LOCUS       NM_020052               3576 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Mus musculus signal peptide, CUB domain, EGF-like 2 (Scube2), mRNA.
ACCESSION   NM_020052
VERSION     NM_020052.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Amato,C.M., Xu,X. and Yao,H.H.
  TITLE     An extragenital cell population contributes to urethra closure
            during mouse penis development
  JOURNAL   Sci Adv 10 (49), eadp0673 (2024)
   PUBMED   39642224
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Shi,T., Yue,S., Xie,C., Li,X., Yang,D., Hu,L., Zhong,Y., Zhang,Y.
            and Liu,W.
  TITLE     MMP-2-mediated Scube2 degradation promotes blood-brain barrier
            disruption by blocking the interaction between astrocytes and
            endothelial cells via inhibiting Sonic hedgehog pathway during
            early cerebral ischemia
  JOURNAL   J Neurochem 168 (9), 1877-1894 (2024)
   PUBMED   38148633
  REMARK    GeneRIF: MMP-2-mediated Scube2 degradation promotes blood-brain
            barrier disruption by blocking the interaction between astrocytes
            and endothelial cells via inhibiting Sonic hedgehog pathway during
            early cerebral ischemia.
REFERENCE   3  (bases 1 to 3576)
  AUTHORS   Tsukui,T., Wolters,P.J. and Sheppard,D.
  TITLE     Alveolar fibroblast lineage orchestrates lung inflammation and
            fibrosis
  JOURNAL   Nature 631 (8021), 627-634 (2024)
   PUBMED   38987592
REFERENCE   4  (bases 1 to 3576)
  AUTHORS   Lin,Y.C., Niceta,M., Muto,V., Vona,B., Pagnamenta,A.T.,
            Maroofian,R., Beetz,C., van Duyvenvoorde,H., Dentici,M.L.,
            Lauffer,P., Vallian,S., Ciolfi,A., Pizzi,S., Bauer,P.,
            Gruning,N.M., Bellacchio,E., Del Fattore,A., Petrini,S.,
            Shaheen,R., Tiosano,D., Halloun,R., Pode-Shakked,B., Albayrak,H.M.,
            Isik,E., Wit,J.M., Dittrich,M., Freire,B.L., Bertola,D.R.,
            Jorge,A.A.L., Barel,O., Sabir,A.H., Al Tenaiji,A.M.J., Taji,S.M.,
            Al-Sannaa,N., Al-Abdulwahed,H., Digilio,M.C., Irving,M.,
            Anikster,Y., Bhavani,G.S.L., Girisha,K.M., Haaf,T., Taylor,J.C.,
            Dallapiccola,B., Alkuraya,F.S., Yang,R.B. and Tartaglia,M.
  CONSRTM   Genomics England Research Consortium
  TITLE     SCUBE3 loss-of-function causes a recognizable recessive
            developmental disorder due to defective bone morphogenetic protein
            signaling
  JOURNAL   Am J Hum Genet 108 (1), 115-133 (2021)
   PUBMED   33308444
REFERENCE   5  (bases 1 to 3576)
  AUTHORS   Lin,Y.C., Liu,C.Y., Kannagi,R. and Yang,R.B.
  TITLE     Inhibition of Endothelial SCUBE2 (Signal Peptide-CUB-EGF
            Domain-Containing Protein 2), a Novel VEGFR2 (Vascular Endothelial
            Growth Factor Receptor 2) Coreceptor, Suppresses Tumor Angiogenesis
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (5), 1202-1215 (2018)
   PUBMED   29545238
REFERENCE   6  (bases 1 to 3576)
  AUTHORS   Xavier,G.M., Economou,A., Senna Guimaraes,A.L., Sharpe,P.T. and
            Cobourne,M.T.
  TITLE     Characterization of a mouse Scube3 reporter line
  JOURNAL   Genesis 48 (12), 684-692 (2010)
   PUBMED   20957652
REFERENCE   7  (bases 1 to 3576)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
REFERENCE   8  (bases 1 to 3576)
  AUTHORS   Grimmond,S., Larder,R., Van Hateren,N., Siggers,P., Morse,S.,
            Hacker,T., Arkell,R. and Greenfield,A.
