LOCUS       NM_011323              11241 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus sodium channel, voltage-gated, type VIII, alpha
            (Scn8a), transcript variant 2, mRNA.
ACCESSION   NM_011323
VERSION     NM_011323.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Espino,C.M., Nagaraja,C., Ortiz,S., Dayton,J.R., Murali,A.R.,
            Ma,Y., Mann,E.L., Garlapalli,S., Wohlgemuth,R.P., Brashear,S.E.,
            Smith,L.R., Wilkinson,K.A. and Griffith,T.N.
  TITLE     Differential encoding of mammalian proprioception by voltage-gated
            sodium channels
  JOURNAL   Sci Adv 11 (2), eads6660 (2025)
   PUBMED   39772670
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Miralles,R.M., Boscia,A.R., Kittur,S., Hanflink,J.C., Panchal,P.S.,
            Yorek,M.S., Deutsch,T.C.J., Reever,C.M., Vundela,S.R., Wengert,E.R.
            and Patel,M.K.
  TITLE     Parvalbumin interneuron impairment causes synaptic transmission
            deficits and seizures in SCN8A developmental and epileptic
            encephalopathy
  JOURNAL   JCI Insight 9 (20), e181005 (2024)
   PUBMED   39435659
  REMARK    GeneRIF: Parvalbumin interneuron impairment causes synaptic
            transmission deficits and seizures in SCN8A developmental and
            epileptic encephalopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11241)
  AUTHORS   Yu,W., Hill,S.F., Zhu,L., Demetriou,Y., Reger,F., Mattis,J. and
            Meisler,M.H.
  TITLE     Dentate gyrus granule cells are a locus of pathology in Scn8a
            developmental encephalopathy
  JOURNAL   Neurobiol Dis 199, 106591 (2024)
   PUBMED   38969233
  REMARK    GeneRIF: Dentate gyrus granule cells are a locus of pathology in
            Scn8a developmental encephalopathy.
REFERENCE   4  (bases 1 to 11241)
  AUTHORS   King,D.R., Demirtas,M., Tarasov,M., Struckman,H.L., Meng,X.,
            Nassal,D., Moise,N., Miller,A., Min,D., Soltisz,A.M., Anne,M.N.K.,
            Alves Dias,P.A., Wagnon,J.L., Weinberg,S.H., Hund,T.J.,
            Veeraraghavan,R. and Radwanski,P.B.
  TITLE     Cardiac-Specific Deletion of Scn8a Mitigates Dravet
            Syndrome-Associated Sudden Death in Adults
  JOURNAL   JACC Clin Electrophysiol 10 (5), 829-842 (2024)
   PUBMED   38430092
  REMARK    GeneRIF: Cardiac-Specific Deletion of Scn8a Mitigates Dravet
            Syndrome-Associated Sudden Death in Adults.
REFERENCE   5  (bases 1 to 11241)
  AUTHORS   Arratia,L.M., Bermudes-Contreras,J.D., Juarez-Monroy,J.A.,
            Romero-Macias,E.A., Luna-Rojas,J.C., Lopez-Hidalgo,M., Vega,A.V.
            and Zamorano-Carrillo,A.
  TITLE     Experimental and computational evidence that Calpain-10 binds to
            the carboxy terminus of NaV1.2 and NaV1.6
  JOURNAL   Sci Rep 14 (1), 6761 (2024)
   PUBMED   38514708
  REMARK    GeneRIF: Experimental and computational evidence that Calpain-10
            binds to the carboxy terminus of NaV1.2 and NaV1.6.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11241)
  AUTHORS   Harris,J.B., Boakes,R.J. and Court,J.A.
  TITLE     Physiological and biochemical studies on the cerebellar cortex of
            the murine mutants 'jolting' and 'motor end-plate disease'
  JOURNAL   J Neurol Sci 110 (1-2), 186-194 (1992)
   PUBMED   1506858
REFERENCE   7  (bases 1 to 11241)
  AUTHORS   Bakalian,A., Kopmels,B., Messer,A., Fradelizi,D.,
            Delhaye-Bouchaud,N., Wollman,E. and Mariani,J.
  TITLE     Peripheral macrophage abnormalities in mutant mice with
            spinocerebellar degeneration
  JOURNAL   Res Immunol 143 (1), 129-139 (1992)
   PUBMED   1565842
REFERENCE   8  (bases 1 to 11241)
  AUTHORS   Kopmels,B., Wollman,E.E., Guastavino,J.M., Delhaye-Bouchaud,N.,
            Fradelizi,D. and Mariani,J.
