LOCUS       NM_001355167            9656 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus sodium channel, voltage-gated, type III, alpha
            (Scn3a), transcript variant 2, mRNA.
ACCESSION   NM_001355167 XM_011239392
VERSION     NM_001355167.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Wang,Y., Zhang,Y., Ma,N., Zhao,W., Ren,X., Sun,Y., Zang,W. and
            Cao,J.
  TITLE     SIRT1 mediates the excitability of spinal CaMKIIalpha-positive
            neurons and participates in neuropathic pain by controlling Nav1.3
  JOURNAL   CNS Neurosci Ther 30 (6), e14764 (2024)
   PUBMED   38828629
  REMARK    GeneRIF: SIRT1 mediates the excitability of spinal
            CaMKIIalpha-positive neurons and participates in neuropathic pain
            by controlling Nav1.3.
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Heighway,J., Sedo,A., Garg,A., Eldershaw,L., Perreau,V.,
            Berecki,G., Reid,C.A., Petrou,S. and Maljevic,S.
  TITLE     Sodium channel expression and transcript variation in the
            developing brain of human, Rhesus monkey, and mouse
  JOURNAL   Neurobiol Dis 164, 105622 (2022)
   PUBMED   35031483
REFERENCE   3  (bases 1 to 9656)
  AUTHORS   Martinez-Espinosa,P.L., Yang,C., Xia,X.M. and Lingle,C.J.
  TITLE     Nav1.3 and FGF14 are primary determinants of the TTX-sensitive
            sodium current in mouse adrenal chromaffin cells
  JOURNAL   J Gen Physiol 153 (4) (2021)
   PUBMED   33651884
  REMARK    GeneRIF: Nav1.3 and FGF14 are primary determinants of the
            TTX-sensitive sodium current in mouse adrenal chromaffin cells.
REFERENCE   4  (bases 1 to 9656)
  AUTHORS   Poffers,M., Buhne,N., Herzog,C., Thorenz,A., Chen,R., Guler,F.,
            Hage,A., Leffler,A. and Echtermeyer,F.
  TITLE     Sodium Channel Nav1.3 Is Expressed by Polymorphonuclear Neutrophils
            during Mouse Heart and Kidney Ischemia In Vivo and Regulates
            Adhesion, Transmigration, and Chemotaxis of Human and Mouse
            Neutrophils In Vitro
  JOURNAL   Anesthesiology 128 (6), 1151-1166 (2018)
   PUBMED   29509584
  REMARK    GeneRIF: Nav1.3 is expressed in neutrophils in vivo and regulates
            attachment, transmigration, and chemotaxis.
REFERENCE   5  (bases 1 to 9656)
  AUTHORS   Ogiwara,I., Miyamoto,H., Tatsukawa,T., Yamagata,T., Nakayama,T.,
            Atapour,N., Miura,E., Mazaki,E., Ernst,S.J., Cao,D., Ohtani,H.,
            Itohara,S., Yanagawa,Y., Montal,M., Yuzaki,M., Inoue,Y.,
            Hensch,T.K., Noebels,J.L. and Yamakawa,K.
  TITLE     Nav1.2 haplodeficiency in excitatory neurons causes absence-like
            seizures in mice
  JOURNAL   Commun Biol 1, 96 (2018)
   PUBMED   30175250
REFERENCE   6  (bases 1 to 9656)
  AUTHORS   Spur,N.K., Bashir,R., Bushby,K., Cox,A., Cox,S., Hilde Brandt,F.,
            Hill,N., Kao,F.T., Krols,L., Marzella,R., Miller,N., Nothwang,H.G.,
            Rocchi,M., Sarfarazi,M., Stratakis,C.A., Wallgren-Petterson,C. and
            Naylor,S.
  TITLE     Report and abstracts of the Fourth International Workshop on Human
            Chromosome 2 Mapping 1996
  JOURNAL   Cytogenet Cell Genet 73 (4), 255-273 (1996)
   PUBMED   8751373
REFERENCE   7  (bases 1 to 9656)
  AUTHORS   Chin,H., Kwon,O.J., Jung,H.H., Kim,D.S. and Kozak,C.A.
