LOCUS       NM_153125               8754 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus SEC16 homolog A, endoplasmic reticulum export factor
            (Sec16a), mRNA.
ACCESSION   NM_153125
VERSION     NM_153125.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Wang,M.J., Wang,Y.C., Masson,E., Wang,Y.H., Yu,D., Qian,Y.Y.,
            Tang,X.Y., Deng,S.J., Hu,L.H., Wang,L., Wang,L.J., Rebours,V.,
            Cooper,D.N., Ferec,C., Li,Z.S., Chen,J.M., Zou,W.B. and Liao,Z.
  TITLE     SEC16A Variants Predispose to Chronic Pancreatitis by Impairing
            ER-to-Golgi Transport and Inducing ER Stress
  JOURNAL   Adv Sci (Weinh) 11 (38), e2402550 (2024)
   PUBMED   39119875
  REMARK    GeneRIF: SEC16A Variants Predispose to Chronic Pancreatitis by
            Impairing ER-to-Golgi Transport and Inducing ER Stress.
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Mayer,L., Jasztal,M., Pardo,M., Aguera de Haro,S., Collins,J.,
            Bariana,T.K., Smethurst,P.A., Grassi,L., Petersen,R., Nurden,P.,
            Favier,R., Yu,L., Meacham,S., Astle,W.J., Choudhary,J., Yue,W.W.,
            Ouwehand,W.H. and Guerrero,J.A.
  TITLE     Nbeal2 interacts with Dock7, Sec16a, and Vac14
  JOURNAL   Blood 131 (9), 1000-1011 (2018)
   PUBMED   29187380
REFERENCE   3  (bases 1 to 8754)
  AUTHORS   Wang,B., Iyengar,R., Li-Harms,X., Joo,J.H., Wright,C., Lavado,A.,
            Horner,L., Yang,M., Guan,J.L., Frase,S., Green,D.R., Cao,X. and
            Kundu,M.
  TITLE     The autophagy-inducing kinases, ULK1 and ULK2, regulate axon
            guidance in the developing mouse forebrain via a noncanonical
            pathway
  JOURNAL   Autophagy 14 (5), 796-811 (2018)
   PUBMED   29099309
REFERENCE   4  (bases 1 to 8754)
  AUTHORS   Bruno,J., Brumfield,A., Chaudhary,N., Iaea,D. and McGraw,T.E.
  TITLE     SEC16A is a RAB10 effector required for insulin-stimulated GLUT4
            trafficking in adipocytes
  JOURNAL   J Cell Biol 214 (1), 61-76 (2016)
   PUBMED   27354378
  REMARK    GeneRIF: SEC16A and RAB10 promote insulin-stimulated mobilization
            of GLUT4 from a perinuclear recycling endosome/trans Golgi network
            compartment.
REFERENCE   5  (bases 1 to 8754)
  AUTHORS   Cho,H.J., Yu,J., Xie,C., Rudrabhatla,P., Chen,X., Wu,J.,
            Parisiadou,L., Liu,G., Sun,L., Ma,B., Ding,J., Liu,Z. and Cai,H.
  TITLE     Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to
            allow ER-Golgi export
  JOURNAL   EMBO J 33 (20), 2314-2331 (2014)
   PUBMED   25201882
REFERENCE   6  (bases 1 to 8754)
  AUTHORS   Iinuma,T., Shiga,A., Nakamoto,K., O'Brien,M.B., Aridor,M.,
            Arimitsu,N., Tagaya,M. and Tani,K.
  TITLE     Mammalian Sec16/p250 plays a role in membrane traffic from the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 282 (24), 17632-17639 (2007)
   PUBMED   17428803
REFERENCE   7  (bases 1 to 8754)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 8754)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 8754)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK162931.1, CJ098463.1, BF456838.1, AA388295.1, CA463107.1,
            BU704044.1, CX236472.1, BQ950511.1, CO432077.1, CV883992.1,
            CK638146.1, AK122242.2, BC034649.1 and AI851539.1.
