LOCUS       NM_027016               3966 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus SEC62 homolog, preprotein translocation (Sec62),
            transcript variant 2, mRNA.
ACCESSION   NM_027016 XM_130832
VERSION     NM_027016.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Fumagalli,F., Noack,J., Bergmann,T.J., Cebollero,E., Pisoni,G.B.,
            Fasana,E., Fregno,I., Galli,C., Loi,M., Solda,T., D'Antuono,R.,
            Raimondi,A., Jung,M., Melnyk,A., Schorr,S., Schreiber,A.,
            Simonelli,L., Varani,L., Wilson-Zbinden,C., Zerbe,O., Hofmann,K.,
            Peter,M., Quadroni,M., Zimmermann,R. and Molinari,M.
  TITLE     Translocon component Sec62 acts in endoplasmic reticulum turnover
            during stress recovery
  JOURNAL   Nat Cell Biol 18 (11), 1173-1184 (2016)
   PUBMED   27749824
  REMARK    GeneRIF: identify Sec62 as a critical molecular component in
            maintenance and recovery of endoplasmic reticulum homeostasis
            Erratum:[Nat Cell Biol. 2016 Dec 23;19(1):76. doi:
            10.1038/ncb3451.. PMID: 28008182]
REFERENCE   3  (bases 1 to 3966)
  AUTHORS   Wemmert,S., Lindner,Y., Linxweiler,J., Wagenpfeil,S., Bohle,R.,
            Niewald,M. and Schick,B.
  TITLE     Initial evidence for Sec62 as a prognostic marker in advanced head
            and neck squamous cell carcinoma
  JOURNAL   Oncol Lett 11 (3), 1661-1670 (2016)
   PUBMED   26998059
REFERENCE   4  (bases 1 to 3966)
  AUTHORS   Jadhav,B., McKenna,M., Johnson,N., High,S., Sinning,I. and
            Pool,M.R.
  TITLE     Mammalian SRP receptor switches the Sec61 translocase from Sec62 to
            SRP-dependent translocation
  JOURNAL   Nat Commun 6, 10133 (2015)
   PUBMED   26634806
  REMARK    GeneRIF: Interaction of ribosomes and Sec62 with the core Sec61
            translocon is mutually exclusive.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3966)
  AUTHORS   Zheng,B., Zhao,D., Zhang,P., Shen,C., Guo,Y., Zhou,T., Guo,X.,
            Zhou,Z. and Sha,J.
  TITLE     Quantitative Proteomics Reveals the Essential Roles of Stromal
            Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in
            Mouse Testis
  JOURNAL   Mol Cell Proteomics 14 (10), 2682-2691 (2015)
   PUBMED   26199344
REFERENCE   6  (bases 1 to 3966)
  AUTHORS   Jung,S.J., Kim,J.E., Reithinger,J.H. and Kim,H.
  TITLE     The Sec62-Sec63 translocon facilitates translocation of the
            C-terminus of membrane proteins
  JOURNAL   J Cell Sci 127 (Pt 19), 4270-4278 (2014)
   PUBMED   25097231
REFERENCE   7  (bases 1 to 3966)
  AUTHORS   Reithinger,J.H., Kim,J.E. and Kim,H.
  TITLE     Sec62 protein mediates membrane insertion and orientation of
            moderately hydrophobic signal anchor proteins in the endoplasmic
            reticulum (ER)
  JOURNAL   J Biol Chem 288 (25), 18058-18067 (2013)
   PUBMED   23632075
REFERENCE   8  (bases 1 to 3966)
  AUTHORS   Lang,S., Benedix,J., Fedeles,S.V., Schorr,S., Schirra,C.,
            Schauble,N., Jalal,C., Greiner,M., Hassdenteufel,S., Tatzelt,J.,
            Kreutzer,B., Edelmann,L., Krause,E., Rettig,J., Somlo,S.,
            Zimmermann,R. and Dudek,J.
  TITLE     Different effects of Sec61alpha, Sec62 and Sec63 depletion on
            transport of polypeptides into the endoplasmic reticulum of
            mammalian cells
  JOURNAL   J Cell Sci 125 (Pt 8), 1958-1969 (2012)
   PUBMED   22375059
  REMARK    GeneRIF: The Sec62 protein plays a role in post-translational
            transport of presecretory proteins into endoplasmic reticulum.
REFERENCE   9  (bases 1 to 3966)
  AUTHORS   Lee,J., Xu,Y., Chen,Y., Sprung,R., Kim,S.C., Xie,S. and Zhao,Y.
  TITLE     Mitochondrial phosphoproteome revealed by an improved IMAC method
            and MS/MS/MS
  JOURNAL   Mol Cell Proteomics 6 (4), 669-676 (2007)
   PUBMED   17208939
REFERENCE   10 (bases 1 to 3966)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158135.5 and DV657309.1.
