LOCUS       NM_001253860            8287 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a),
            transcript variant 2, mRNA.
ACCESSION   NM_001253860
VERSION     NM_001253860.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Gomez-Del Arco,P., Isern,J., Jimenez-Carretero,D.,
            Lopez-Maderuelo,D., Pineiro-Sabaris,R., El Abdellaoui-Soussi,F.,
            Torroja,C., Vera-Pedrosa,M.L., Grima-Terren,M., Benguria,A.,
            Simon-Chica,A., Queiro-Palou,A., Dopazo,A., Sanchez-Cabo,F.,
            Jalife,J., de la Pompa,J.L., Filgueiras-Rama,D., Munoz-Canoves,P.
            and Redondo,J.M.
  TITLE     The G4 resolvase Dhx36 modulates cardiomyocyte differentiation and
            ventricular conduction system development
  JOURNAL   Nat Commun 15 (1), 8602 (2024)
   PUBMED   39366945
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Blair,G.A., Wu,X., Bain,C., Warren,M., Hoeker,G.S. and Poelzing,S.
  TITLE     Mannitol and hyponatremia regulate cardiac ventricular conduction
            in the context of sodium channel loss of function
  JOURNAL   Am J Physiol Heart Circ Physiol 326 (3), H724-H734 (2024)
   PUBMED   38214908
REFERENCE   3  (bases 1 to 8287)
  AUTHORS   Li,C., Zhang,Y., Shen,J., Bao,H., Zhao,Y., Li,D., Li,S., Liu,Y.,
            Yang,J., Zhou,Z., Gao,K., Zhao,L., Pei,Y., Lu,Y., Pan,Z. and Cai,B.
  TITLE     Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3
            of Structural, Metabolic, and Contractile Related Genes
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305992 (2024)
   PUBMED   38196272
REFERENCE   4  (bases 1 to 8287)
  AUTHORS   Ferrand,M.C., Giordano,G., Mougenot,N., Laporte,P.L., Vignier,N.,
            Leclerc,A., Algalarrondo,V., Extramiana,F., Charpentier,F. and
            Neyroud,N.
  TITLE     Intracardiac electrophysiology to characterize susceptibility to
            ventricular arrhythmias in murine models
  JOURNAL   Front Physiol 15, 1326663 (2024)
   PUBMED   38322613
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8287)
  AUTHORS   Qi,M., Ma,S., Liu,J., Liu,X., Wei,J., Lu,W.J., Zhang,S., Chang,Y.,
            Zhang,Y., Zhong,K., Yan,Y., Zhu,M., Song,Y., Chen,Y., Hao,G.,
            Wang,J., Wang,L., Lee,A.S., Chen,X., Wang,Y. and Lan,F.
  TITLE     In Vivo Base Editing of Scn5a Rescues Type 3 Long QT Syndrome in
            Mice
  JOURNAL   Circulation 149 (4), 317-329 (2024)
   PUBMED   37965733
  REMARK    GeneRIF: In Vivo Base Editing of Scn5a Rescues Type 3 Long QT
            Syndrome in Mice.
REFERENCE   6  (bases 1 to 8287)
  AUTHORS   Papadatos,G.A., Wallerstein,P.M., Head,C.E., Ratcliff,R.,
            Brady,P.A., Benndorf,K., Saumarez,R.C., Trezise,A.E., Huang,C.L.,
            Vandenberg,J.I., Colledge,W.H. and Grace,A.A.
  TITLE     Slowed conduction and ventricular tachycardia after targeted
            disruption of the cardiac sodium channel gene Scn5a
  JOURNAL   Proc Natl Acad Sci U S A 99 (9), 6210-6215 (2002)
   PUBMED   11972032
  REMARK    GeneRIF: Slowed conduction and ventricular tachycardia after
            targeted disruption of the cardiac sodium channel gene Scn5a
REFERENCE   7  (bases 1 to 8287)
  AUTHORS   Zimmer,T., Bollensdorff,C., Haufe,V., Birch-Hirschfeld,E. and
            Benndorf,K.
