LOCUS       NM_009135               7333 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sodium channel, voltage-gated, type VII, alpha
            (Scn7a), mRNA.
ACCESSION   NM_009135
VERSION     NM_009135.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Lowenstein,E.D., Misios,A., Buchert,S. and Ruffault,P.L.
  TITLE     Molecular Characterization of Nodose Ganglia Development Reveals a
            Novel Population of Phox2b+ Glial Progenitors in Mice
  JOURNAL   J Neurosci 44 (28), e1441232024 (2024)
   PUBMED   38830761
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Hou,C., Dolivo,D., Rodrigues,A., Li,Y., Leung,K., Galiano,R.,
            Hong,S.J. and Mustoe,T.
  TITLE     Knockout of sodium channel Nax delays re-epithelializathion of
            splinted murine excisional wounds
  JOURNAL   Wound Repair Regen 29 (2), 306-315 (2021)
   PUBMED   33378794
REFERENCE   3  (bases 1 to 7333)
  AUTHORS   Sakuta,H., Lin,C.H., Hiyama,T.Y., Matsuda,T., Yamaguchi,K.,
            Shigenobu,S., Kobayashi,K. and Noda,M.
  TITLE     SLC9A4 in the organum vasculosum of the lamina terminalis is a
            [Na+] sensor for the control of water intake
  JOURNAL   Pflugers Arch 472 (5), 609-624 (2020)
   PUBMED   32372285
REFERENCE   4  (bases 1 to 7333)
  AUTHORS   Sakuta,H., Lin,C.H., Yamada,M., Kita,Y., Tokuoka,S.M., Shimizu,T.
            and Noda,M.
  TITLE     Nax-positive glial cells in the organum vasculosum laminae
            terminalis produce epoxyeicosatrienoic acids to induce water intake
            in response to increases in [Na+] in body fluids
  JOURNAL   Neurosci Res 154, 45-51 (2020)
   PUBMED   31150667
REFERENCE   5  (bases 1 to 7333)
  AUTHORS   Nomura,K., Hiyama,T.Y., Sakuta,H., Matsuda,T., Lin,C.H.,
            Kobayashi,K., Kobayashi,K., Kuwaki,T., Takahashi,K., Matsui,S. and
            Noda,M.
  TITLE     [Na+] Increases in Body Fluids Sensed by Central Nax Induce
            Sympathetically Mediated Blood Pressure Elevations via H+-Dependent
            Activation of ASIC1a
  JOURNAL   Neuron 101 (1), 60-75 (2019)
   PUBMED   30503172
  REMARK    GeneRIF: Nax channels are expressed in specific glial cells in the
            organum vasculosum lamina terminalis which detects increases in
            Na(+) in body fluids.
REFERENCE   6  (bases 1 to 7333)
  AUTHORS   Tarantino,L.M., McClearn,G.E., Rodriguez,L.A. and Plomin,R.
  TITLE     Confirmation of quantitative trait loci for alcohol preference in
            mice
  JOURNAL   Alcohol Clin Exp Res 22 (5), 1099-1105 (1998)
   PUBMED   9726281
REFERENCE   7  (bases 1 to 7333)
  AUTHORS   Knittle,T.J., Doyle,K.L. and Tamkun,M.M.
  TITLE     Immunolocalization of the mNav2.3 Na+ channel in mouse heart:
            upregulation in myometrium during pregnancy
  JOURNAL   Am J Physiol 270 (2 Pt 1), C688-C696 (1996)
   PUBMED   8779936
REFERENCE   8  (bases 1 to 7333)
  AUTHORS   Seldin,M.F., Rochelle,J.M., Tomlinson,M.G. and Wright,M.D.
  TITLE     Mapping of the genes for four members of the transmembrane 4
            superfamily: mouse Cd9, Cd63, Cd81, and Cd82
  JOURNAL   Immunogenetics 42 (5), 422-425 (1995)
   PUBMED   7590978
REFERENCE   9  (bases 1 to 7333)
  AUTHORS   Felipe,A., Knittle,T.J., Doyle,K.L. and Tamkun,M.M.
