LOCUS       NM_001346679            8937 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus sodium channel, voltage-gated, type II, alpha (Scn2a),
            transcript variant 2, mRNA.
ACCESSION   NM_001346679 XM_006498581
VERSION     NM_001346679.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Masala,N., Mittag,M., Giovannetti,E.A., O'Neil,D.A., Distler,F.J.,
            Rupprecht,P., Helmchen,F., Yuste,R., Fuhrmann,M., Beck,H.,
            Wenzel,M. and Kelly,T.
  TITLE     Aberrant hippocampal Ca2+ microwaves following synapsin-dependent
            adeno-associated viral expression of Ca2+ indicators
  JOURNAL   Elife 13, 93804 (2024)
   PUBMED   39042440
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Wang,C., Derderian,K.D., Hamada,E., Zhou,X., Nelson,A.D.,
            Kyoung,H., Ahituv,N., Bouvier,G. and Bender,K.J.
  TITLE     Impaired cerebellar plasticity hypersensitizes sensory reflexes in
            SCN2A-associated ASD
  JOURNAL   Neuron 112 (9), 1444-1455 (2024)
   PUBMED   38412857
REFERENCE   3  (bases 1 to 8937)
  AUTHORS   Nelson,A.D., Catalfio,A.M., Gupta,J.P., Min,L.,
            Caballero-Floran,R.N., Dean,K.P., Elvira,C.C., Derderian,K.D.,
            Kyoung,H., Sahagun,A., Sanders,S.J., Bender,K.J. and Jenkins,P.M.
  TITLE     Physical and functional convergence of the autism risk genes Scn2a
            and Ank2 in neocortical pyramidal cell dendrites
  JOURNAL   Neuron 112 (7), 1133-1149 (2024)
   PUBMED   38290518
  REMARK    GeneRIF: Physical and functional convergence of the autism risk
            genes Scn2a and Ank2 in neocortical pyramidal cell dendrites.
REFERENCE   4  (bases 1 to 8937)
  AUTHORS   Arratia,L.M., Bermudes-Contreras,J.D., Juarez-Monroy,J.A.,
            Romero-Macias,E.A., Luna-Rojas,J.C., Lopez-Hidalgo,M., Vega,A.V.
            and Zamorano-Carrillo,A.
  TITLE     Experimental and computational evidence that Calpain-10 binds to
            the carboxy terminus of NaV1.2 and NaV1.6
  JOURNAL   Sci Rep 14 (1), 6761 (2024)
   PUBMED   38514708
  REMARK    GeneRIF: Experimental and computational evidence that Calpain-10
            binds to the carboxy terminus of NaV1.2 and NaV1.6.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8937)
  AUTHORS   Martin,M.S., Tang,B., Ta,N. and Escayg,A.
  TITLE     Characterization of 5' untranslated regions of the voltage-gated
            sodium channels SCN1A, SCN2A, and SCN3A and identification of
            cis-conserved noncoding sequences
  JOURNAL   Genomics 90 (2), 225-235 (2007)
   PUBMED   17544618
REFERENCE   6  (bases 1 to 8937)
  AUTHORS   Planells-Cases,R., Caprini,M., Zhang,J., Rockenstein,E.M.,
            Rivera,R.R., Murre,C., Masliah,E. and Montal,M.
  TITLE     Neuronal death and perinatal lethality in voltage-gated sodium
            channel alpha(II)-deficient mice
  JOURNAL   Biophys J 78 (6), 2878-2891 (2000)
   PUBMED   10827969
REFERENCE   7  (bases 1 to 8937)
  AUTHORS   Zha,H., Remmers,E.F., Du,Y., Goldmuntz,E.A., Mathern,P., Zhang,H.,
            Cash,J.M., Crofford,L.J. and Wilder,R.L.
  TITLE     A single linkage group comprising 11 polymorphic DNA markers on rat
            chromosome 3
  JOURNAL   Mamm Genome 5 (9), 538-541 (1994)
   PUBMED   8000136
REFERENCE   8  (bases 1 to 8937)
  AUTHORS   Sashihara,S., Yanagihara,N., Kobayashi,H., Izumi,F., Tsuji,S.,
            Murai,Y. and Mita,T.
