LOCUS       NM_133199               8224 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a),
            transcript variant 1, mRNA.
ACCESSION   NM_133199 XM_036156229
VERSION     NM_133199.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   DiFranco,M. and Cannon,S.C.
  TITLE     Voltage-dependent Ca2+ release is impaired in hypokalemic periodic
            paralysis caused by CaV1.1-R528H but not by NaV1.4-R669H
  JOURNAL   Am J Physiol Cell Physiol 323 (2), C478-C485 (2022)
   PUBMED   35759432
  REMARK    GeneRIF: Voltage-dependent Ca(2+) release is impaired in
            hypokalemic periodic paralysis caused by CaV1.1-R528H but not by
            NaV1.4-R669H.
REFERENCE   3  (bases 1 to 8224)
  AUTHORS   Corrochano,S., Blanco,G., Williams,D., Wettstein,J., Simon,M.,
            Kumar,S., Moir,L., Agnew,T., Stewart,M., Landman,A., Kotiadis,V.N.,
            Duchen,M.R., Wackerhage,H., Rubinsztein,D.C., Brown,S.D.M. and
            Acevedo-Arozena,A.
  TITLE     A genetic modifier suggests that endurance exercise exacerbates
            Huntington's disease
  JOURNAL   Hum Mol Genet 27 (10), 1723-1731 (2018)
   PUBMED   29509900
  REMARK    GeneRIF: we performed an unbiased mutagenesis screen on an HD mouse
            model, identifying a mutation in the skeletal muscle voltage-gated
            sodium channel (Scn4a, termed 'draggen' mutation) as a novel
            disease enhancer
REFERENCE   4  (bases 1 to 8224)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 8224)
  AUTHORS   Wu,F., Mi,W., Fu,Y., Struyk,A. and Cannon,S.C.
  TITLE     Mice with an NaV1.4 sodium channel null allele have latent
            myasthenia, without susceptibility to periodic paralysis
  JOURNAL   Brain 139 (Pt 6), 1688-1699 (2016)
   PUBMED   27048647
  REMARK    GeneRIF: NaV1.4 null mice have latent myasthenia and a right shift
            of the force-stimulus relation, without evidence of periodic
            paralysis. Sodium current density was half that of wild-type muscle
            and no compensation by retained expression of the foetal NaV1.5
            isoform was detected. Mice null for NaV1.4 did not survive beyond
            the second postnatal day.
REFERENCE   6  (bases 1 to 8224)
  AUTHORS   Zimmer,T., Bollensdorff,C., Haufe,V., Birch-Hirschfeld,E. and
            Benndorf,K.
  TITLE     Mouse heart Na+ channels: primary structure and function of two
            isoforms and alternatively spliced variants
  JOURNAL   Am J Physiol Heart Circ Physiol 282 (3), H1007-H1017 (2002)
   PUBMED   11834499
REFERENCE   7  (bases 1 to 8224)
  AUTHORS   Crawshaw,L.I., Wallace,H.L., Christensen,R. and Crabbe,J.C.
  TITLE     Influence of ethanol on thermoregulation: mapping quantitative
            trait loci
  JOURNAL   Physiol Genomics 7 (2), 159-169 (2001)
   PUBMED   11773602
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 8224)
  AUTHORS   Ribaux,P., Bleicher,F., Couble,M.L., Amsellem,J., Cohen,S.A.,
            Berthier,C. and Blaineau,S.
  TITLE     Voltage-gated sodium channel (SkM1) content in dystrophin-deficient
            muscle
  JOURNAL   Pflugers Arch 441 (6), 746-755 (2001)
   PUBMED   11316257
REFERENCE   9  (bases 1 to 8224)
  AUTHORS   Ptacek,L.J., Tawil,R., Griggs,R.C., Meola,G., McManis,P.,
            Barohn,R.J., Mendell,J.R., Harris,C., Spitzer,R., Santiago,F. et
            al.