  TITLE     Primary structure and differential expression during development
            and pregnancy of a novel voltage-gated sodium channel in the mouse
  JOURNAL   J Biol Chem 269 (48), 30125-30131 (1994)
   PUBMED   7982916
REFERENCE   10 (bases 1 to 7333)
  AUTHORS   Potts,J.F., Regan,M.R., Rochelle,J.M., Seldin,M.F. and Agnew,W.S.
  TITLE     A glial-specific voltage-sensitive Na channel gene maps close to
            clustered genes for neuronal isoforms on mouse chromosome 2
  JOURNAL   Biochem Biophys Res Commun 197 (1), 100-104 (1993)
   PUBMED   8250913
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928720.6 and AL928623.5.
            
            On Sep 7, 2007 this sequence version replaced NM_009135.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L36179.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AL928720.6         75895-76095         c
            202-452             AL928720.6         48306-48556         c
            453-571             AL928720.6         43381-43499         c
            572-661             AL928720.6         43055-43144         c
            662-790             AL928720.6         34888-35016         c
            791-882             AL928720.6         33144-33235         c
            883-1089            AL928720.6         28560-28766         c
            1090-1159           AL928720.6         20197-20266         c
            1160-1301           AL928720.6         17339-17480         c
            1302-1508           AL928720.6         5038-5244           c
            1509-1605           AL928623.5         178681-178777       c
            1606-1844           AL928623.5         177366-177604       c
            1845-2015           AL928623.5         174397-174567       c
            2016-2372           AL928623.5         173508-173864       c
            2373-2807           AL928623.5         171218-171652       c
            2808-2913           AL928623.5         168475-168580       c
            2914-3068           AL928623.5         166171-166325       c
            3069-3242           AL928623.5         163197-163370       c
            3243-3350           AL928623.5         161396-161503       c
            3351-3629           AL928623.5         157680-157958       c
            3630-3683           AL928623.5         156932-156985       c
            3684-3821           AL928623.5         156370-156507       c
            3822-3926           AL928623.5         154529-154633       c
            3927-4197           AL928623.5         153740-154010       c
            4198-7333           AL928623.5         147091-150226       c
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 39.21 cM"
     gene            1..7333
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /note="sodium channel, voltage-gated, type VII, alpha"
                     /db_xref="GeneID:20272"
                     /db_xref="MGI:MGI:102965"
     exon            1..201
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            202..452
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..5261
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /note="sodium channel, voltage-gated, type VI, alpha
                     polypeptide; nax channel; sodium channel Nav2.3"
                     /codon_start=1
                     /product="sodium channel protein type 7 subunit alpha"
