LOCUS       NM_011324               3499 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Mus musculus sodium channel, nonvoltage-gated 1 alpha (Scnn1a),
            transcript variant 1, mRNA.
ACCESSION   NM_011324 XM_982260
VERSION     NM_011324.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Su,S., Wahl,A., Rugis,J., Suresh,V., Yule,D.I. and Sneyd,J.
  TITLE     A mathematical model of ENaC and Slc26a6 regulation by CFTR in
            salivary gland ducts
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 326 (5), G555-G566 (2024)
   PUBMED   38349781
  REMARK    GeneRIF: A mathematical model of ENaC and Slc26a6 regulation by
            CFTR in salivary gland ducts.
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Kim,H.K., Choi,S.H., Kim,D.H. and Jeong,Y.T.
  TITLE     Comprehensive mapping of Epithelial Na+ channel alpha expression in
            the mouse brain
  JOURNAL   Brain Struct Funct 229 (3), 681-694 (2024)
   PUBMED   38305875
  REMARK    GeneRIF: Comprehensive mapping of Epithelial Na[+] channel alpha
            expression in the mouse brain.
REFERENCE   3  (bases 1 to 3499)
  AUTHORS   Duan,X.P., Xiao,Y., Su,X.T., Zheng,J.Y., Gurley,S., Emathinger,J.,
            Yang,C.L., McCormick,J., Ellison,D.H., Lin,D.H. and Wang,W.H.
  TITLE     Role of Angiotensin II Type 1a Receptor (AT1aR) of Renal Tubules in
            Regulating Inwardly Rectifying Potassium Channels 4.2 (Kir4.2),
            Kir4.1, and Epithelial Na+ Channel (ENaC)
  JOURNAL   Hypertension 81 (1), 126-137 (2024)
   PUBMED   37909221
  REMARK    GeneRIF: Role of Angiotensin II Type 1a Receptor (AT1aR) of Renal
            Tubules in Regulating Inwardly Rectifying Potassium Channels 4.2
            (Kir4.2), Kir4.1, and Epithelial Na[+] Channel (ENaC).
REFERENCE   4  (bases 1 to 3499)
  AUTHORS   Ehret,E., Stroh,S., Auberson,M., Ino,F., Jager,Y., Maillard,M.,
            Szabo,R., Bugge,T.H., Frateschi,S. and Hummler,E.
  TITLE     Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and
            CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity
  JOURNAL   Cells 12 (19), 2342 (2023)
   PUBMED   37830556
  REMARK    GeneRIF: Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8
            and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3499)
  AUTHORS   Soares,A.G., Contreras,J., Mironova,E., Archer,C.R., Stockand,J.D.
            and Abd El-Aziz,T.M.
  TITLE     P2Y2 receptor decreases blood pressure by inhibiting ENaC
  JOURNAL   JCI Insight 8 (14), e167704 (2023)
   PUBMED   37279066
  REMARK    GeneRIF: P2Y2 receptor decreases blood pressure by inhibiting ENaC.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3499)
  AUTHORS   Hummler,E., Barker,P., Gatzy,J., Beermann,F., Verdumo,C.,
            Schmidt,A., Boucher,R. and Rossier,B.C.
  TITLE     Early death due to defective neonatal lung liquid clearance in
            alpha-ENaC-deficient mice
  JOURNAL   Nat Genet 12 (3), 325-328 (1996)
   PUBMED   8589728
REFERENCE   7  (bases 1 to 3499)
  AUTHORS   Chang,S.S., Grunder,S., Hanukoglu,A., Rosler,A., Mathew,P.M.,
            Hanukoglu,I., Schild,L., Lu,Y., Shimkets,R.A., Nelson-Williams,C.,
            Rossier,B.C. and Lifton,R.P.