  TITLE     Interleukin-1 hyperproduction by in vitro activated peripheral
            macrophages from cerebellar mutant mice
  JOURNAL   J Neurochem 55 (6), 1980-1985 (1990)
   PUBMED   2230805
REFERENCE   9  (bases 1 to 11241)
  AUTHORS   Sidman,R.L., Cowen,J.S. and Eicher,E.M.
  TITLE     Inherited muscle and nerve diseases in mice: a tabulation with
            commentary
  JOURNAL   Ann N Y Acad Sci 317, 497-505 (1979)
   PUBMED   289327
REFERENCE   10 (bases 1 to 11241)
  AUTHORS   Zacks,S.I. and Sheff,M.F.
  TITLE     Regeneration and differentiation of minced anterior tibial muscle
            explants from mice with MED myopathy
  JOURNAL   Lab Invest 36 (3), 303-309 (1977)
   PUBMED   839740
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK158609.1, AF049617.1, AK083220.1, AC104834.4 and AK160844.1.
            
            On Mar 7, 2013 this sequence version replaced NM_011323.2.
            
            Transcript Variant: This variant (2) lacks an in-frame exon and
            contains an alternate in-frame exon compared to variant 1. The
            encoded isoform (2) is the same length as isoform 1 but has a
            distinct sequence.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence and transcripts to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF049617.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AK158609.1         62-128
            68-3413             AF049617.1         14-3359
            3414-4535           AK083220.1         1011-2132
            4536-5738           AF049617.1         4482-5684
            5739-7075           AK083220.1         3337-4673
            7076-8181           AC104834.4         98072-99177
            8182-9912           AK160844.1         1-1731
            9913-11232          AK160844.1         1733-3052
            11233-11241         AC104834.4         102229-102237
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.39 cM"
     gene            1..11241
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /note="sodium channel, voltage-gated, type VIII, alpha"
                     /db_xref="GeneID:20273"
                     /db_xref="MGI:MGI:103169"
     exon            1..330
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..5991
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sodium channel protein type 8 subunit alpha; sodium
                     channel protein type VIII subunit alpha; voltage-gated
                     sodium channel subunit alpha Nav1.6; ataxia 3;
                     voltage-gated sodium channel alpha subunit"
                     /codon_start=1
                     /product="sodium channel protein type 8 subunit alpha
                     isoform 2"
                     /protein_id="NP_035453.2"
                     /db_xref="CCDS:CCDS57011.1"
                     /db_xref="GeneID:20273"
                     /db_xref="MGI:MGI:103169"
                     /translation="MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSH
                     REDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKT
                     LFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKN
                     VEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSA
                     LRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGN
                     LRNKCVVWPINFNESYLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAGQCP
                     EGFQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFV
                     LVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAA
                     MATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEGEE
                     KGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIF
                     SFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQCS
                     RSSRIFPSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEATTEVEIKKKGPGSLLVS
                     MEQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKL
                     KEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEM
                     FLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLADVEGLSVLRSFRLLRVFKLAKSW
                     PTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISQECKLPR
                     WHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLA
                     LLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWAKVKVHAFMQAHFKQREADEVK
                     PLDELYEKKANCIANHTGVDIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNP
                     NLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEVPV
                     EQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIF
                     MILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAW
                     CWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVG
                     AIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIDEVNNKTDCEKLM
                     EGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNI
                     YMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLG
                     SKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENIL
                     YWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVS
                     PTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGM
                     SNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHP
                     GSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFE
                     TFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHC
                     LDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVL
                     QRAYRGHLARRGFICRKITSNKLENGGTHREKKESTPSTASLPSYDSVTKPDKEKQQR
                     AEEGRRERAKRQKEVRESKC"
     exon            331..449
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            450..539
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            540..668
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            669..760
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            761..982
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1046
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1188
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1395
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1689
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..2046
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2179
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2418
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2592
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2949
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3420
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3538
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3693
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3867
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..3990
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            3991..4275
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            4276..4329
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            4330..4467
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            4468..4572
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4843
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     exon            4844..11241
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11198..11203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /note="hexamer: AATAAA"
     polyA_site      11215
                     /gene="Scn8a"
                     /gene_synonym="C630029C19Rik; dmu; med; mnd-2; mnd2;
                     NaCh6; Nav1.6; nmf2; nmf335; nmf58; nur14; seal"
                     /note="major polyA site"
ORIGIN      
        1 agccgacctg tcccggactc agcataacta acgaagctgc tgcagaatga gaagatggca
       61 gcgcggctgc tcgcaccacc aggccccgac agtttcaagc ctttcacccc cgagtcgctg
      121 gcaaacatcg agaggcgcat cgccgagagc aagctcaaga aaccacccaa ggcggatggc
      181 agccaccggg aggacgatga agacagcaag cccaagccaa acagtgacct ggaggcgggg
      241 aagagtttgc ctttcatcta cggggacatc ccgcaaggcc tggttgcggt tcccctggag
      301 gactttgacc cgtactattt gacgcagaaa acttttgtag tattaaacag agggaaaact
      361 ctcttcagat ttagcgccac tcctgccttg tacattctaa gtccttttaa cctgataaga
      421 agaatagcta ttaaaatttt gatacattca gtcttcagca tgatcatcat gtgcaccatc
      481 ctgaccaact gtgtattcat gacttttagt aaccctcccg aatggtccaa gaatgtggag
      541 tacacattca cagggattta cacatttgaa tcactggtga aaatcatcgc cagaggtttc
      601 tgcatagacg gcttcacctt cttacgagac ccgtggaact ggttagactt cagtgtcatc
      661 atgatggcat atgtgacaga gtttgtggac ctgggcaatg tctcagcgct gagaacattc
      721 agggttctcc gagctttgaa aactatctct gtaattccag gcctgaagac aatcgtgggc
      781 gccctcatcc agtctgtgaa gaagctgtca gacgtgatga tcctgacggt gttctgcctg
      841 agtgtcttcg ccctgattgg cctgcagctc ttcatgggga accttcgaaa caagtgtgtc
      901 gtgtggccca taaacttcaa cgagagctat ctggagaacg gcaccagagg cttcgactgg
      961 gaggagtata tcaacaataa aacaaacttt tacatggtcc ctggcatgct agaacccttg
     1021 ctctgcggga acagctctga cgccgggcaa tgcccagagg ggttccagtg catgaaagca
     1081 ggaagaaatc ccaactacgg ctacaccagc ttcgacacct tcagctgggc cttcctggca
     1141 ctgttccgcc tcatgactca ggactactgg gagaacttat accagctgac cttacgagcg
     1201 gctgggaaaa cgtacatgat cttctttgtc ttggtgatct tcgtgggttc tttctatttg
     1261 gtgaacttga tcttggctgt ggtggccatg gcttacgaag agcagaacca ggcaacactg
     1321 gaggaggcag agcagaaaga ggcagagttc aaggcgatgc tggagcagct caagaagcag
     1381 caggaggagg cgcaggctgc tgcaatggcc acttcggcgg gcaccgtctc agaagatgcc
     1441 attgaagaag aaggggaaga tggggtaggc tctccgagga gctcatctga gctgtctaaa
     1501 ctcagttcca agagcgcgaa ggagcgtcgg aaccgacgga agaagaggaa gcagaaggag
     1561 ctctctgaag gggaggagaa gggggacccc gagaaggtgt ttaagtcaga gtcggaagat
     1621 ggcatgagaa ggaaggcctt ccggctgcca gacaatagga tagggaggaa attttccatc
     1681 atgaatcagt cgctgctcag catcccaggc tcgcccttcc tttcccgaca caacagcaaa
     1741 agcagcatct tcagctttcg gggtcctgga cggttccggg accccggttc ggagaacgag
     1801 ttcgcagacg acgagcacag cacagtggag gaaagtgagg gccggcgcga ctcgctcttc
     1861 atcccgatcc gcgcccgcga gcgccgcagc agctacagcg gctacagcgg ctacagccag
     1921 tgcagccgct cgtcgcgcat cttccccagc ctgcggcgca gcgtgaaacg caacagcacc
     1981 gtggactgca atggcgtggt gtcgctcatc gggcctggct cgcacatcgg gcggctcctg
     2041 cctgaggcaa caactgaggt ggagattaag aagaaaggcc ctggatcgct cttagtctcc
     2101 atggaacaac tcgcctccta cggacggaag gacagaatca acagtataat gagcgttgtc
     2161 acaaacacac tagtggaaga gctggaagag tctcagagaa agtgcccacc atgctggtat
     2221 aagtttgcca atactttcct catctgggag tgtcacccct actggataaa actgaaggag
     2281 attgtgaacc tgatcgtcat ggaccctttc gtagacttag ccataaccat ctgcattgtc
     2341 ctgaacacac tatttatggc aatggagcac catcccatga cgccacaatt cgaacatgtc
     2401 ctggccgtag ggaacctggt gttcactggg atcttcacgg cggaaatgtt cctgaagctc
     2461 atagccatgg atccctacta ttacttccaa gaaggctgga acatttttga cggatttatc
     2521 gtctccctca gtttaatgga gctgggcctt gcagacgtgg aggggctctc agtgctgcga