                     /protein_id="NP_033161.2"
                     /db_xref="CCDS:CCDS16081.1"
                     /db_xref="GeneID:20272"
                     /db_xref="MGI:MGI:102965"
                     /translation="MLTSPEPKGLVPFTTESLELIENHIAKKCNEDPEEEEGLKPSRN
                     LEAGKRLPIPYGTLPRGTVSEPLEDVDPYYYVKRNTFMVLNRSRVIFRFNAVSIFCTL
                     SPLNSLRRAAIKALVHPLFRLLILISVLTDSILMCMSNLPEWILAIENTLLGIYAFEI
                     LVKVIARGIWAGSFSFLGDLWNWLDFSVTLFELITRFSPLSSFLMLKTIRTFRILKII
                     PLNHGLQSIVMTLAQCLKKLFGAIALALFFLAVFSLLGMGLFMGNLKHKCLRWPEENE
                     NETLHNRTGSLNYSPERINFYYMEGAKYALLCGNRTDAGQCPEGYVCVKEGTNPDNGF
                     TSFDNFGWSLLAMFRLMTQDYPELLYHQILYASGKVYMIFFVMISFWFAFYLTSLFLG
                     ILTMTYEKEKQRACEESGGLDPKCQQTVKELDEENDAAEMETTQIEMKKRSPTSINTT
                     LDILEDTTLGHREEPETSRKKCPICWHKFIKTCFIWKCSPCWVKLNEFADRVITHPLA
                     DLFLVICIVLNICFLALEHFPMSEELRSLLHVGNLVFIGIYTIEMILKIIAMHPYGYF
                     QISWNIFDSILVVLELTEILLADVEGLAVLITVPLIFIKLGKYGPPFKSLMRILGSSL
                     MALKDLVLLLCIFVYFSAVFGMKLFGRSYKDCVCHIKEDCQPQRWHMSDFLHAYMTVF
                     RILCGEWIETLWECMEVAGQAWCIPFYMMVILIGNLLILYLFVTLVSSFSYYDATSEV
                     NKEAKNLQLAMARIKSGINSMLLKLMCTERSVPTEATDQICDPSVKENISGHTLSELS
                     NTQTFLRYKDQSSSTEKTPVTESESQSLIASPSASETVPIASGESDIENLDNKETRSK
                     SGNGGSKEKMKQSSSSECSTVDIAISEEEEMVYEHEKSKLHKNGYERKSSTGQISRES
                     RNGKIWKNIRKTCCKIVENSWFECFIGLVTLLCTGTLALEDIYIDQRKTTKILLEYAD
                     MIFAYIFILEMLLKWVAYGFKAFFSNNWYKLDFMVVIVFCLSLIGKTREDLNPLTSIK
                     FLRALRVLSQFERMKVVLRALIKTTLPTVSVFLVCLMIWLLFSVIGVQLFAGKFYECI
                     DPTKGERFPVFEVMNKSQCEKLLFNESMPWENAKLNFDNVGNGFLSLLQVATFNGWIS
                     IMNSAIDSVGVNMQPSFEYNLYMYSYFIIFVIFGLFLPLCMLIGVIIRNFNKQKIKQG
                     GSNIFITVKQKKQYRALKKLLYADVQKPTPRPRNKFQGFLFDLVTHRVFNVIIILLIC
                     FQATTIMIQKDEQSPQMETAIFWMNSIFVMLFTLECILKLTAFRCHYFTSAWNVHDFM
                     VVIFSITGLLLPLTIGQYFVPPSLVQLILLSRVIHILRPGKGPKVFHDLMLPLILALP
                     ALLNISLLIFLVMFIYAIFGMYNFAYVKKEAGINDVSNFETFGSSMLCLFQVTTFSGW
                     DGMLDAIFNSQWSDCDPDKINPGTQVKGDCGSPSVGISYFVSYILISWLIIVNMYIVL
                     IMEFLSIPSQKKSRTLSEDDFRRFFRVWNRFDPDRTQYIDSSKLSDFAAALDPPLFMA
                     KPNKGQLVAMDLPMAAGDRIHCLDILLAFTKRVMGKDERVEKILSEIESGFMLANPFK
                     ITYEPITTTLKRKQEAVSATIIQRAYKSYRLRQNDKNVSDTPAIDDRRDDLTSKGAHS
                     GKIEEKASIQTQI"
     exon            453..571
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            572..661
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            662..790
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            791..882
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1089
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1159
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1301
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1508
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1605
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1844
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2015
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2372
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2807
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2913
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            2914..3068
