LOCUS       NM_001362709            5211 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus selenophosphate synthetase 1 (Sephs1), transcript
            variant 4, mRNA.
ACCESSION   NM_001362709
VERSION     NM_001362709.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Bang,J., Kang,D., Jung,J., Yoo,T.J., Shim,M.S., Gladyshev,V.N.,
            Tsuji,P.A., Hatfield,D.L., Kim,J.H. and Lee,B.J.
  TITLE     SEPHS1: Its evolution, function and roles in development and
            diseases
  JOURNAL   Arch Biochem Biophys 730, 109426 (2022)
   PUBMED   36202216
  REMARK    GeneRIF: SEPHS1: Its evolution, function and roles in development
            and diseases.
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Kang,D., Lee,J., Jung,J., Carlson,B.A., Chang,M.J., Chang,C.B.,
            Kang,S.B., Lee,B.C., Gladyshev,V.N., Hatfield,D.L., Lee,B.J. and
            Kim,J.H.
  TITLE     Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis
            by dysregulating redox homeostasis
  JOURNAL   Nat Commun 13 (1), 779 (2022)
   PUBMED   35140209
  REMARK    GeneRIF: Selenophosphate synthetase 1 deficiency exacerbates
            osteoarthritis by dysregulating redox homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5211)
  AUTHORS   Qiao,L., Dho,S.H., Kim,J.Y. and Kim,L.K.
  TITLE     SEPHS1 is dispensable for pluripotency maintenance but
            indispensable for cardiac differentiation in mouse embryonic stem
            cells
  JOURNAL   Biochem Biophys Res Commun 590, 125-131 (2022)
   PUBMED   34974300
  REMARK    GeneRIF: SEPHS1 is dispensable for pluripotency maintenance but
            indispensable for cardiac differentiation in mouse embryonic stem
            cells.
REFERENCE   4  (bases 1 to 5211)
  AUTHORS   Bang,J., Han,M., Yoo,T.J., Qiao,L., Jung,J., Na,J., Carlson,B.A.,
            Gladyshev,V.N., Hatfield,D.L., Kim,J.H., Kim,L.K. and Lee,B.J.
  TITLE     Identification of Signaling Pathways for Early Embryonic Lethality
            and Developmental Retardation in Sephs1-/- Mice
  JOURNAL   Int J Mol Sci 22 (21), 11647 (2021)
   PUBMED   34769078
  REMARK    GeneRIF: Identification of Signaling Pathways for Early Embryonic
            Lethality and Developmental Retardation in Sephs1(-/-) Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5211)
  AUTHORS   Jung,J., Kim,Y., Na,J., Qiao,L., Bang,J., Kwon,D., Yoo,T.J.,
            Kang,D., Kim,L.K., Carlson,B.A., Hatfield,D.L., Kim,J.H. and
            Lee,B.J.
  TITLE     Constitutive Oxidative Stress by SEPHS1 Deficiency Induces
            Endothelial Cell Dysfunction
  JOURNAL   Int J Mol Sci 22 (21), 11646 (2021)
   PUBMED   34769076
  REMARK    GeneRIF: Constitutive Oxidative Stress by SEPHS1 Deficiency Induces
            Endothelial Cell Dysfunction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5211)
  AUTHORS   Tobe,R., Carlson,B.A., Huh,J.H., Castro,N.P., Xu,X.M., Tsuji,P.A.,
            Lee,S.G., Bang,J., Na,J.W., Kong,Y.Y., Beaglehole,D., Southon,E.,
            Seifried,H., Tessarollo,L., Salomon,D.S., Schweizer,U.,
            Gladyshev,V.N., Hatfield,D.L. and Lee,B.J.
  TITLE     Selenophosphate synthetase 1 is an essential protein with roles in
            regulation of redox homoeostasis in mammals
  JOURNAL   Biochem J 473 (14), 2141-2154 (2016)
   PUBMED   27208177
  REMARK    GeneRIF: Data found that several malignant characteristics of
            SPS1-deficient F9 cells were reversed, suggesting that SPS1 played
            a role in supporting and/or sustaining cancer. Selenophosphate
            synthetase 1 is an essential protein with roles in regulation of
            redox homoeostasis in mammals.
