LOCUS       NM_019979               1018 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus selenoprotein K (Selenok), mRNA.
ACCESSION   NM_019979
VERSION     NM_019979.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ouyang,P., Cai,Z., Peng,J., Lin,S., Chen,X., Chen,C., Feng,Z.,
            Wang,L., Song,G. and Zhang,Z.
  TITLE     SELENOK-dependent CD36 palmitoylation regulates microglial
            functions and Abeta phagocytosis
  JOURNAL   Redox Biol 70, 103064 (2024)
   PUBMED   38320455
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1018)
  AUTHORS   Lv,L., Chai,L., Wang,J., Wang,M., Qin,D., Song,H., Fu,Y., Zhao,C.,
            Jia,J., Zhao,W. and Jia,M.
  TITLE     Selenoprotein K enhances STING oligomerization to facilitate
            antiviral response
  JOURNAL   PLoS Pathog 19 (4), e1011314 (2023)
   PUBMED   37023217
  REMARK    GeneRIF: Selenoprotein K enhances STING oligomerization to
            facilitate antiviral response.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1018)
  AUTHORS   Wang,S., Zhao,X., Liu,Q., Wang,Y., Li,S. and Xu,S.
  TITLE     Selenoprotein K protects skeletal muscle from damage and is
            required for satellite cells-mediated myogenic differentiation
  JOURNAL   Redox Biol 50, 102255 (2022)
   PUBMED   35144051
  REMARK    GeneRIF: Selenoprotein K protects skeletal muscle from damage and
            is required for satellite cells-mediated myogenic differentiation.
REFERENCE   5  (bases 1 to 1018)
  AUTHORS   Jia,S.Z., Xu,X.W., Zhang,Z.H., Chen,C., Chen,Y.B., Huang,S.L.,
            Liu,Q., Hoffmann,P.R. and Song,G.L.
  TITLE     Selenoprotein K deficiency-induced apoptosis: A role for calpain
            and the ERS pathway
  JOURNAL   Redox Biol 47, 102154 (2021)
   PUBMED   34601426
  REMARK    GeneRIF: Selenoprotein K deficiency-induced apoptosis: A role for
            calpain and the ERS pathway.
REFERENCE   6  (bases 1 to 1018)
  AUTHORS   Huang,Z., Hoffmann,F.W., Fay,J.D., Hashimoto,A.C., Chapagain,M.L.,
            Kaufusi,P.H. and Hoffmann,P.R.
  TITLE     Stimulation of unprimed macrophages with immune complexes triggers
            a low output of nitric oxide by calcium-dependent neuronal
            nitric-oxide synthase
  JOURNAL   J Biol Chem 287 (7), 4492-4502 (2012)
   PUBMED   22205701
REFERENCE   7  (bases 1 to 1018)
  AUTHORS   Shchedrina,V.A., Everley,R.A., Zhang,Y., Gygi,S.P., Hatfield,D.L.
            and Gladyshev,V.N.
  TITLE     Selenoprotein K binds multiprotein complexes and is involved in the
            regulation of endoplasmic reticulum homeostasis
  JOURNAL   J Biol Chem 286 (50), 42937-42948 (2011)
   PUBMED   22016385
REFERENCE   8  (bases 1 to 1018)
  AUTHORS   Huang,Z., Hoffmann,F.W., Norton,R.L., Hashimoto,A.C. and
            Hoffmann,P.R.
  TITLE     Selenoprotein K is a novel target of m-calpain, and cleavage is
            regulated by Toll-like receptor-induced calpastatin in macrophages
  JOURNAL   J Biol Chem 286 (40), 34830-34838 (2011)
   PUBMED   21849499
  REMARK    GeneRIF: Selenoprotein K is a novel target of m-calpain, and
            cleavage is regulated by Toll-like receptor 2,3,4 and 9-induced
            calpastatin in macrophages
REFERENCE   9  (bases 1 to 1018)
  AUTHORS   Verma,S., Hoffmann,F.W., Kumar,M., Huang,Z., Roe,K., Nguyen-Wu,E.,
            Hashimoto,A.S. and Hoffmann,P.R.
  TITLE     Selenoprotein K knockout mice exhibit deficient calcium flux in
            immune cells and impaired immune responses
  JOURNAL   J Immunol 186 (4), 2127-2137 (2011)
   PUBMED   21220695
  REMARK    GeneRIF: Sel K-deficient T cells exhibit decreased chemokine
            receptor-mediated calcium flux.
REFERENCE   10 (bases 1 to 1018)
  AUTHORS   Kryukov,G.V., Castellano,S., Novoselov,S.V., Lobanov,A.V.,
            Zehtab,O., Guigo,R. and Gladyshev,V.N.