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..3242
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3350
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3629
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3683
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3684..3821
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3926
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..4197
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     exon            4198..7333
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7312..7317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /note="hexamer: AATAAA"
     polyA_site      7333
                     /gene="Scn7a"
                     /gene_synonym="1110034K09Rik; NaG; Nav2; Nav2.3; Nax;
                     Scn6a"
                     /note="major polyA site"
ORIGIN      
        1 gtctgtaggt ggtctctggg tctgtggagc tagcctggtg gctgagtgtt tagctggaag
       61 cagcagtgga ccgcaaccac attgcaacaa cctccgtagt agagatctga gaagacaagc
      121 ccaggagagc aaagggctct cgtgagcctt gcatctgggg ttcttgctgg agttttagtg
      181 aagactagca tttgacagca agtataaaac cgaaaatgtt gacttcccca gagccgaagg
      241 gccttgtccc atttacaaca gagtcacttg aacttataga aaatcacatt gctaaaaaat
      301 gcaatgaaga ccccgaagaa gaagaaggtt taaaaccaag tcgtaatcta gaagctggca
      361 aaagacttcc aattccctat ggaaccctcc ctcgaggaac cgtgtcagag cccttggaag
      421 atgtggatcc atactactat gttaagagaa atactttcat ggtcttaaac agaagcagag
      481 tcatcttcag gttcaatgcg gtttccatct tctgcacatt gtctcctcta aactccctca
      541 gaagagcagc tatcaaggct ttggtgcatc ccctttttcg cctgctgatt ttaatcagcg
      601 ttctcactga cagcatactt atgtgcatga gtaatctacc agaatggata ttggcaatag
      661 agaatacttt gcttgggatt tacgcatttg aaatacttgt aaaagtcatt gcaagaggta
      721 tctgggcagg ttcattttcc ttccttgggg atctttggaa ctggcttgat ttcagtgtaa
      781 ctttgttcga gctaatcaca aggttttcac ctctaagctc ctttttaatg cttaaaacta
      841 tcagaacttt ccgaattttg aagattatcc ctttgaacca cggcctgcag tctattgtga
      901 tgacactggc ccagtgtttg aagaaactat ttggtgccat tgccctagct ctgttttttc
      961 tggctgtgtt ttcactactt ggaatgggcc tcttcatggg caacctgaag cacaaatgtc
     1021 tgcggtggcc agaagaaaat gaaaatgaaa cgctgcacaa cagaactgga agccttaact
     1081 atagtccaga aagaataaac ttctactaca tggaaggagc gaaatatgct ctcctttgcg
     1141 gcaacaggac agatgctggc cagtgtccgg aaggttatgt gtgtgtaaaa gaaggcacaa
     1201 atcctgacaa tggcttcaca agttttgaca actttggctg gtcccttctt gctatgtttc
     1261 gattgatgac acaggattac cctgaattac tttatcacca gatcctttat gcttcaggaa
     1321 aggtctacat gatatttttt gttatgatca gtttctggtt tgccttctat ttgacaagtt
     1381 tgttcttggg catactcact atgacctatg aaaaggaaaa gcagagagcc tgtgaggaat
     1441 ctggaggcct tgatcccaaa tgtcaacaga cagtgaaaga actcgacgaa gaaaatgacg
     1501 cagctgagat ggaaactaca caaatagaaa tgaagaaaag atccccaact tctataaaca
     1561 ccacactgga tatactggaa gacactaccc tcggacacag agaagaacca gaaacatcca
     1621 ggaagaaatg cccaatatgt tggcataagt ttattaaaac ctgcttcatc tggaaatgct
     1681 ctccctgttg ggtaaaactg aacgagtttg ctgatagagt tataacacac ccattggctg
     1741 atctttttct tgtcatctgc atcgttttaa acatatgctt cctcgcccta gaacattttc
     1801 caatgagcga ggagctcagg tctctccttc acgttggaaa tttggttttt attggaattt
     1861 acacaataga aatgattttg aagataatcg ctatgcatcc atatgggtat tttcaaataa
     1921 gctggaatat ttttgacagt atacttgtgg ttttggagtt aacagaaatt ttactagcag