REFERENCE   7  (bases 1 to 5211)
  AUTHORS   Saiki,R., Nagata,A., Kainou,T., Matsuda,H. and Kawamukai,M.
  TITLE     Characterization of solanesyl and decaprenyl diphosphate synthases
            in mice and humans
  JOURNAL   FEBS J 272 (21), 5606-5622 (2005)
   PUBMED   16262699
  REMARK    GeneRIF: murine and human solanesyl and decaprenyl diphosphate
            synthases are heterotetramers composed of newly characterized hDPS1
            (mSPS1) and hDLP1 (mDLP1)
REFERENCE   8  (bases 1 to 5211)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 5211)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL807778.10.
            
            Transcript Variant: This variant (4) differs in the 5' UTR and
            lacks an alternate in-frame exon compared to variant 1. The
            resulting isoform (b) has the same N- and C-termini but is shorter
            compared to isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.62970.1,
                                           SRR1660811.254069.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-297               AL807778.10        158485-158781
            298-571             AL807778.10        161253-161526
            572-675             AL807778.10        166104-166207
            676-830             AL807778.10        170106-170260
            831-921             AL807778.10        173610-173700
            922-1021            AL807778.10        176385-176484
            1022-1234           AL807778.10        182393-182605
            1235-5211           AL807778.10        183502-187478
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 3.02 cM"
     gene            1..5211
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="selenophosphate synthetase 1"
                     /db_xref="GeneID:109079"
                     /db_xref="MGI:MGI:1923580"
     exon            1..297
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            298..571
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..1449
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /EC_number="2.7.9.3"
                     /note="isoform b is encoded by transcript variant 4;
                     selenide, water dikinase 1; selenium donor protein 1;
                     selenophosphate synthase 1"
                     /codon_start=1
                     /product="selenide, water dikinase 1 isoform b"
                     /protein_id="NP_001349638.1"
                     /db_xref="GeneID:109079"
                     /db_xref="MGI:MGI:1923580"
                     /translation="MSTRESFNPETYELDKSFRLTRFTELKGTGCKVPQDVLQKLLES
                     LQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMERD
                     KVIPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDV
                     LVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGL
                     MHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGL
                     MHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKP
                     RIIEVAPQVATQNVNPTPGATS"
     exon            572..675
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..830
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..921
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1021
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1234
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..5211
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3118..3123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="hexamer: AATAAA"
