LOCUS       NM_001290674            9865 bp    mRNA    linear   ROD 12-DEC-2024
DEFINITION  Mus musculus sodium channel, voltage-gated, type IX, alpha (Scn9a),
            transcript variant 1, mRNA.
ACCESSION   NM_001290674 XM_006499032
VERSION     NM_001290674.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Bangash,M.A., Cubuk,C., Iseppon,F., Haroun,R., Garcia,C.,
            Luiz,A.P., Arcangeletti,M., Gossage,S.J., Santana-Varela,S.,
            Cox,J.J., Lewis,M.J., Wood,J.N. and Zhao,J.
  TITLE     Analgesic targets identified in mouse sensory neuron somata and
            terminal pain translatomes
  JOURNAL   Cell Rep 43 (8), 114614 (2024)
   PUBMED   39163201
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Fu,W., Vasylyev,D., Bi,Y., Zhang,M., Sun,G., Khleborodova,A.,
            Huang,G., Zhao,L., Zhou,R., Li,Y., Liu,S., Cai,X., He,W., Cui,M.,
            Zhao,X., Hettinghouse,A., Good,J., Kim,E., Strauss,E., Leucht,P.,
            Schwarzkopf,R., Guo,E.X., Samuels,J., Hu,W., Attur,M., Waxman,S.G.
            and Liu,C.J.
  TITLE     Nav1.7 as a chondrocyte regulator and therapeutic target for
            osteoarthritis
  JOURNAL   Nature 625 (7995), 557-565 (2024)
   PUBMED   38172636
REFERENCE   3  (bases 1 to 9865)
  AUTHORS   Deng,L., Dourado,M., Reese,R.M., Huang,K., Shields,S.D.,
            Stark,K.L., Maksymetz,J., Lin,H., Kaminker,J.S., Jung,M.,
            Foreman,O., Tao,J., Ngu,H., Joseph,V., Roose-Girma,M., Tam,L.,
            Lardell,S., Orrhult,L.S., Karila,P., Allard,J. and Hackos,D.H.
  TITLE     Nav1.7 is essential for nociceptor action potentials in the mouse
            in a manner independent of endogenous opioids
  JOURNAL   Neuron 111 (17), 2642-2659 (2023)
   PUBMED   37352856
  REMARK    GeneRIF: Nav1.7 is essential for nociceptor action potentials in
            the mouse in a manner independent of endogenous opioids.
REFERENCE   4  (bases 1 to 9865)
  AUTHORS   Wimalasena,N.K., Taub,D.G., Shim,J., Hakim,S., Kawaguchi,R.,
            Chen,L., El-Rifai,M., Geschwind,D.H., Dib-Hajj,S.D., Waxman,S.G.
            and Woolf,C.J.
  TITLE     Nav1.7 gain-of-function mutation I228M triggers age-dependent
            nociceptive insensitivity and C-LTMR dysregulation
  JOURNAL   Exp Neurol 364, 114393 (2023)
   PUBMED   37003485
  REMARK    GeneRIF: Nav1.7 gain-of-function mutation I228M triggers
            age-dependent nociceptive insensitivity and C-LTMR dysregulation.
REFERENCE   5  (bases 1 to 9865)
  AUTHORS   Jami,S., Deuis,J.R., Klasfauseweh,T., Cheng,X., Kurdyukov,S.,
            Chung,F., Okorokov,A.L., Li,S., Zhang,J., Cristofori-Armstrong,B.,
            Israel,M.R., Ju,R.J., Robinson,S.D., Zhao,P., Ragnarsson,L.,
            Andersson,A., Tran,P., Schendel,V., McMahon,K.L., Tran,H.N.T.,
            Chin,Y.K., Zhu,Y., Liu,J., Crawford,T., Purushothamvasan,S.,
            Habib,A.M., Andersson,D.A., Rash,L.D., Wood,J.N., Zhao,J.,
            Stehbens,S.J., Mobli,M., Leffler,A., Jiang,D., Cox,J.J.,
            Waxman,S.G., Dib-Hajj,S.D., Neely,G.G., Durek,T. and Vetter,I.
  TITLE     Pain-causing stinging nettle toxins target TMEM233 to modulate
            NaV1.7 function
  JOURNAL   Nat Commun 14 (1), 2442 (2023)
   PUBMED   37117223
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9865)
  AUTHORS   Fotia,A.B., Ekberg,J., Adams,D.J., Cook,D.I., Poronnik,P. and
            Kumar,S.