            
            On Jan 5, 2006 this sequence version replaced NM_027016.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088093.1, BC067202.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-498               AC158135.5         120693-121190
            499-1261            DV657309.1         1-763
            1262-3966           AC158135.5         146376-149080
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 14.29 cM"
     gene            1..3966
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="SEC62 homolog, preprotein translocation"
                     /db_xref="GeneID:69276"
                     /db_xref="MGI:MGI:1916526"
     exon            1..508
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             473..1669
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     translocation protein 1; translocation protein SEC62;
                     TP-1; SEC62 homolog"
                     /codon_start=1
                     /product="translocation protein SEC62 isoform 2"
                     /protein_id="NP_081292.1"
                     /db_xref="CCDS:CCDS17286.1"
                     /db_xref="GeneID:69276"
                     /db_xref="MGI:MGI:1916526"
                     /translation="MAERRRHKKRIQEVGEPSKEEKAVAKYLRFNCPTKSTNMMGHRV
                     DYFIASKAVECLLDSKWAKAKKGEDALFTTRESVVDYCNRLLKKQFFHRALKVMKMKY
                     DKDVKKEKDKGKSESGKEDDKKSKKESVKEEKTKKEKEKKKDGEKEDSKKEETPGTPK
                     KKETKKKFKLEPHDDQVFLDGNEVFVWIYDPVHIKTFVMGLILVIAVIAATLFPLWPA
                     EMRVGVYYLSVGAGCFVASILLLAIARCILFLIIWLITGGRHHFWFLPNLTADVGFID
                     SFRPLYTHEYKGPKADLKKDEKSETKKQQKSDSEEKSDSEKKEDEEGKGAPADHGPEG
                     SGGERHSDTDSDRREDDRSQHSSGNGNDFEMITKEELEQQTDGDCDEEDDDKDGEVPK
                     SAHEKS"
     exon            509..617
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            618..723
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            724..928
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1021
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1082
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1202
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..3966
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2428..2433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="hexamer: AATAAA"
     polyA_site      2447
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="major polyA site"
     regulatory      2772..2777
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="hexamer: AATAAA"
     regulatory      2781..2786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="hexamer: TATAAA"
     polyA_site      2792
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="major polyA site"
     polyA_site      2800
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
     regulatory      3941..3946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
                     /note="hexamer: AATAAA"
     polyA_site      3966
                     /gene="Sec62"
                     /gene_synonym="3100002M17Rik; Dtrp1; HTP1; Tloc1"
ORIGIN      
        1 gacgagctgt gtttaatgga gtcagactct gaagttgttt tccacctttt aaggggaact
       61 tggcgggact gaagtggggt gccacggggc tcgctggggc aggaagcgcc acaggtgacg
      121 ctgtgcgccc cggtggtccc ggcggcgtca gcgactctga ggggcggggc ccggggatct
      181 gtgtttacac gccagccagg tgactcgggc gttcagtcag ggctgagagc cgacgcgact
      241 tgccagcctc cagcgctctg actcacgtgg cgccggcctc cgggaccaga agcagaagca
      301 gcccgcgtcg tccgccaccg cacggacggc gggaagacca cggaggccgc ccccagccca
      361 gagcccgcgg cgggttacat aacgggccgc gagccgccgc gctctcgcga gatctcagcc
      421 gccccctccc cgactcgctc cacgtcagaa ggaaccgggc ggagcggcca acatggcgga
      481 acgcaggcga cacaagaagc ggatccagga agttggtgaa ccatctaaag aagagaaggc
      541 tgtagccaaa tatcttcgat tcaactgtcc aacaaaatcc accaacatga tgggtcatcg
      601 ggttgactat tttattgctt caaaagcagt ggagtgcctc ttggattcaa agtgggccaa
      661 ggccaagaaa ggagaggacg ctttatttac aaccagggag tctgtggtcg actactgcaa
      721 caggctttta aagaaacagt ttttccatcg ggccctaaaa gtaatgaaaa tgaaatatga