     polyA_site      3154
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="major polyA site"
     regulatory      5190..5195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="hexamer: AATAAA"
     polyA_site      5211
                     /gene="Sephs1"
                     /gene_synonym="1110046B24Rik; SELD; SPS; SPS1"
                     /note="major polyA site"
ORIGIN      
        1 ggaggcgctc cggccaccgg cccgccgcct taaagggagg tggccgctct taaagggacc
       61 ctcacgcgcc cggccggcgg gagcgcggcg gcccggttcc cggagggccg cgcccagcct
      121 aggccgagct ccacgcggcg tccgagcgcc cggcggtgcc gccgccaccg ggagcagcat
      181 tttttttttt ttttttttta aattcaggcg acgcttaagg gagcccagcg cgcccggtgc
      241 attgtgggag cgccgcggcc cgttttcggg aggaggcgga gggcgcaaag cgagccggtg
      301 gatcacaagt agaaagccca gtggatctag agagagggag gggaggggct gagctgtgga
      361 gagtgcggcc ccagaaccat gtctactcga gagtccttta acccggagac ttatgaattg
      421 gacaagagct tccggctaac caggtttact gaactgaaag gcacaggctg caaagtgccc
      481 caagatgtcc tacagaaact gctggaatct ttacaagaga accacttcca agaagatgag
      541 cagtttctgg gagctgttat gccacgactt ggcattggga tggatacttg cgtcattcct
      601 ttgaggcatg gtggtctttc cttggttcag accacagatt acatttatcc tatcgtcgac
      661 gacccgtata tgatggagag ggataaagtg ataccgctaa ttatacaggg ttttaaagat
      721 gcggcagagg aagcgggaac ctctgtaaca ggcggccaaa cggtattaaa cccctggatt
      781 gttctgggag gagtcgccac aacggtctgc cagcccaatg aatttatcat gccagataat
      841 gcagtacctg gggatgtgct ggtattgaca aagcccctgg ggacacaggt tgcagttgct
      901 gtgcaccagt ggctggatat tcccgaaaaa tggaataaaa ttaagctagt ggtcacccaa
      961 gaagacgtag agttggcata ccaagaggca atgatgaaca tggcccggct caacaggaca
     1021 gctgcaggcc tcatgcacac gttcaatgct catgcagcca ctgacatcac cggcttcggg
     1081 attctgggcc acgcgcagaa cctggccaag caacagagga acgaagtgtc cttcgtgatt
     1141 cacaaccttc ctgtgctggc gaagatggcc gctgtgagca aagcctgcgg aaacatgttc
     1201 ggcctaatgc atgggacctg cccagagacg tcaggaggcc tgctaatctg tctaccccgt
     1261 gagcaagcag ctcggttctg tgcagagatc aagtccccca aatacggcga agggcaccaa
     1321 gcatggatta ttgggatagt ggagaagggc aaccgcacag ccaggatcat cgacaagcct
     1381 cggattattg aagtcgcacc tcaagtagcc acacaaaacg tgaaccccac acctggtgcc
     1441 acctcctaat ccagacagag ctatttgatt ttgtttttaa atagatctat ttcctttatc
     1501 atcacttcaa ttaaagactc taagaacaac aaaatctcat tgtgtctaca catctggtga
     1561 ccctaggtcg gtttatgagt ggatgcaatt aataaaatga aatccatggc cgccttttcc
     1621 tgttacatta actgaagatg cacccgacct ccaggcagct tctgagttga gagtgatcct
     1681 gttatccaat cctgttgatt cattttgaat ctttagctgt gtgtctcttg ccgcataggc
     1741 gctctctaaa ggtgcttttc tgccactcgg gcattgctga gaggagtgcc actagccttc
     1801 tgtgtcttct catttctgcc tctcatctca gtctgatctg ttgtcaagtg tctcagttga
     1861 cattctagaa agacagaatg gtaagtgaca tgatggtctc cccgtagtca gagggttgca
     1921 tggctccttc gagtgtttga tttgtaaagt tgagtcgaat aactcgaggg acttcttatc
     1981 cagtagctct ctcagttcag tatggcaggt gctttgcttg gcatggaatg cacttggaat
     2041 acaaaaaagg aatgttcttt tagaacaaac aaagctttcc tgagacttgc agtaatacca
     2101 ttaatggttt ttactaatag ggaaaaggat actttttgca gtgttcttta gatgttctat
     2161 agtgcgacga ggaactgcct tccgatgcgg gggttcatgt ataatactca tttataattc
     2221 aatatataat ttacttcgca aataattttt aaatataatc aatactaagg actgttgtgt
     2281 ggatggtagt gttgaataca ttttatattt tgtattgtga ttccaggcct cttgtttaaa
     2341 atcagcagct ctttagccca agtcttagca catcttgtct tggcacatct tgtctttgtg
     2401 cacgaattct gtgatttgtg ttaaaaacct gcattgtcaa cattgcagct ccaacataaa