  TITLE     Mutations in subunits of the epithelial sodium channel cause salt
            wasting with hyperkalaemic acidosis, pseudohypoaldosteronism type 1
  JOURNAL   Nat Genet 12 (3), 248-253 (1996)
   PUBMED   8589714
REFERENCE   8  (bases 1 to 3499)
  AUTHORS   Brooker,D.R., Kozak,C.A. and Kleyman,T.R.
  TITLE     Epithelial sodium channel genes Scnn1b and Scnn1g are closely
            linked on distal mouse chromosome 7
  JOURNAL   Genomics 29 (3), 784-786 (1995)
   PUBMED   8575777
REFERENCE   9  (bases 1 to 3499)
  AUTHORS   Meisler,M.H., Barrow,L.L., Canessa,C.M. and Rossier,B.C.
  TITLE     SCNN1, an epithelial cell sodium channel gene in the conserved
            linkage group on mouse chromosome 6 and human chromosome 12
  JOURNAL   Genomics 24 (1), 185-186 (1994)
   PUBMED   7896277
REFERENCE   10 (bases 1 to 3499)
  AUTHORS   Canessa,C.M., Schild,L., Buell,G., Thorens,B., Gautschi,I.,
            Horisberger,J.D. and Rossier,B.C.
  TITLE     Amiloride-sensitive epithelial Na+ channel is made of three
            homologous subunits
  JOURNAL   Nature 367 (6462), 463-467 (1994)
   PUBMED   8107805
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140324.3.
            
            On May 9, 2022 this sequence version replaced NM_011324.2.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus. The use of an alternative upstream start codon would
            result in a protein that is 1 aa longer.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140389.1, BC040814.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1035              AC140324.3         60791-61825         c
            1036-1309           AC140324.3         51981-52254         c
            1310-1500           AC140324.3         50882-51072         c
            1501-1604           AC140324.3         45265-45368         c
            1605-1768           AC140324.3         44766-44929         c
            1769-1867           AC140324.3         44230-44328         c
            1868-1985           AC140324.3         43942-44059         c
            1986-2064           AC140324.3         40485-40563         c
            2065-2122           AC140324.3         40320-40377         c
            2123-2178           AC140324.3         40089-40144         c
            2179-2254           AC140324.3         39868-39943         c
            2255-3499           AC140324.3         38222-39466         c
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.32 cM"
     gene            1..3499
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /note="sodium channel, nonvoltage-gated 1 alpha"
                     /db_xref="GeneID:20276"
                     /db_xref="MGI:MGI:101782"
     exon            1..1035
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             545..2644
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium channel, nonvoltage-gated, type I, alpha
                     polypeptide; amiloride-sensitive sodium channel subunit
                     alpha; amiloride-sensitive epithelial sodium channel;
                     epithelial sodium channel alpha subunit; alpha-ENaC;
                     alpha-NaCH; epithelial Na(+) channel subunit alpha;
                     nonvoltage-gated sodium channel 1 subunit alpha; ENaC
                     alpha; epithelial sodium channel subunit alpha"
