LOCUS       NM_022723               7842 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus signal peptide, CUB domain, EGF-like 1 (Scube1),
            transcript variant 2, mRNA.
ACCESSION   NM_022723
VERSION     NM_022723.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Sahoo,B.K., Lin,Y.C., Tu,C.F., Lin,C.C., Liao,W.J., Li,F.A.,
            Li,L.H., Mou,K.Y., Roffler,S.R., Wang,S.P., Yeh,C.T., Yao,C.Y.,
            Hou,H.A., Chou,W.C., Tien,H.F. and Yang,R.B.
  TITLE     Signal peptide-CUB-EGF-like repeat-containing protein 1-promoted
            FLT3 signaling is critical for the initiation and maintenance of
            MLL-rearranged acute leukemia
  JOURNAL   Haematologica 108 (5), 1284-1299 (2023)
   PUBMED   36005562
  REMARK    GeneRIF: Signal peptide-CUB-EGF-like repeat-containing protein
            1-promoted FLT3 signaling is critical for the initiation and
            maintenance of MLL-rearranged acute leukemia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7842)
  AUTHORS   Qiu,T., Huteckova,B., Seppala,M., Cobourne,M.T., Chen,Z.,
            Hovorakova,M., Buchtova,M. and Tucker,A.S.
  TITLE     Molecular profiling of the vestibular lamina highlights a key role
            for Hedgehog signalling
  JOURNAL   Development 150 (7) (2023)
   PUBMED   36971701
REFERENCE   4  (bases 1 to 7842)
  AUTHORS   Grimaldi,A., Comai,G., Mella,S. and Tajbakhsh,S.
  TITLE     Identification of bipotent progenitors that give rise to myogenic
            and connective tissues in mouse
  JOURNAL   Elife 11, e70235 (2022)
   PUBMED   35225230
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7842)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7842)
  AUTHORS   Xavier,G.M., Panousopoulos,L. and Cobourne,M.T.
  TITLE     Scube3 is expressed in multiple tissues during development but is
            dispensable for embryonic survival in the mouse
  JOURNAL   PLoS One 8 (1), e55274 (2013)
   PUBMED   23383134
  REMARK    Erratum:[PLoS One. 2013;8(9).
            doi:10.1371/annotation/b65bdf5d-4917-4936-9d9d-f217ab2602cd]
REFERENCE   7  (bases 1 to 7842)
  AUTHORS   Zhuang,J., Deane,J.A., Yang,R.B., Li,J. and Ricardo,S.D.
  TITLE     SCUBE1, a novel developmental gene involved in renal regeneration
            and repair
  JOURNAL   Nephrol Dial Transplant 25 (5), 1421-1428 (2010)
   PUBMED   20042401
  REMARK    GeneRIF: SCUBE1 may be involved in the regulation of tubular cell
            proliferation and re-epithelialization during the resolution of
            kidney injury.
REFERENCE   8  (bases 1 to 7842)
  AUTHORS   Xavier,G.M., Sharpe,P.T. and Cobourne,M.T.
  TITLE     Scube1 is expressed during facial development in the mouse
  JOURNAL   J Exp Zool B Mol Dev Evol 312B (5), 518-524 (2009)
   PUBMED   19101956
  REMARK    GeneRIF: During tooth development, Scube1 localized to the dental
            papilla of both incisor and molar teeth, suggestina a role for
            Scube1 during early craniofacial development.
REFERENCE   9  (bases 1 to 7842)
  AUTHORS   Tu,C.F., Yan,Y.T., Wu,S.Y., Djoko,B., Tsai,M.T., Cheng,C.J. and
            Yang,R.B.
  TITLE     Domain and functional analysis of a novel platelet-endothelial cell
            surface protein, SCUBE1
  JOURNAL   J Biol Chem 283 (18), 12478-12488 (2008)
   PUBMED   18303018
  REMARK    GeneRIF: These results support the dual roles of SCUBE1 on brain
            morphogenesis and cell-cell adhesions through its distinct domain
            function.
