LOCUS       NM_175400               5329 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus selenophosphate synthetase 1 (Sephs1), transcript
            variant 2, mRNA.
ACCESSION   NM_175400
VERSION     NM_175400.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Bang,J., Kang,D., Jung,J., Yoo,T.J., Shim,M.S., Gladyshev,V.N.,
            Tsuji,P.A., Hatfield,D.L., Kim,J.H. and Lee,B.J.
  TITLE     SEPHS1: Its evolution, function and roles in development and
            diseases
  JOURNAL   Arch Biochem Biophys 730, 109426 (2022)
   PUBMED   36202216
  REMARK    GeneRIF: SEPHS1: Its evolution, function and roles in development
            and diseases.
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Kang,D., Lee,J., Jung,J., Carlson,B.A., Chang,M.J., Chang,C.B.,
            Kang,S.B., Lee,B.C., Gladyshev,V.N., Hatfield,D.L., Lee,B.J. and
            Kim,J.H.
  TITLE     Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis
            by dysregulating redox homeostasis
  JOURNAL   Nat Commun 13 (1), 779 (2022)
   PUBMED   35140209
  REMARK    GeneRIF: Selenophosphate synthetase 1 deficiency exacerbates
            osteoarthritis by dysregulating redox homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5329)
  AUTHORS   Qiao,L., Dho,S.H., Kim,J.Y. and Kim,L.K.
  TITLE     SEPHS1 is dispensable for pluripotency maintenance but
            indispensable for cardiac differentiation in mouse embryonic stem
            cells
  JOURNAL   Biochem Biophys Res Commun 590, 125-131 (2022)
   PUBMED   34974300
  REMARK    GeneRIF: SEPHS1 is dispensable for pluripotency maintenance but
            indispensable for cardiac differentiation in mouse embryonic stem
            cells.
REFERENCE   4  (bases 1 to 5329)
  AUTHORS   Bang,J., Han,M., Yoo,T.J., Qiao,L., Jung,J., Na,J., Carlson,B.A.,
            Gladyshev,V.N., Hatfield,D.L., Kim,J.H., Kim,L.K. and Lee,B.J.
  TITLE     Identification of Signaling Pathways for Early Embryonic Lethality
            and Developmental Retardation in Sephs1-/- Mice
  JOURNAL   Int J Mol Sci 22 (21), 11647 (2021)
   PUBMED   34769078
  REMARK    GeneRIF: Identification of Signaling Pathways for Early Embryonic
            Lethality and Developmental Retardation in Sephs1(-/-) Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5329)
  AUTHORS   Jung,J., Kim,Y., Na,J., Qiao,L., Bang,J., Kwon,D., Yoo,T.J.,
            Kang,D., Kim,L.K., Carlson,B.A., Hatfield,D.L., Kim,J.H. and
            Lee,B.J.
  TITLE     Constitutive Oxidative Stress by SEPHS1 Deficiency Induces
            Endothelial Cell Dysfunction
  JOURNAL   Int J Mol Sci 22 (21), 11646 (2021)
   PUBMED   34769076
  REMARK    GeneRIF: Constitutive Oxidative Stress by SEPHS1 Deficiency Induces
            Endothelial Cell Dysfunction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5329)
  AUTHORS   Tobe,R., Carlson,B.A., Huh,J.H., Castro,N.P., Xu,X.M., Tsuji,P.A.,
            Lee,S.G., Bang,J., Na,J.W., Kong,Y.Y., Beaglehole,D., Southon,E.,
            Seifried,H., Tessarollo,L., Salomon,D.S., Schweizer,U.,
            Gladyshev,V.N., Hatfield,D.L. and Lee,B.J.
  TITLE     Selenophosphate synthetase 1 is an essential protein with roles in
            regulation of redox homoeostasis in mammals
  JOURNAL   Biochem J 473 (14), 2141-2154 (2016)
   PUBMED   27208177
  REMARK    GeneRIF: Data found that several malignant characteristics of
            SPS1-deficient F9 cells were reversed, suggesting that SPS1 played
            a role in supporting and/or sustaining cancer. Selenophosphate
            synthetase 1 is an essential protein with roles in regulation of
            redox homoeostasis in mammals.