                     /codon_start=1
                     /product="epithelial sodium channel subunit alpha isoform
                     1"
                     /protein_id="NP_035454.3"
                     /db_xref="CCDS:CCDS39641.2"
                     /db_xref="GeneID:20276"
                     /db_xref="MGI:MGI:101782"
                     /translation="MLDHTRAPELNLDLDLDVSNSPKGSMKGNNFKEQDLCPPLPMQG
                     LGKGDKREEQALGPEPSEPRQPTEEEEALIEFHRSYRELFQFFCNNTTIHGAIRLVCS
                     KHNRMKTAFWAVLWLCTFGMMYWQFALLFEEYFSYPVSLNINLNSDKLVFPAVTVCTL
                     NPYRYTEIKEDLEELDRITEQTLFDLYKYNSSYTRQAGGRRRSTRDLRGALPHPLQRL
                     RTPPPPNPARSARSASSSVRDNNPQVDRKDWKIGFQLCNQNKSDCFYQTYSSGVDAVR
                     EWYRFHYINILSRLPDTSPALEEEALGSFIFTCRFNQAPCNQANYSQFHHPMYGNCYT
                     FNNKNNSNLWMSSMPGVNNGLSLTLRTEQNDFIPLLSTVTGARVMVHGQDEPAFMDDG
                     GFNVRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPSKYTQQVCIHSCFQE
                     NMIKKCGCAYIFYPKPKGVEFCDYLKQSSWGYCYYKLQAAFSLDSLGCFSKCRKPCSV
                     TNYKLSAGYSRWPSVKSQDWIFEMLSLQNNYTINNKRNGVAKLNIFFKELNYKTNSES
                     PSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIFDLLVITLIMLLHRFRSRYWSPGR
                     GARGAREVASTPASSFPSRFCPHPTSPPPSLPQQGTTPPLALTAPPPAYATLGPSASP
                     LDSAVPGSSACAPAMAL"
     exon            1036..1309
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1500
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1604
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1768
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1867
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1985
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2064
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2122
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2178
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2254
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..3499
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3478..3483
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /note="hexamer: AATAAA"
     polyA_site      3499
                     /gene="Scnn1a"
                     /gene_synonym="ENaC; mENaC; SCNEA; Scnn1"
                     /note="major polyA site"
ORIGIN      
        1 agtcacagcc cagccacacc tggagccggg agcaggaggc agctccggcc tcctgcaacc
       61 cacggtcccc gaggcagaga aggaggtagc agggagctgg aggccagggc tagagagcct
      121 agagaagagg acccaggagg agatagggaa ggcaggaaag gaagtgaggc aggatcagag
      181 agcctggcac agagagggag acccaaagag aagcgggagt cagctgggcc aagagggcgt
      241 gaaagccgga gagtcaaaca gtccgggagg aaaaaggggc aagagggaga ggcgctaagc
      301 cagacagggt gcctgctgtg gagacccagg gaggcgctag cgggcaaacg aaggtggcct
      361 tcgctgtgac cacttcgctc tgtggccact ccagtgaagc tccgtgctgc ctggttggcc
      421 ccaactccag aaggtcagct ggctcctgga gaggtggagg agggtgggag gactgaggaa
      481 gagggaactc agcgtgggat gcgggcaccg tcacggacgg ccccattctg cctccatact
      541 aatgatgctg gaccacacca gagcccctga gctcaacctt gacctagacc ttgacgtctc
      601 caactcaccg aagggatcca tgaagggcaa caatttcaag gagcaagacc tttgtcctcc
      661 tctgcccatg caaggactgg gcaaggggga caagcgtgaa gaacaggcgc tgggcccgga
      721 accctcagag ccccggcagc ccaccgagga ggaggaggca ctgatcgagt tccaccgctc
      781 ctaccgggag ctcttccagt tcttctgcaa caataccacc atccacggtg ccatccgcct