  TITLE     Sodium channel mutations in acetazolamide-responsive myotonia
            congenita, paramyotonia congenita, and hyperkalemic periodic
            paralysis
  JOURNAL   Neurology 44 (8), 1500-1503 (1994)
   PUBMED   8058156
REFERENCE   10 (bases 1 to 8224)
  AUTHORS   Ambrose,C., Cheng,S., Fontaine,B., Nadeau,J.H., MacDonald,M. and
            Gusella,J.F.
  TITLE     The alpha-subunit of the skeletal muscle sodium channel is encoded
            proximal to Tk-1 on mouse chromosome 11
  JOURNAL   Mamm Genome 3 (3), 151-155 (1992)
   PUBMED   1352160
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL604045.7.
            
            On or before Apr 1, 2022 this sequence version replaced
            XM_036156229.1, NM_133199.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.14798.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AL604045.7         147524-147608       c
            86-180              AL604045.7         137171-137265       c
            181-855             AL604045.7         131166-131840       c
            856-974             AL604045.7         130949-131067       c
            975-1064            AL604045.7         130752-130841       c
            1065-1193           AL604045.7         130321-130449       c
            1194-1285           AL604045.7         129806-129897       c
            1286-1600           AL604045.7         127464-127778       c
            1601-1664           AL604045.7         126263-126326       c
            1665-1806           AL604045.7         125941-126082       c
            1807-2016           AL604045.7         124360-124569       c
            2017-2170           AL604045.7         123471-123624       c
            2171-2409           AL604045.7         121203-121441       c
            2410-2583           AL604045.7         119109-119282       c
            2584-2940           AL604045.7         117424-117780       c
            2941-3417           AL604045.7         112046-112522       c
            3418-3553           AL604045.7         110069-110204       c
            3554-3708           AL604045.7         109560-109714       c
            3709-3882           AL604045.7         108755-108928       c
            3883-4005           AL604045.7         108180-108302       c
            4006-4284           AL604045.7         106496-106774       c
            4285-4338           AL604045.7         106257-106310       c
            4339-4476           AL604045.7         105925-106062       c
            4477-4581           AL604045.7         104837-104941       c
            4582-4852           AL604045.7         103957-104227       c
            4853-8224           AL604045.7         99597-102968        c
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 68.91 cM"
     gene            1..8224
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /note="sodium channel, voltage-gated, type IV, alpha"
                     /db_xref="GeneID:110880"
                     /db_xref="MGI:MGI:98250"
     exon            1..85
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            86..180
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            181..855
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             583..6108
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /note="sodium channel alpha-subunit; sodium channel
                     protein type IV subunit alpha; voltage-gated sodium
                     channel subunit alpha Nav1.4; sodium channel protein
                     skeletal muscle subunit alpha; sodium channel,
                     voltage-gated, type IV, alpha polypeptide"
                     /codon_start=1
                     /product="sodium channel protein type 4 subunit alpha"