REFERENCE   7  (bases 1 to 5329)
  AUTHORS   Saiki,R., Nagata,A., Kainou,T., Matsuda,H. and Kawamukai,M.
  TITLE     Characterization of solanesyl and decaprenyl diphosphate synthases
            in mice and humans
  JOURNAL   FEBS J 272 (21), 5606-5622 (2005)
   PUBMED   16262699
  REMARK    GeneRIF: murine and human solanesyl and decaprenyl diphosphate
            synthases are heterotetramers composed of newly characterized hDPS1
            (mSPS1) and hDLP1 (mDLP1)
REFERENCE   8  (bases 1 to 5329)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 5329)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC066037.1 and BC065165.1.
            
            On Sep 1, 2007 this sequence version replaced NM_175400.5.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066037.1, SRR1660817.31012.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               BC066037.1         1-198
            199-312             BC065165.1         159-272
            313-1056            BC066037.1         312-1055
            1057-1084           BC065165.1         1018-1045
            1085-5329           BC066037.1         1084-5328
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 3.02 cM"
     gene            1..5329
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="selenophosphate synthetase 1"
                     /db_xref="GeneID:109079"
                     /db_xref="MGI:MGI:1923580"
     exon            1..297
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            298..571
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..1557
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /EC_number="2.7.9.3"
                     /note="isoform a is encoded by transcript variant 2;
                     selenide, water dikinase 1; selenium donor protein 1;
                     selenophosphate synthase 1"
                     /codon_start=1
                     /product="selenide, water dikinase 1 isoform a"
                     /protein_id="NP_780609.4"
                     /db_xref="CCDS:CCDS15659.1"
                     /db_xref="GeneID:109079"
                     /db_xref="MGI:MGI:1923580"
                     /translation="MSTRESFNPETYELDKSFRLTRFTELKGTGCKVPQDVLQKLLES
                     LQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRI
                     ACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVIPLIIQGFKDAAEEAGTSVT
                     GGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIP
                     EKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQ
                     NLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAA
                     RFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS
                     "
     exon            572..675
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..783
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            784..938
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1029
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1129
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1342
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..5319
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3226..3231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="hexamer: AATAAA"
     polyA_site      3262
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="major polyA site"
     regulatory      5298..5303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="hexamer: AATAAA"