            
            On Feb 2, 2007 this sequence version replaced NM_153125.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784644.658199.1,
                                           SRR17253011.7159067.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-506               AK162931.1         2-507
            507-560             CJ098463.1         484-537
            561-823             BF456838.1         66-328
            824-1061            AK162931.1         825-1062
            1062-1157           AA388295.1         86-181
            1158-1755           CA463107.1         39-636
            1756-2191           BU704044.1         113-548
            2192-2797           CX236472.1         40-645
            2798-3346           BQ950511.1         207-755
            3347-3867           CO432077.1         119-639
            3868-4384           CV883992.1         146-662
            4385-4702           CK638146.1         345-662
            4703-7574           AK122242.2         140-3011
            7575-8734           BC034649.1         2693-3852
            8735-8754           AI851539.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 18.89 cM"
     gene            1..8754
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /note="SEC16 homolog A, endoplasmic reticulum export
                     factor"
                     /db_xref="GeneID:227648"
                     /db_xref="MGI:MGI:2139207"
     exon            1..142
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            143..3826
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..7285
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /EC_number="3.1.3.36"
                     /codon_start=1
                     /product="protein transport protein Sec16A"
                     /protein_id="NP_694765.2"
                     /db_xref="CCDS:CCDS38085.1"
                     /db_xref="GeneID:227648"
                     /db_xref="MGI:MGI:2139207"
                     /translation="MQPPPQAVPSGVAGPPPAGNPRSMFWANSPYRKPANNAPVAPIT
                     RPLQPVTDPFAFNRQTLQNTPVGSSSKSSLPNLPGPALSVFSQWPGLPVTPTNAGDSS
                     TGLHEPLSGTLSQPRADASLFPPASTPSSLPGLEVSRNAEADPSSGHEVQMLPHSAHY
                     IPGVGPEQPLGGQMNDSGSGPDQPMNRHAPHDGAVTHAASPFLPQPQMPGQWGPAQGG
                     PQPSYQHHSPYLEGPVQNMGLQAASLPHFPPPSSLHQGPGHESHAPQTFTPASLASGE
                     GNEIVHQQSKNHPLSSFPPKHTFEQNSRIGNMWASPELKQNPGVNKEHLLDPAHVNPF
                     TQGNSPENQAHHPPVAATNHALQEAASGALSMFFQGEETENEENLSSEKAGLDKRLNL
                     DSFSSTSRLGHPPPPGASGVYQAFPRGPSSEAAQEGDAQPYFSQSVGVRLDKQSTVPP
                     ANDAWGDVPGTGTRCASGPQCENVENLEFVQNQEVLPRETLSVDPFPLSDQIRYGPLP
                     GPAASRPATVGLTRGGGLNLEAPDTPLHPTRPDSVSSSYSSHSHRSPPGSARPQELVG
                     TFIQQEVGKLEDDTSGSFFKQIDSSPVGGETDEVTGSQNCCSSLSQPSTPSPPKPTGV
                     FQTSANSSFEPVKSHLVGVKPVEADRANMVVEVRGTQYCPKKRRAAVAPPDATSGNLE
                     QPPDNMETPCAPQACPLPLSTTGEAGQLVSNTAGTPLDTVRPVPDKRPSARAQGPVKC
                     ESPATTLWAQNELPDFGGNVLLAPAAPALYVPVKPKPSEVVHHPEKGMSGQKAWKQGS