                     /protein_id="NP_573462.2"
                     /db_xref="CCDS:CCDS48961.1"
                     /db_xref="GeneID:110880"
                     /db_xref="MGI:MGI:98250"
                     /translation="MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQ
                     MEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAI
                     FRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDV
                     EYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISAL
                     RTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNL
                     RQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEA
                     YINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLA
                     LFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEA
                     TLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTS
                     GEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIIL
                     LIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLI
                     AMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNM
                     LIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHD
                     FFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSS
                     FSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPG
                     GAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYL
                     TIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPE
                     EQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETF
                     IVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFT
                     NAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNA
                     LLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECE
                     SLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVN
                     LYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKL
                     GSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDI
                     LYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFV
                     SPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFG
                     MSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLE
                     NPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDD
                     FEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKI
                     HCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAI
                     KIQRAYRRHLLQRSVKQASYMYRHSQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQ
                     SQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSPPPPSSPPQGQTVRPGVKESLV"
     exon            856..974
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1064
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1193
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1285
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1600
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1664
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1806
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..2016
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2170
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2409
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2583
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2584..2940
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3417
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3553
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3708
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..3882
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..4005
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4006..4284
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4338
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4339..4476
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4477..4581
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4582..4852
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     exon            4853..8224
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8206..8211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /note="hexamer: AATAAA"
     polyA_site      8224
                     /gene="Scn4a"
                     /gene_synonym="mH2; Nav1.4; SkM1"
                     /note="major polyA site"
ORIGIN      
        1 cccccagagc tccagcccca gcctgaggtg ggcacggcac ccggggctct cgtctgccac
       61 cgctcttctc tgcttctggc caacaatgca tgggaagggc aggcagaagg ggctcactcc
      121 tgggcggcag tgactccatt tgacttccca gccctgggag ggattctcca aggctacaag
      181 gttgaagtca cacagttcct ggcatctgaa cccaggagac agttcaggac agtggggcat
      241 tggccccaag ttcctaaggc tggagaagat ctggtttgct ttctgttgat cctgaagctg
      301 gcacaaccta ttgctgagaa gagaacacag tggggcctgg ggtgggggga tgctccgtgc
      361 ccagtgtcct gtgcatcagc acactcaggt cttgcaggca catctctcag ctccaacaag
      421 accaggacac ccacctccct gacccaactc ctgggcaggc agagagtgag gcatacacag
      481 tagacccagg gcgttagtga ccacccaaaa cccaaggact gcgggctgta gctgctccgt
      541 ctttgcctac caccctctgg cccctgagcc cagaatgcaa ggatggccag ctcatctctg
      601 cccaccctgg tcccccctgg tccccactgc ctgcgcccct tcaccccaga gtccctggca
      661 gccatagaac agcgggcaat ggaggaggaa gcccggctgc agcggaacaa gcaaatggag
      721 attgaagagc ctgagcggaa gccacgcagt gacctggaag ctggcaagaa cctcccactc
      781 atctatgggg accccccacc tgaagtcatt ggcgtccccc tggaggacct ggacccttac
      841 tacagtgaca agaagacctt cattgtgctc aacaaaggaa aggccatctt ccgattctct
      901 gccacccctg ccctctacat gctgagcccc ttcagcatcg tccggcgggt ggctatcaag
      961 gtgctcatcc acgcgctgtt cagcatgttc atcatgatca ccatcttgac caactgcgtg
     1021 ttcatgacca tgagcaaccc gccttcttgg tccaaggacg tggagtacac cttcacaggg
     1081 atctacacct ttgagtccct cattaagatg ctggcccgag gcttttgcat tgatgacttc
     1141 acattcctcc gagacccctg gaactggctg gacttcagtg tcatcacaat ggcgtacgtg
     1201 acagagtttg tggacttggg caacatctca gccctgagga ccttccgtgt gctgcgggcc
     1261 ctgaaaacca tcacggtcat cccagggctg aagacgatcg tgggagccct gatccagtct
     1321 gtgaaaaagc tctcggatgt gatgatcctc actgtcttct gcctgagcgt ctttgccctg
     1381 gtggggctgc agcttttcat gggaaacctg cgtcagaagt gcgtgcgttg gcccccgccc
     1441 atgaatgaca ccaacaccac gtggtatggt aatgatacgt ggtacggcaa tgacacctgg
     1501 tacggcaatg acacctggta tggcaatgac acttggaaca gccaggagag ctgggtcagc
     1561 aactctacct ttgactggga ggcctacatc aatgacgaag ggaacttcta tttcttggag
     1621 ggctccaatg atgccctgct ctgtgggaat agcagtgatg ccgggcactg ccctgagggc
     1681 tacgaatgca tgaaggctgg gaggaacccc aactatggct acaccagcta cgacaccttc
     1741 agctgggcct tcctggctct cttccgtctc atgacgcagg actactggga gaaccttttc
     1801 cagctgaccc ttcgagccgc tggcaagacc tacatgatct tctttgtggt catcatcttc
     1861 ctgggttcct tctacctcat caatctgatc ctggcggtgg tggccatggc atatgctgag
     1921 cagaatgagg ctaccctggc cgaagaccag gagaaagagg aggagttcca acagatgctt
     1981 gaaaaattca aaaaacatca ggaggaactg gaaaaggcga aggctgccca ggctctggaa
     2041 ggtggagagg aggcagatgg ggacccaacc cacagcaaag actgcaatgg tagcctggat
     2101 acatccgggg agaaggggcc cccaaggcca agctgcagcg cagagagtgc catctcagat
     2161 gctatggagg agctggaaga ggcccatcag aagtgccccc catggtggta caagtgtgca
     2221 cacaaagtgc tcatctggaa ctgctgtgcc ccgtgggtga agttcaagca tattatcctc
     2281 ctgattgtca tggacccctt tgtggacctg ggcatcacca tctgcatcgt gctcaacacc