  TITLE     Genetic mapping of the mouse genes encoding the voltage-sensitive
            calcium channel subunits
  JOURNAL   Genomics 28 (3), 592-595 (1995)
   PUBMED   7490102
REFERENCE   8  (bases 1 to 9656)
  AUTHORS   Malo,M.S., Srivastava,K., Andresen,J.M., Chen,X.N., Korenberg,J.R.
            and Ingram,V.M.
  TITLE     Targeted gene walking by low stringency polymerase chain reaction:
            assignment of a putative human brain sodium channel gene (SCN3A) to
            chromosome 2q24-31
  JOURNAL   Proc Natl Acad Sci U S A 91 (8), 2975-2979 (1994)
   PUBMED   8159690
REFERENCE   9  (bases 1 to 9656)
  AUTHORS   Crabbe,J.C., Belknap,J.K., Mitchell,S.R. and Crawshaw,L.I.
  TITLE     Quantitative trait loci mapping of genes that influence the
            sensitivity and tolerance to ethanol-induced hypothermia in BXD
            recombinant inbred mice
  JOURNAL   J Pharmacol Exp Ther 269 (1), 184-192 (1994)
   PUBMED   8169823
REFERENCE   10 (bases 1 to 9656)
  AUTHORS   Malo,D., Schurr,E., Dorfman,J., Canfield,V., Levenson,R. and
            Gros,P.
  TITLE     Three brain sodium channel alpha-subunit genes are clustered on the
            proximal segment of mouse chromosome 2
  JOURNAL   Genomics 10 (3), 666-672 (1991)
   PUBMED   1679748
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772235.4 and AL928621.16.
            
            On Sep 9, 2017 this sequence version replaced XM_011239392.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AL772235.4         152590-152816
            228-541             AL772235.4         183356-183669
            542-660             AL928621.16        173381-173499       c
            661-750             AL928621.16        171967-172056       c
            751-879             AL928621.16        169074-169202       c
            880-971             AL928621.16        168272-168363       c
            972-1244            AL928621.16        167181-167453       c
            1245-1308           AL928621.16        166723-166786       c
            1309-1450           AL928621.16        164369-164510       c
            1451-1657           AL928621.16        163400-163606       c
            1658-1948           AL928621.16        157049-157339       c
            1949-2245           AL928621.16        150943-151239       c
            2246-2378           AL928621.16        149724-149856       c
            2379-2617           AL928621.16        148594-148832       c
            2618-2791           AL928621.16        141444-141617       c
            2792-3148           AL928621.16        139947-140303       c
            3149-3622           AL928621.16        137500-137973       c
            3623-3743           AL928621.16        126923-127043       c
            3744-3898           AL928621.16        125620-125774       c
            3899-4072           AL928621.16        124728-124901       c
            4073-4195           AL928621.16        114856-114978       c
            4196-4468           AL928621.16        112503-112775       c
            4469-4522           AL928621.16        111589-111642       c
            4523-4660           AL928621.16        110644-110781       c
            4661-4765           AL928621.16        109499-109603       c
            4766-5036           AL928621.16        107292-107562       c
            5037-9656           AL928621.16        99695-104314        c
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 38.55 cM"
     gene            1..9656
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /note="sodium channel, voltage-gated, type III, alpha"