  TITLE     Overproduction of voltage-dependent Na+ channels in the developing
            brain of genetically seizure-susceptible E1 mice
  JOURNAL   Neuroscience 48 (2), 285-291 (1992)
   PUBMED   1376448
REFERENCE   9  (bases 1 to 8937)
  AUTHORS   Malo,D., Schurr,E., Dorfman,J., Canfield,V., Levenson,R. and
            Gros,P.
  TITLE     Three brain sodium channel alpha-subunit genes are clustered on the
            proximal segment of mouse chromosome 2
  JOURNAL   Genomics 10 (3), 666-672 (1991)
   PUBMED   1679748
REFERENCE   10 (bases 1 to 8937)
  AUTHORS   Litt,M., Luty,J., Kwak,M., Allen,L., Magenis,R.E. and Mandel,G.
  TITLE     Localization of a human brain sodium channel gene (SCN2A) to
            chromosome 2
  JOURNAL   Genomics 5 (2), 204-208 (1989)
   PUBMED   2571571
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL772235.4 and BX284648.12.
            
            On Oct 26, 2016 this sequence version replaced NM_001346679.1.
            
            Summary: Voltage-gated sodium channels are transmembrane
            glycoprotein complexes composed of a large alpha subunit with four
            repeat domains, each of which is composed of six membrane-spanning
            segments, and one or more regulatory beta subunits. Voltage-gated
            sodium channels are responsible for the generation and propagation
            of action potentials in neurons and muscle. This gene encodes one
            member of the sodium channel alpha subunit gene family. In humans,
            variants of this gene are associated with seizure disorders and
            autism spectrum disorder. Mice homozygous for a knockout mutation
            die with severe hypoxia and extensive neuronal cell death, while
            gain of function mutations result in progressive seizure disorder.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: KM373687.1, KM373688.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN01164131, SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-247               AL772235.4         99067-99313         c
            248-565             AL772235.4         49320-49637         c
            566-684             AL772235.4         48362-48480         c
            685-774             AL772235.4         38995-39084         c
            775-903             AL772235.4         37957-38085         c
            904-995             AL772235.4         37301-37392         c
            996-1268            AL772235.4         36139-36411         c
            1269-1332           AL772235.4         33235-33298         c
            1333-1474           AL772235.4         31623-31764         c
            1475-1681           AL772235.4         31268-31474         c
            1682-1969           AL772235.4         29750-30037         c
            1970-2317           AL772235.4         18018-18365         c
            2318-2450           AL772235.4         12722-12854         c
            2451-2689           AL772235.4         8137-8375           c
            2690-2863           AL772235.4         6241-6414           c
            2864-3220           AL772235.4         4066-4422           c
            3221-3700           AL772235.4         2215-2694           c
            3701-3821           BX284648.12        8247-8367
            3822-3976           BX284648.12        10305-10459
            3977-4150           BX284648.12        12025-12198
            4151-4273           BX284648.12        15148-15270
            4274-4555           BX284648.12        17528-17809
            4556-4609           BX284648.12        24984-25037
            4610-4747           BX284648.12        30213-30350
            4748-4852           BX284648.12        30691-30795
            4853-5123           BX284648.12        33783-34053
            5124-8937           BX284648.12        45553-49366
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 38.61 cM"