     2581 tctttccgat tgctccgagt cttcaaattg gccaagtcct ggcccaccct gaacatgctg
     2641 atcaagatca ttgggaactc cgttggcgcc ctgggcaacc tgaccctggt gctggccatc
     2701 attgtcttca tctttgccgt ggtggggatg cagctctttg gaaagagcta caaggagtgc
     2761 gtctgtaaga tcagccagga gtgcaagctc ccgcgctggc acatgaacga cttcttccac
     2821 tccttcctca tcgtcttccg agtgctgtgt ggggagtgga tcgagaccat gtgggattgc
     2881 atggaagtgg ccggccaggc catgtgcctt atcgtgttca tgatggtcat ggtcatcggc
     2941 aacctggtgg tgctgaactt atttctggcc ttgcttctga gctccttcag cgcagacaat
     3001 ctggcggcca cggacgacga cggggaaatg aacaacctgc agatatcggt gatccggatc
     3061 aagaagggcg tggcctgggc caaagtgaag gtgcatgcct tcatgcaggc acacttcaag
     3121 caacgggagg ctgatgaagt gaaaccttta gacgagctgt atgagaagaa ggccaactgc
     3181 atcgccaacc acaccggcgt ggacattcac aggaacggcg acttccagaa aaatggaaat
     3241 ggcaccacta gcggcatcgg cagcagcgtg gagaagtaca tcatcgacga ggaccacatg
     3301 tccttcatta acaacccaaa cctgaccgtg cgggtgccca ttgctgtggg cgagtctgat
     3361 ttcgagaacc tcaacacaga ggatgttagc agcgagtcag accccgaagg cagcaaagac
     3421 aaactggacg ataccagctc ctcagaaggg agtaccatcg acatcaagcc tgaggtggaa
     3481 gaagtcccag tggagcagcc tgaggaatac ttggatccag acgcctgctt cacggagggt
     3541 tgtgtccaga ggttcaagtg ctgccaggtc aacatcgagg aaggactagg caagtcgtgg
     3601 tggatcctgc ggaaaacctg cttcctcatt gtggagcaca attggtttga gaccttcatc
     3661 attttcatga ttctcctcag cagtggagcc ctggccttcg aggacatcta cattgagcag
     3721 aggaagacca tccgtaccat cctggagtat gcggacaagg tcttcaccta catcttcatc
     3781 ctggagatgt tgctcaaatg gacagcctat ggcttcgtca agttcttcac caatgcctgg
     3841 tgctggttgg acttcctcat tgtggctgtc tctttagtca gccttatagc taatgccctg
     3901 ggctactcgg aactaggtgc cataaagtcc cttaggaccc taagagcttt gagaccctta
     3961 agagccttat cacgatttga agggatgagg gtggtggtga acgccttggt gggcgccatc
     4021 ccctccatca tgaacgtgct gctggtgtgt ctcatcttct ggctgatttt cagcatcatg
     4081 ggcgttaacc tgtttgctgg gaaataccac tactgcttca acgagacttc agaaatccgg
     4141 ttcgaaatcg acgaggtgaa caataaaacc gactgtgaga agctcatgga gggcaacaac
     4201 acagagatcc gatggaagaa cgtcaagatc aacttcgaca acgtcggggc aggctacctg
     4261 gcccttcttc aagtggcaac cttcaaaggc tggatggaca tcatgtatgc agctgtagat
     4321 tcccgaaagc cggacgagca gcctgattat gagggcaaca tctacatgta catctacttc
     4381 gtcatcttca tcatcttcgg ctccttcttc accctcaacc tgttcatcgg tgtcatcatc
     4441 gacaatttca atcaacagaa gaaaaagttt ggaggtcagg acatcttcat gacagaggaa
     4501 cagaagaagt actacaacgc catgaagaag ctaggctcca agaagccaca gaagcccatc
     4561 ccccgacctt tgaacaaaat ccaagggatt gtctttgatt tcgtcacgca acaagccttc
     4621 gacatcgtga tcatgatgct tatctgcctt aacatggtga ccatgatggt ggagacagac
     4681 acacagagca agcagatgga gaacattctc tactggatta atctggtctt cgtcatcttc
     4741 ttcacctgcg agtgtgtgct caaaatgttt gccttgagac actactattt caccattggc
     4801 tggaacatct ttgactttgt ggtggtcatt ctctccattg tgggaatgtt cctggctgat
     4861 atcattgaga agtacttcgt ctccccgacc ctattccgcg tcatccgatt ggcccgcatc
     4921 gggcgcatct tgcgtctgat caagggcgcc aaagggatcc gcaccctgct ctttgcctta
     4981 atgatgtcgc tgcccgccct gttcaacatc ggcctcctgc tcttcctggt catgttcatc
     5041 ttctccatct ttgggatgtc caacttcgcg tacgtgaagc acgaggccgg cattgatgac
     5101 atgttcaact tcgagacgtt tggcaacagc atgatctgcc tgttccagat cacgacctct
     5161 gctggttggg atggcttact gctgccaatc ctgaaccgcc cccctgactg cagcttggac
     5221 aaggagcacc caggaagtgg cttcaaaggg gactgcggga acccctccgt gggcatcttc
     5281 ttcttcgtca gctacatcat catctccttt ctgatcgtgg taaacatgta cattgccatc
     5341 atcttggaga acttcagcgt agccacagag gaaagcgccg atcctctgag cgaggacgac
     5401 ttcgagactt tctatgagat ctgggagaag tttgatcctg atgccaccca gttcatcgag