  TITLE     Mouse heart Na+ channels: primary structure and function of two
            isoforms and alternatively spliced variants
  JOURNAL   Am J Physiol Heart Circ Physiol 282 (3), H1007-H1017 (2002)
   PUBMED   11834499
REFERENCE   8  (bases 1 to 8287)
  AUTHORS   Nuyens,D., Stengl,M., Dugarmaa,S., Rossenbacker,T., Compernolle,V.,
            Rudy,Y., Smits,J.F., Flameng,W., Clancy,C.E., Moons,L., Vos,M.A.,
            Dewerchin,M., Benndorf,K., Collen,D., Carmeliet,E. and Carmeliet,P.
  TITLE     Abrupt rate accelerations or premature beats cause life-threatening
            arrhythmias in mice with long-QT3 syndrome
  JOURNAL   Nat Med 7 (9), 1021-1027 (2001)
   PUBMED   11533705
REFERENCE   9  (bases 1 to 8287)
  AUTHORS   Naylor,S.L., Carritt,B., Boileau,C., Beroud,C., Alexander,C.,
            Allderdice,P., Alimov,A., Ashworth,T., Bonifas,J., Bugert,P.,
            Buys,C.H., Chipperfield,M.A., Deng,G., Drabkin,H., Gemmill,R.M.,
            Grompe,M., Joensuu,T., Jonasdottir,A., Gizatullin,R., Krols,L.,
            Leach,R.J., Lott,S.T., Killary,A., Martinsson,T., Messiaen,L. et
            al.
  TITLE     Report of the sixth international workshop on human chromosome 3
            mapping 1995
  JOURNAL   Cytogenet Cell Genet 72 (4), 255-270 (1996)
   PUBMED   8641130
REFERENCE   10 (bases 1 to 8287)
  AUTHORS   Schleef,M., Werner,K., Satzger,U., Kaupmann,K. and Jockusch,H.
  TITLE     Chromosomal localization and genomic cloning of the mouse
            alpha-tropomyosin gene Tpm-1
  JOURNAL   Genomics 17 (2), 519-521 (1993)
   PUBMED   8406508
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147254.1, AC121922.3 and AK085822.1.
            
            Transcript Variant: This variant (2) switches an exon in the coding
            region, compared to variant 1. The resulting protein (isoform 2) is
            the same length as isoform 1 yet it has a different sequence.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-665               AK147254.1         169-833
            666-757             AC121922.3         30300-30391
            758-6883            AK147254.1         926-7051
            6884-8287           AK085822.1         886-2289
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 71.33 cM"
     gene            1..8287
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /note="sodium channel, voltage-gated, type V, alpha"
                     /db_xref="GeneID:20271"
                     /db_xref="MGI:MGI:98251"
     exon            1..327
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..6117
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sodium channel, voltage-gated, type V, alpha polypeptide;
                     sodium channel protein type 5 subunit alpha; sodium
                     channel protein type V subunit alpha; voltage-gated sodium
                     channel subunit alpha Nav1.5; sodium channel protein
                     cardiac muscle subunit alpha"
                     /codon_start=1
                     /product="sodium channel protein type 5 subunit alpha
                     isoform 2"
                     /protein_id="NP_001240789.1"
                     /db_xref="CCDS:CCDS57715.1"
                     /db_xref="GeneID:20271"
                     /db_xref="MGI:MGI:98251"
                     /translation="MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESRE
                     GLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTI
                     FRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYV
                     EYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSAL
                     RTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNL
                     RHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGT
                     CPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIF
                     FMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTI
                     RGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQ
                     LSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWP
                     LRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVL
                     DRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRK
                     CPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYN
                     MTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGL
                     SRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVV
                     GMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQ
                     SLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGL
                     RFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRF
                     EEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGG
                     HEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTAD
                     MTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRI
                     VEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWV
                     AYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRF
                     EGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNY
                     TIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRG
                     YEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQ
                     KKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVET
                     DDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTV
                     LSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLF
                     LVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNT
                     GPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEE
                     STEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMD
                     LPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLR
                     RKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNE
                     NFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDR
                     ESIV"
     exon            328..446
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            447..536
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            537..665
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..757
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            758..988
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1052
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1194
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1392
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1572
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1944
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2077
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2316
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2490
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2847
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3291
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3292..3453
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3574
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3575..3729
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3730..3903
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3904..4026
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4027..4308
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4309..4362
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4363..4500
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4501..4605
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4606..4876
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4877..8287
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8268..8273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /note="hexamer: AATAAA"
     polyA_site      8285
                     /gene="Scn5a"
                     /gene_synonym="mH1; Nav1.5; Nav1.5c; SkM1; SkM2"
                     /note="major polyA site"
ORIGIN      
        1 cttccttcca ggcagcctga ggagagcctg tgcccccaga agcaggatga gaagatggca
       61 aacttcctgt tacctcgggg caccagcagc ttccgtaggt tcacccggga gtcactggcg
      121 gccatcgaga agcgcatggc tgaaaaacaa gcccgtggtt cggccacctc acaggagagc
      181 cgtgagggcc tgccagagga ggaggctccc cggccccagc tggacctaca ggcctccaaa
      241 aagctgccag atctctatgg caacccaccc cgagagctca ttggggagcc cctggaagac
      301 ctggaccctt tctatagtac ccagaagacc ttcatcgtgc tgaataaggg caaaaccatc
      361 ttccggttca gtgccaccaa tgccttgtac gtcctcagcc ccttccaccc ggtgcgcaga
      421 gcggctgtga agattttggt acattcgctc ttcagcatgc tcatcatgtg caccatccta
      481 accaactgtg tgttcatggc acaacacgac cctccgcctt ggaccaaata tgttgagtac
      541 accttcaccg ccatctacac ctttgagtct ctggtcaaga ttctagctcg aggcttctgc
      601 ctgcatgcgt tcaccttcct ccgggacccg tggaactggc tagacttcag tgtgatcgtc
      661 atggcataca caactgaatt tgtggacctg ggcaatgtct cagccttacg caccttccga
      721 gtcctccggg ccctgaaaac tatatcggtc atttcaggcc tgaagacaat cgtgggagcc
      781 ctaatccagt ctgtgaagaa gctagccgat gtgatggtcc ttactgtctt ctgcctcagt
      841 gtctttgccc tcattggcct gcagctcttc atgggcaacc tgaggcacaa gtgcgtgcgt
      901 aactttactg agctcaatgg caccaatggt tccgtggagg ccgatggcat agtctggaac
      961 tctctggacg tctacctcaa tgacccagcc aattacctac tcaagaatgg caccacggat
     1021 gtgttgctgt gtgggaacag ctctgatgcc gggacatgtc ctgaaggcta ccggtgcctg
     1081 aaggcaggtg agaaccctga ccacggttac accagcttcg actccttcgc ctgggctttc
     1141 cttgcgctct tccgcctgat gacacaggac tgctgggaac gtctatacca gcagaccctg
     1201 aggtccgcag gaaagatcta catgatcttc ttcatgcttg tcatattcct gggctccttc
     1261 taccttgtga acttgatcct ggctgtggtg gccatggctt acgaggagca aaaccaagcc
     1321 accatcgccg agacggaaga gaaggagaag cgtttccagg aggccatgga gatgctcaag
     1381 aaggaacacg aggctctcac catcaggggc gtggatactg tgtcccgtag ctctctggag
     1441 atgtctcctt tggccccagt aaccaaccat gagagaagga gcaagaggag gaaacgactg
     1501 tcttcaggga cagaggatgg cggagatgac aggctcccca agtcggactc agaagacggt
     1561 cccagagcat tgaatcagct cagcctcacc catgggctca gccggacatc catgaggccc
     1621 cgctccagcc gagggagcat tttcacattc cggcgacggg accaaggctc cgaggcagat
     1681 tttgcagatg acgagaacag tacagcagga gagagcgaga gccaccgcac atcgctgctg
     1741 gtgccctggc ccctgcgccg tcccagcacc caagggcagc ccggctttgg aacctcagct
     1801 cctggtcatg tcctcaatgg caaaagaaac agcaccgtgg actgcaatgg ggtggtttcc
     1861 ttgctggggg caggggacgc agaggccacc tctccaggga gccaccttct ccgccctata
     1921 gtgctggaca gacccccaga cacgaccacg ccgtcagagg agccgggtgg gccccagatg
     1981 ctgacaccgc aggctccatg tgcagatggc tttgaggagc ccggggcacg gcaacgggca
     2041 ctcagcgccg tcagtgtcct caccagcgct ctggaagagt tggaggagtc ccaccgtaag
     2101 tgtccaccat gctggaatcg ctttgcccag cattacctca tctgggagtg ctgcccactc
     2161 tggatgtcca tcaagcagaa ggtgaagttc gtggtcatgg acccgtttgc tgacctcacc
     2221 atcaccatgt gcatcgtgct caacacgctc ttcatggctc tggagcacta caacatgaca
     2281 gccgagtttg aggagatgct gcaggtcgga aacttggtct tcacgggaat cttcacggca
     2341 gagatgacct tcaagatcat cgcccttgac ccatactact acttccagca gggctggaat
     2401 atcttcgaca gcatcatcgt catcctcagc ctcatggagc tgggcctgtc tcgcatgggc