REFERENCE   10 (bases 1 to 7842)
  AUTHORS   Grimmond,S., Larder,R., Van Hateren,N., Siggers,P., Hulsebos,T.J.,
            Arkell,R. and Greenfield,A.
  TITLE     Cloning, mapping, and expression analysis of a gene encoding a
            novel mammalian EGF-related protein (SCUBE1)
  JOURNAL   Genomics 70 (1), 74-81 (2000)
   PUBMED   11087664
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ104466.1, AK041590.1, BC080278.1 and AL583887.9.
            
            On Sep 28, 2012 this sequence version replaced NM_022723.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon, compared to variant 1. The encoded isoform (b) is shorter
            than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            inferred exon combination        :: based on alignments, homology
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                CJ104466.1         2-19
            19-2865             AK041590.1         2-2848
            2866-3726           BC080278.1         2591-3451
            3727-7842           AL583887.9         120950-125065       c
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.4 cM"
     gene            1..7842
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /note="signal peptide, CUB domain, EGF-like 1"
                     /db_xref="GeneID:64706"
                     /db_xref="MGI:MGI:1890616"
     exon            1..221
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..3100
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; signal peptide, CUB and EGF-like
                     domain-containing protein 1"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 1 isoform b precursor"
                     /protein_id="NP_073560.2"
                     /db_xref="CCDS:CCDS49684.1"
                     /db_xref="GeneID:64706"
                     /db_xref="MGI:MGI:1890616"
                     /translation="MGAAAVRWHLYLLLALGARGRLVGGSGLPGAVDVDECSEGTDDC
                     HIDAICQNTPKSYKCLCKPGYKGEGRQCEDIDECENDYYNGGCVHDCINIPGNYRCTC
                     FDGFMLAHDGHNCLDVDECQDNNGGCQQICVNAMGSYECQCHSGFFLSDNQHTCIHRS
                     NEGMNCMNKDHGCAHICRETPKGGVACDCRPGFDLAQNQKDCTLTCNYGNGGCQHSCE
                     DTDTGPMCGCHQKYALHADGRTCIETCAVNNGGCDRTCKDTATGVRCSCPVGFTLQPD
                     GKTCKDINECLMNNGGCDHFCRNTVGSFECGCQKGHKLLTDERTCQDIDECSFERTCD
                     HICINSPGSFQCLCRRGYTLYGTTHCGDVDECSMNNGSCEQGCVNTKGSYECVCPPGR
                     RLHWNQKDCVEMNGCLSRSKASAQAQLSCGKVGGVENCFLSCLGHSLFMPDSESSYIL
                     SCGVPGLQGKTLPKRNGTSSSTGPGCSDAPTTPIRQKARFKIRDAKCHLQPRSQERAK
                     DTLRHPLLDNCHVTFVTLKCDSSKKRRRGRKSPSKEVSHITAEFEVEMKVDEASGTCE
                     ADCMRKRAEQSLQAAIKILRKSIGRNQFYVQVLGTEYEVAQRPAKALEGTGTCGIGQI
                     LQDGKCVPCAPGTYFSGDPGQCMPCVSGTYQDMEGQLSCTPCPSSEGLGLAGARNVSE
                     CGGQCSPGYFSADGFKPCQACPVGTYQPEPGRTGCFPCGGGLLTKHTGTASFQDCEAK
                     VHCSPGHHYNTTTHRCIRCPVGTYQPEFGQNHCISCPGNTSTDFDGSTNVTHCKNQHC