      841 ggtgtgctcc aagcacaacc gcatgaagac ggccttctgg gcggtgctct ggctctgcac
      901 cttcggcatg atgtactggc agtttgcttt gctgttcgag gaatacttca gctaccccgt
      961 gagtctcaac atcaacctca attcggacaa gctggtcttc cctgccgtca ctgtgtgcac
     1021 ccttaatcct tacagataca ctgaaattaa agaggatctg gaagagctgg accgcatcac
     1081 ggaacagacg ctttttgacc tgtacaaata caactcttcc tacactcgcc aggctggggg
     1141 ccgccgccgc agcacccgcg acctccgggg tgctctccca caccccctgc agcgcctgcg
     1201 cacaccacct ccgcccaatc ccgcccgctc ggcgcgcagc gcgtcctcca gtgtacgcga
     1261 caacaatccc caagtggaca ggaaggactg gaaaatcggc ttccaactgt gcaaccagaa
     1321 caaatcagac tgcttctacc agacatactc atccggggtg gatgccgtga gagaatggta
     1381 ccgcttccat tacatcaaca ttctgtccag actgcccgac acctcgcctg ctctagagga
     1441 agaagccctg ggcagcttca tctttacctg tcgtttcaac caggccccct gcaatcaggc
     1501 gaattattct cagttccacc accccatgta tgggaactgc tacactttca acaacaagaa
     1561 caactccaat ctctggatgt cttccatgcc tggagtcaac aatggtttgt ccctgacact
     1621 gcgcacagag cagaatgact tcatccccct gctgtccaca gtgacggggg ccagggtgat
     1681 ggtgcacggt caggatgagc ctgcttttat ggatgatggt ggcttcaacg tgaggcctgg
     1741 tgtggagacc tccatcagta tgagaaagga agccctggac agcctcggag gcaactacgg
     1801 agactgcact gagaatggca gtgatgtccc tgtcaagaac ctttacccct ccaagtacac
     1861 acagcaggtg tgcattcact cctgcttcca ggagaacatg atcaagaagt gtggctgtgc
     1921 ctacatcttc taccctaagc ccaagggtgt agagttctgt gactacctaa agcagagctc
     1981 ctggggctac tgctactata aactgcaggc tgccttctcc ttggatagcc tgggctgctt
     2041 ctccaagtgc aggaagccgt gcagtgtgac caactacaag ctctctgctg gctactcaag
     2101 atggccgtct gtgaagtccc aggattggat cttcgagatg ctatccttgc agaataatta
     2161 cacgatcaac aacaaaagaa acggagttgc taaactcaac atcttcttca aagagctgaa
     2221 ctataaaact aattcggagt ctccctctgt cacgatggtc agcctcctgt ccaacctggg
     2281 cagccagtgg agcctgtggt tcggctcatc tgtgctgtcc gtggtggaaa tggcggagct
     2341 catcttcgac ctcctggtca tcacactcat catgttactg cacaggttcc ggagccggta
     2401 ctggtctcca ggacgagggg ccaggggtgc cagggaggtg gcctctaccc cagcttcctc
     2461 cttcccttcc cgtttctgtc cccaccctac atccccgcca ccttctttgc cccagcaggg
     2521 cacgacccct cccctggccc tgacagcccc tccacctgcc tatgctaccc taggcccctc
     2581 tgcctctcca ctggactcgg ctgtgcctgg ctcttctgcc tgtgctccgg ccatggcact
     2641 ctgagagagg agagtgctcc tctcacccag gccagtgctc ctgtcacttc agcacatctt
     2701 ccacagctgc ccagctgtct ttggtgtgtc ccggaggaac aggctaagca aggggcccag
     2761 gaagttgtcc agaggacagg ggctaatgag ctgctcagag ctgccctgcc cctgcttctg
     2821 aacactgctt tccacacaag cacgggcaag tcccctttac ccttggatca gccaagccag
     2881 acttggagct ttgacaagga acgttcccgg gaaacgacca aacgaaccga acacatataa
     2941 acaaggcaca gagaagtggc cacagccttc ccaccccacg accagagact ggcctggcct
     3001 cactgctttc aaggacacag atgtctgcta cccctcttga acttgggtgg ggaaccccac
     3061 ccaaaagccc ccttgttagc tctttggcaa ttctccttcc ctcactcctc agggtggggg
     3121 ctagagtaag cctgacatcc tcctccattc tcaagactct ctctctttca tttggtaccc
     3181 tgtaccccag tgcctctgcg tcgcctcctt cttgtgtgcc ttctgagctg tttcttcagc
     3241 ctagaaactc cctgctcaaa ggcacctttg cttttgtgaa ctcgttcacc ctatcctgtc
     3301 tcccccagga ttgcccccct ctcccctcac ccccacagca tgctgtatta gatgctcaca
     3361 ttcttttgtg tccatctccc tgggtagact gaactgtgct cagggatgag ctttgctcat
     3421 ttttgtatcc ttccgttcta gcccagtatc ccacttggac caggtaggca gatactcaat
     3481 aaatgcttgt tccatcaaa
//
