt
      841 gtcagcaaaa tgaattcata atgcctgata gtgccgtggt aggagatgtg ctggtattaa
      901 ccaaaccttt aggaacccag gttgctgcca atgcccacca atggctggat aatcctgaga
      961 aatggaataa aatcaagatg gtggtttcca gagaggaagt agagttagcc tatcaggaag
     1021 ctatgttcaa tatggctact ctaaacagga ctgctgctgg cttgatgcac acttttaatg
     1081 cccacgcagc cacggatatc acaggctttg gcatattagg acactctcag aacctggcaa
     1141 aacagcaaaa aaatgaagtg tcctttgtca tacataatct gccaataatt gccaagatgg
     1201 ctgcgatcag caaagccagt gggcgctttg gcctcctcca aggaacgtca gctgaaacct
     1261 ctgggggatt actgatttgt ctgccaagag agcaggcggc ccgcttttgt tcggaaatca
     1321 aatcttccaa gtacggagag ggtcaccaag cttggatcgt tggcatcgtg gagaagggaa
     1381 accggacagc ccggatcatt gacaagcctc gcgttattga agttctacct cggggagcct
     1441 ctgctgctgc tgctgctgct cctgataatt ccaacgcagc ctctgagcct agttcttgaa
     1501 atggaatagc ggttgttggg aactcggagc cattctaccc gctcagggac tgctggccag
     1561 ggttgatttt aagacctttc caaaggctgc ttgcatggtt cctccaggcc catccaaagc
     1621 ttcctgtatg tgcatccagg cctgtgagta atggcgctgc ggatgtgtgt tcatctgttg
     1681 agagcatgag gagcaaaaac ccgtttccca aagcaagagg aggctatttc agttttaggg
     1741 attttttttt tttttttttt ttgcactgag ttgattcatt tctgcacagg gagtaaagat
     1801 tattaagatt acatatgaga aaagtaaacc tgcaacatga aaaattattt ggaccaatat
     1861 attgataaat ctaaattgtt aggagaactc ttactgattt attgtcaaat ttgttattaa
     1921 tttttttctg agaaactgcc tcttttcctg ttctggacaa gagttgagca gcttgtccga
     1981 caggaaagga agactagcca cctgacttgg tctctgataa tgatgtctct ccctctaact
     2041 cccagtaagg actgggagag gctgaacaaa cctcagagcc aggtgtcggt ggccattgaa
     2101 tcttacactg aaacttctgg agatttaatt aataaagaga atttcttaca gtaactaaat
     2161 aaaagggact ttgttggaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//