                     GGELGDYTGYIESPNYPGDYPANAECVWHIAPPPKRRILIVVPEIFLPIEDECGDVLV
                     MRKSASPTSVTTYETCQTYERPIAFTSRSRKLWIQFKSNEANSGKGFQVPYVTYDEDY
                     QQLIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESKEMFPRSF
                     IKLLRSKVSRFLRPYK"
     exon            222..353
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            354..482
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            483..617
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            618..743
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            744..860
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            861..977
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1100
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1217
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1340
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1460
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1565
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1682
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1820
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..2024
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2186
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2354
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2516
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2714
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2867
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2947
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..7842
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3903..3908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3928
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
     regulatory      7821..7826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7842
                     /gene="Scube1"
                     /gene_synonym="7330410C13Rik; A630023E24Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtcctgcgcg ccggctatgt ggctgggagc cggagcggta gcggagctcg ctgcagacag
       61 gagcgctcgc ggagcggtcg ctccgagcgt cccgtgcccc gcacgccagc acccggcgcg
      121 gcccgcagcg agcatgggcg cggcggccgt gcgctggcac ttgtacctac tgctcgctct
      181 gggagcgcgc gggcggctgg tggggggcag cgggctgcca ggagccgtgg acgtggatga
      241 atgctcagag ggcacagacg actgccacat agatgccatc tgccagaaca cacccaagtc
      301 ttacaaatgt ctctgcaagc ccggctataa gggggaagga aggcagtgtg aagacattga
      361 cgagtgtgag aatgactact acaacggggg ctgtgtccat gactgcatca acatccctgg
      421 gaactacagg tgcacctgct ttgatggctt catgctggcc catgatggac acaactgcct
      481 ggatgtagat gagtgtcaag acaacaacgg cggctgccag cagatctgcg tcaatgccat
      541 gggcagctat gaatgccagt gccacagcgg cttttttctc agtgacaacc aacacacctg
      601 catacaccgc tccaatgagg gtatgaactg catgaacaaa gaccatggct gtgcccacat
      661 ctgccgggag acgcccaaag gtggggtagc ctgtgactgc aggcccggct ttgaccttgc
      721 ccaaaaccag aaggactgca cactaacctg taactatgga aacggaggct gtcagcacag