                     /db_xref="GeneID:20269"
                     /db_xref="MGI:MGI:98249"
     exon            1..227
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            228..541
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..6217
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sodium channel, voltage-gated, type III, alpha
                     polypeptide; voltage-gated sodium channel subtype III;
                     sodium channel protein type III subunit alpha; sodium
                     channel protein brain III subunit alpha; voltage-gated
                     sodium channel subunit alpha Nav1.3; sodium channel
                     protein type 3 subunit alpha"
                     /codon_start=1
                     /product="sodium channel protein type 3 subunit alpha
                     isoform 2"
                     /protein_id="NP_001342096.1"
                     /db_xref="GeneID:20269"
                     /db_xref="MGI:MGI:98249"
                     /translation="MAQALLVPPGPESFRLFTRESLAAIEKRAAEEKAKKPKKEQDID
                     DENKPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYVSKKTFVVLNKGKAIFRF
                     SATSALYILTPLNPVRKIAIKILVHSLFSMLIMCTILTNCVFMTLSNPPDWTKNVEYT
                     FTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTF
                     RVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNK
                     CLQWPPSDSAFEINTTSYFNGTMDSNGTFVNVTMSTFNWKDYIADDSHFYVLDGQKDP
                     LLCGNGSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLT
                     LRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLE
                     QLKKQQEEAQAVAAASAASRDFSGIGGLGELLESSSEASKLSSKSAKEWRNRRKKRRQ
                     REHLEGNHRPEGDRFPKSESEDSVKRRSFLFSLDGNPLSGDKKLCSPHQSLLSIRGSL
                     FSPRRNSKTSIFSFRGRAKDVGSENDFADDEHSTFEDSESRRDSLFVPHRPGERRNSN
                     VSQASMSSRMVPGLPANGKMHSTVDCNGVVSLGTTTETEVRKRRLSSYQISMEMLEDS
                     SGRQRAMSIASILTNTMEELEESRQKCPPCWYRFANVFLIWDCCDSWLKVKHLVNLIV
                     MDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLTVGNLVFTGIFTAEMVLKIIAMD
                     PYYYFQEGWNIFDGIIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIK
                     IIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINEDCKLPRWHMNDFFH
                     SFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVFMLVMVIGNLVVLNLFLALLLSSFSS
                     DNLAATDDDNEMNNLQIAVGRMQKGIDYVKNKIRECFRKAFFRKPKVIEIHEGNKIDS
                     CMSNNTGVVEISKELNYLKDGNGTTSGVGTGSSVEKYVIDENDYMSFINNPSLTVTVP
                     IAVGESDFENLNTEEFSSESELEESKEKLNATSSSEGSTVDVAPPREGEQAEIEPEED
                     LKPEACFTEGCIKKFPFCQVSTEEGKGKIWWNLRKTCYSIVEHNWFETFIVFMILLSS
                     GALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQTYFTNAWCWLDFL
                     IVDVSLVSLVANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIM
                     NVLLVCLIFWLIFSIMGVNLFAGKFYHCVNMTTGSMFDMSEVNNFSDCQALGKQARWK
                     NVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDVKLQPVYEENLYMYLYFVIFI
                     IFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPR
                     PANKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSKYMTLVLSRINLVFIVL
                     FTGEFLLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLA
                     RIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEA
                     GIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPDAIHPGSSVKGDCG
                     NPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKF
                     DPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTK
                     RVLGESGEMDALRIQMEDRFMASNPSKVSYEPITTTLKRKQEEVSAAIIQRNYRCYLL
                     KQRLKNISNTYDKETIKGRIVLPIKGDMVIDKLNGNSTPEKTDGSSSTTSPPSYDSVT
                     KPDKEKFEKDKPEKESKGKEV"
     exon            542..660
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            661..750
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            751..879
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            880..971
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1244
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1308
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1450
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1657
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1948
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2245
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2378
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2379..2617
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2791
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..3148
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3622
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3623..3743
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3898
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            3899..4072
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4195
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4196..4468
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4469..4522
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4523..4660
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4661..4765
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            4766..5036
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            5037..9656
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8491..8496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /note="hexamer: AATAAA"
     polyA_site      8511
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /note="major polyA site"
     regulatory      9493..9498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
                     /note="hexamer: AATAAA"
     polyA_site      9512
                     /gene="Scn3a"
                     /gene_synonym="Gm1000; Nav1.3"
ORIGIN      
        1 gtatcataga gtgaatctca gaacaggaag cggaggcata agcagaaagg attctggaaa
       61 ggtctctttg ttttcatatc cacagagaaa gcaagaaaaa aaaattgaat gtaatttgca
      121 aatccctgtg gcccaaatct gaagaactac tgggggtggc acgttccatt ctaaccatct
      181 tggatgctgt cctttgttga gctgtgattc ctaaggctct ccatcaggca attcttatgc
      241 aagaagctaa acgtaattaa atgtgcagga tgaaaagatg gcccaggcac tgctggtacc
      301 cccgggacct gagagcttcc gccttttcac tcgagaatct cttgctgcta tcgaaaagcg
      361 tgctgcagaa gagaaagcca agaaacccaa gaaagaacaa gacattgacg atgagaacaa
      421 gccaaagcca aacagtgact tggaagctgg gaagaacctt ccatttatct atggagacat
      481 tcctccagag atggtgtcgg agcctctgga ggacctggac ccctactacg tcagtaagaa
      541 aacttttgta gtgttgaata aagggaaggc aatttttcga ttcagtgcca cctccgcctt
      601 gtatatttta actccactaa accccgttag gaaaattgct attaagattt tggtacactc
      661 tttattcagc atgcttatca tgtgcactat tttgaccaac tgtgtattta tgacattgag
      721 caatcctcct gactggacga agaatgtaga gtacacattc actgggatct atacctttga
      781 gtcacttata aagatcttgg ccagaggatt ctgcttagaa gatttcacat ttcttcgtga
      841 cccatggaac tggctggatt tcagtgtcat cgtgatggca tatgtgacag agtttgtgga
      901 cctgggcaat gtctcagcgc tgagaacgtt cagagttctc cgagcattga aaacaatatc
      961 agtcattcca ggtttaaaga ccatcgtggg ggccctgatc cagtcggtga agaagctgtc