     2461 aacctgtctg tgctgcgttc cttccgtctg ctgcgggtct tcaagctggc caaatcatgg
     2521 cccaccctga acacactcat caagatcatc gggaactctg ttggtgccct ggggaacctg
     2581 accctggtgc tggccatcat cgtcttcatc ttcgccgtgg tgggtatgca gctcttcggc
     2641 aagaactact cagagctgag gcaccgaatc agcgactctg gcctgctgcc ccgctggcac
     2701 atgatggact tcttccacgc cttcctcatc atcttccgca tcctctgtgg agagtggatc
     2761 gagaccatgt gggactgcat ggaggtgtct gggcagtcgc tgtgcttgct ggtcttcctg
     2821 ctggtcatgg tcattggcaa ccttgtggtc ctgaatctct tcttggcctt gctgctcagc
     2881 tccttcagcg cagacaacct cacagcccct gacgaggatg gggagatgaa caacctccag
     2941 ctggccctgg ctcgcatcca aaggggcctt cgctttgtca agcggaccac ctgggacttc
     3001 tgctgcgggc tcctgcggcg gcgacctaag aagcccgcgg ctcttgccac ccacagccag
     3061 ctgcccagct gtatcgccgc tcccaggtcc ccaccacccc cagaggtgga gaaggcgccc
     3121 ccagcccgca aggaaacacg gttcgaggaa gacaagcggc cgggccaggg cacccctggg
     3181 gataccgagc ctgtgtgtgt tcccatcgca gtggctgagt cagacactga tgaccaggaa
     3241 gaggatgagg agaacagcct tggcacggag gaggaagagt ccagcaaaca gcaggaatcc
     3301 caagttgtgt ctggtggcca cgagcccccc caggagccca gggcctggag ccaggtgtca
     3361 gagaccacat cctctgaagc cgaggccagt acgtctcagg cagactggca gcaagagcgg
     3421 gaagcggagc cccgggcccc ggggtgcggt gagactcccg aggacagtta ctccgagggt
     3481 agcaccgctg acatgaccaa caccgcagac ctcctggagc aaatcccaga cctcggggag
     3541 gacgtcaagg acccagagga ctgcttcact gaaggctgcg tccgacgttg tccttgctgc
     3601 atggtggaca cgacgcaggc cccagggaag gtctggtgga gactgcgcaa gacctgctac
     3661 cgcatagtgg agcacagctg gttcgagact ttcatcatct tcatgatcct gctcagcagc
     3721 ggagcgctgg ccttcgagga catctacctg gaggagcgga agaccatcaa ggttctgctg
     3781 gagtacgccg acaagatgtt cacctatgtc tttgtgttgg agatgctgct caagtgggtg
     3841 gcctacggct tcaagaagta cttcaccaac gcctggtgct ggctggactt cctaattgtg
     3901 gacgtctcac tggtcagcct cgtggccaac accttaggct ttgccgagat gggtcccatc
     3961 aagtccctga ggacactgcg tgcacttcga cccctgaggg ctttgtcacg attcgagggc
     4021 atgagggtgg tggtcaacgc gctggtgggc gccatcccct ccatcatgaa tgtcctcctc
     4081 gtctgcctca tcttctggct catcttcagc atcatgggcg tgaacctctt cgccgggaag
     4141 ttcggtaggt gcatcaacca gaccgaaggg gacctgcctc tgaactacac cattgtgaac
     4201 aacaagagcg agtgtgagtc cttcaacgtg actggagagt tgtactggac caaggtgaaa
     4261 gtcaactttg acaacgtggg ggctgggtac ctggccctgc tgcaggtggc gacatttaaa
     4321 ggctggatgg acatcatgta tgcggctgtg gactccagag ggtatgaaga gcagccgcag
     4381 tgggaggaca acctctacat gtacatctac ttcgtcgtct tcatcatctt cggctccttc
     4441 ttcaccctca acctgtttat tggtgtcatc attgataact tcaaccagca gaagaaaaag
     4501 ttagggggcc aggacatctt catgacggag gagcagaaga agtactacaa tgccatgaag
     4561 aagctgggct ccaagaaacc ccagaagccc atcccacggc ctctgaacaa gtaccagggt
     4621 ttcatattcg acattgtgac caagcaggcc ttcgatgtta ccatcatgtt cctcatctgt
     4681 ttaaacatgg tgaccatgat ggtagagaca gatgatcaga gccctgaaaa agtcaacatc
     4741 ttggccaaga tcaacctgct cttcgtggcc atcttcacag gagagtgtat tgtcaagatg
     4801 gctgccctgc gccactatta cttcaccaac agctggaaca tcttcgattt cgtggttgtc
     4861 atcctctcca tcgttggcac tgtcctctcc gacatcatcc agaagtactt cttctcccca
     4921 acgctcttcc gtgtcatccg tctggccagg atcggccgca tcctcaggct gatccgagga
     4981 gccaagggga ttcgcacgtt gctcttcgcc ctcatgatgt ccctgcccgc cctcttcaac
     5041 atcggcctcc tcctcttcct cgtcatgttc atctactcca tcttcggcat ggccaacttt
     5101 gcctacgtca agtgggaggc tggcatcgac gacatgttca acttccagac ctttgccaac
     5161 agcatgctgt gccttttcca gatcaccacg tcggctggct gggatggcct cctcagcccc
     5221 atcctcaaca caggaccccc ctactgtgac cccaacctgc ccaacagcaa tggctccagg
     5281 gggaactgtg ggagcccagc agtgggcatc ctcttcttca ccacctacat catcatctcc