      781 ctgcgaggac acggacacgg gccccatgtg tggctgccac cagaagtacg ccttacatgc
      841 agatggtcgg acgtgcatcg agacatgtgc agtgaacaac ggaggctgtg accggacgtg
      901 taaggacact gccactggcg tgcggtgcag ctgcccggtt ggattcacgc tgcagccaga
      961 tggaaagaca tgcaaagaca tcaacgagtg cttgatgaat aacggaggct gcgaccactt
     1021 ctgccgaaac accgtgggca gctttgaatg cggctgccaa aagggccaca agctactgac
     1081 ggacgagcgg acatgccaag acattgacga gtgttccttt gagcggacct gtgaccacat
     1141 ctgcatcaac tcccccggca gtttccagtg cctgtgccgc cgaggctaca cactctacgg
     1201 gacaacccac tgcggagacg tggatgaatg tagcatgaac aacggcagct gtgagcaggg
     1261 ctgtgtcaac accaagggca gctacgagtg tgtgtgccca ccagggagga ggctgcactg
     1321 gaatcagaag gactgtgtgg agatgaacgg gtgcctgtct cggtccaagg cctctgccca
     1381 ggcccagctg tcctgtggca aggtgggtgg agtggaaaac tgcttcctgt cctgcctggg
     1441 ccacagtctc ttcatgccag attcagaaag cagctacatc ctgagctgtg gtgttccagg
     1501 tctccagggc aagacactgc caaaacgcaa tggcactagc tccagtacag ggcccggctg
     1561 ctcagatgcc cccaccaccc ctatcagaca gaaggcccgc ttcaagatca gagatgccaa
     1621 gtgccatctc cagccccgca gccaggagcg agccaaggat accctgaggc atcccctttt
     1681 agacaactgc cacgtgactt ttgtgaccct caagtgtgac tcctccaaga agaggcgccg
     1741 aggccgcaag tccccatcca aggaggtgtc ccacatcaca gcagagtttg aggtggagat
     1801 gaaggtggat gaggcctcag gcacctgcga agcagactgc atgcggaagc gtgccgagca
     1861 gagcctgcaa gccgccatca agattctgcg caagtccatt ggtcggaacc agttctatgt
     1921 ccaggtcttg ggcactgagt acgaggtggc ccagcggcca gccaaagcac tggagggaac
     1981 cgggacttgc ggcataggcc aaatactaca ggacggcaag tgtgtaccgt gtgctcctgg
     2041 cacctacttc agtggtgacc caggccagtg tatgccatgc gtgtcgggaa cctaccagga
     2101 catggaaggc cagctcagct gcacgccatg ccccagcagc gaaggactgg gtctggctgg
     2161 tgcccgaaac gtatccgaat gtggaggcca gtgctctcca ggctacttct cagcggatgg
     2221 gttcaagcct tgccaggcct gccctgtggg cacataccag cccgagcctg ggcgaaccgg
     2281 ctgcttcccc tgtggaggcg ggctgctcac caaacacacg ggtaccgcct ctttccagga
     2341 ctgcgaggcc aaagtgcatt gctctcccgg acaccactac aataccacca cccaccggtg
     2401 tatccgctgt cccgtgggca cctatcagcc cgagtttggc cagaaccatt gcatctcctg
     2461 tccgggcaac accagcaccg attttgatgg ttccaccaac gtcacacact gcaagaacca
     2521 gcactgcggc ggcgaacttg gtgactacac aggctacatc gagtccccca actaccctgg
     2581 cgactaccca gccaatgcag agtgtgtgtg gcacatcgca cctcccccaa agcgcaggat
     2641 cctaatcgta gtcccggaga tcttcctacc catcgaggac gagtgtggtg atgtcctggt
     2701 catgagaaag agtgcctcgc ccacgtcagt caccacctat gagacgtgcc agacctatga
     2761 gcggcccatc gcctttacct cccgttcccg caaactctgg atccagttca aatccaacga
     2821 agccaacagt ggcaaagggt ttcaagtgcc ctatgtcacc tatgatgagg actaccagca
     2881 gctaatagaa gacatcgtgc gtgatgggcg cttatatgcc tcggagaacc atcaggagat
     2941 tttgaaagac aagaagctga tcaaggccct ctttgacgtg ctggcacacc cacaaaacta
     3001 cttcaaatac acagcccagg agtccaagga gatgttcccc cggtccttta tcaaacttct
     3061 gcgttccaaa gtgtctcggt tcctgcggcc ctacaaataa ccagccagct gtcctgtgtg