     5461 tactgtaagc tggccgactt tgccgacgcc ctggagcatc cgctccgagt acccaagccc
     5521 aacaccatcg agctcatcgc catggacctg cccatggtga gcggagatcg aatccactgc
     5581 ttggacatcc ttttcgcctt caccaagcga gtcctgggag acagtgggga gttggacatc
     5641 ctgcggcagc agatggagga gcggttcgtg gcgtccaatc cttccaaagt gtcttacgag
     5701 cctatcacga ccactctgcg gcgcaagcag gaagaggtgt ctgcagtggt cttgcagcgt
     5761 gcttacaggg gacacctggc taggcggggc ttcatctgca gaaagatcac ttccaacaag
     5821 ctggagaatg gaggcaccca ccgagagaag aaggagagca ccccgtccac agcctccctc
     5881 ccctcttacg acagcgtgac aaagcccgac aaggagaagc agcagcgggc agaggaaggc
     5941 aggagggaaa gagccaagag gcaaaaagaa gtcagggagt ccaagtgcta gaggagaggg
     6001 taagggaagc ttacgctggc tgaacacggg caagtgaaag cttgtttaca aacttccgaa
     6061 tctcacggac acagagcagc tgcggagatg ctgtactgga agacctatac caaacatagt
     6121 ctgcttacac gtgacacggt tgcatcctga gcggtgacct gctggggaca aaggaccctg
     6181 ctccctggac tcacagattt tttctatcgc ttgggcagac gttactgcat gttccacact
     6241 cggtcaatgc aacttaggag taaacttaac aggatacaaa cagaggcggc tgccaggacc
     6301 agcagatcgc cgctgcagcc aaatggattc tatttttttt ttcattttgt tgattctcag
     6361 aagcagaaag catcacttta aaagtttgtt tgttcatgca aacaatattt gcattcttac
     6421 attagttaag ctaagcagca aaaagaaaca cacacacata cagacacaca aagacacaca
     6481 cagacacata cacacacaca cacacattca gcctatgtca ctaatcgtct gtttctttaa
     6541 cataacagca tcttctccac gtgggtggca catggtttgg agacggtggg ggaaacaatc
     6601 agggtttcag gctgaggagg acttgctcag gccaattcca aatattgtgc tcgttcaatg
     6661 cgtagaaatg acttgcatga tggcatgctg tgttcagaag tcatgcatga ggtccataca
     6721 ccaacagaca ctagcaatcc tgtccctcgc attggctcag ccttcggaca ggacccagcc
     6781 ctgcactgtt cactgtattt ggagaaatgg taagagttcc ataccggctg caatcctctc
     6841 agtgtacaag tctttcatac acctctgggt agggagacat aattaaccaa ttgaccacta
     6901 ccaacaaaaa caacccaacc aaacaagcag atggatccgc tgcatataca tgtttgacag
     6961 acatctctga catacagcca ttgtcgtaca ttttgctgct ctgtccaata catggggaga
     7021 ctctggcccc gaatggcttg tactattaat gtcactgtaa aaccaaatcc tagggctaaa
     7081 aaaaaaaaaa aaaagaaaaa agaaaaaaaa aagaaaaata ctaatagaaa aaagagttca
     7141 tttgtatctt gcaatatcct gttgattgtt tagccttcat cctggagaac tgaccctggg
     7201 gtagagataa aagtgctatg tattgtagca tgttacctct agggggcctt tggggggctt
     7261 acagaaacct tgtggggggt ttggggtgct tattgcattc agtgtcatgt ctgtctgctt
     7321 ttctccaggc tcaggtagat acggaaaggg gttcagatat tctgaactgc cctgtcctgc
     7381 agaggggttg tcacaagctc ctggctgcac ggttcttcct gcttctgcca tatccctcca
     7441 gccagcaggg ctccccttcc cagccgctca gggctggcaa ggacagcagt gttggctgag
     7501 ataaggctgt aggctcttcc atttgtagaa taacgctctc taacaggacc tgatggaaag
     7561 atggagctgc ctctggacag agcccacttg gtcttgtttc tacgacggct tttgtgagaa
     7621 agggactcag tagtcattct ttagggtgtc acagcttccc ctggcctttc ctttcagctc
     7681 accttgcttt ctgcttagtg gcctgcccac tgttgttggg ggatggcttc tagaggggcg
     7741 tcatatcttc cattccatcc cccactttat agaagacagt tcttagtcca gggtgtctca
     7801 gctgaccaca aggtctcaaa gccaagatgc tgcactcgaa tgccagaaag tctctctgaa
     7861 ccaaatcaac acaaactctc cctgagcata gtatggaggg aatcgagtgg cccatcagta
     7921 gacacatcag atgcaccagt ccgccttgca gtagaagcac tttggatgta atctcacagt
     7981 ccacctgact agttttgcat agaacaaacc acagccagtc agtgagcaga gctttctatc
     8041 ttcttggacc ggtagacact gtccaactct cccagctcag agagtgtgtc cgtgtcacca
     8101 cagctaggac aattctagag cgccacctgc aggcaacatc gcaaacagca caaaacagga
     8161 gctgacctgt ggttattctt tttaagagaa ttataaatac tgtaatatat aattttattt
     8221 tattggcatg cctttttgta aatacaaatt actaacttat taaataggaa atggcttctg
     8281 gcttacgcca ttgtcatggt tatttttatt ttttatactt tcctttcttc ttagaattta