     1981 atgttgaagg actagctgtt ttaataacag tcccattgat atttataaaa ctggggaagt
     2041 acggaccacc atttaagagt ttgatgcgca tccttggtag ctcattgatg gccctgaaag
     2101 atttggtcct gttgctctgc atattcgttt acttctctgc tgtgttcggc atgaagctgt
     2161 ttggtcgaag ttacaaggat tgtgtctgcc acataaagga agactgccaa ccccaacgct
     2221 ggcacatgag tgacttcctt catgcctaca tgaccgtgtt ccgaatcctc tgtggagagt
     2281 ggatagagac attatgggag tgtatggagg ttgcaggcca ggcctggtgt attccttttt
     2341 acatgatggt cattttaatt ggaaacttat tgatacttta cctctttgtg acattggtga
     2401 gctctttcag ttactatgat gctacatcgg aagtgaacaa agaagccaaa aaccttcagc
     2461 ttgccatggc aaggataaag tcgggaataa actccatgct tcttaaatta atgtgcacag
     2521 aaagaagtgt tcctacagaa gcaacagacc aaatatgtga tccaagtgtt aaagagaata
     2581 tttctggcca tactctttct gaactgagca acacccaaac tttcctcaga tataaggacc
     2641 agagcagcag cactgagaaa actccagtga ctgaatctga gagtcaatct ctgattgcta
     2701 gtcccagtgc ctctgaaact gtgccgattg cttcaggaga atctgatata gaaaatctgg
     2761 ataacaagga gactcggagc aagtctggga atggaggcag taaagagaaa atgaagcagt
     2821 ctagctcatc tgagtgcagc acagttgata tcgctatttc tgaagaagaa gaaatggtct
     2881 atgaacatga aaagtcaaag cttcataaaa atggttatga acgcaaatct tcaactggtc
     2941 aaatcagtag agaatctagg aatggaaaga tttggaaaaa catcaggaaa acttgctgca
     3001 agatagtaga gaacagctgg tttgagtgtt tcattggcct ggtcactctg ctctgcacag
     3061 gcactctggc tcttgaagac atatatattg atcagagaaa aaccactaaa atcttactgg
     3121 aatatgcgga catgatattt gcatacatct tcattctgga gatgcttctc aagtgggtgg
     3181 cttatggctt taaagccttt ttctccaaca actggtacaa actggacttt atggttgtta
     3241 tcgtgttttg tcttagctta ataggcaaaa ctcgagaaga tctgaaccct ctgacatcaa
     3301 taaagttcct tcgagcacta agagttctgt cgcagtttga aagaatgaag gtggtcctga
     3361 gagctttgat aaaaacaacc ttacccactg tgagcgtgtt tctagtctgc ctaatgatct
     3421 ggctgctttt cagtgttatt ggagtgcagt tatttgctgg caagttctat gaatgcattg
     3481 acccaacaaa gggagaaaga ttccctgtat ttgaagttat gaataaaagt cagtgtgaaa
     3541 aactgttatt caatgaatca atgccgtggg agaatgcaaa actgaacttt gataatgttg
     3601 gaaatggttt tctttcttta ctccaagtgg caacatttaa tggatggatc agtattatga
     3661 attcagcaat tgattctgtt ggtgtaaaca tgcagcccag ctttgagtac aacctctaca
     3721 tgtatagtta ctttatcatc tttgttatct ttggattatt tcttcctctc tgtatgctga
     3781 ttggtgttat tattcgcaat ttcaacaagc agaaaattaa gcagggagga tcaaacatct
     3841 ttataacagt aaaacagaaa aaacagtacc gggccctgaa gaagctcttg tatgcagacg
     3901 tccagaaacc aacaccccgc cccagaaaca aattccaagg cttccttttt gacctagtaa
     3961 cacaccgtgt ctttaatgtt atcatcatac ttcttatctg tttccaagca acaaccatta
     4021 tgatacaaaa ggatgagcag agtccacaaa tggaaactgc catcttctgg atgaactcca
     4081 tttttgtcat gctgttcact ctggaatgca tactgaagct cactgccttc cgttgccact
     4141 acttcaccag tgcatggaat gttcatgact ttatggtggt cattttctcc attacagggc
     4201 tgctgctacc cttgacaata ggacaatact ttgtgcctcc ttccctggtg cagctgattc
     4261 ttctctctcg agtcatccac atcctgcgtc ctgggaaagg accgaaggtg ttccatgacc
     4321 tgatgcttcc cttgattctg gcgctcccag cattgctgaa cattagtctt ctcatcttcc
     4381 tggtcatgtt catctacgcc atctttggaa tgtacaactt tgcctatgta aagaaagaag
     4441 ccggaattaa tgatgtgtcc aactttgaga cctttggaag cagtatgctc tgtctcttcc
     4501 aagttacaac gttttctggt tgggacggga tgctggatgc aattttcaac agtcagtggt
     4561 ctgactgcga tcctgataaa attaatccag ggactcaggt caagggagat tgtgggagcc
     4621 cttctgttgg gatttcttat tttgtcagtt acatcctcat atcatggttg atcattgtta