     3121 gggacagtgg gagccaagtg ggaggggaat gtgccttccc ttcacagcag gagccaaaag
     3181 gaggctccac gggcctccag gctgccgtga gaaacccatg acacatgcct ctggcaaagc
     3241 catccagcct gtactgcctc cgtggaaggc ctagacccag cagagacccc ttggtgggtc
     3301 tgccatgcct gtggggaaac actgcttcag aaagccttca cctaccctct gcctctattt
     3361 cccaggacac ctagaggatt cccttctgaa ccaccccagt ggggtctacc tgggccggga
     3421 cacactgtac ccaccatgtg gggagcggga gcaggactac tgtgcccttg gcctgacgtg
     3481 tggggagaga ggagcatggc cagttccctg caggctctgg aaagcatggg ccattgtcac
     3541 ccacacgggg ccaggccagg gctagagggg tgtgtggtag gtcgataaga ctgtgccagt
     3601 gcctgcggaa ccaacacacc ctgctgccag ctcctccagc cacaggcatg ggacactaag
     3661 ccgggtcatg gagctcaagg gactctgtga gagatgggac aggtcctcca ggagaggagt
     3721 ccacgtgctt gtgcttcgcc ttaatagaac atctaaagaa gttctatatc tatatatata
     3781 tataaatatt aaatatatac atttctctat gtgggcgggt attttagaag ggttctcttc
     3841 agcaactgta atactaacca gtgttgccaa cccctcccca ccccctaccc tctacccctg
     3901 tgattaaact gctcctctca gcgcttgaca cctctgtgtg agggagacca accttcgcac
     3961 ccatccgccc ccgcaggcct ggggagaaca cagatatgct tgcacaggct gtggagtgtg
     4021 acttgttgga gactggacta ggtgttgtgt ggaagtattg acaagggtgg gggtggggtg
     4081 ggggcttcac aagcaacatc actcccctca acagaacgca atggaaagaa agcatccccc
     4141 caccccctct gggttcgagc gttcaccgtc tccgcactgt gcctgcgggt caccagagac
     4201 aaggccttcc tcagggacgt acaggctgtc tgctgagcag cccccactct tctttatggc
     4261 aacaggagga gaaagcaagg cccaggagag gaggcctcca aggcagaagc tattgggagc
     4321 ctccctgatg ctcctcagcc tcttagagag gagcccttgt atggccaggt cacacggcat
     4381 cccagtggcc ggggagtcac cccctcacgc agagaccttg cctgtgcctc agatggcttg
     4441 ctgccagctg tcagatacac atagaagctt atatctctca aattaatgga tattagcact
     4501 tggaggaatg tagggggatc tcggaatgaa ctcctggggt tcctggtctc cagcccagcc
     4561 tgatggtgta aatgtactgt ttcagaaaac acatgaggct caccgaaggc tgaagatgtg
     4621 tgagccggca agccaggcct ggtgccacag cttgatgaga gggcaccaca catctgaaca
     4681 caggcttgga actcagggag aagctatggc taccacatgg gtccacaagg cagaagggat
     4741 caccggtggc agtcactcgc gttttgtgga gtgtggcagg tggacttgct gtgtctgatg
     4801 gaaggtcagg gtcaggatct actgcctctg cagttcacaa gccctgcagc ctggctgtac
     4861 ccgggagaag ctctcttccc cttccgttgg accacagggc atcctcggtc ctgcagaacg
     4921 accccagcaa cactcactgt ctccaggaaa acaaagcaaa caaggatacc tatggagaca
     4981 cgctaagaag gaattacgcg aatcggctca aaagttatgg tggccaagaa gtgatacagg
     5041 gtgtcatatg caaattagca aacaggaaag gcggcaatat tcaaaccaga gaccctgggc
     5101 atcggtgtag ccagtctgaa ggcctgagcc aggagcgctg tgtgggttgc ctgtctcagg
     5161 tcgttgtcat ttgccctccc tccaattttg cttcccaccc ccaccactct actgaaagaa
     5221 gagagcaaca gaggtgagga cctgctcttc tctagttcca gatcctgatg ctaattaatc
     5281 tcctccagga caccacagtc cttgccctac cacaccacaa gtatttgggg gctcagccct
     5341 gcccggttaa gttgacatat caagttaacc cactgaaggg cttcaggttt cattttttaa
     5401 tcttctcaaa aaccagaaaa aaaaaatcag aaaagtgttc tcaggttcac cttacgtcct
     5461 gcctacaact cccgagtggc ttttggtagt aactaccctg tgtacccatt aacaaagcca