     2341 ctcttcatgg ccatggagca ctaccccatg actgagcact ttgacaacgt gctctctgtg
     2401 ggcaacttgg tcttcacggg catcttcact gcagagatgg tgctgaagtt gattgctatg
     2461 gacccctacg aatatttcca acagggctgg aacatcttcg acagcttcat cgtcaccctc
     2521 agcctggtgg agctgggcct ggccaacgtg cagggcctat cagtgctccg ttctttccgt
     2581 ctgctgcgtg tcttcaagct ggccaagtca tggccaacac tcaacatgct catcaaaatc
     2641 attggcaact cagtgggtgc gctgggcaac ctgaccctgg tgttggccat catcgtcttc
     2701 atcttcgccg tggtgggcat gcagctgttt ggcaagagct ataaggaatg cgtgtgcaag
     2761 attgcctcag actgcagcct gccccgctgg cacatgcacg atttcttcca ctccttcctc
     2821 atcgtcttcc gcatcctctg tggggagtgg atcgagacca tgtgggactg catggaagtg
     2881 gccggccagg ccatgtgcct caccgtcttc ctcatggtca tggtcattgg caacctggtg
     2941 gtcctgaatc tgttcctggc tctcctgttg agttccttca gcgctgacag tctggcagcg
     3001 tcagatgaag atggtgagat gaacaaccta cagatcgcca tcgggcgtat caagtggggc
     3061 atcgcctttg ccaaaacctt cctcttgggg ctgttgcatg gcaagatcct gagcctcaaa
     3121 gacataatgc tcagtctcgg ggagcctggg ggtgctgggg aaaacgggga gagccctccc
     3181 gaggatgaga agaaggaacc acctccggaa gatgggaaca aggagctgaa agacaatcat
     3241 atcctgaacc acgtgggcct gactgatgga ccccgctcca gcatcgagat ggaccacctt
     3301 aacttcatca acaaccccta cctcactatc catgtgccca ttgcctccga ggagtccgac
     3361 ttggaaatgc ccacagagga ggagactgac accttctcgg agcctgagga catcaagaag
     3421 ccactacagc ctctgtacga cgggaactcc tccgtctgca gcacagctga ctacaagccc
     3481 cccgaagagg accctgagga gcaggctgag gagaaccctg agggggagct acctgaggag
     3541 tgtttcacag aggcctgtgt gaagcgctgc ccctgcctct atgtggacat ctcccagggc
     3601 cgcgggaaaa tgtggtggac actccgcagg gcctgcttca agattgttga gcacaactgg
     3661 tttgagacct tcatcgtctt catgatcttg ctcagcagcg gggccctggc cttcgaggac
     3721 atctacattg aacagcggcg agtcatccaa accatcctgg aatatgctga caaggtcttc
     3781 acctacatct tcatcctgga gatgctgcta aagtgggtgg cctacggctt caaggtgtac
     3841 ttcaccaacg cctggtgctg gctcgacttc ctcattgtgg atgtctccat catcagtctg
     3901 gtggctaact ggctgggcta ctctgagctg gggcccatca aatccctgcg cacgctccgg
     3961 gccctgcgac ctctgagggc actgtcccga tttgaaggca tgagggtggt ggtgaacgcc
     4021 cttctgggag ccatcccttc catcatgaac gtgctccttg tctgcctcat cttctggctc
     4081 atcttcagca tcatgggggt caacttgttt gctggaaagt tctactactg catcaacaca
     4141 accacctccg agagattcga catctctgtg gtcaacaaca agtccgagtg tgagagcctc
     4201 atgtacaccg gccaggtccg ctggatgaac gtcaaggtca actatgacaa cgtgggtctc
     4261 ggctacctct ctcttcttca ggtggccact ttcaagggtt ggatggacat catgtatgca
     4321 gctgtggact cccgggagaa agaggagcag ccagactacg aggtgaacct ctacatgtac
     4381 ctttacttcg tcatctttat catcttcggc tccttcttca ccctcaatct cttcattggc
     4441 gtcatcattg acaacttcaa ccaacagaag aagaagtttg gagggaaaga catcttcatg
     4501 acagaggaac agaagaaata ctacaatgcg atgaagaagc ttggctccaa gaagccccag
     4561 aagccaatcc cccggcctca gaacaagatc caaggcatgg tgtacgactt cgtgacaaag
     4621 caggtgtttg acatctctat catgatcctc atctgcctca acatggtgac gatgatggtg
     4681 gagacggatg accagagcca gctcaaggtg gacatcctgt ataacatcaa catggtcttc
     4741 attatcgtct tcacagggga gtgtgtgctc aagatgtttg ccctgcgcca ttactacttc
     4801 accattggct ggaatatctt cgacttcgtg gttgtcatct tgtccattgt gggtctcgca
     4861 ctctctgact tgatacagaa atactttgtg tcacccacac tgttccgtgt gatccgattg
     4921 gctcggatcg ggcgtgtctt gcgtctgatc cgtggggcca agggcatccg gacgctgctg
     4981 ttcgccctga tgatgtccct gcccgccctc ttcaacatcg gcctccttct ctttctggtc
     5041 atgttcattt actccatctt cggcatgtct aacttcgcct acgtcaagaa agagtcgggc
     5101 atcgacgaca tgttcaactt tgagaccttt ggcaacagta tcatctgcct cttcgagatc
     5161 accacgtcag ctggctggga cgggcttctg aaccccatcc tcaacagtgg gcccccagac
     5221 tgtgacccga cattggagaa cccaggcacc aatatcaagg gtgactgcgg caacccatcc