  TITLE     Expression of a novel mammalian epidermal growth factor-related
            gene during mouse neural development
  JOURNAL   Mech Dev 102 (1-2), 209-211 (2001)
   PUBMED   11287194
REFERENCE   9  (bases 1 to 3576)
  AUTHORS   Amid,C., Bahr,A., Mujica,A., Sampson,N., Bikar,S.E.,
            Winterpacht,A., Zabel,B., Hankeln,T. and Schmidt,E.R.
  TITLE     Comparative genomic sequencing reveals a strikingly similar
            architecture of a conserved syntenic region on human chromosome
            11p15.3 (including gene ST5) and mouse chromosome 7
  JOURNAL   Cytogenet Cell Genet 93 (3-4), 284-290 (2001)
   PUBMED   11528127
REFERENCE   10 (bases 1 to 3576)
  AUTHORS   Cichutek,A., Brueckmann,T., Seipel,B., Hauser,H., Schlaubitz,S.,
            Prawitt,D., Hankeln,T., Schmidt,E.R., Winterpacht,A. and Zabel,B.U.
  TITLE     Comparative architectural aspects of regions of conserved synteny
            on human chromosome 11p15.3 and mouse chromosome 7 (including genes
            WEE1 and LMO1)
  JOURNAL   Cytogenet Cell Genet 93 (3-4), 277-283 (2001)
   PUBMED   11528126
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165959.5, AK142950.1, AC132584.5, AK016571.1 and CO044579.1.
            
            On Jan 5, 2010 this sequence version replaced NM_020052.1.
            
            CCDS Note: The coding region has been updated to represent an
            alternate splice pattern that is more supported by the available
            transcript data. The update results in a shorter protein that
            maintains the original reading frame.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142950.1, SRR7345562.3018055.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC165959.5         188724-188757       c
            35-394              AK142950.1         2-361
            395-395             AC165959.5         180673-180673       c
            396-710             AK142950.1         362-676
            711-711             AC132584.5         221761-221761       c
            712-937             AK142950.1         678-903
            938-2086            AK016571.1         85-1233
            2087-3533           AK142950.1         2054-3500
            3534-3555           CO044579.1         22-43               c
            3556-3556           AC132584.5         173516-173516       c
            3557-3576           CO044579.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 57.53 cM"
     gene            1..3576
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /note="signal peptide, CUB domain, EGF-like 2"
                     /db_xref="GeneID:56788"
                     /db_xref="MGI:MGI:1928765"
     exon            1..220
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..3000
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /note="CUB domain and EGF-like repeat containing 1; signal
                     peptide, CUB and EGF-like domain-containing protein 2;
                     scube/You"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 2 precursor"
                     /protein_id="NP_064436.2"
                     /db_xref="CCDS:CCDS21740.2"
                     /db_xref="GeneID:56788"
                     /db_xref="MGI:MGI:1928765"
                     /translation="MGVAGCGRPREARALLLLLLLLPPLLAAAVPPDRGLTNGPSEDV
                     DECAQGLDDCHADALCQNTPTSYKCSCKPGYQGEGRQCEDMDECDNTLNGGCVHDCLN
                     IPGNYRCTCFDGFMLAHDGHNCLDMDECLENNGGCQHICTNVIGSYECRCKEGFFLSD
                     NQHTCIHRSEEGLSCMNKDHGCGHICKEAPRGSVACECRPGFELAKNQKDCILTCNHG