     5521 actatgtcct ctctccagaa tgcccacccc tactgtggcc acatcatgcc cagtgtcccc
     5581 ccaggtagga gcagatacag tgcagaccag gcccctcagt cctcatggtc aagtcctttt
     5641 gttgctctca tcccctcccc ctgccccacc atgtgcccct ggctgccctt atccgcccaa
     5701 accaaaggct gagaggggga ggcctgtgtc cttccagctt cattgcacat ggtccccctc
     5761 ctgcaaccag gctacctgaa gacctttatg gaggtgaaag ggaaactaaa ttgttccctg
     5821 gattccagga gatggtcggc acagatggtc gtcacgtggg acctggaatt ggagacactg
     5881 cagcgagcag ctagaaagcc tcttgtgttc ctggcacctc ctggcactta tcttgggaat
     5941 gcctgcatga ggaaggagaa cgacctgccc tgagggtcag tgctcagccc agccctccac
     6001 tgttggtggc aaccgtggga cacctcattc cctaccccaa acgggccttt taattgtcct
     6061 ggtcacttgt actggttaca gagcctctca gcgatggcta caccaccctt ctcaccctgc
     6121 ctccatcccc aaatctcaag caacacaatc agcgggtact acctgtggcc cccaactcct
     6181 gtcacactgg ctattggtgg tgactacttt ctcaagccct ggctccccca agacagtctt
     6241 tcctaactgc ctccctactt ggagtagtca catactcaag tgcacaccac agcccgaggt
     6301 ggagacctgg gccacaccct tcccctcctg acatctttag tttggagcac acagaggaaa
     6361 tagaacctaa acacatgccc agaagtcctg gttcaaaagc tttactatgg agcctgcata
     6421 tgaagccacc tctggcttct acagaaggag gccctggctg ctccagcttg ggtggagcct
     6481 actgaaatgt ggaaactgtg tatcttatag agcctcctct cgtcctactc atccctcagc
     6541 agaaaaatgt gggcctaaaa tgagttgtgt cactcagcaa acatttcctg agtacctact
     6601 atataccaaa cttatagaaa gactaactag ggaacaacaa ggccacaaac aagccacctg
     6661 gcacagcagc cttctactca tccactgcat gacccggagg ctaggagagc ctgggcttcc
     6721 tccttgcggt ggggccagcc tgcaaagggc cactgacctg gcaagcattt acttctactt
     6781 caccaaagat gcccagagtg atgggtcagc ccttctgctc aagcaggccg tgcctggctg
     6841 gtgccattca gaccgaggcc gaaggacttg tgaacaaaag cacagagatg gctcagtggt
     6901 taggagcact gactcctttt ccgtaggtcc tgagttcaaa tcccagtaac cacatggtgg
     6961 ctgacaacca cccgtaatga gatctgatgc cctcttctgt tgctgtctga agacagctac
     7021 agtatactta catgtaataa ataaataaat aaataaataa ataaataaat aaataaatct
     7081 ttaggaaaaa atctgcaagg taccagatgg aggattcggg gtcaaggtga tgtggctggc
     7141 atagcctgtg ctctccccca gcaccttatt tagacagccc cccaacagta gtaggccctc
     7201 tgctggctgc agcctcccca gcctgcaccc agacatgcca gtgcacaggg cttggttgga
     7261 agctctgaga gaccaaataa catgcccaaa atgaagagtg aggagctgga gtagttaggg
     7321 ttgactttgg tggcagccca cctttcctaa cacacattca gagaaaggat agacttggca
     7381 tctcttgggt aggagtcagg gcccatccag accagtcacc atccagccat gcactgtcat
     7441 catcacccat aatgcctgca aaggcctagc ggtcctcagc cctgcacatg agagaggcac
     7501 agagtctgtc agcacaaaca ggaagcagct gggcttttaa gcagatctgt cagaaaccac
     7561 aagctatgcc aaggtcagag ccacgggcat ctagaaggca tccactctgc tcaagagacg
     7621 gatgcactcc tgcgccccgg tcttcggcct ggaccccaca cgttcatagt ctgtccagac
     7681 actgggtggc ggtcctaatg cacagtgacc aatatccaca taacctcatt tgccttccag
     7741 atgttcagat agtcaaagct tctctctacc ttgttttccc tcaatgcatg taattaaaca
     7801 cttgtggaaa tcgctttaac aataaacagg aaatgagcct aa
//