                     VPPLQNQDPPGASENLENPPKVGEEEALPVQASSGYASLLSSPPTESLHNQPVLIAQP
                     DQSYNLAQPINFSVSLLNPNEKNQSWGDAVVGERSIVSNNWALGGDPEERAALSGVPA
                     SAVTGASLPSSIPQNCAPQGSGSSEMIASQSASWLVQQLSPQTPQSPHPNAEKGPSEF
                     VSSPAGNTSVMLVPPASSTLVPNSNKAKHSSNQEEAVGALDFTLNRTLENPVRMYSPS
                     PSDGPASQQPLPNHPRQSGPGLHNQDHFYQQVTKDAQDQHRLERAQPELVPPRPQNSP
                     QVPQASCPEPSNPESPPTQGQSESLAQPPASPASVNTGQLLPQPPQASSASVTSTNSS
                     QAAVRSEQLWLHPPPPNTFGPAPQDLASYYYYRPLYDAYQSQYPSPYPSDPGTASLYY
                     QDMYGLYEPRYRPYDSSASAYAENHRYSEPERPSSRASHYSDQLAPRQGYPEGYYNSK
                     SGWSSHSDYYANYYSGQYDYGDPSRWDRYYGSRLRDPRTWDRRYWYDSEHDPYRKDHY
                     AYSDRPEKCDDHWRYDPRFTGSFDDDAEIHRDPYGEEADRRSIHSEHSARSLRSTHSL
                     PSRRSSLSSHSHQSQIYRSHHVTGGSFEAPHAPGSFHGDYAYGTYASNFSGAHGFPEY
                     SYPADTSWPAVEQVPSRPTSPEKFTVPHVCARFGPGGQLLKVIPNLPSEGQPALVEIH
                     SLETLLQHTPEQEEMRSFPGPLGKDDTHKVDVINFAQNKATKCLQNESLIDKESASLL
                     WKFIILLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEES
                     GEAQLSFLTDSQTVTTSVLEKETERFRELLLYGRKKDALESAMKNGLWGHALLLASKM
                     DSRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAASTCCGDEKWGDWRPHLAMVLSN
                     LNNNMDVESRTMATMGDTLASKGLLDAAHFCYLMAQVGFGVYTKKTTKLVLIGSNHSL
                     PFLKFATNEAIQRTEAYEYAQSLGAHTCSLPNFQVFKFIYLCRLAEMGLATQAFHYCE
                     VIAKSVLTQPGAYSPVLISQLTQMASQLRLFDPQLKEKPEEESFVEPAWLVQLQHVER
                     QIQEGTVLWSQDGTEPQQCRITSGSEVEQSDGPGLNQQAGPQADNPLLMPSTEPLMHG
                     VQLLPTAPQTLPDGQPAHLSRVPMFPVPMSRGPLELSPAYGPPGSALGFPESSRSDPA
                     VLHPGQALPPTTLSLQESGLPPQEAKSPDPEMVPRGSPVRHSPPELSQEEFGESFADP
                     GSSRTAQDLETSPVWDLGSSSLTRAPSLTSDSEGKKPAQAVKKEPKEPKKTESWFSRW
                     LPGKKRTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEKKAPPPPPTSFPRVPQVAPTG
                     PAGPPTASVNVFSRKAGGSRARYVDVLNPSGTQRSEPALAPADFFAPLAPLPIPSNLF
                     VPNPDAEEPQPADGTGCRGQAPAGTQSKAESTLEPKVGSSTVSAPGPELLPSKPDGSQ
                     GGEAPGDHCPTGAPHGGSVPFYNPAQLVQASVTSGNSRPGRIGQRKYAALN"
     exon            3827..3963
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            3964..4061
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4062..4185
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4186..4384
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4385..4559
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4560..4702
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4764
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4765..4903
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            4904..5149
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5150..5247
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5248..5329
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5330..5447
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5448..5559
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5560..5665