  TITLE     Regulation of neuronal voltage-gated sodium channels by the
            ubiquitin-protein ligases Nedd4 and Nedd4-2
  JOURNAL   J Biol Chem 279 (28), 28930-28935 (2004)
   PUBMED   15123669
REFERENCE   7  (bases 1 to 9865)
  AUTHORS   Castillo,S.O., Xiao,Q., Lyu,M.S., Kozak,C.A. and Nikodem,V.M.
  TITLE     Organization, sequence, chromosomal localization, and promoter
            identification of the mouse orphan nuclear receptor Nurr1 gene
  JOURNAL   Genomics 41 (2), 250-257 (1997)
   PUBMED   9143501
REFERENCE   8  (bases 1 to 9865)
  AUTHORS   Kozak,C.A. and Sangameswaran,L.
  TITLE     Genetic mapping of the peripheral sodium channel genes, Scn9a and
            Scn10a, in the mouse
  JOURNAL   Mamm Genome 7 (10), 787-788 (1996)
   PUBMED   8854872
REFERENCE   9  (bases 1 to 9865)
  AUTHORS   Beckers,M.C., Ernst,E., Belcher,S., Howe,J., Levenson,R. and
            Gros,P.
  TITLE     A new sodium channel alpha-subunit gene (Scn9a) from Schwann cells
            maps to the Scn1a, Scn2a, Scn3a cluster of mouse chromosome 2
  JOURNAL   Genomics 36 (1), 202-205 (1996)
   PUBMED   8812438
REFERENCE   10 (bases 1 to 9865)
  AUTHORS   D'Arcangelo,G., Paradiso,K., Shepherd,D., Brehm,P., Halegoua,S. and
            Mandel,G.
  TITLE     Neuronal growth factor regulation of two different sodium channel
            types through distinct signal transduction pathways
  JOURNAL   J Cell Biol 122 (4), 915-921 (1993)
   PUBMED   8394370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK141871.1, AL928623.5, AL928726.5 and BP765553.1.
            
            On Mar 15, 2014 this sequence version replaced XM_006499032.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC172147.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849375,
                                           SAMN00849380 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-815               AK141871.1         2-816
            816-934             AL928623.5         39836-39954         c
            935-1024            AL928623.5         39443-39532         c
            1025-1153           AL928623.5         38768-38896         c
            1154-1245           AL928623.5         37205-37296         c
            1246-1458           AL928623.5         36011-36223         c
            1459-1522           AL928623.5         24005-24068         c
            1523-1664           AL928623.5         22645-22786         c
            1665-1871           AL928623.5         20688-20894         c
            1872-2156           AL928623.5         16700-16984         c
            2157-2528           AL928623.5         13929-14300         c
            2529-2658           AL928623.5         10935-11064         c
            2659-2897           AL928623.5         9763-10001          c
            2898-3071           AL928623.5         7779-7952           c
            3072-3428           AL928623.5         6727-7083           c
            3429-3905           AL928726.5         118788-119264       c
            3906-4023           AL928726.5         107495-107612       c
            4024-4178           AL928726.5         100700-100854       c
            4179-4352           AL928726.5         97029-97202         c
            4353-4475           AL928726.5         93793-93915         c
            4476-4757           AL928726.5         86789-87070         c
            4758-4811           AL928726.5         86066-86119         c
            4812-4949           AL928726.5         85259-85396         c
            4950-5054           AL928726.5         80162-80266         c
            5055-5325           AL928726.5         79555-79825         c
            5326-9158           AL928726.5         72952-76784         c