     4681 acatgtacat tgtgttgatc atggagttct taagtattcc ttctcagaaa aaaagcagga
     4741 ccttgagtga agatgacttt aggagattct tccgggtgtg gaacaggttt gaccctgata
     4801 ggacccagta catagactct agcaagcttt ctgattttgc agctgctctg gatcctcctc
     4861 ttttcatggc aaaaccaaac aagggccagc ttgtggccat ggatctcccc atggctgcgg
     4921 gagacagaat ccactgcctc gacattttac ttgcctttac gaaaagagtg atgggaaagg
     4981 atgagagggt ggagaaaatc ctttcagaga tagaatccgg gttcatgtta gcgaaccctt
     5041 tcaaaatcac ttatgagccg attacaacta ctttgaaacg caaacaagag gcagtttcag
     5101 caaccatcat ccagcgtgca tataaaagct accgcttaag gcaaaatgac aagaatgtat
     5161 cagatactcc tgctatagat gaccgcagag atgatcttac ttctaaaggt gctcactctg
     5221 gcaaaatcga ggagaaggca tctattcaga cccagattta atgacacttc ccacttctac
     5281 tttctttaca tatgtcccca agcactaaat gttaactgat cttaagctgg agatcagaaa
     5341 ctagagataa tgataacatc tgtgtgccca gacatctcca tgacaagctc agctttaggg
     5401 tcagtcttct gatgcatcag aaaagacagc agctcagcgt tgctgcgggt agctgaacta
     5461 ggttactgtt gtctttttgg ctatttaaaa tgtttctact tggcttgtct agtgtggcct
     5521 ctagtggaag atgtgtagga ggaaaggaga agaaagggaa ataaaggtca gcagctatac
     5581 agttttcctt gtatatttta tatataattt tagaggcaac atgttgccac ccttgaggct
     5641 tagtggttaa tatgagataa ttccttaaag tgcaattgtg cttcagaaat aaaaaagccc
     5701 cgcattaaat cacaaacatg cataaacaaa tgctgggaat ggatgggaga ggaataatgc
     5761 aggtcttcag aaagtgcctt tcagttcagt cttgggctaa tttacaagat ctatttctta
     5821 aaacttttaa ctgaaacctc cccttccatt ttttgctttc ttcctgttgc ctttttttat
     5881 tttgtttcct tttttttttt ttgtttgctt gcttgcttgg ctttgttgct gttctttagt
     5941 tttggatagt agatgacatt gtttcattgc tcctggttcc tcctggcttg gcaagctctc
     6001 tgtgagctcc ccagcagctg agatttccag agcaggaagc atagaagaaa ccacccttaa
     6061 taatgagtac attttctgta tttgattcac atttatatct tttttaaagt gcacatttac
     6121 taaaaggata caataaagaa aaacgttaat ggactaaagc tttaccttat tctaaaaact
     6181 ggtgtatttt ttaccactta tccatagcag gtgcatgcta tcattaaatt agaagagtca
     6241 agggcaacca gcatgtttag ggattttctg tgccctgacc cttaatttgg aaactggcca
     6301 cttgaaaggt ctttccattg cactttgtat ttcttaaaat gtaaagaaga aagttatcct
     6361 tcaaacaata gggcttgtca attaacagat gaaagttaag ttggtatttt ataacaccat
     6421 tatatacaca ttagttttag tgttcactta tcttttcctt tctttgtttt ctatcttgtt
     6481 atacatctaa atactcatgc aaatgttttc tagttgagaa tctatgaaat aacagacatt
     6541 agttttaact actgacatta tttttgttaa tataaatagt tttaatttct tcacaaaaat
     6601 tgttgacttc cattcatgta gtttgagaaa tgatattttc ttcatttgaa taaaaaactg
     6661 atttcactgt gcctatgaag tcctccagaa ttaaaaaaga aaaaaatata tattctactg
     6721 acttagataa gaagcgttaa ataggtttta tgaaactcaa tgccattata tcctagagat
     6781 tgtattttgt aatgaacgca tggacttgag ccttaattcc tgtaatctac ttccaatctt
     6841 caaaactctg tgaccttacc aacttgcctt ggatgatcac ccaagtaaat gtaatttcag
     6901 aatctggtgg tttttctgga tgttgcttgt ttgtttgtta gtttggtttg tttggttggt
     6961 tggttggttg gtttttatta aagtatttcc ctttcactct tcactctctg tcatgtgatg
     7021 gagcctagag aagcatggga ggtctaactg gtgacatgat gacagcttct gtgtaaccgc
     7081 aatgtctcag ttttccaact tctgggaatg tcttagtgag gaaagtgcta cagatagtaa
     7141 gactgaatca taacttgctt ccataaatcc cattaaaaca acacactgag tttgtaaagt
     7201 attttgctgt tacctataaa gaatgacaag caatagccat ccaaaaagta gaatgtttca
     7261 tttctgaatg actgttacag aatatccttc ttctgagatc ccatagaaaa taataaatat
     7321 aacctgctaa gca
//