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5666..5806
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5807..5920
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            5921..6104
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6105..6350
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6351..6433
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6434..6583
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6584..6661
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6662..6718
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6719..6830
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6831..6968
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            6969..7063
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            7064..7138
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            7139..7216
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     exon            7217..8739
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8708..8713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /note="hexamer: AATAAA"
     polyA_site      8739
                     /gene="Sec16a"
                     /gene_synonym="C230052J16Rik; p250"
                     /note="major polyA site"
ORIGIN      
        1 acgtcggcgg cggcacctgg agccggatgt gccaagatgg ctgcggctgc tgctgggaac
       61 gcgctagttc cccctactgc cgccgccgtc gccggctggg tttcagttcg cggcgcctga
      121 gaagccaacc acagctcaca ggcacaaatt aaagaaaagc tctactctgc ccccttgata
      181 agcagcatct ccttgtggaa aagactccaa catgcagcca ccacctcagg cagtcccgtc
      241 tggggtggct ggaccacccc cagctgggaa tcctcggagc atgttctggg ccaacagccc
      301 ttacaggaag ccagccaata atgcaccagt ggctcccata acacgcccat tgcagccagt
      361 aacggatccg tttgctttta atagacagac tcttcaaaac acaccagtag gaagttcatc
      421 caaaagcagc ctgcccaatt tgcctggccc agccctctct gttttttctc agtggcctgg
      481 tttgcctgtg actcccacaa atgctgggga tagctccaca ggattgcatg agcctctgtc
      541 aggaactctg tcacagccta gagcagatgc cagtctattt cctcctgcat ctaccccttc
      601 ttcactccct gggctggagg tgagcaggaa tgctgaggct gatcccagct caggacatga
      661 agtccagatg ctgccacatt cagctcacta cattccagga gtgggtcctg agcagcctct
      721 cgggggccaa atgaatgaca gtgggtccgg gcctgaccaa cccatgaata ggcatgcccc
      781 ccatgatggt gccgtgaccc acgcagcatc tcctttcctc cctcagccac agatgccagg
      841 tcagtggggg cctgcacaag gaggccctca gccctcctat cagcatcact caccctacct
      901 ggagggacct gttcagaaca tggggctcca ggctgccagc ttgccccatt ttccccctcc
      961 atctagtcta caccagggac ctggccatga gtctcatgcc ccacaaacat ttactccagc
     1021 atccttggcc agtggtgaag ggaatgagat agttcaccag caaagtaaaa atcacccatt
     1081 gagtagcttc ccccctaaac acacttttga gcagaattcc agaattggga atatgtgggc
     1141 aagcccagag ttgaagcaga atccgggagt gaataaagag catctgctag accctgccca
     1201 tgtcaatccc ttcactcagg gaaatagtcc tgaaaaccag gcgcatcatc ccccagtggc
     1261 agcaactaac catgccctgc aagaagcagc ctctggagct ctctcaatgt tttttcaagg
     1321 ggaagagaca gagaatgagg aaaatctctc atctgaaaaa gcaggcctcg ataagagatt
     1381 gaacttggat agcttttctt ctacctccag actgggccat ccaccaccac ctggagctag