                     NGGCQHSCEDTAEGPECSCHPRYRLHADGRSCLEQEGTVLEGTESNATSVADGDKRVK
                     RRLLMETCAVNNGGCDRTCKDTSTGVHCSCPTGFTLQVDGKTCKDIDECQTRNGGCNH
                     FCKNTVGSFDCSCKKGFKLLTDEKSCQDVDECSLERTCDHSCINHPGTFICACNPGYT
                     LYSFTHCGDTNECSVNNGGCQQVCINTVGSYECQCHPGFKLHWNKKDCVEVKGFPPTS
                     MTPRVSLHCGKSGGGDRCFLRCRSGIHLSSGLQEAYSVTCGSSSLRSKQQKSNDSAFG
                     DVVTVRTSVTFKLNEGKCSLQKAKLSPEGLRPALPERHSSVKESFQYANLTCSPGKQV
                     PGALGRLNAPKEMFITVEFERETYEKEVTASCNLSCVVKRTEKRLRKALRTLKRAAHR
                     EQFHLQLSGMDLDMAKTPSRVSGQHEETCGVGQGHEESQCGLCQPGEYSANGFAPCQL
                     CALGTFQPDVGRTSCLSCGGGLPTKHLGATSFQDCETRVQCSPGHFYNTTTHRCIRCP
                     LGTYQPEFGKNNCVSCPGNTTTDFDGSTNITQCKNRKCGGELGDFTGYIESPNYPGNY
                     PANSECTWTINPPPKRRILIVVPEIFLPIEDDCGDYLVMRKTSSSNSVTTYETCQTYE
                     RPIAFTSRSKKLWIQFKSNEGNSARGFQVPYVTYDEDYQELIEDIVRDGRLYASENHQ
                     EILKDKKLIKALFDVLAHPQNYFKYTAQESREMFPRSFIRLLRSKVSRFLRPYK"
     exon            221..343
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            344..469
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            470..604
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..730
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            731..847
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            848..937
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1054
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1177
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1294
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1417
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1534
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1618
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1723
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1879
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2086
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2254
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2416
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2614
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2615..2767
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2847
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3563
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3137..3142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /note="hexamer: AATACA"
     polyA_site      3161
                     /gene="Scube2"
                     /gene_synonym="4932442O19Rik; Cegf1; Cegp1"
                     /note="major polyA site"
ORIGIN      
        1 gggggtgtcg cgggcgcaga ggccgggagc ccgcacacgc ctccccgcgc cgctgccgcc
       61 gcaggctcta cgcggctctg cgctgcggct gccatggggg tcgcgggttg cggtcggcct
      121 cgggaagccc gggcgctgtt gctgctgctg ctgctgctgc cgccgctgct ggccgccgcc
      181 gtccctcccg accggggtct caccaacggg ccgtccgagg atgtggatga gtgtgcccaa
      241 gggctcgatg actgccatgc tgatgcactg tgtcagaaca cacccacttc ctacaagtgc
      301 tcttgcaagc ctggctacca aggggaaggc aggcagtgtg aggacatgga tgaatgtgac
      361 aacacactca acggaggctg tgtccacgac tgtttaaata ttccaggcaa ctatcgctgc
      421 acctgtttcg atggcttcat gttggctcat gatggtcata attgtcttga tatggacgaa
      481 tgcttggaga acaatggtgg ctgccagcac atctgcacca acgttattgg gagctatgaa
      541 tgtcgctgca aagaaggttt ctttctaagt gacaatcagc acacttgcat tcaccgctca
      601 gaagaaggcc tgagctgtat gaacaaggat catggctgtg gtcacatctg caaggaggcc
      661 ccaagaggca gcgttgcctg tgaatgcagg cctggttttg aactggccaa aaaccagaaa
      721 gactgcatct tgacttgtaa ccatggaaat ggtgggtgtc agcactcctg tgaagataca