     gene            1..8937
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /note="sodium channel, voltage-gated, type II, alpha"
                     /db_xref="GeneID:110876"
                     /db_xref="MGI:MGI:98248"
     exon            1..247
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            248..565
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..6319
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /note="sodium channel, voltage-gated, type II, alpha
                     polypeptide 1; sodium channel, voltage-gated, type II,
                     alpha 1; voltage-gated sodium channel subunit alpha
                     Nav1.2"
                     /codon_start=1
                     /product="sodium channel protein type 2 subunit alpha"
                     /protein_id="NP_001333608.1"
                     /db_xref="CCDS:CCDS84529.1"
                     /db_xref="GeneID:110876"
                     /db_xref="MGI:MGI:98248"
                     /translation="MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDE
                     DDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISR
                     FSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEY
                     TFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRT
                     FRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRN
                     KCLQWPPDNSTFEINITSFFNNSLDWNGTAFNRTMNMFNWDEYIEDKSHFYFLEGQND
                     ALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQL
                     TLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQML
                     EQLKKQQEEAQAAAAAASAESRDFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKK
                     KQKEQAGEEEKEDAVRKSASEDSIRKKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSL
                     FSPRRNSRASLFSFKGRVKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRPSN
                     VSQASRASRGIPTLPMNGKMHSAVDCNGVVSLVGGPSALTSPVGQLLPEGTTTETEIR
                     KRRSSSYHVSMDLLEDPTSRQRAMSMASILTNTMEELEESRQKCPPCWYKFANMCLIW
                     DCCKPWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLV
                     FTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGLANVEGLSVLRSFRLL
                     RVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCK
                     ISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGN
                     LVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDFVKRKIREFIQKAF
                     VRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVV
                     DESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGST
                     VDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYK
                     IVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKW
                     VAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSR
                     FEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDV
                     SVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSR
                     NVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEE
                     QKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVE
                     TDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGM
                     FLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLL
                     FLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILN
                     SGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVAT
                     EESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIA
                     MDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTT
                     LKRKQEEVSAIVIQRAYRRYLLKQKVKKVSSIYKKDKGKEDEGTPIKEDIITDKLNEN
                     STPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK"
     exon            566..684