     1441 tggtgtctac caggcctttc ctagaggccc tagcagtgag gctgcgcagg aaggagatgc
     1501 gcaaccttat ttttcgcagt ctgtgggcgt ccgtcttgac aaacagagca ctgtgccccc
     1561 tgctaatgat gcatggggtg atgttccggg gaccgggacc cgctgtgcca gtgggccaca
     1621 gtgtgagaat gttgagaact tagaatttgt tcagaatcaa gaagttttgc cccgtgagac
     1681 cctgagtgtg gatcccttcc ccctgagtga tcagatccga tatgggcccc ttcctgggcc
     1741 agctgcctcc aggcctgcta ctgtgggcct caccagaggt ggggggctaa atcttgaggc
     1801 cccagacaca ccactgcacc ctacacgacc tgacagtgtg tcatccagct acagcagcca
     1861 tagccacaga agtcctcctg ggtcagccag accccaagaa ttggtgggca cattcattca
     1921 gcaagaagtt gggaaacttg aagatgatac ttcagggagt ttttttaagc aaattgattc
     1981 ttctcctgtt ggaggtgaaa cagatgaggt gactgggagc caaaattgct gcagcagtct
     2041 gtcccagcct tcaaccccaa gccctccaaa acctacagga gtattccaaa caagtgcaaa
     2101 cagttctttt gagccagtaa aatcccactt ggttggagtg aaaccagttg aagcagatcg
     2161 tgccaacatg gtggttgaag tgagagggac ccaatactgt ccgaagaagc gcagggcagc
     2221 tgttgcacca cctgatgcta cctcaggaaa cctggaacag ccaccagaca acatggagac
     2281 cccgtgtgca cctcaggcct gccctttgcc tcttagcacc actggagagg ctgggcaact
     2341 ggtttcaaac acggcaggga cacccctgga tactgtgcgc ccagtgcctg ataagaggcc
     2401 ctcagccagg gcccagggac ctgtgaagtg tgagagccca gcaacaactc tgtgggcaca
     2461 aaatgagctg ccagattttg gtggcaatgt ccttctagcc cctgctgccc ctgcacttta
     2521 tgttcctgta aaaccaaaac cttcagaggt tgttcatcat ccagaaaagg gaatgtctgg
     2581 acagaaggcc tggaaacaag ggtctgtgcc acctttgcag aaccaagacc ctcctggtgc
     2641 ttctgagaac cttgagaacc ctcccaaggt aggagaagag gaggcccttc cagtgcaggc
     2701 aagttctggt tatgcgagtt tgctttcttc gccacccact gaatctttgc acaatcaacc
     2761 tgtcctgatt gctcagcctg atcaaagcta taatttggct cagcctatta atttttctgt
     2821 gtccttactg aatcctaatg agaagaatca gtcctgggga gatgctgtgg tgggggagag
     2881 atccatagtg agcaacaact gggctctcgg tggtgatcct gaagaacgtg ctgctctgtc
     2941 tggagttcca gccagtgctg tcactggcgc gtctttgcct agcagtatcc cccagaattg
     3001 tgcccctcaa ggttcggggt cttctgaaat gattgctagc cagtctgcca gttggttggt
     3061 tcaacagcta tcccctcaaa cccctcaaag tccgcatcca aatgcagaaa agggcccttc
     3121 cgagtttgtt agtagccctg ctggcaatac aagtgtgatg ctagttccac ctgcaagcag
     3181 tactttggtt cctaacagta ataaggcaaa gcactctagt aaccaggaag aagctgttgg
     3241 cgccctagac ttcacattaa atagaacttt ggaaaatcct gtgagaatgt acagcccatc
     3301 tccttcagat ggcccagctt ctcagcagcc tcttcccaat catcccagac aatctgggcc
     3361 tgggctgcat aaccaggatc atttctacca gcaggtaaca aaagatgctc aggaccagca
     3421 tagactagaa agagcccagc cagagctggt gcctcctcgg ccacagaatt ctccccaagt
     3481 gccccaagca tcgtgtccag agccttcaaa tcctgaaagc ccacccacac aaggacagtc
     3541 tgaaagcttg gcccagccac cagcaagtcc agcttcagtc aacacaggcc agctgctgcc
     3601 tcagccacca caggcttcca gtgcatccgt tacatctacc aactccagcc aggcagctgt
     3661 gcggtcagag cagctgtggc tacacccacc acctcctaat acttttggcc cagcacctca
     3721 agacttggca tcctactact attacagacc cctgtatgat gcctaccagt ctcagtaccc
     3781 ttcaccgtac ccgtcagatc ctgggacagc ctcgctgtac taccaggata tgtatggcct
     3841 gtatgagcct cgttacagac cctatgacag ctcagcatct gcttatgccg agaaccaccg