     polyA_site      5319
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="major polyA site"
ORIGIN      
        1 ggaggcgctc cggccaccgg cccgccgcct taaagggagg tggccgctct taaagggacc
       61 ctcacgcgcc cggccggcgg gagcgcggcg gcccggttcc cggagggccg cgcccagcct
      121 aggccgagct ccacgcggcg tccgagcgcc cggcggtgcc gccgccaccg ggagcagcat
      181 tttttttttt ttttttttta aattcaggcg acgcttaagg gagcccagcg cgcccggtgc
      241 attgtgggag cgccgcggcc cgttttcggg aggaggcgga gggcgcaaag cgagccggtg
      301 gatcacaagt agaaagccca gtggatctag agagagggag gggaggggct gagctgtgga
      361 gagtgcggcc ccagaaccat gtctactcga gagtccttta acccggagac ttatgaattg
      421 gacaagagct tccggctaac caggtttact gaactgaaag gcacaggctg caaagtgccc
      481 caagatgtcc tacagaaact gctggaatct ttacaagaga accacttcca agaagatgag
      541 cagtttctgg gagctgttat gccacgactt ggcattggga tggatacttg cgtcattcct
      601 ttgaggcatg gtggtctttc cttggttcag accacagatt acatttatcc tatcgtcgac
      661 gacccgtata tgatgggcag gatagcatgt gccaatgtcc tcagtgacct ttatgcaatg
      721 ggtgtcacgg agtgtgacaa tatgctgatg ctccttggag tcagtaataa aatgactgac
      781 cgggagaggg ataaagtgat accgctaatt atacagggtt ttaaagatgc ggcagaggaa
      841 gcgggaacct ctgtaacagg cggccaaacg gtattaaacc cctggattgt tctgggagga
      901 gtcgccacaa cggtctgcca gcccaatgaa tttatcatgc cagataatgc agtacctggg
      961 gatgtgctgg tattgacaaa gcccctgggg acacaggttg cagttgctgt gcaccagtgg
     1021 ctggatattc ccgaaaaatg gaataaaatt aagctagtgg tcacccaaga agacgtagag
     1081 ttggcatacc aagaggcaat gatgaacatg gcccggctca acaggacagc tgcaggcctc
     1141 atgcacacgt tcaatgctca tgcagccact gacatcaccg gcttcgggat tctgggccac
     1201 gcgcagaacc tggccaagca acagaggaac gaagtgtcct tcgtgattca caaccttcct
     1261 gtgctggcga agatggccgc tgtgagcaaa gcctgcggaa acatgttcgg cctaatgcat
     1321 gggacctgcc cagagacgtc aggaggcctg ctaatctgtc taccccgtga gcaagcagct
     1381 cggttctgtg cagagatcaa gtcccccaaa tacggcgaag ggcaccaagc atggattatt
     1441 gggatagtgg agaagggcaa ccgcacagcc aggatcatcg acaagcctcg gattattgaa
     1501 gtcgcacctc aagtagccac acaaaacgtg aaccccacac ctggtgccac ctcctaatcc
     1561 agacagagct atttgatttt gtttttaaat agatctattt cctttatcat cacttcaatt
     1621 aaagactcta agaacaacaa aatctcattg tgtctacaca tctggtgacc ctaggtcggt
     1681 ttatgagtgg atgcaattaa taaaatgaaa tccatggccg ccttttcctg ttacattaac
     1741 tgaagatgca cccgacctcc aggcagcttc tgagttgaga gtgatcctgt tatccaatcc
     1801 tgttgattca ttttgaatct ttagctgtgt gtctcttgcc gcataggcgc tctctaaagg
     1861 tgcttttctg ccactcgggc attgctgaga ggagtgccac tagccttctg tgtcttctca
     1921 tttctgcctc tcatctcagt ctgatctgtt gtcaagtgtc tcagttgaca ttctagaaag
     1981 acagaatggt aagtgacatg atggtctccc cgtagtcaga gggttgcatg gctccttcga
     2041 gtgtttgatt tgtaaagttg agtcgaataa ctcgagggac ttcttatcca gtagctctct
     2101 cagttcagta tggcaggtgc tttgcttggc atggaatgca cttggaatac aaaaaaggaa
     2161 tgttctttta gaacaaacaa agctttcctg agacttgcag taataccatt aatggttttt
     2221 actaataggg aaaaggatac tttttgcagt gttctttaga tgttctatag tgcgacgagg
     2281 aactgccttc cgatgcgggg gttcatgtat aatactcatt tataattcaa tatataattt
     2341 acttcgcaaa taatttttaa atataatcaa tactaaggac tgttgtgtgg atggtagtgt
     2401 tgaatacatt ttatattttg tattgtgatt ccaggcctct tgtttaaaat cagcagctct
     2461 ttagcccaag tcttagcaca tcttgtcttg gcacatcttg tctttgtgca cgaattctgt
     2521 gatttgtgtt aaaaacctgc attgtcaaca ttgcagctcc aacataaaag ttgttattca
     2581 aataaatatt taattttttt aattgctctt gtataatcag atgccctttt tagtattatt