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            685..774
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            775..903
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            904..995
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1268
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1332
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1474
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1681
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1969
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2317
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2450
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2689
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2863
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..3220
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3700
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3701..3821
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3976
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4150
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4151..4273
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..4555
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4556..4609
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4610..4747
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4748..4852
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4853..5123
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            5124..8937
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      8904
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /note="major polyA site"
     regulatory      8918..8923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /note="hexamer: AATAAA"
     polyA_site      8937
                     /gene="Scn2a"
                     /gene_synonym="6430408L10; A230052E19Rik; Nav1.2; Scn2a1"
                     /note="major polyA site"
ORIGIN      
        1 gcatgctgtt ttctgacagt gggtaccatc gagtgactga gtgtcagaac agaaagctaa
       61 ggcagaagca gagaggatgc agtgatcgcc ctgtcttggc tttttctctt tagtaaggat
      121 attattatcg cacatgtgag attttacttt ctgctgtagg ctgaattcta gaaacctgcg
      181 ttggagactg gttatattca acacgcacat ggattctgtg ctatgagtac cattcccttt
      241 atttcagcac tttcttatgc gaggagctaa acagtgatta aagaagcagg atgaaaagat
      301 ggcacaatca gtgctggtac cgccaggacc tgacagcttc cgcttcttta ccagggaatc
      361 ccttgctgct attgaacaac gcattgcaga agagaaagct aagagaccca aacaggaacg
      421 caaggacgaa gacgacgaaa atggcccaaa gccaaatagt gacttggaag caggaaaatc
      481 ccttcctttt atttatggag acattcctcc agagatggtg tcagagcccc tggaggacct
      541 ggacccctac tatatcaata agaaaacctt tatagtattg aataaaggga aagcaatctc
      601 tcggttcagt gccacctccg ccctgtacat tttaactccc ttcaacccca ttagaaaatt
      661 agctattaag attttggtac attctttatt caatgtgctt atcatgtgca caattcttac
      721 caactgtgta tttatgacca tgagcaaccc tccagactgg acaaagaatg tggagtatac
      781 ttttacagga atttatactt ttgaatcact tattaaaata cttgcaaggg gcttttgtct
      841 agaagatttc acatttctac gggatccctg gaactggctg gatttcacag tcattacctt
      901 tgcatatgtg acagagtttg tggacctggg caatgtctca gcgctgagaa cgttcagagt
      961 tctccgagca ttgaaaacaa tatcagtcat tccaggcctg aagaccatcg tgggggccct
     1021 gatccagtcg gtgaagaagc tgtctgacgt catgatactc actgtgttct gtctgagtgt
     1081 ctttgctctc atcgggctgc agctcttcat gggcaacctg aggaataaat gcttgcagtg
     1141 gcccccagac aattctacct ttgaaataaa catcacttcc ttctttaata actcattgga
     1201 ttggaatggt actgccttca ataggacaat gaacatgttt aactgggatg aatatattga
     1261 agataaaagt cacttttatt ttttggaagg acaaaacgat gctctgcttt gtggtaacag
     1321 ctcggatgct ggccagtgtc cggaaggata catctgtgtg aaggctggga gaaaccccaa
     1381 ctatggctac acgagttttg acaccttcag ctgggccttc ttgtccttat ttcgtcttat
     1441 gactcaagac ttctgggaaa acctttatca gctgaccttg cgtgctgctg ggaaaacata
     1501 tatgatattt ttcgtgcttg tgattttctt gggctcattc tacctaataa atttgatcct