     3901 ctattctgag cctgagcggc ccagctcccg tgcaagtcac tattccgacc agcttgctcc
     3961 caggcaagga tatcctgaag gatactataa ttccaagagt ggatggagca gtcacagtga
     4021 ttactatgca aattattact ctggccagta tgattatgga gaccccagcc gctgggatcg
     4081 ttactatggg tctcgcctta gggatcctcg cacctgggac cggaggtact ggtatgactc
     4141 tgaacatgac ccatacagga aggaccacta tgcttacagt gacaggcctg agaaatgtga
     4201 tgatcactgg aggtatgacc ctcgcttcac tgggagcttc gacgatgacg ctgagatcca
     4261 cagggacccc tatggagaag aagcagacag acgcagcatc cacagtgagc actcggcacg
     4321 gagcctgcgc agcactcaca gtctgcccag ccgccgcagc agcctcagct cccattcaca
     4381 ccagagtcag atttacagaa gccaccatgt gactggcggt tcctttgagg cccctcatgc
     4441 cccaggctca tttcatggtg attatgccta tggcacatat gccagcaatt tcagcggtgc
     4501 ccatggtttt ccagagtaca gctaccctgc agacacctcc tggcctgctg tggagcaagt
     4561 tccatcaaga ccgacctctc ctgagaagtt tacggtgcct catgtctgtg ccaggtttgg
     4621 tcctggtggt cagctcctta aagtgattcc caacctgcct tcagaaggac agcctgcact
     4681 ggtggagatc cacagcttag agaccttgct gcagcacaca cctgagcagg aagagatgcg
     4741 ctccttccca ggacctctcg gcaaagatga tacccacaaa gtagatgtta ttaactttgc
     4801 acagaacaaa gcaacaaaat gtttgcagaa tgaaagttta attgataaag agtctgcaag
     4861 tcttctttgg aaattcatta ttctgttatg cagacagaat gggactgttg tgggaacaga
     4921 cattgcagag cttttgttac gggaccatag aactgtatgg cttcctggga agtcacccaa
     4981 tgaggcaaac ctgatagatt ttaccaatga ggctgtggaa caagtggaag aggaggagtc
     5041 aggggaggcc cagctctcat ttctcacaga cagtcagaca gtgaccacca gcgtgctgga
     5101 gaaggagact gagcggttcc gtgagctctt gctgtatggc cggaagaagg atgctctgga
     5161 gtctgccatg aaaaatggct tatggggtca tgctcttctg cttgcaagta agatggacag
     5221 ccggacacat gcccgtgtca tgaccaggtt tgccaacagt cttccaatca atgaccccct
     5281 gcagactgtc taccagctca tgtctggacg gatgcctgct gcatccacgt gttgtggcga
     5341 tgagaagtgg ggagattgga ggccacatct tgcaatggtt ttgtccaacc tgaacaacaa
     5401 catggatgtt gaatctagga ccatggctac gatgggtgat actctagcat caaaaggcct
     5461 gttggatgct gcacacttct gctacctgat ggcccaggtt ggatttgggg tttatacaaa
     5521 gaaaactaca aaacttgtct tgataggctc aaatcacagt ttgccctttt taaagtttgc
     5581 caccaatgaa gccattcaaa ggacggaggc ttatgagtat gctcagtccc ttggggcaca
     5641 tacctgttcc ttacctaact tccaggtgtt taaattcatc tacttgtgcc gcctggctga
     5701 aatgggactt gccacacagg ccttccacta ctgcgaagta atagccaaga gtgtcctgac
     5761 acagcctggt gcatactctc cagtactgat tagccagttg actcagatgg cttcccagtt
     5821 acgcctcttc gatcctcaac tgaaggagaa gccagaagag gagtcctttg tggagcctgc
     5881 ctggttggtg cagctgcagc acgtggagag gcagatccag gagggcactg tgctgtggag
     5941 ccaggatgga actgaacccc agcagtgtcg cattacatcg ggctctgagg tggagcagtc
     6001 agatggccct ggactcaacc agcaggcagg gccacaggcc gacaaccctc tactgatgcc
     6061 aagcactgag cctttgatgc atggcgtgca actgctgccc acagctcctc agacattgcc
     6121 tgatggccag cctgctcacc tttccagggt gccgatgttc ccagtgccaa tgtctcgtgg
     6181 gcccctggag ctgagtcctg cctatggacc cccaggatct gcacttggct tcccagaatc
     6241 ctccagatct gatcctgcag tgctgcatcc tgggcaggcc ctgccaccca ctacactaag