            9159-9865           BP765553.1         1-707               c
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 39.13 cM"
     gene            1..9865
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /note="sodium channel, voltage-gated, type IX, alpha"
                     /db_xref="GeneID:20274"
                     /db_xref="MGI:MGI:107636"
     exon            1..507
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            508..815
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             558..6512
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /note="isoform a is encoded by transcript variant 1;
                     sodium channel, voltage-gated, type IX, alpha polypeptide;
                     sodium channel 25; sodium channel protein type 9 subunit
                     alpha; voltage-gated sodium channel alpha subunit Nav1.7;
                     peripheral sodium channel 1"
                     /codon_start=1
                     /product="sodium channel protein type 9 subunit alpha
                     isoform a"
                     /protein_id="NP_001277603.1"
                     /db_xref="CCDS:CCDS71063.1"
                     /db_xref="GeneID:20274"
                     /db_xref="MGI:MGI:107636"
                     /translation="MAMLPPPGPQSFVHFTKQSLALIEQRISEEKAKGHKDEKKDDEE
                     EGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNA
                     TPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMSNPPDWTKNVEYTFT
                     GIYTFESLIKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRV
                     LRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCF
                     RKDLEQNETLESIMSTAESEEELKRYFYYLEGSKDALLCGFSTDSGQCPEGYECVTAG
                     RNPDYGYTSFDTFGWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFY
                     LINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSL
                     GRSRIMGLSESSSETSRLSSKSAKERRNRRKKKKQKLSSGEEKGDDEKLSKSGSEESI
                     RKKSFHLGVEGHHRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDLGSE
                     TEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCN
                     GVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQMRKKRLSSSYFLSEDMLNDP
                     HLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIV
                     MDPFVDLAITICIVLNTLFMAMEHHPMTDEFKNVLAVGNLVFTGIFAAEMVLKLIAMD
                     PYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIK
                     IIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINENCKLPRWHMNDFFH
                     SFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVYMMVMVIGNLVVLNLFLALLLSSFSS
                     DNLTAIEEDTDANNLQIAVARIKRGINYVKQTLREFILKSFSKKPKGSKDTKRTADPN
                     NKRENYISNRTLAEISKDHNFLKEKDKISGFSSSLDKSFMDENDYQSFIHNPSLTVTV
                     PIAPGESDLENMNTEELSSDSDSDYSKERRNRSSSSECSTVDNPLPGEEEAEAEPINA
                     DEPEACFTDGCVRRFPCCQVNIDSGKGKVWWTIRKTCYRIVEHSWFESFIVLMILLSS
                     GALAFEDIYIEKKKTIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFL
                     IVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIM
                     NVLLVCLIFWLIFSIMGVNLFAGKFYECVNTTDGSRFSVSQVANRSECFALMNVSGNV
                     RWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNAQPIYEYNLYMYIYFV
                     IFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKP
                     IPRPGNKFQGCIFDLVTNQAFDITIMVLICLNMVTMMVEKEGQTDYMSFVLYWINVVF
                     IILFTGECVLKLISLRHYYFTVGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVI
                     RLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVK
                     KEAGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPKKVHPGSSVEG
                     DCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVW
                     EKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFA
                     FTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATIIQRAYRR
                     YRLRQNVKNISSIYIKDGDRDDDLPNKEDIVFDNVNENSSPEKTDATASTISPPSYDS
                     VTKPDQEKYETDKTEKEDKEKDESRK"
     exon            816..934
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1024
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1153
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1245
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1458
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1522
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1664
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1871
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2156
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2528
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2658
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2897
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..3071
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            3072..3428
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3905
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            3906..4023
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4024..4178
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4179..4352
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4353..4475
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4476..4757
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4758..4811
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4812..4949
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            4950..5054
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            5055..5325
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     exon            5326..9850
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9829..9834
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /note="hexamer: AATAAA"
     polyA_site      9850
                     /gene="Scn9a"
                     /gene_synonym="mKIAA4197; Nav1.7; PN1"
                     /note="major polyA site"
ORIGIN      
        1 gcacacaccc cacccagaga ggtggccgcg ggtgagaggc tgtgcgaccc acctcctgtc
       61 ccttcttcta gcacggagta tagaggctgc cgcatcccta gcatcaccac gcccaatcgc
      121 caggtctccg ccctgcaccg tctaccgggc tgggaggagg gagctagggg tggggacccg
      181 agagctcagg gagcatcgag gaggcctagc gacgcggttg gcagccgagg gcggggtcgc
      241 ttcatcctga gcagactaga aacagacaga ctccgtgcag gcctagcctg cgctccaatt
      301 gcgactctag ccttttcaat cctgcccact gtgcaggctg gtctggtcta gcctgggtat
      361 cccggactcc tagtagcagg cactgtgagc aacaggattt caaagaaaga agcagaggga
      421 agaaagaagc ctgggaagag aggaagactt cccttggatc agaatccgca ggtgcactca
      481 ccgggtgggc atgatccgtg gggccaggcc tcttaggtaa ggatccgaag gggaaataaa
      541 acctacagga tgagaagatg gcgatgttgc ctccgccagg acctcagagc ttcgttcact
      601 tcacaaaaca gtcccttgcc ctcattgaac aacgcatttc tgaagaaaaa gccaagggac
      661 acaaagatga aaagaaagat gatgaggaag aaggtcccaa gcccagtagt gacttggaag
      721 caggtaaaca gctacccttc atctacggag acattccccc gggaatggtg tcagagcccc
      781 tggaggacct ggacccatac tatgcagaca aaaaaacttt tatagtattg aacaaaggga
      841 aagcaatctt ccgtttcaac gccacccctg ctttgtacat gctgtctccc ttcagtcctc
      901 tcagaagaat atctattaag attttagtgc actccttatt cagcatgcta atcatgtgca
      961 caattctgac aaactgcata ttcatgacca tgagcaaccc tccagattgg accaaaaacg
     1021 tagagtacac ttttactggg atatatactt ttgaatcact cataaaaatc cttgcaagag