     1021 tgacgtcatg atactcactg tgttctgtct gagcgtcttt gctctcatcg ggctgcagct
     1081 cttcatgggc aacctgagga ataaatgctt gcagtggcct ccaagcgatt ctgcttttga
     1141 gatcaacact acttcctact tcaatggcac aatggactca aatgggacat ttgttaatgt
     1201 aacaatgagc acattcaact ggaaggacta tatcgcagat gacagccact tttatgtttt
     1261 ggatggacaa aaagatcctt tactttgtgg aaatgggtcc gatgcaggac aatgtccaga
     1321 agggtacatc tgtgtgaagg ctggacgaaa ccccaactac ggttacacga gctttgacac
     1381 atttagctgg gccttcttat cgctgtttcg actcatgact caagactact gggagaacct
     1441 ttaccagttg acattacgtg cagctgggaa aacctacatg atctttttcg tcctggtaat
     1501 tttcttgggc tcattttatt tggtgaactt gatcctggct gtggtggcca tggcctatga
     1561 ggaacaaaat caggccacac tggaggaggc tgagcagaaa gaggcggagt ttcagcagat
     1621 gttggagcag ttgaaaaagc agcaagagga ggctcaggcg gtggcagctg cctcagcagc
     1681 gtccagagac ttcagtggga taggagggtt aggagaactt ctggagagtt cttcagaagc
     1741 ttccaagttg agctccaaga gtgccaagga gtggaggaat cggaggaaga agaggagaca
     1801 gagggagcac ttggagggaa accacagacc cgaaggagac aggtttccca agtcggaatc
     1861 agaagacagc gtcaagcgaa ggagtttcct gttctccctg gatgggaacc cgctgagcgg
     1921 cgacaagaag ctgtgctctc cccatcagtc tctcttgagt atccgtggct ccctgttttc
     1981 cccaagacgc aatagcaaaa cgagcatttt cagcttcaga ggtcgggcga aggacgtggg
     2041 gtctgagaat gactttgcgg atgatgaaca cagcaccttt gaagatagcg agagcaggag
     2101 agactcactg tttgtgccgc acagacctgg agagcgacgc aacagtaacg ttagtcaggc
     2161 cagtatgtca tccaggatgg tgccagggct tccagccaat gggaagatgc acagcactgt
     2221 ggattgcaat ggtgtggttt ccttgggtac caccactgaa acagaagtca ggaagagaag
     2281 gctaagttct taccagatct cgatggaaat gctggaggat tcctctggga gacaaagagc
     2341 catgagcata gccagtatcc tgaccaacac gatggaggaa cttgaagaat ctagacagaa
     2401 gtgtccacca tgctggtata gatttgccaa tgtgtttttg atctgggact gttgtgattc
     2461 atggttgaaa gtaaagcatc ttgtgaattt gattgtgatg gatccatttg ttgacctggc
     2521 catcaccatc tgcatcgtgt taaacacact gttcatggcc atggagcact acccgatgac
     2581 ggagcagttc agcagtgtgc tgacggtggg aaacctggtc ttcaccggga tcttcacagc
     2641 cgagatggtc ctgaaaatca tcgcaatgga tccctattac tatttccaag agggctggaa
     2701 tatctttgat ggaattattg ttagcctgag tttaatggag cttggcctgg caaacgtgga
     2761 ggggctgtcc gtgcttcggt ccttcagact gctgcgagtc ttcaagttgg caaaatcctg
     2821 gcccacactg aatatgctca ttaagatcat cggcaactcg gtgggcgcac tgggcaacct
     2881 gaccctggtg ctggccatca tcgtcttcat ttttgccgtg gtcggcatgc agctgtttgg
     2941 aaagagctac aaggagtgtg tttgcaagat caatgaggac tgcaagctcc cgcgctggca
     3001 catgaacgac ttcttccact ccttcctgat agtgttccgc gtgctgtgtg gggagtggat
     3061 agagaccatg tgggactgca tggaggtcgc gggccagacc atgtgcctta ttgtgtttat
     3121 gttggtcatg gtgattggga accttgtggt tctgaacctc ttcctggcct tattgttgag
     3181 ttcctttagt tcagacaacc ttgctgctac ggacgatgat aacgaaatga acaacctcca
     3241 gatcgcggtg ggaaggatgc aaaaggggat tgattatgtg aaaaataaga tacgggagtg
     3301 cttccgaaaa gcgtttttta gaaagccgaa agtgatagaa atccacgaag ggaacaaaat
     3361 agacagctgc atgtccaata acacgggcgt agttgaaata agcaaagagc ttaactacct
     3421 taaagacggt aacggaacca ccagtggcgt gggtactgga agcagtgtgg agaaatacgt
     3481 aattgatgaa aatgactaca tgtcattcat caacaacccc agcctcaccg tgacggtgcc
     3541 aattgccgtg ggagagtctg actttgaaaa tttaaacacg gaagagttta gcagtgagtc
     3601 agaactggaa gaaagcaagg agaaattaaa tgcaaccagc tcttctgaag gaagcacagt
     3661 tgacgttgct ccgccccgag aaggtgaaca agctgaaatt gaacctgagg aggaccttaa
     3721 gccagaagct tgctttactg aaggatgcat taaaaaattt cccttctgcc aagtaagtac
     3781 ggaagaaggt aaaggaaaaa tctggtggaa tcttaggaag acctgctata gcattgtgga
     3841 acacaactgg tttgagacgt tcattgtgtt catgattctc ctcagtagtg gtgctttggc