     5341 ttcctcatcg tggtcaacat gtacattgcc atcatcctgg agaacttcag tgtggccaca
     5401 gaagagagca cagagcccct gagcgaggat gacttcgaca tgttctatga gatctgggag
     5461 aagttcgacc cggaggccac ccagttcatt gagtatttgg ccctgtccga ctttgccgat
     5521 gccctgtctg agccactccg catcgccaag cccaaccaga taagcctcat caatatggac
     5581 ctgcccatgg tgagcgggga ccgcatccac tgtatggaca tcctgttcgc cttcaccaag
     5641 agggtgctcg gggagtctgg ggagatggat gccctgaaga tccagatgga ggagaaattc
     5701 atggcggcca atccttccaa gatctcctat gagcccatca ccaccaccct gcggcgaaaa
     5761 catgaggagg tgtcggccac ggtcatccag cgggccttcc ggaggcacct cctgcagcgc
     5821 tcggtgaagc atgcttcctt cctcttccgc cagcaagcgg gcagcagtgg cctctctgat
     5881 gaggatgccc ccgagcgaga gggactcatt gcctacatga tgaatgagaa cttctcccgg
     5941 cgcagcggtc ccctctccag ctcttccatc tcctccacgt ccttcccacc gtcctatgac
     6001 agcgtcacga gggccaccag tgataacctc ccagtgcgtg cgtcggacta cagccgcagc
     6061 gaagatcttg cagacttccc tccatctcca gatagggacc gagagtctat agtgtgaacc
     6121 tggccacccg tccggccagg acgtgccctg ttgtatcctg acaaacctta agcagttgaa
     6181 accagtgtcg gctaaggcct tcctagctct ggaggtgaca ggggaacctc caggtggagg
     6241 aaccacacgg cagagttctg tgtgtctgtg ttgaagctag aggcggctag agcaaccctg
     6301 gctggtagag gcctggcaca ccctagtagc cgggtctggc caggcagcgc cgcatggctc
     6361 tgaacagcaa ctctgtcccc ctctgaggtg aaacgggaaa cacactgtac atgttgtgaa
     6421 caagctttca taggtttatt atatttgata tttttttact cgagcaaaga acatcttttt
     6481 ccatgaacat ctgcagcagt tcacacggcc tcttcctaac cctgagcagg tgtcaatgga
     6541 gaactctgct ctcagagcta acgtggaatc ttcgcaccca aggggagggt tgtggttcct
     6601 gtcacctggg ctgaggagcc aggaaggcct aagcacttca ctcacacacg cgagtgggcg
     6661 agtgcgtgtg cctgtgcaca cttcacacac tggggcggga cataggccgc agggacccag
     6721 ggtcgcctca ggcaaatgtg gttggtcggt ggctccaaag gcgagaggtt tttcttcctg
     6781 gccccccagt gtgggtttct tcctgtgaac tcactctgtg cccctgttgt ccacaggatt
     6841 ccagtctcct gttctcagtc aggcttcgct tgaggtcagt gctataaaaa ttgaaagctt
     6901 ccagagagct gccctggtct cccttcctct cctgtaagag tattaatgtt ggggcccaag
     6961 ggctcgagga aggcagcctc gctcacagcc aggcacgtag cttgcttctg agatgtggca
     7021 ggccctgtcc caaggccaag ggaggagaag gctgtcccac gtgggccgct gaactgtgtc
     7081 cagctgtggc agcaaaggga gcggtctcca tttcccaggc tcctctgggg tctctgcctc
     7141 tggccgtgtg tcaaggagat gcccactggg gctccaaccg tcttcagaca ctgtggaagt
     7201 gggagggtag cctggccctg tctaggagaa aggccctgcg tggtcaggcc tggttctgtg
     7261 acctggaagg tgacaagcca tccatccccc atgggagagg agacagtgtg gcatctgggc
     7321 ccagcccagg gtctgactat cctaggcgtc ttctgagaag gctttttcag ggaaaacaaa
     7381 caaaccgatt ttataagtcc aatcatcccc gtgacctcct cagctgctga caatcctatt
     7441 tagcatatgc aaatctttta ttgtagagga ccagaccatc gctgctgaag ggacaggctg
     7501 gtggagcctg agcaggcagg ggcttggctg cccaagtcta gctctccagc agagccaccc
     7561 tcggctgggg gcacccacct cacaggccac ctcagtggcg cctgcccact gcgggctggc
     7621 tgctcctacc tacctcgccg agctgtcgga gggctggacg ctcctggcag tgctgaagga
     7681 gggggcactg cgggcaagtc aagtattatg tttcttcttg tcacctcagt tcccttggtg
     7741 gcagctggct cccgccccac ctgcccctgg cggcttatcc aatgtcccca cggagtccgg
     7801 tgtggggggt ttggttctaa ctgtcctggt gatgcctctc tgagtggaaa tcttattttt
     7861 gtagatctgt actttgctct cgaggcttgg agagatgtgt gtccctccct ggcactcact
     7921 tcaggccgca tgggcaggaa tgtggcctgg ggctggcagg gttggcaggg ctgccatccc
     7981 agctggccgg aaagaggctg tggtttcgaa tatgcagatg gccttggagc cttaagacag
     8041 gtgccgggct ggctgcagac agggtggctg aggggagggg caatcgagtc ccgagtcctt
     8101 ggcttagcat ggcctccttt tctagaactg cacagagggg aggagagagt agccggtaga
     8161 cggaaggagg gaggggcatg cgtacatgtc aggtgttgtt tgaggtaaat gagcacttct
     8221 cactaagctt cgtgtataaa tggaatacat tatttttcaa acaaacgaat aaatggctta
     8281 catgacc
//