     1081 gcttttgcgt gggcgaattc accttcctcc gtgacccttg gaactggctg gactttgttg
     1141 tcattgtttt tgcgtattta acagaatttg taaacctagg caatgtttca gctcttcgaa
     1201 ctttcagagt cttgagagct ttgaaaacta tttctgtaat tccaggacta aaaaccatcg
     1261 tgggggccct gatccaatca gtgaagaagc tctctgacgt catgatcctc actgtgttct
     1321 gtctcagtgt gttcgcacta attggactac aactgtttat gggcaacttg aagcataaat
     1381 gtttccggaa ggaccttgag cagaatgaaa cattagaaag catcatgagt actgctgaga
     1441 gtgaagaaga attgaaaaga tatttttatt acttggaggg atccaaagat gctcttcttt
     1501 gcggtttcag cacagattca gggcagtgtc ctgaagggta cgagtgtgtg acagctggca
     1561 gaaacccaga ttatggctac acaagctttg acacgttcgg ctgggccttc ttggccttgt
     1621 ttcggctaat gactcaggac tactgggaga acctttatca acagacactg cgtgctgctg
     1681 gcaaaaccta catgattttc tttgtcgtgg tgatatttct gggatccttt tacctgataa
     1741 acttgatcct ggctgtggta gccatggcgt acgaggaaca gaaccaggcc aacatcgaag
     1801 aagctaaaca gaaagagtta gaatttcagc agatgttaga ccgactcaaa aaagagcagg
     1861 aagaagccga ggcgatcgct gcagccgctg ctgagtacac gagtttaggg cggagcagga
     1921 ttatgggact ctctgagagc tcttcagaaa cctccaggct gagctcaaag agtgccaagg
     1981 agagaagaaa ccgaagaaag aaaaaaaaac agaagctgtc cagtggcgag gaaaagggtg
     2041 atgatgagaa gctgtccaag tcagggtcag aggaaagcat ccgaaagaaa agcttccatc
     2101 tcggcgtgga agggcaccac cgggccaggg aaaagaggct gtccaccccc aaccagtcac
     2161 cactcagcat tcgtgggtcc ttgttttctg ccaggcgcag cagcagaaca agtctcttca
     2221 gttttaaggg gcgaggaaga gatctgggat ctgaaacgga atttgctgat gatgagcata
     2281 gcatttttgg agacaacgag agcagaaggg gttcactatt tgtaccccat agaccccggg
     2341 agcggcgcag cagtaacatc agccaggcca gtaggtcccc accagtgctg ccggtgaacg
     2401 ggaagatgca cagtgcagtg gactgcaatg gcgtggtgtc gcttgttgat ggaccctcag
     2461 ccctcatgct ccccaatgga cagcttcttc cagaggtgat aatagataag gcaacttccg
     2521 acgacagcgg cacaactaat cagatgcgta aaaaaaggct ctctagttct tactttttgt
     2581 ctgaggacat gctgaatgac ccacatctca ggcaaagggc catgagcaga gcaagcattc
     2641 taaccaacac agtagaagaa cttgaagaat ctagacaaaa atgtccacca tggtggtaca
     2701 gatttgctca cacattttta atctggaatt gttctccata ttggataaaa ttcaaaaagt
     2761 tcatctattt tattgtaatg gatccttttg tagatcttgc aattaccatt tgcatagttt
     2821 taaacacctt gtttatggct atggagcacc atccaatgac ggatgaattc aaaaatgtac
     2881 ttgcagtcgg gaacctggtc ttcacaggga tcttcgcagc tgaaatggta ctgaagttaa
     2941 tagccatgga tccctatgaa tatttccaag tagggtggaa tatttttgac agcctgattg
     3001 tgacgttgag tttggtggag cttttcctag cagatgtgga aggattatca gtcctgcggt
     3061 cctttagatt gctgcgagtc ttcaagttgg caaaatcctg gcccacactg aatatgctca
     3121 ttaagatcat cggcaactcg gtgggcgcac tgggcaacct gaccctggtg ctggccatca
     3181 tcgtcttcat ttttgccgtg gtcggcatgc agctgtttgg aaagagctac aaggagtgtg
     3241 tttgcaagat caacgagaac tgcaagctcc cacgctggca catgaacgac ttcttccact
     3301 ccttcctgat cgtgttccgt gtgctgtgtg gggagtggat agagaccatg tgggactgca
     3361 tggaggttgc gggccagacc atgtgcctta ttgtttacat gatggtcatg gtgattggga
     3421 accttgtggt cctgaacctg tttctggctt tattactgag ttcctttagt tctgacaatc
     3481 ttacagcaat tgaagaagac accgacgcaa acaacctcca gattgcagtg gccagaatta
     3541 aaagagggat caattatgtg aaacagaccc tgcgtgaatt cattctaaag tcattttcca
     3601 aaaagccaaa gggctccaag gacacaaaac gaacagcgga tcccaacaac aaaagagaaa
     3661 actatatctc aaaccgtacc cttgcggaga taagcaaaga tcacaatttc ctcaaagaaa
     3721 aggataagat cagtggtttt agcagcagtc tagacaaaag ctttatggat gaaaacgatt
     3781 accagtcctt tattcataat cccagcctca cagtgacagt gcccattgca cctggggagt
     3841 ctgatttgga gaatatgaac acagaagagc ttagcagtga ctcagatagt gactacagca
     3901 aagagagacg gaaccgatca agttcttcag agtgcagcac agttgataac cctctgccag
     3961 gagaagagga ggcagaagct gagcctatca atgcagatga gcccgaagcc tgttttacag