     2641 ttagaagcat ttgggaggat tttgcctaaa atacaattta ttgggggaga ctagatttta
     2701 gttttataaa cttttaagtc tttcatggga cctatatttt cttgaattaa attttgtagt
     2761 tctagaacaa ataggcaatc tacaaaggtg ttatctgtgt ttcttaaaac caggcttcct
     2821 tatcttcctt atctgttaag cagctggggg attcccgtct ttaagcacaa gggcactggg
     2881 tcctcaaacg cctgacttca aaagagaaaa agcaccaagt cttcaggttt cacagggctt
     2941 cctactaaca ggacaggccc caaagctgag ctctgcaaac tcacccaacc tcactcctca
     3001 acacggaaca ccagaaaacc gccactggac cagcggaact gcggaaggaa gaggctgcca
     3061 cgtgcttgtg tccccagaat ctcctgtaaa tgcacctctt actggaactg aagccaccag
     3121 agtctggccc cagcagagga agctgtcaca gaaggacact aattggacac tatataggaa
     3181 tctataagat gttggtcctt gaaagtatgc accagtggtc tgcaaaataa aacatgttgg
     3241 aaccctctga gtgcagaaac tatactgttg gacttgcttc tattttgttt tgttttgttt
     3301 tttaaatagt aggtgaaatt ggagctaact ctgtatgttc tgttccccaa agccactact
     3361 tttcttggag gcaagcctgt tgggaggaag ttagatggtt aatgttctga gcaaattaaa
     3421 gattcatata aggagaaaat gccaggggct taagagatcc atggattcgt gatctaggaa
     3481 aagccttgtc ttgactagtg ttttcagggt gctcggtaac cgatgcatta gaatcccctg
     3541 ggtgtttgtg gaggaggcct ttctgcactt tcctgagacc cagagtcagt tctggctgtg
     3601 gaaccaggta gtccatctct ccaatgtacc ctgtagggct ctgcataaca acccttgaga
     3661 accactaatc ttaaaaggaa tgttgggaga ggtaaaaagt ggggctgccg ggggtcactc
     3721 tagtcttagc cattcctctt tattttcagt ggctctatgt gtgtgtgtaa agttccttga
     3781 ctcaagtgac tgtcattgtt taataagttt ggcaaagtct acatgggact cttaatgtgt
     3841 ccagcagtca cgccttgccg acttctggta ccagtctaaa attccaccct ggagaaattc
     3901 caccctagtg tcagatcctc tcataattaa agtttagcag gttatttcca caggctgggt
     3961 tgaagcaaca acattcttgt tctttttttg gctcagtatt ttgaaatatc taagtcttcc
     4021 aaattctgca tgagttgtaa tgaataagtg gaagtttctg attgttctga tgcatgagcg
     4081 agcggccctt gcatctgagt tcagcattgc ctttaaattg ttctgttttg agctgtgtaa
     4141 gatggctcag cgggtacagc ggcttgtctc ataaggctga gctccatctg tctgcagaac
     4201 ccatgtgcaa agaacagtga cctgcacgta catcgtgtgc tatgtactca tgcacctagc
     4261 aataaataca ttaatatttc attgtcagag ttctagatct tcattttatt ctgtagaaac
     4321 agaaataata gctatactag tttattttta cagatgctta gcacaggcag gtgtttgaga
     4381 gatctgatgg tgcgcatgca cacacacaaa ccctgcttga acgctcgttt tcatatctga
     4441 ccaggtgctc acacaacata ctctgcttcc aacactcgag tatcccattc tcattaagaa
     4501 tccacataag gattgcagtc atgaaacatc agaggccaca gtgaaaatcc aaagtccttt
     4561 tgaatgagga gtgggtggat tagaccaggc ctgagtcaac taacaggcag catctctagg
     4621 catccagaca tggtcaagaa cccctggaag ccaaacttgc tgtacaaggg tgcgcttgtg
     4681 gtttgtctgg tgagtgagta ttgggtgcat aggtcttaac attctctgca gtttcctatt
     4741 atgatagacc tcacatatca acactgctgg cattgatggt ccttgctagg tcgtgctgcc
     4801 ttttaagagg ttaatgccca ggacaacagc atgtaacaaa gtccagcgac tgtggagctg
     4861 ggtctagtga cacagggctg taatcccaca tactcaggag gccgaagcaa gaggacctcg
     4921 ttcaaagtct gcctgggcaa cctaataagg ccctgctttg ctctcctcca gcacaaagga
     4981 tcctaaaaag ttcgaaagta tagcttagtg gtagagtatt tgcctagcat tcacaaggcc
     5041 ctgggtttag tcctcagtag cagcaaaaac caaacttgat gaaaacaaga ttaaacgtca
     5101 ctagtttact gtaattacag tgtgattgtg ggacaaggct gatgcagcca ccattgtatt
     5161 tcaggaataa aaacatgtac gtggaaatat atagaatgta atgtttatca cccaagcata
     5221 ttttgcacac ttttaaagca aaattatata aaatttaatg tctaggatat attcctgggt
     5281 ttgggtccac tttgagaaat aaaactataa tagtttaaaa aaaaaaaaa
//