     6301 tctccaggaa agtgggcttc caccccagga ggccaaaagt ccagacccag aaatggtgcc
     6361 acggggctca cctgtcagac actcccctcc ggagctcagt caagaggagt ttggcgagag
     6421 cttcgctgac ccgggctctt ccagaacagc acaggacttg gaaacctccc cagtgtggga
     6481 tcttggcagc tccagtttga cacgtgcacc atctttgaca tctgattctg aagggaagaa
     6541 acctgcacag gctgtcaaaa aggagcccaa ggagcccaag aagactgagt cctggttctc
     6601 tcgttggctg cctggaaaaa aaaggacaga agcttatcta ccagatgaca agaacaaatc
     6661 aattgtttgg gatgaaaaga aaaaccagtg ggtgaatttg aatgaaccag aggaggagaa
     6721 gaaggctcca cccccacctc caacatcgtt ccccagggtt ccccaggtgg ctcccactgg
     6781 gcctgcagga ccacccacgg cctccgtgaa tgtgttttct agaaaagcag gtgggtccag
     6841 agctcgctat gtggatgttc taaacccaag tggaacacag cggagtgaac cagctcttgc
     6901 tcctgcagat ttctttgctc ctctcgcccc actgccaatt ccttctaact tatttgtacc
     6961 aaacccagat gcagaagagc cacaacctgc agatgggact ggctgtaggg gacaggcacc
     7021 agctgggact cagtctaagg cagagtccac cctggaaccc aaggtgggaa gttctacagt
     7081 atcagcccct ggacctgagc tcttaccctc caaaccagat ggctcccaag gaggagaggc
     7141 tcctggagac cattgtccta caggggcccc tcatggtggg tctgtgccct tttacaaccc
     7201 tgctcagttg gtgcaggctt ctgtcacctc gggaaattca aggccaggga ggattggcca
     7261 aagaaaatat gcggcattga actaggattg ctgctggatt cttatttgga accctgagat
     7321 gattagataa ctgttcttca ccaagaacca actagctgcc tcaggtctcg tggatcccag
     7381 atggacatgg cagtaactca gagccggtca tactgtgtgt ctccttcctt gcagcctatg
     7441 tatatgaatg attgaaaaat tgatttcaag gttcctttca aatgctggat agaatgaatc
     7501 taattcaaag tcttttatgt aaaagaacaa tgtttctttt ttagtcactt tagacaaatt
     7561 aaggtttgag ccacttgaga atatccccgc agagaggcta ggcagggatt cactacagga
     7621 gagcacagtt acttggacac caaggccttt ctcacctgag aaatggtgca aaccaaccag
     7681 ggatccttag gctactgaga aggctgtctg cttacctgga gaggactgac tgctgggatg
     7741 tctggcctaa gaggagcctc aaactgcact gggcaactgt gccttgtcct gtgaactggg
     7801 gagtacatgc cagctcctca aagggtcacc agatggcagc ggctcccagg ctggggagga
     7861 agtggttaca agggcatgtc tctccaacat ttccagcctc actccatttt ctgtggcctc
     7921 attagtgctg ggtgcgtttt ttttttgctc cgagctttcc gtcatggctc agtcactggg
     7981 gagagcccca gggagctgat attctgaagc tgctgatctg attaaccagg tcaaaaactg
     8041 gctgtgtgct gcaacgtact gccctcctag ccaggagtct gtatggagcc ctctagctga
     8101 ctcaatgtca cctgcagaca gcacttaaag ccttggtggt atcttggagt ggcttacggt
     8161 gtcctagcgc acactctgga accttgcatc tctaccccaa agcccaccag ggaccttcag
     8221 cagcgactca ctggctcacc catatcactt caaggagctg ccaccacata agagtcaaag
     8281 ctttaaggct tcactgactg ttcctaaaac tatccttaca ctctaaagag gaagggaaag
     8341 agagttaaat atttaaaaat cagcaggtag aaaacataaa tggtttgttt ggtattaaat
     8401 ttagaaattg tagaacatgg aattagtaca gcccttcccg gttttctggt tccttgcatc
     8461 atctaggaaa gactcacaga atggcttcca ggacgttcca tagatgagtg tacttggacc
     8521 ctcctgttcc tgtcattaag aaaggcctgt ccctgtgggt cagtagcaac ttgtgtcctt
     8581 gctgggacca ggcaggaccc ttgcctctgg ttcaaccctg cccaggctct gtgttgactg
     8641 ccaaccactt tggattcaag aagcttcctc cctgggagct gctatttttc caaaattcac
     8701 attgttaaat aaaatatttt gggctctcaa gtaacttaaa aaaaaaaaaa aaaa
//