     5281 atcggcatct gtttcttctg cagctacatc atcatctcct tcctcatcgt ggtcaacatg
     5341 tacattgcca tcatcctaga gaatttcaat gtggccactg aagagagcag tgagcccctc
     5401 tgtgaggatg acttcgagat gttctatgag acctgggaga agtttgaccc cgatgcaact
     5461 cagttcatcg actacagccg cctttctgac tttgtggaca ccctgcagga gcccttgaaa
     5521 attgccaagc ccaataagat caagcttatc acattagacc tgcccatggt acccggggac
     5581 aagatccact gcctggacat cctctttgcc ctgaccaaag aggtactggg tgactctggg
     5641 gaaatggatg ctctcaaaca gaccatggag gagaagttca tggcagctaa cccctccaag
     5701 gtctcctatg agcccatcac caccaccctc aagaggaagc aggaggaggt gtgtgctatc
     5761 aaaatccaga gggcttaccg ccgccatctg ctgcagcgct ctgtgaagca ggcctcttac
     5821 atgtaccgcc acagccagga aggcaatggc gatggggccc ctgagaagga gggattgctt
     5881 gccaacacca tgaacaagat gtatggctct gagaaagagg acaacggtgt gcagagccag
     5941 ggggagaaag agaaagactc aacagaggat gctggaccca ccacggaggt cacagccccc
     6001 agcagctcgg acactgccct gactcctcct cctccttctc ctcctccacc atcatcacca
     6061 ccacaagggc agacagtgcg cccaggggtc aaagagtctc ttgtctagag agcagcatca
     6121 aggctgcatg cagagttggg gaacggggct ttgagatgga ggagggtggg gctctacagg
     6181 gcttgacacc tgggctgagg gaaatggaga ttgtgctcag ggtcaggtgg ctctgaagat
     6241 cccgcctcct gatttaatct ttacgatgct ctaacattct ctcggccagg gggaatactg
     6301 ttggtatgaa ggggttggtc accttcctgt cactacttta agagcccagg tcccccagct
     6361 ctagccagga caaagacaaa ctttaacccc caaacttaca ctgaccctag agcttagcta
     6421 ctcctctcac ccccatgatt ctgggacaca gctcccccca cccctcgccg gctcaggaat
     6481 gccctgaaaa ggggaaccgg ttctggtaca gaaattgctg caccaagtca attggccgct
     6541 ggatccggat gtatcaggct ggaaacatga cttctgaaac ctttcgccca gtcatatcca
     6601 ggccggtgga cagctcccct ggctggcccc tgggggaaga actgtggaag gattgtcctg
     6661 aggaatctct agattgcatg gatgggtcac acaggctggg tcctgccagc tgcattcatg
     6721 cacttgagcc tgagcaccca catctgcctg aggtcaggtt ggaagctctc taataagact
     6781 ctgtctgttc cctcctcctt gtctccacgc cttcttcctt tgaccgccag ggctcatggg
     6841 agccccatca cgcccgagag acctccttct tcctgtttaa tctctgccca tctcttccct
     6901 gtcccttctc tgcttcctca agtgactctg agaatgttag aactcagcac acggggagat
     6961 atgttgatgc tggggcgtgc agtaagaagg ctcgctagac tcggtctcag tcttcctgct
     7021 acctggaaag ccacttttcc ccctgagaca gggtttcttt tctctgtgta tccctggtca
     7081 tcctggaatt cattctgtag atctggctag cctcaaactc agggatccgc ccacctctgc
     7141 ctcccacgtg ctgaaattaa aggcttgcgc caccactgcc aagaggaaag ccaatgtgct
     7201 tagcgctgct caccaggatc tgacttcgcc aggctcagag agcagagacc agacttggaa
     7261 gaggggatga gggtcctgct caggggtctc tggattcagc agtcacttgt atgtgtgcct
     7321 gcacatctgt gcttgtgagc ctgagctcat cctgagtttg tgcacgactg tatgtgtgtg
     7381 agccactgtg tctgcagata tgcatgtgtc tgtgaatgtt tgtgtgtgtg tgtgtgtgtg
     7441 tgtgtgtgtg agagagagag agagagagag agagagagag agagagagag agaatataaa
     7501 cattttgcct tctccaccca tttgttcatc taagcatccc taaatgtggg aaagggcatt
     7561 ttcctgtgcg ggagtaaaca cggaggaaca catatctgag ctttgagtcg ccttctggcc
     7621 taaattcctt ttctttttct tcttgtttct cataaacagt ttgggtaaaa ctcaggaagt
     7681 ggtaaaaaca agatgtgtgc gcacgtgcat tcacatatgt gggcgccatg ggtctccttg
     7741 cttaccctgc tcgctcaggc ctccctgcct cacctggatg tccctctggc tccctcttct
     7801 gagattctgt ctcccccttt ctcagtttct cctcaacacc catggcctcg ccccccttct
     7861 ccttcccatg tctcttgtgt agaaggagtt gagtcctcct tcctgtggta ctgactgtcc
     7921 cctcagaagg tccctgcagt gggagtcagt agtcccttgc ccagaaccca gctactctgt
     7981 ctttcccaca ctggcccagc ctggcttggc cctcagagcc tcaggaatct ataaggtctg
     8041 atgttctcca ccaactgtaa gagtcactgt ggaagaagat ctgtgagcag agcctgctgt
     8101 cgctggcggt gaaccctctg ctgagtgagc ccagctccca ccttgtctag gtgggaaaca
     8161 gcctgactcc aggctgttgg ataagcttct ttgtacagtt cttataataa accctcaccc
     8221 ggtg
//