     8341 gaagctgtca gccaatgtat acccctaacc agacagacag acaaaaaatt ttttattgct
     8401 aatggtattt tccaaaacaa caaaatatat atatattttt gttccaatgt gcaataaatt
     8461 ctaaccactt ctatgcaaga tttggctaaa ctcaatcatt gttcttagat cttgagatgg
     8521 gcaacagcta tttgatcctg ctaggaacac caagcgccat gctagtaatg ttcagcgatt
     8581 agatctgacc ctcctacccc agctcctgac ccagcccagc aggctgtgcc tcagtttctc
     8641 acaccctcca acagcctcgc agggaagaag ccacctccga gtctgcttgc tctaactttc
     8701 actctgatca gacctctccc agcaatgccc ctcaccttcc atctgtccac ccagccactc
     8761 tctgcccgcc tcgctctctc tgtcccactc catgatcctt tcttgtgaac cacaccatca
     8821 ccagcagttc acacccgcag ttaacaggca tcgaacctta aagaatcagc tacggcaggt
     8881 tttggttggt ttggttttgt tttgcgtcct cattgcgtga gcaagtggca gggctttgcc
     8941 aaatattcat gaagcctgtc aaagagcggc agcagccagt accactgcac atatatatat
     9001 agatatataa atataatata aatatttatt ttttgtagcc gggtccttgg tgcagtattt
     9061 atattccacg atcctccttt aaaaataata ataataataa taaaaaggac aaaaaagcaa
     9121 aaagtcatgt gatgctgttc atttaaaacg cagagccaaa gcccctgagc agtagtcgcc
     9181 atgactactg gttgtgtgaa aacactccct tcttccttaa cttccctgtg gcttagggtg
     9241 agaggctggg agtcacaggg cagagggtgg gagcaggaaa gccgagtctg agtgatctgc
     9301 tctcaccatc tggaaatgag ccacttgggc atctgaagct gcgcacagag agacaagctc
     9361 cccctcaggg cccctgaagc gacatagagt gcccaaaggg cttctctcat caccacaacc
     9421 cacgtaaact gtaagagacc catagtgtgc tagtgagtgc atggagcctg atgctgccct
     9481 aaacgatacc tgatctttag aaaagtaaat cactattagc caaaaggaaa caaaatttac
     9541 aaaacaaaca aacaaacaaa aaatacggcc ctttttaacc acacaaggga agcccgtctt
     9601 gctgtgtgtc tgtttgactt tccagttatt gaggtctggg aactgggacc ctggagcttt
     9661 ggggaaggag aaaggaaaac agagcccccc ccccccaatt ctaagtaggt actgcagaga
     9721 aaagccaggt cctgaccccc caggtccctg cttactgatt gcatgtggca ttacccctta
     9781 gactggaccc ccctccctca ccgtttccac ccagcagagc cccccagaga tgacttcaac
     9841 caacctagct ggtctggcct tacttctgcc ttgttcatgg ttatgattta agaaagaaag
     9901 aaaaaaaaaa aacctctcct gaccccccac ctcccaaggc tagcgcaagc acgctgcccg
     9961 gcagcttcca gtccaccagc tcgtgctggc cctgccttcc atccactccc attatcagac
    10021 cggcagactg gggctgtccc cttcacattt gtgcagggga gggggcatcc cttggggaca
    10081 tccctctcca ggccatgtct cctggtctag gaacactcag gaggatcttg aagtgcccac
    10141 tctagtccac tctagtagct gaactagaga gagtcggctg gtaggtcagt ccctgtcctt
    10201 gtccctcagc tccagcctcc aggccttctg tggaggtgaa attgccatgg gtccggacat
    10261 ggtgacctga accctgggca tcttctggaa cgatgcctct ttcttttctc agatgttgcc
    10321 caaagttttg caaaaagctt ccttcatttt caggtactcc cccgccccct aaagaattag
    10381 ctgccgtcag tcagccaggg gggtcaggag aagaggaagt ctgggttcag aggacaagac
    10441 ttgcaccccg gcctgtgctt cttgcctctt ctcaagactg ccaagatttc ctccattaaa
    10501 acactcctga ggcacccctg cccctgaccc cactgctgtt tgctgtacat agaggctaag
    10561 tggggtgaag tgggcaggtg tgcacatgta tgtgtgtgtg tgtgtgaaga atgtacatag
    10621 gtaaagggga gcgtggtcca gtggttccag aagagggaca gaactgtgtt ccatccctag
    10681 cttgaccact ggctcgctgt gtggccttgg gcaagtcact taacctctct gtgcctcagt
    10741 ttccccatct gtaaaatggg ggtaataata ctgacctacc tcacaggggt gttgtgaggc
    10801 tttaactaga tgttttgtaa agcgttctga gatacagaga caaaggcgcc agggaagggc
    10861 aaagtattct ttggacttga ggaagaggaa ctagtaccat aaacgctgct attctgagag
    10921 ccattttaaa ctgcactgct ccaccacccc ccgcttctca tcaccaggac agcaggtcga
    10981 gaaatgtctt tccttgcact tgcttctggg gtttgtgatt cttctgattt ttccctttgc
    11041 tgtatctcct tccttacccc tccactcgtt ccctgttctg ttaatgcgga aatggcagaa
    11101 atgcttgaga aatgagaatg tataagtgga ttggaagttt atagtctgga ttttcaattt
    11161 tactttgtac tgtacatctt tttactttag aatttgcaat aaagggttac gtcaatcttg
    11221 ttttcaactc tcctcttgga a
//