     1561 ggctgtggtg gccatggcct atgaagaaca gaaccaggcc acgctggagg aagctgaaca
     1621 gaaggaggca gagtttcagc agatgctgga gcaacttaag aagcagcaag aagaagctca
     1681 ggcagcagct gcagcagcgt ctgcagaatc cagagacttc agtggagcag gtgggatagg
     1741 tgttttctcg gagagttctt ccgtagcttc aaagttaagt tccaagagtg aaaaggagct
     1801 gaaaaacaga aggaagaaaa agaaacagaa agagcaggct ggagaggaag agaaggaaga
     1861 tgctgtgcgg aaatctgcct ctgaagacag catacgaaag aaaggcttcc ggttttccct
     1921 agaagggagt agactgacct acgagaagag gttttcctct ccacaccagt ctctcttgag
     1981 catccgaggc tccctttttt ctccgagacg caacagtaga gcaagccttt tcagcttcaa
     2041 aggtcgggtg aaggatattg gttctgaaaa tgactttgcg gacgatgaac acagcacgtt
     2101 tgaggacaac gacagcagga gagactctct atttgtacca cacagacatg gagaaaggcg
     2161 tcccagcaac gttagccagg ccagccgtgc ctcccggggg atacccactc tacccatgaa
     2221 tgggaagatg cacagtgcag tggactgcaa cggtgtggtc tccctagttg gaggcccttc
     2281 tgctctcaca tctcctgtgg ggcagctact accagagggc acaactactg aaacagaaat
     2341 aaggaagagg aggtccagtt cttaccatgt ttctatggac ttgttggaag accctacatc
     2401 aaggcaaaga gcaatgagta tggccagcat tttgaccaac actatggaag aacttgaaga
     2461 atccagacag aaatgccctc catgctggta taaatttgct aatatgtgcc tgatttggga
     2521 ctgttgtaag ccatggctaa aggtaaaaca tgttgtcaat ctggtagtga tggatccatt
     2581 cgttgacctg gccatcacca tctgcattgt gttaaataca ctcttcatgg ccatggagca
     2641 ctacccgatg acggagcagt tcagcagtgt gctgtctgtt gggaatttgg tcttcactgg
     2701 gatcttcacc gcagaaatgt ttctcaagat aatagccatg gatccatatt attactttca
     2761 agaaggctgg aatatctttg atggctttat agtgagcctt agtttaatgg aactcggctt
     2821 ggcgaatgtg gaaggactgt cagttctccg atctttccga ctgcttagag tcttcaagtt
     2881 ggcaaaatcc tggcccacac tgaatatgct cattaagatc atcggcaact cggtgggcgc
     2941 gctgggcaac ctgaccctgg tgctggccat catcgtcttc atttttgccg tggtcggcat
     3001 gcagctgttt ggaaagagct acaaggagtg tgtttgcaag atttccaatg attgtgagct
     3061 gccgcgctgg cacatgcatg acttcttcca ctcgttcctg atcgtgttcc gcgtgctgtg
     3121 tggggagtgg atagagacca tgtgggactg catggaggtc gcaggccaga ccatgtgtct
     3181 tactgtcttc atgatggtca tggtgattgg gaaccttgtc gttctgaacc tcttcttggc
     3241 cttgctcctc agttctttca gctcggacaa cctggctgcc acagatgacg ataacgaaat
     3301 gaacaacctc cagatagccg tgggaaggat gcagaagggg attgattttg ttaaaaggaa
     3361 gatacgtgaa ttcattcaga aagcctttgt cagaaagcag aaagctctag atgaaatcaa
     3421 accccttgaa gatctgaata acaagaaaga cagttgtatc tccaaccaca caaccataga
     3481 aataggcaag gacctgaatt acctcaaaga tggaaacggg acgaccagtg gcataggcag
     3541 cagtgtggag aagtacgtgg tagatgagag tgattacatg tcattcatca acaaccccag
     3601 cctcaccgtg actgtgccca ttgctgtggg agagtctgac tttgaaaact taaatacaga
     3661 agaattcagc agtgaatcag atatggaaga aagcaaggag aaattgaatg caactagttc
     3721 atctgaaggc agtacagttg atataggtgc tcctgcagag ggagagcaac cagaggccga
     3781 accagaagag tcccttgagc cagaagcctg tttcacagaa gactgtgtaa gaaagttcaa
     3841 gtgttgtcag ataagcatag aagaaggcaa aggcaaactc tggtggaact tgaggaaaac
     3901 ctgctacaag atagtagagc acaactggtt tgaaaccttc atcgtcttca tgattctgct
     3961 cagcagcggt gctctggcct ttgaagacat ttacatcgag cagcgaaaaa ccatcaagac
     4021 catgctggag tatgctgaca aggtcttcac ttacatcttc atcctggaga tgttgctaaa
     4081 gtgggttgca tatggtttcc aaatgtactt caccaatgcc tggtgctggc tggacttcct
     4141 gatcgttgat gtctcactgg ttagcttaac tgcaaatgcc ttgggctatt cagaacttgg
     4201 tgccatcaaa tccctccgaa cattaagagc actgaggcct ctacgagcct tatcccgatt
     4261 tgaaggaatg agggttgttg taaacgctct cctaggtgca atcccatcca taatgaacgt
     4321 acttctggtc tgcctgatct tttggctaat attcagtatc atgggggtga atctctttgc
     4381 cggcaagttc tatcattgca tcaactacac caccggggaa atgtttgatg tgagcgtggt
     4441 caacaactac agtgagtgcc aggctctcat agagagcaat cagacagcca ggtggaagaa
     4501 tgtgaaagtc aactttgaca acgtgggact tggatatctt tctctgcttc aagtagccac
     4561 atttaaagga tggatggata tcatgtatgc agctgttgac tcaagaaatg tagaactgca
     4621 acccaaatat gaagacaatc tatacatgta cctttacttt gtcatcttta ttattttcgg