  TITLE     Characterization of mammalian selenoproteomes
  JOURNAL   Science 300 (5624), 1439-1443 (2003)
   PUBMED   12775843
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY095166.1, AK003206.1 and
            AK003280.1.
            
            On Aug 2, 2006 this sequence version replaced NM_019979.1.
            
            Summary: The protein encoded by this gene belongs to the
            selenoprotein K family. It is a transmembrane protein that is
            localized in the endoplasmic reticulum (ER), and is involved in
            ER-associated degradation (ERAD) of misfolded, glycosylated
            proteins. It also has a role in the protection of cells from ER
            stress-induced apoptosis. Knockout studies in mice show the
            importance of this gene in promoting Ca(2+) flux in immune cells
            and mounting effective immune response. This protein is a
            selenoprotein, containing the rare amino acid selenocysteine (Sec).
            Sec is encoded by the UGA codon, which normally signals translation
            termination. The 3' UTRs of selenoprotein mRNAs contain a conserved
            stem-loop structure, designated the Sec insertion sequence (SECIS)
            element, that is necessary for the recognition of UGA as a Sec
            codon, rather than as a stop signal. Multiple pseudogenes of this
            locus have been identified. [provided by RefSeq, Sep 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF170920.1, SRR1660813.185414.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 12775843
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                BY095166.1         1-73
            74-955              AK003206.1         2-883
            956-1018            AK003280.1         550-612
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 18.36 cM"
     gene            1..1018
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="selenoprotein K"
                     /db_xref="GeneID:80795"
                     /db_xref="MGI:MGI:1931466"
     exon            1..157
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..423
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="UGA stop codon recoded as selenocysteine; heat
                     shock protein, 30 kDa"
                     /codon_start=1
                     /transl_except=(pos:412..414,aa:Sec)
                     /product="selenoprotein K"
                     /protein_id="NP_064363.2"
                     /db_xref="CCDS:CCDS36844.1"
                     /db_xref="GeneID:80795"
                     /db_xref="MGI:MGI:1931466"
                     /translation="MVYISNGQVLDSRNQSPWRVSFLTDFFWGIAEFVVFFFKTLLQQ
                     DVKKRRGYGSSSDSRYDDGRGPPGNPPRRMGRISHLRGPSPPPMAGGUGR"
     exon            158..248
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /inference="alignment:Splign:2.1.0"
     exon            249..332
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /inference="alignment:Splign:2.1.0"
     exon            333..419
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /inference="alignment:Splign:2.1.0"
     exon            420..1018
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      526..627
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      758..763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="hexamer: AATAAA"
     polyA_site      784
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="major polyA site"
     regulatory      934..939
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
                     /note="hexamer: AATAAA"
     polyA_site      961
                     /gene="Selenok"
                     /gene_synonym="1110001C03Rik; Hsp30; HSPC030; Selk"
ORIGIN      
        1 atgggctcga cccacctcta gcctgacttg ggtctgtcag tggcctgtga cgtacagggc
       61 ggggcgggag gctaggtacg gcgacaacag gggcgtccgg tcttctctgt cgctaggaag
      121 caggcaaccg gagggaagat ggtttacatc tcgaatggtc aggtgttgga cagcaggaat
      181 cagtccccat ggagagtgtc tttcctaaca gacttcttct ggggaatagc agaattcgtg
      241 gttttctttt tcaaaactct gcttcagcaa gatgtgaaga aaagaagagg ctacgggagc
      301 tcctctgatt ccagatacga cgatggaaga gggccaccag gaaaccctcc acgaagaatg
      361 ggtaggatca gtcaccttcg tggccccagc cctcctccaa tggctggtgg atgaggaagg
      421 taaatgtctg ctctgagaag cagacaacta gacatacaca cgtgtagaag gaagccatca
      481 agcggtgaag agcggctcgt gcaggagaag atggtgtatg tccaaacaag gattgctctg
      541 tgtccccaca gatgaatgag gtcatgctgg gaattccctc tgcaggatct agcctgactg
      601 atacgcagtt ctataaatgt acatgtttgt ctccttatct tttttttgta tagtttacta
      661 aagtattaat atagttttaa gaagtaaata tttctaggtt gcaaaactta attgttcgtc
      721 tttatatgat catacccaaa atctttgatc cggaagaaat aaaggccatg tatttagtcc
      781 tggagttttg tttggttggt tttgtttgat tggttttgtt tttgtctggc ttttccaagc
      841 catcaccccc ttctccagtg tatatagttc tgttaatttt gccttttctc agcttttgaa
      901 atactgctca ctgttacttt gctctcattt ataaataaac caaaacataa ttatttcagt
      961 agtacatgta aatttgcttt tgaattttta atctttaata tacttacttt gtaaatgc
//
