LOCUS       NM_198885               1136 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus scleraxis scleraxis bHLH transcription factor (Scx),
            mRNA.
ACCESSION   NM_198885 XM_354931
VERSION     NM_198885.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Bobzin,L., Nickle,A., Ko,S., Ince,M., Huang,A., Bhojwani,A.,
            Roberts,R. and Merrill,A.E.
  TITLE     FGFR2 directs inhibition of WNT signaling to regulate anterior
            fontanelle closure during skull development
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39775862
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Wada,S., Ideno,H., Nakashima,K., Komatsu,K., Demura,N.,
            Tomonari,H., Kimura,H., Tachibana,M. and Nifuji,A.
  TITLE     The histone H3K9 methyltransferase G9a regulates tendon formation
            during development
  JOURNAL   Sci Rep 14 (1), 20771 (2024)
   PUBMED   39237663
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1136)
  AUTHORS   Yambe,S., Yoshimoto,Y., Ikeda,K., Maki,K., Takimoto,A.,
            Tokuyama,A., Higuchi,S., Yu,X., Uchibe,K., Miura,S., Watanabe,H.,
            Sakuma,T., Yamamoto,T., Tanimoto,K., Kondoh,G., Kasahara,M.,
            Mizoguchi,T., Docheva,D., Adachi,T. and Shukunami,C.
  TITLE     Sclerostin modulates mineralization degree and stiffness profile in
            the fibrocartilaginous enthesis for mechanical tissue integrity
  JOURNAL   Front Cell Dev Biol 12, 1360041 (2024)
   PUBMED   38895158
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1136)
  AUTHORS   Levay,A.K., Peacock,J.D., Lu,Y., Koch,M., Hinton,R.B. Jr.,
            Kadler,K.E. and Lincoln,J.
  TITLE     Scleraxis is required for cell lineage differentiation and
            extracellular matrix remodeling during murine heart valve formation
            in vivo
  JOURNAL   Circ Res 103 (9), 948-956 (2008)
   PUBMED   18802027
  REMARK    GeneRIF: Scleraxis is required for cell lineage differentiation and
            extracellular matrix remodeling during murine heart valve formation
            in vivo.
REFERENCE   5  (bases 1 to 1136)
  AUTHORS   Pryce,B.A., Brent,A.E., Murchison,N.D., Tabin,C.J. and
            Schweitzer,R.
  TITLE     Generation of transgenic tendon reporters, ScxGFP and ScxAP, using
            regulatory elements of the scleraxis gene
  JOURNAL   Dev Dyn 236 (6), 1677-1682 (2007)
   PUBMED   17497702
REFERENCE   6  (bases 1 to 1136)
  AUTHORS   Perez,A.V., Perrine,M., Brainard,N. and Vogel,K.G.
  TITLE     Scleraxis (Scx) directs lacZ expression in tendon of transgenic
            mice
  JOURNAL   Mech Dev 120 (10), 1153-1163 (2003)
   PUBMED   14568104
REFERENCE   7  (bases 1 to 1136)
  AUTHORS   Asou,Y., Nifuji,A., Tsuji,K., Shinomiya,K., Olson,E.N., Koopman,P.
            and Noda,M.
  TITLE     Coordinated expression of scleraxis and Sox9 genes during embryonic
            development of tendons and cartilage
  JOURNAL   J Orthop Res 20 (4), 827-833 (2002)
   PUBMED   12168674
REFERENCE   8  (bases 1 to 1136)
  AUTHORS   Schweitzer,R., Chyung,J.H., Murtaugh,L.C., Brent,A.E., Rosen,V.,
            Olson,E.N., Lassar,A. and Tabin,C.J.
  TITLE     Analysis of the tendon cell fate using Scleraxis, a specific marker
            for tendons and ligaments
  JOURNAL   Development 128 (19), 3855-3866 (2001)
   PUBMED   11585810
REFERENCE   9  (bases 1 to 1136)
  AUTHORS   Cserjesi,P., Brown,D., Ligon,K.L., Lyons,G.E., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A. and Olson,E.N.
  TITLE     Scleraxis: a basic helix-loop-helix protein that prefigures
            skeletal formation during mouse embryogenesis
  JOURNAL   Development 121 (4), 1099-1110 (1995)
   PUBMED   7743923
REFERENCE   10 (bases 1 to 1136)
  AUTHORS   Burgess,R., Cserjesi,P., Ligon,K.L. and Olson,E.N.