     3901 ctttgaagat atatacattg agcaacggaa gaccatcaaa accatgctgg agtatgctga
     3961 caaagtcttc acttacatct tcatcctgga aatgctcctc aaatgggtgg cctatggatt
     4021 tcaaacctat ttcaccaatg cctggtgctg gttggacttc ttgattgttg atgtttcttt
     4081 ggttagcctg gtggccaacg ctcttggcta ttcagaactt ggtgccatca aatccctacg
     4141 gaccctgaga gctctgaggc cgctccgagc cttatcccgc tttgaaggca tgagggtggt
     4201 tgtgaacgct cttgttggtg caatcccctc catcatgaat gtgctactgg tgtgcctcat
     4261 cttctggtta atctttagta tcatgggtgt gaatctgttt gctggaaagt tctatcactg
     4321 tgttaacatg acaacgggca gcatgttcga catgagtgaa gtcaacaatt tcagcgactg
     4381 tcaggctctt ggcaagcaag cccgatggaa gaatgtgaaa gtcaactttg acaatgttgg
     4441 ggctggctac ctggcattgc tgcaagtggc cacattcaaa ggctggatgg atattatgta
     4501 tgcagctgtg gattcacggg acgtcaaact gcagcctgta tatgaagaaa atctgtacat
     4561 gtatctgtac tttgtcatct tcatcatctt tgggtcgttc ttcactctaa atctattcat
     4621 cggcgtcatc atagacaact tcaaccagca gaagaagaag tttggaggtc aagacatctt
     4681 tatgacagaa gagcagaaaa agtactacaa tgcaatgaag aaacttggct ccaaaaaacc
     4741 tcagaagccc atccctcgac ctgcaaacaa atttcaagga atggtctttg actttgtaac
     4801 cagacaagtg tttgacatca gcatcatgat cctcatctgc ctcaacatgg tgaccatgat
     4861 ggtggaaacg gacgaccaga gcaaatacat gaccctggtt ttgtcccgaa tcaacctggt
     4921 attcatcgtc ctcttcactg gggagtttct gctgaagctc atctctctca gatactacta
     4981 cttcacgatt ggctggaaca tctttgactt tgtggtggtg attctctcaa ttgtaggaat
     5041 gttccttgct gagctgatag agaagtattt tgtgtctcct accctgttcc gagtcatccg
     5101 cctggccagg attggacgaa tcctacgcct gatcaaaggc gccaagggga tccgcacgct
     5161 gctctttgct ctgatgatgt cccttcctgc gctgttcaac atcggcctcc tgcttttcct
     5221 cgtcatgttc atctacgcca tctttgggat gtccaacttt gcctatgtta aaaaagaggc
     5281 tggaattgat gacatgttca actttgagac ttttggcaac agcatgatct gcctgttcca
     5341 aatcaccacc tctgcgggct gggatggact gttggccccc atcctcaaca gtgcacctcc
     5401 tgactgtgac cctgatgcaa ttcaccctgg aagctcagtg aagggagact gtgggaaccc
     5461 atctgtgggg attttctttt ttgtcagcta catcatcata tccttcctgg ttgtggtgaa
     5521 catgtacatt gctgtcatcc tggagaactt cagcgttgcc acagaagaaa gtgcagagcc
     5581 cctgagtgag gacgactttg agatgttcta cgaggtctgg gagaagttcg accctgacgc
     5641 cacccagttc atagagttct gcaagctctc tgactttgca gctgccctgg atcctcccct
     5701 cctcatcgca aagccaaaca aagtccagct cattgccatg gacctgccca tggtgagtgg
     5761 agaccgcatc cactgcctgg acatcttatt tgcttttaca aagcgggtcc tgggtgagag
     5821 tggagagatg gatgcccttc gaatccagat ggaagatcgg ttcatggctt ccaatccctc
     5881 caaggtctct tatgagccca ttaccaccac tctgaagcgc aaacaagagg aggtgtctgc
     5941 tgctatcatt cagcgtaatt acagatgtta tcttttaaag caaaggttaa aaaacatatc
     6001 aaatacgtat gacaaagaga caatcaaggg gaggattgtc ttgcctataa aaggagatat
     6061 ggttattgac aaattaaatg ggaattccac cccagaaaag acagatggga gttcctctac
     6121 cacctctcct ccttcctatg acagtgtaac aaaaccagat aaggaaaagt ttgagaaaga
     6181 caaaccagaa aaagaaagca aagggaaaga ggtctgagag aatcaaaagt aaaaaaacaa
     6241 aacaaaaaaa atttcaaaaa tttaaaaagg aaacaaagaa atgtctttgt aatcaattgt
     6301 ttacagcctc tgaaggtaaa gtgtccgtgt caactggact ctaaggagag gtccatgcca
     6361 aactgactgt ttcaacaaat actcaaggtc agtgcctata ccagacagtg acctctgtca
     6421 ctgccactct gtgagacagg gtatcaacat tgacaagagg ttgctgcttc cattaccagc
     6481 tgacactgct gaggggaact ccattgtgaa agtgaccgtc atcacgccac caaacaccat
     6541 tagtacaacc aacgctcctg ttatctattt ttaacattca cattgccata tttttacaaa
     6601 atctgcccca gtgtatcttc ctggtcccca cttcatagtc tgttcataat actatgtcac
     6661 tatttttgta aatgaagtta tgttaaggga aaatatatat atatataaat cccatgttgc
     6721 taagaccaca agtttctcca gtaatcataa aaaaaatatt ttgtctgaga gatgaaatta