     4021 atggctgtgt gaggagattt ccatgctgcc aagttaacat agactccggg aaagggaaag
     4081 tttggtggac catccggaag acctgctaca ggatagtgga gcacagctgg tttgaaagct
     4141 tcattgttct catgatcctg ctcagcagtg gagctctggc ttttgaggat atatatattg
     4201 aaaagaaaaa gaccattaag attatcctgg agtatgctga caagatattc acctacatct
     4261 tcattctgga aatgcttcta aaatgggtgg catacgggta taaaacatat ttcactaatg
     4321 cctggtgttg gctggacttc ttaattgttg atgtgtctct agttacttta gtagccaaca
     4381 ctcttggcta ctcagacctt ggccccatta aatctctacg gacactgagg gccctaagac
     4441 ccctaagagc tttgtctaga tttgaaggaa tgagggtagt ggtcaacgca ctcataggag
     4501 caatcccttc catcatgaat gtgcttcttg tgtgccttat attctggcta atatttagca
     4561 tcatgggagt caatctgttt gctggcaagt tctatgagtg tgttaacacc acagatggct
     4621 cacgattttc tgtatctcaa gttgcaaacc gttctgagtg ttttgccctg atgaatgtta
     4681 gtggaaatgt gcgatggaaa aacctgaaag taaacttcga taacgttgga cttggttacc
     4741 tgtcgctgct tcaagttgca acgttcaagg gctggatgga tattatgtat gcagcagttg
     4801 actctgttaa tgtaaatgca caaccaatat atgaatacaa cctctacatg tacatttatt
     4861 ttgtcatctt catcatcttt ggctcattct tcactttgaa cttgttcatt ggtgtcatca
     4921 tcgataattt caaccaacag aagaagaagc ttggaggtca agatatcttt atgacagaag
     4981 aacagaagaa atactataat gcaatgaaga agctggggtc caagaaacca caaaaaccaa
     5041 ttccgaggcc agggaacaaa ttccaaggat gcatatttga cttagtgaca aaccaagctt
     5101 ttgatatcac catcatggtt cttatctgcc tcaatatggt aaccatgatg gtagaaaaag
     5161 aggggcaaac tgactacatg agttttgtgc tatactggat caacgtggtc ttcatcatcc
     5221 tgttcactgg ggagtgtgtg ctgaagctga tctctctcag gcattactac ttcactgtgg
     5281 gatggaacat ttttgatttt gtggtagtga tcctctccat tgtaggaatg tttctcgctg
     5341 agatgataga gaagtatttc gtgtctccta ccctgttccg agtcattcgc ctggccagga
     5401 ttggacgaat cctacgcctg atcaaaggcg ccaaggggat ccgcacgctg ctctttgctc
     5461 tgatgatgtc ccttcctgcg ctgttcaaca tcggcctcct gcttttcctc gtcatgttca
     5521 tctacgccat ctttgggatg tccaactttg cctacgttaa aaaggaagct ggaattaatg
     5581 acatgttcaa ctttgagacc ttcggcaaca gcatgatctg cctgttccaa atcaccacct
     5641 ctgcgggctg ggatggactg ctggccccca tcctcaacag tgcacctcct gactgtgacc
     5701 caaaaaaggt tcacccagga agttcagtgg aaggggactg tggaaatcca tctgtgggaa
     5761 ttttctactt tgtcagctac atcatcatat ccttcctggt tgtggtgaac atgtacattg
     5821 ctgtcatcct ggagaacttc agcgttgcca cagaagaaag tactgagccc ctgagtgagg
     5881 acgactttga gatgttctac gaagtctggg agaagttcga ccctgacgcc acccagttca
     5941 tagagttctg caagctctct gactttgcag cagccctgga tcctcccctc ctcatcgcaa
     6001 agccaaacaa agtccagctc attgccatgg acctgcccat ggtgagtgga gaccgcatcc
     6061 actgcctgga catcttattt gcttttacaa agcgggtcct gggcgagagc ggagagatgg
     6121 attcccttcg ttcacagatg gaagaaaggt ttatgtcagc caatccttct aaagtgtcct
     6181 atgagcccat cacaaccaca ctgaagcgaa aacaagagga tgtatctgcg actatcattc
     6241 agcgtgctta cagacggtac cgccttaggc aaaacgtcaa gaatatatca agtatatata
     6301 taaaagatgg agacagagat gacgatttgc ccaataaaga agatatagtt tttgataatg
     6361 ttaacgagaa ctcaagtcca gaaaagacag atgcaacagc ctctaccatc tctccacctt
     6421 cctatgacag tgtcacaaag ccagatcaag agaaatatga aacagacaaa acggagaagg
     6481 aagacaaaga gaaagacgaa agcaggaaat agagcttcgg ttttgataca ctgtttacag
     6541 cctgcgaagg tgactcactc gtgttaataa gactctttta cggaggtcta tgccaaactc
     6601 tttttatcaa atattctcaa aggcagcaca gccactagct ctgatccagt gaaacaagag
     6661 agaagcattt acacatggct actttttgcg ttggtcaatg attctttaag aattgtgcat
     6721 gtaactctac agggaataat cattattgca atcaagggtg acttaatgat tttaaatatc
     6781 agaaaaccac atagaacatt ttctcttttg cctccatttc tttccctaga ttctaagtag
     6841 atgtgtaccc atgtgaatat agaaattcag gcgcacatgc tcacagtcac aaacacaaac
     6901 aggattagct gtgatttgga attcgatgta aatatttcac ctgtgatttg caatgaaatt