  TITLE     Paraxis: a basic helix-loop-helix protein expressed in paraxial
            mesoderm and developing somites
  JOURNAL   Dev Biol 168 (2), 296-306 (1995)
   PUBMED   7729571
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC157566.6 and AV162294.1.
            
            On Feb 18, 2010 this sequence version replaced NM_198885.2.
            
            Summary: This gene encodes a basic helix-loop-helix type
            transcription factor involved in mesoderm and heart valve
            formation. The encoded protein is expressed during embryonic
            development of tendons and ligaments. The gene product regulates
            collagen type I gene expression in cardiac fibroblasts and
            myofibroblasts, and it may play a role in myocardial remodeling.
            The protein is expressed in the scar area of the adult heart
            following myocardial infarction. [provided by RefSeq, Feb 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062161.1, CB204742.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-749               AC157566.6         178201-178949       c
            750-1129            AC157566.6         176926-177305       c
            1130-1136           AV162294.1         257-263
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.92 cM"
     gene            1..1136
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /note="scleraxis scleraxis bHLH transcription factor"
                     /db_xref="GeneID:20289"
                     /db_xref="MGI:MGI:102934"
     exon            1..749
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..788
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /note="scleraxis"
                     /codon_start=1
                     /product="basic helix-loop-helix transcription factor
                     scleraxis"
                     /protein_id="NP_942588.1"
                     /db_xref="CCDS:CCDS27571.1"
                     /db_xref="GeneID:20289"
                     /db_xref="MGI:MGI:102934"
                     /translation="MSFAMLRSAPPPGRYLYPEVSPLSEDEDRGSESSGSDEKPCRVH
                     AARCGLQGARRRAGGRRAAGSGPGPGGRPGREPRQRHTANARERDRTNSVNTAFTALR
                     TLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHSGPAFFHSGRAGS
                     PLPPPPPPPPLARDGGENTQPKQICTFCLSNQRKLSKDRDRKTAIRS"
     exon            750..1136
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1109..1114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /note="hexamer: AATGAA"
     polyA_site      1129
                     /gene="Scx"
                     /gene_synonym="Bhlha41; Scl"
                     /note="major polyA site"
ORIGIN      
        1 ctcggctggc ggccccactc cagtccgaac acatgtgccc gcctcacccg gcagccgcca
       61 ccgcggggcg cagcggagac ccctggcccg gcggcctgtg gggacctaaa gaggcggcat
      121 gagcagcgca cggtggagct gacgccgcgc cccctgcccg gcccatgtcc ttcgccatgc
      181 tgcgttcagc gccgccgccg ggtcgctacc tgtaccctga ggtgagcccg ctgtcggagg
      241 atgaggaccg cggaagcgag agctcgggct ccgacgagaa accctgccgt gtgcatgctg
      301 cgcgctgtgg cctccagggc gcccggcggc gggcaggagg acggagggcc gcgggtagcg
      361 ggccaggacc cggggggcgg ccaggccgcg agccccggca gcggcacaca gcgaatgcgc
      421 gcgagcggga ccgcaccaac agcgtgaaca cggccttcac tgcgctgcgc acactcatcc
      481 ccaccgagcc agcggaccgc aagctctcca agattgagac gctgcgcctg gcctccagct
      541 acatttctca cctgggcaat gtgctgctgg tgggtgaggc ctgtggcgac gggcaaccat
      601 gccactcggg acccgctttc ttccacagcg gtcgtgcggg cagcccactg ccaccgccgc
      661 cgccgccacc accactggcc agagacggcg gcgagaacac ccagcccaaa cagatctgca
      721 ccttctgcct cagcaaccag agaaagttga gcaaagaccg tgacagaaag acggcgattc
      781 gaagttagaa ggaggagggt ccacagcagc caagaggtga tgccactagt gaaggtggat
      841 gccactgaag agtcacggag agccccactt tggacctgat ctgagcgcag gtccccaaga
      901 gcacgctcac caggccagac ctgccccagc tatgagcagg gcctatgaac agagagatgg
      961 acaggcgggt gatggcttga ctctaagcag ccgaacactg acccaggccc actgggactt
     1021 tccatgctgg ctctctacct gggccaagtg ttggcatctt gtgtctttaa tatgataata
     1081 taaagtctgg aaattttgta taattaaaaa tgaaacagta tcttccaaat tgaggc
//