     6781 ttgctcaaaa ctaaggaaaa gtaaattcta atgttaccac tttgaaatac ttccactttc
     6841 tggatggtgt tagcttacaa gtgttttagt ctgttatgtt tgtatctgag cagttatgtg
     6901 cctgtaaaga ctcctctaat atttaaagga ttatttttat gcaaaatatt ttgtttcaaa
     6961 aagtgcaaat tttattctaa gtttcagagt tctatattta attttagtta aatgtcctcc
     7021 ctcaaaaaaa aattatctaa tatttctgtt ctgaaaaaaa agaaaatcta tccaaagtat
     7081 caccttagaa tacctgctcc acttactggg cagtatttct ttgccatctt ctgctctcag
     7141 caaaactgac aatttatgtc agttaaatac cctctgttat gtaaatagtt attttatcct
     7201 gtggtgcatg tttgggcaaa tatatataaa cgtatatata gcctaataca caaattctat
     7261 taaatcaaat atgtaccaca gtgtgtgtgt gtcctgagca agcttccaac agggaggtgt
     7321 cctgtatcat tcgttacaca gaaagagttt gggaggctat cactaatgca tgctaatatt
     7381 gcctacgctg ctctatttta atcaatccat ttatcacagt tttggtaaga agagtcacat
     7441 gttggtggta gaatgaattc aatctgctct ctgtctacat gtcaaaaaat tctcactgat
     7501 attaccttaa aacaccttta tttttacatt gttaattcaa cttgagtatc ataacgtgtc
     7561 tttattaatt taaaggagac acactgtata gggcctatta ccaaatccta tacttcatag
     7621 tttgctaaaa aaagaaaaaa aagtccaaaa tttgtttaaa tataaataat gtaaaatatc
     7681 atcagttttc tttgtcaaca tttgtacata agaagattat tttcgggtta atgacatggc
     7741 aatttatttt accttatcct gttgcttctt atttttaaat caaaactcca aacttttgca
     7801 tccaaaagac ttttcaatag ataatttcct aaaataaaag ttagacaatg ggttttatgg
     7861 atttctttgt tataatatat tttctaccat cctaataaga gatacgttgg tcaaaatctc
     7921 aaaccctaac cattttctac caactctggt tgcctgcaga gaatccttca ctcatggaag
     7981 tttgggtttt tttgtttatt ttttgggttt tttttttttt aactcaactt ttgtagtatt
     8041 tgcatatgcg gactaggctt atttttttaa atcctgctgc actaaaggta tcatggatct
     8101 aacacgggct ctgtcctttc taaccaggaa ccaagtggga aagttgtgca attgcctaac
     8161 atgatacaac tcttttctcc tttctcttct ttttttcaca aaccaaaagt ttagtgttca
     8221 ttccttttac aaaactcttc actgtagtat actgatgaac tgcatgcagg gaatggcgag
     8281 ctacttcgtt attgagccaa aagaataaat atttcatttt ttttttactg tatctgattt
     8341 gttggactga gtattttctt ttttaagtta tcggcaggta aaaatttaca caactaaaga
     8401 aaacctgtat ttgattcgac atttgtattc atagaagttc acatgggttt tgtaacaccc
     8461 tgctacacta tctactgaga agtttgtcaa aataaagact agttaaaagc agtttggtga
     8521 actcttcctt catcaaaggg gcagcccatt cgatcaaacc ttcgggttcg acagtactgg
     8581 cagtagccct taccactgac tgaccatttc agtttcttta ttaaattacc aagaacctaa
     8641 tctcgatgct tctgggtatc catggtgttg tgtcctaaca ttttggacac tcatgcttca
     8701 tttcatagca cactctgtag cctatgaaga attagttacc cagggattaa aaatttatgt
     8761 tagcttttca gggtttctta cagttttgtc agctcaatac agaaccttta tctcaacaga
     8821 aaactcaagt tgtaatgaat actttcacaa agacttagat ctaaatatgt atttcataat
     8881 tgccctaaaa tgttactaat gggattcatt tactggcttc tgcctagtca agtcaattta
     8941 caggtttgac tgataccaga gaagcaaaat actgattttc aaaatcctaa gtgttttaaa
     9001 gcactgtgat atataatcaa taaccaagtc tctgtaagaa accaattaat atgcattaca
     9061 ccagagccac tgtctttgaa agtaatggct tcagttgaga aagaacacct ctgaaagata
     9121 cctttctaga aaagtgttgt tatacaaaca tagaggcata gatacaattt gatagacttt
     9181 taaaataact gcttttgtac ataataaaat cttttcagct aaaatgtttt cattttagaa
     9241 tttcatgtga aaaataacaa tgacttagtc aaattcttcc tcccagaagc aacaatacta
     9301 tgacaagaaa aaaacttagt tttagtaaca agggatatca gtataaatat ctaggttttg
     9361 tttgtttgtt tgtttgtttg tttgtttctg agaaatgtga gggaaatgag ctttctgatt
     9421 ttcaattgca accaagggta ttgaactatg taaaataatg cctctgactg acatttcaag
     9481 taaataggat acaataaaca ccatgaaccc catttctatg cattgcgtcc acgtagataa
     9541 ttgttgagga agtcaatgat gacattttat aaaaatgtaa tatgatcagg gccagaaatt
     9601 ctgacccatg tcttcagctc catttttggt atcagattaa aggtgtaatt ctgtgg
//