     6961 ccttgtaaaa gaaatgcgaa ttagtgatga aggttttgtg aaaacatctt atcattaggg
     7021 agtcagaatt tctgtccata aagaattcag tttatatttt gaggtgctga aacttatcct
     7081 acattgcatc aaaatcaatt tataggtatc tgtaaaatgt catgggactg aaaaacatat
     7141 ataggctact tgtttaagaa atggctttca ttcatataga taggcattca ccttgattta
     7201 tggacatctt tggcattttg tgatcacatg attcttccac aaaattgctt agctggaact
     7261 tcaggcacac atcacagaga acagctaccc agtcttatgc ccctctctgt ttgtacaata
     7321 atcacagagc ttgaaacatt atttgaacta taaatatcag gtttctccac atagacatat
     7381 gaatattgtt aacagaaaaa aattttattt acagcgtttt atttactaat atttatccaa
     7441 tctagtttgc ccaatgagac agctcatgac tcacatctga aagccagttg ccacatttat
     7501 cttcttatgt aactttggtt tgtcatactt tatgtctaag caaattgaat gtctcctttc
     7561 taatgagatg taccctgaaa tgcagttagg tacttgatac tttagcgctt gtttgagcag
     7621 atgactggag cacagtgtgg actgcatctc ttaaatacaa tccttaatgt gtttggcagc
     7681 ttctcaggtt acaaggaaca cccggctttt agtgtctatc tgttcaccag gtgtttagta
     7741 tgaatgaaac ggcattcaaa gagtgagtct cactggcttg ctttattact gatgccttcc
     7801 ctatggagaa ttaatcctct gaagccccat tatgtccccc tgtaaataat gtagatgtca
     7861 cttccttctt aatattctaa tccatactgt gaaatcgatt ttgcatttat cggtcaaata
     7921 gagcattttg agatagttgg agttaccctg ccaaggatta gaaatctact tcatgttttt
     7981 aaagtacttg ttaaaaatga acgaccctgg cacattctct cataatttta ttccagccat
     8041 gtgaaatctt tcttctaaac actttatcct tgcggaggaa aaaaaaaatg agctgatgag
     8101 ccatttaagc acaaaggggc tttatttaga agattccaag ggggaacttt gaagtaaata
     8161 tataaaacat acttcatcaa tttgcctata aaactaaaag aggaacacag gaatattgat
     8221 aaaataagtc attaaaacac attctttatt tcttgcccag tttaaaagaa agaactaaac
     8281 atccctagag agaggaagag acatagagag agagtgacta atagaaagag ggagagaaga
     8341 aacaaggcac caaggacaaa aagagataat tagacagaac ttgtccaggt tttcacacta
     8401 tgtgctctgt ccagtaccgt acacaagaac ctctttccaa atatttgtcc taaggctcta
     8461 agaagttaag tacgaggctg aaggttgaat acaactgtct ttaatcatta acagtttggg
     8521 gagctacttt taaacgtcta tggaagatgc caagcagtgg taagccagac aatacagagc
     8581 actgcatatc tgtcaagcag ctgaaatatg tttgggcaac ttaatggtga gccacacaaa
     8641 acatccattt gtaacaattt taatacattc aattaagaaa ccaggatttt tattattttg
     8701 cacccataaa aatataacta tattgttcat ttttattgat agagtatgtg tgaatcttat
     8761 tgattatctg taatttacta ttaatgtttt tacagtgact gttttttttg tgtgtgtaaa
     8821 cttaatatat gtcagcaact ggttcctcaa cacaattttt tttagcatta caaaaaaatg
     8881 aacaggtata aaggttctct tttttctaca tcatgttgaa catattttgt tctgaattac
     8941 atagttttaa atgtaatatt aagttttata ttcatatatg tttaacatca aaatcactac
     9001 ttatgacatt gttatcaatt taaaaaatag tatttgacac taggatagca tttaattaaa
     9061 gctaaaaagc ttacacccca tttcatgttg attagtgttt ggactaactc taaaatgtca
     9121 tcaatggaag ctagtcactg aaattatttt atctattgtc atagaatggt gactacccaa
     9181 aaaatataag ttagcattaa atagaagaaa gcgtacgtga ccacaaatcc atgcacaggg
     9241 ttgtgtgaag acaggagaac ctcatttttc tgttttgtct ctttccactg tgtaaaaagt
     9301 ctacatctgt gggctatttc taaattcaaa ttgtcacaat ttgcaatcat aaatgtttag
     9361 catactttgt agaattttga tagttttgta aaagagtgaa aaacaaatgc atatgtaaat
     9421 aaagcagccc atactagcag attcctcaaa tgttaatatg taaataaagc agcccttact
     9481 agcagattca tcaaatgtta atatgtaaat aaagcagtcc ttattagcag atttgtcata
     9541 tgttaagggg agtaatgata aggaggcaac taaatcagga tggtcagtaa ctgatctggg
     9601 tttagaactg tgtttggagc catcaatttt taaatatatg ttctcactat gttattagtt
     9661 gtctgaagaa gcaatcaaga attgctccca gaaaatgagt aagtagccat gaatatatga
     9721 atgctgttta cagaacccat agacctatga atgctcaaaa tgtttgggtt tgtcaaaaaa
     9781 ttacattgta gttatacttg atacttaaaa actgttaata gagtctaaaa taaaagtcgc
     9841 taaaattaaa aaaaaaaaaa aaaaa
//