      781 gcagaaggcc ctgagtgtag ttgccatcca cggtataggt tgcatgctga tgggagaagc
      841 tgcctagagc aagagggcac tgtcttggag gggacagaga gcaatgccac atcagtggcg
      901 gatggagata aacgggtgaa gcgtcggctg ctcatggaaa cgtgtgccgt caacaacgga
      961 ggctgcgacc gcacctgtaa ggacacgtcc acaggcgtcc actgcagctg ccccactgga
     1021 ttcactctcc aggtggatgg gaagacatgt aaagatatcg atgagtgcca gacccggaat
     1081 ggaggttgta accatttctg caaaaacacc gtgggcagtt ttgactgcag ttgcaaaaaa
     1141 ggatttaaat tactaacaga cgagaagtct tgccaagatg tggatgaatg ttcgttggaa
     1201 aggacctgtg accacagttg catcaaccac cctggcacat ttatttgtgc ctgcaaccca
     1261 gggtacactc tctatagctt cacccactgt ggagacacca acgagtgcag tgtcaacaat
     1321 ggaggctgcc agcaagtgtg catcaacaca gtgggtagct acgaatgtca gtgtcaccca
     1381 gggttcaaac tccactggaa taaaaaagac tgtgtggaag tgaagggctt cccgcccact
     1441 agcatgacac cgcgtgtgtc cctgcactgt ggtaaaagtg gtggaggaga caggtgcttc
     1501 ctcagatgtc gctctggcat tcacctctcg tcaggactgc aagaggctta ctctgtcacc
     1561 tgtggctctt cctctctcag aagcaaacag caaaaatcaa atgactctgc ctttggggat
     1621 gtcgtcaccg tcaggacaag tgtaaccttt aagctaaatg aaggcaagtg tagtttgcaa
     1681 aaggccaagc tgtctcccga gggtctacga ccagcactac cagagaggca cagctcagta
     1741 aaagagagct tccagtacgc aaaccttaca tgcagccctg gcaagcaagt cccgggagcc
     1801 cttggccgac tgaacgcccc taaggaaatg tttatcacgg ttgagtttga acgggaaact
     1861 tacgaaaagg aggtgacagc ttcctgcaat ctgagctgcg tggtaaagcg gacagagaag
     1921 agactgcgga aagctctccg cacactcaag agagctgccc acagggagca gtttcatctg
     1981 cagctctcag gcatggacct cgatatggct aaaacgccct ccagagtgtc aggacagcat
     2041 gaagagacct gtggagtggg acagggtcat gaagaaagcc agtgtggcct ttgtcagcca
     2101 ggtgaatatt cagcaaatgg atttgcccct tgccagctct gtgccctggg cacctttcag
     2161 cctgatgtgg gccgtacatc ttgcctctca tgcggaggag gtcttcccac caaacatctg
     2221 ggagccactt ccttccagga ttgtgaaacc cgagttcaat gctcgcctgg gcatttctac
     2281 aacaccacca cacaccgatg tatccgatgc ccactaggga cataccagcc tgaatttgga
     2341 aaaaataatt gtgtttcctg cccgggcaat actaccaccg actttgatgg ctccaccaac
     2401 ataacacagt gtaaaaacag aaagtgtgga ggggagctgg gggatttcac agggtatatt
     2461 gaatccccaa actaccctgg gaactaccca gccaactcag agtgcacctg gaccatcaac
     2521 ccacccccca aacgccggat cctgattgtg gtccctgaga tcttcctgcc catagaggat
     2581 gactgcggtg actacctggt gatgcggaaa acctcttcat caaattctgt gaccacatat
     2641 gaaacctgcc agacttatga acgccccatc gccttcacct ccaggtcaaa gaaactgtgg
     2701 attcagttca agtccaatga agggaacagt gcccgggggt tccaggttcc ctatgtgaca
     2761 tacgatgagg actaccagga gctcattgaa gacatagttc gagatggcag gctctatgcc
     2821 tctgagaacc atcaggaaat actgaaggat aagaaactga tcaaggctct gtttgatgtc
     2881 ctggcacatc cccagaacta tttcaagtac acagcccagg agtcccgaga gatgtttcca
     2941 agatctttca tccgattgct acgttccaaa gtgtcaaggt ttctgaggcc ttacaaatga
     3001 ctcttcccac acgccactgt ggacagggtc ctactgtggg ctcagtgggc tgcagccggt
     3061 ttccctccac atgccgcctg ccatcggatt attaccacca gtaccagtga ctcgtcagag
     3121 ttcagttttt atagacaata cagatatctt gataaactga acttgggttt tctttcccaa
     3181 catttcagat gtggactgcg tacgctttga gtagcactgt gtgggcacat gtcctggatg
     3241 ttgtgagggc tgactgaact ggcctccttt cgggttctca ctcctccgac tgtctgtatt
     3301 ggaaaggagg ccacagaaag gctactctgg cttcatcacc atatcctcca ggccagtcct
     3361 ctcccaggaa acatctgtac tcatcagagg tgaccaggca acaggcaagc aggagggaag
     3421 gacaagccac tcaggccttc tgagtcacgg gaagactcag gtcctcccag ccttccacag
     3481 cctctgcaat gccagtttaa tcctaactgt gtgagttcta aacagtgatg attaaaaaaa
     3541 aaaaaaaaaa aaaagactag aaaaaaaaaa aaaaaa
//