      781 taaagacgta aaaaaagaaa aagataaagg gaaatcagaa agtgggaaag aagatgataa
      841 aaagagcaag aaagaaagtg ttaaagaaga aaaaacaaaa aaggaaaaag aaaaaaagaa
      901 agatggtgaa aaggaagatt ccaaaaagga ggaaactcca ggaactccaa aaaagaagga
      961 aactaagaaa aaattcaaac ttgagccaca tgatgatcaa gtttttctgg atggaaatga
     1021 ggtttttgtg tggatctatg acccagttca cattaaaaca tttgtcatgg gattaattct
     1081 tgtgattgca gtaatagcag ccaccctctt ccctctctgg ccagcagaaa tgagagtagg
     1141 tgtttattac ctcagtgtgg gcgcaggctg ttttgtagcc agcattcttc tccttgctat
     1201 tgctcgctgc atcctgtttc tcatcatctg gctgataact ggaggaaggc accacttttg
     1261 gttcctgcca aacctgactg ctgacgtggg cttcattgac tccttcaggc ccctgtacac
     1321 acatgagtac aaaggaccaa aagcagactt aaagaaggat gagaaatctg aaaccaaaaa
     1381 gcagcagaag tccgacagtg aggaaaagtc agacagtgag aaaaaggagg atgaggaagg
     1441 gaaaggagct ccggcagacc atggaccaga aggctcgggc ggagagcggc actcagacac
     1501 ggacagtgac aggcgggaag acgaccgctc gcaacacagt agcgggaacg ggaatgattt
     1561 tgaaatgatc acgaaggagg agctggagca gcagacagat ggggattgtg atgaggagga
     1621 tgacgacaaa gatggagagg tgcctaaatc tgcacatgag aaatcataat ctgactagct
     1681 gggggactaa ataagtacag caggttgggt tttctgtgtt ggccgatcat catgatgtac
     1741 agatggcatt tgtagcattc tttaaatcca ttttctgaaa tgtatttgac attcagttac
     1801 agtcagcctt ttgttttgta gaacgttggt actgttattg gtacagttta aagccattaa
     1861 taagttttat ccatctgata attttacagt aagtaggcct cattttgata gtcatcagag
     1921 gtacattgtc cacaatgaca cttagaataa gggcattttt gatagttgta tggcttttta
     1981 ctgttagact aatggaaatt gctaaagaaa ctgcagcatt tcagattatg cataagtata
     2041 tagccacttg gcagtttctt taggatcaaa tgcttaaact cattgttctt gctagttatg
     2101 ctctttttgg gtttttaggt ttgtttggtt ttttgttttg ttttcataaa cttataccag
     2161 aaaaattcct tttttaaggt ttttgagtta gcagggatca agagcatttt gtggaaacaa
     2221 gccagctagt aacagtgcag caaccctttt aattcagcta cagattctcc ttttccagct
     2281 taggaaatcc caactgacct gtgcgttcaa tccagagaaa ggcggtcatc tccagcagaa
     2341 gtgaggagcg ctgactggac ggcgaggctc ttccttcctc cccatctcct tgtcataaca
     2401 taagatgtat tctgtacata atttacaaat aaaacatttt attttaattg ttacttatta
     2461 tttagacatt tctcaacact gaaatttgta aaacaaaaac catgtaaggt atgtttttag
     2521 aaagtttcag taacccacag aacatctgat ctttctacag cagcttcagt tgtgtgccaa
     2581 cattccatgt attttaatat gagtaaaagt tatccttatt aagtaagaac agacttaaag
     2641 tagtagctgt ttgtacgcct taatgttcat tgatttattt taaatcttta catttagaaa
     2701 tgggggctat gtcatcagac gaggaggcac tgctgtgaaa gacaatttgc tgttctaaaa
     2761 taccaattaa aaataaagga tataaaagaa aacacagcaa gccattggac tctaattgct
     2821 tcaaactagt ttggccattt actttttaca caccttcgct acaaaccttc tttaaaacgc
     2881 ctgcatatac acaagcaatc cttggagcca cgttttacac cccagtgagc agtgagtgat
     2941 tttcctttag tcacacggtg gttttgcttt gtagttagta ggtgatggat atataggtct
     3001 tcatcctcct ttaggctgtt tctcttcttg tgtttgtcca ttgtctgtat attgacagct
     3061 tatgaaatac agcttgactt gtttctttag tcttcctgtg tggatggaac ggtaggtgcg
     3121 gtcctcagag cgctgtgcat tgattacagt gcctcggtgt tcttccttca catttatttg
     3181 cagagccctg ctgcctcgtg tccacagtaa ggaagaggat aattacagct tatgagcttg
     3241 ttagtcacag gtagattaat acctatttag ggtatgaaaa tggaacaaga aactttgata
     3301 aaacaaaact gcttgtttga cctttactag tatagtaccc aggcactcaa tatttatttg
     3361 caagatcttt gtaacatatg tcctgtgtct agtctaaaca ataaagaatg ctagctacaa
     3421 agtccactta ctgatacaag tggatagtaa atttttcata agtatttgct gatctttggg
     3481 gagagtactc ttgttttcgt taggtatgga ctagaaattt cagcttaatt tctattgtat
     3541 ggttcaaggt aaaacacttt aaacaagtta aaaaatcaaa atgcttcctc tgttgtagtc
     3601 tttagacgtc tttgtgtcag agcatggaga attgtttaca ctagccaatg gagtagtgac
     3661 tcttaaaaaa tgatttccta tgctgctgta agtagaagca caaagctttt gggtaatgtg
     3721 gggtttacta tggagtgtat gtatgttggc ctatgcagga ttgaactaag caggaactga
     3781 gtccagatgg aagaatagta catatgtgaa tgttctcaaa attaatgtgt taaatagggt
     3841 gctttttctt ttccttccta cagaattctt aagtttttta aattataagt ccttctagag
     3901 aatgttaatt gataatttca acgaatacta cccagaaaat aataaatttt aatatattac
     3961 actgaa
//