     2461 agttgttatt caaataaata tttaattttt ttaattgctc ttgtataatc agatgccctt
     2521 tttagtatta ttttagaagc atttgggagg attttgccta aaatacaatt tattggggga
     2581 gactagattt tagttttata aacttttaag tctttcatgg gacctatatt ttcttgaatt
     2641 aaattttgta gttctagaac aaataggcaa tctacaaagg tgttatctgt gtttcttaaa
     2701 accaggcttc cttatcttcc ttatctgtta agcagctggg ggattcccgt ctttaagcac
     2761 aagggcactg ggtcctcaaa cgcctgactt caaaagagaa aaagcaccaa gtcttcaggt
     2821 ttcacagggc ttcctactaa caggacaggc cccaaagctg agctctgcaa actcacccaa
     2881 cctcactcct caacacggaa caccagaaaa ccgccactgg accagcggaa ctgcggaagg
     2941 aagaggctgc cacgtgcttg tgtccccaga atctcctgta aatgcacctc ttactggaac
     3001 tgaagccacc agagtctggc cccagcagag gaagctgtca cagaaggaca ctaattggac
     3061 actatatagg aatctataag atgttggtcc ttgaaagtat gcaccagtgg tctgcaaaat
     3121 aaaacatgtt ggaaccctct gagtgcagaa actatactgt tggacttgct tctattttgt
     3181 tttgttttgt tttttaaata gtaggtgaaa ttggagctaa ctctgtatgt tctgttcccc
     3241 aaagccacta cttttcttgg aggcaagcct gttgggagga agttagatgg ttaatgttct
     3301 gagcaaatta aagattcata taaggagaaa atgccagggg cttaagagat ccatggattc
     3361 gtgatctagg aaaagccttg tcttgactag tgttttcagg gtgctcggta accgatgcat
     3421 tagaatcccc tgggtgtttg tggaggaggc ctttctgcac tttcctgaga cccagagtca
     3481 gttctggctg tggaaccagg tagtccatct ctccaatgta ccctgtaggg ctctgcataa
     3541 caacccttga gaaccactaa tcttaaaagg aatgttggga gaggtaaaaa gtggggctgc
     3601 cgggggtcac tctagtctta gccattcctc tttattttca gtggctctat gtgtgtgtgt
     3661 aaagttcctt gactcaagtg actgtcattg tttaataagt ttggcaaagt ctacatggga
     3721 ctcttaatgt gtccagcagt cacgccttgc cgacttctgg taccagtcta aaattccacc
     3781 ctggagaaat tccaccctag tgtcagatcc tctcataatt aaagtttagc aggttatttc
     3841 cacaggctgg gttgaagcaa caacattctt gttctttttt tggctcagta ttttgaaata
     3901 tctaagtctt ccaaattctg catgagttgt aatgaataag tggaagtttc tgattgttct
     3961 gatgcatgag cgagcggccc ttgcatctga gttcagcatt gcctttaaat tgttctgttt
     4021 tgagctgtgt aagatggctc agcgggtaca gcggcttgtc tcataaggct gagctccatc
     4081 tgtctgcaga acccatgtgc aaagaacagt gacctgcacg tacatcgtgt gctatgtact
     4141 catgcaccta gcaataaata cattaatatt tcattgtcag agttctagat cttcatttta
     4201 ttctgtagaa acagaaataa tagctatact agtttatttt tacagatgct tagcacaggc
     4261 aggtgtttga gagatctgat ggtgcgcatg cacacacaca aaccctgctt gaacgctcgt
     4321 tttcatatct gaccaggtgc tcacacaaca tactctgctt ccaacactcg agtatcccat
     4381 tctcattaag aatccacata aggattgcag tcatgaaaca tcagaggcca cagtgaaaat
     4441 ccaaagtcct tttgaatgag gagtgggtgg attagaccag gcctgagtca actaacaggc
     4501 agcatctcta ggcatccaga catggtcaag aacccctgga agccaaactt gctgtacaag
     4561 ggtgcgcttg tggtttgtct ggtgagtgag tattgggtgc ataggtctta acattctctg
     4621 cagtttccta ttatgataga cctcacatat caacactgct ggcattgatg gtccttgcta
     4681 ggtcgtgctg ccttttaaga ggttaatgcc caggacaaca gcatgtaaca aagtccagcg
     4741 actgtggagc tgggtctagt gacacagggc tgtaatccca catactcagg aggccgaagc
     4801 aagaggacct cgttcaaagt ctgcctgggc aacctaataa ggccctgctt tgctctcctc
     4861 cagcacaaag gatcctaaaa agttcgaaag tatagcttag tggtagagta tttgcctagc
     4921 attcacaagg ccctgggttt agtcctcagt agcagcaaaa accaaacttg atgaaaacaa
     4981 gattaaacgt cactagttta ctgtaattac agtgtgattg tgggacaagg ctgatgcagc
     5041 caccattgta tttcaggaat aaaaacatgt acgtggaaat atatagaatg taatgtttat
     5101 cacccaagca tattttgcac acttttaaag caaaattata taaaatttaa tgtctaggat
     5161 atattcctgg gtttgggtcc actttgagaa ataaaactat aatagtttaa a
//