     4681 ctcattcttc acactgaacc tgttcatcgg cgtcatcata gataacttca accaacagaa
     4741 gaagaagttt ggaggtcaag acatttttat gacagaagaa cagaagaaat actacaatgc
     4801 aatgaagaag ctaggctcaa agaaaccaca aaaaccgata cctcgccctg ctaacaagtt
     4861 tcaagggatg gtctttgatt ttgtaaccaa acaagtgttt gacatcagca tcatgatcct
     4921 catctgcctc aacatggtga ccatgatggt ggaaacggac gaccagagcc aagagatgac
     4981 caacatcctg tattggatca acctggtgtt catcgtcctg ttcaccggcg agtgtgtgct
     5041 caagcttatc tcactccgtc attattattt caccattgga tggaacattt ttgattttgt
     5101 ggtggtcatc ctctccattg tagggatgtt tctcgcggag ctgatagaga agtatttcgt
     5161 gtcccctacc ctgttccgag tcatccgcct ggccaggatt ggacgaatcc tacgcctgat
     5221 caaaggcgcc aaggggatcc gcacgctgct ctttgctctg atgatgtccc ttcctgcgct
     5281 gtttaacatc ggcctcctgc ttttcctggt catgttcatc tacgccatct ttgggatgtc
     5341 caactttgcc tacgttaaga gggaagttgg gattgatgac atgttcaact ttgagacctt
     5401 tggcaacagc atgatctgcc tgttccaaat caccacttct gcgggctggg atggactgct
     5461 ggcccctata ctaaatagtg gacctcccga ttgtgaccct gaaaaagatc atcctggaag
     5521 ctcggtgaag ggagactgtg ggaacccatc tgtggggatt ttcttttttg tcagctacat
     5581 catcatatcc ttcctggttg tggtgaacat gtacattgct gtcatcctgg agaacttcag
     5641 cgttgccaca gaagaaagtg cagagcctct gagtgaggac gactttgaga tgttctacga
     5701 agtctgggag aagttcgacc ctgacgccac ccagttcata gagttctgca agctctctga
     5761 ctttgcagct gccctggatc ctcccctcct catcgcaaag ccaaacaaag tgcagctcat
     5821 tgccatggac ctgcccatgg tgagtggaga ccgcatccac tgcctggaca tcttatttgc
     5881 ttttacaaag cgggtcctgg gtgagagtgg agagatggat gctcttcgaa tccagatgga
     5941 agagcggttc atggcttcca atccctccaa ggtctcttat gagcccatta ccaccactct
     6001 gaagcgcaaa caagaggagg tgtctgctat tgtcatccag cgagcttaca gacgctatct
     6061 tctgaaacag aaagttaaga aggtttcgtc tatatataaa aaagacaagg gtaaagaaga
     6121 tgagggaacg cccatcaaag aagacatcat cacggataaa ctgaatgaga attcaactcc
     6181 agagaagact gacgtgacac cctccaccac ttcgccacct tcctacgata gcgtgaccaa
     6241 accggagaag gaaaaatttg aaaaggacaa atcagaaaaa gaggacaagg ggaaagatat
     6301 cagggaaagt aaaaagtaaa aaaataaaaa attaatgaaa attaaaaatt aaaaaaaaaa
     6361 gaatattcca ttttgtgatc aactgtttac agcctgtgtt ggtgatgtat ctgcgactcc
     6421 ctcaggaggt ctatgccaaa ctgactgttt ctataagtgt atacttaagg tcagtgccta
     6481 taaccagaca ggacctcggg tcagcaaagt gggactcgcc aaaccagaca aagagcaccg
     6541 acaggaggct tctgttttcg caaccagctg acactgctga agagtggagg agagcaatgg
     6601 ctactcagac cagaggaaca tttggaaatg gggaggaaag ttaaattttt atgtaaattt
     6661 aacatgtgac acttgatagc attaactgtc accaccgttt gtgttttaac tgccacacct
     6721 gccatatttt tacaaaatgt gtgctgtgac tttatcacct tttttttttt tttaattcac
     6781 gggttgttta ctcttatatg tgactatttt tgtaactggg tttacgtttg gggagaggga
     6841 ttaaggaggg gatactacat ttctctgtgt ataatgagat ctattttcaa tgaaggcatg
     6901 ctgcagtttt catccacaca tgacaatatc ccatcacaca cggaaagggt atacttctca
     6961 tttcaggatg tttttagatt ttttgaggtg cttaaatagc tattcatatt tttaaggtgt
     7021 ttcatccaga agaattttaa tgtgcctgta aatgttccat agagtcacaa ccatcaaaga
     7081 gttgttttat ttttacatag ttcattatat gtacatgtat atatgtatat atgtatatgg
     7141 ccgtgtatat acatatatat gtatacacac atgcatacat atgcacacac agaccatcac
     7201 atgctcagag tccaggcagc atgactataa cattttttga taagtgtcct ttggtaaaaa
     7261 aaaaaaaaaa gtatatatat atatatatcc tctcagtcct ttctaagaaa cccaaactac
     7321 caaaaaccat ccccacagcc actttctgaa gttggttctg ctttttttcc tgcagtattg
     7381 tttagccatc ttctgctctt ggtaaggttg acatagtgta tgtcaattaa aacaaaacaa
     7441 aacaaaacaa aagtctgctt tgtaaatagt aattttaccc agtggtgcat gtttgagcca
     7501 acgaaaatga tttaagcaca gtatttactg catcaaatat gtaccacagt taagtatagt
     7561 ttgcaagctt tcaacaggtg acatgacgtc attggttccc ttagagtttg aagctgtcac
     7621 tgctgcatgt ctctcttgct gctgctgctg tatcttaacc cttctgctgt tcagaagtct
     7681 aatagggaag ccatatgtca gaggtggagt gaagtggatt gttccatcag gaactcaatc
     7741 ttcacgacac tgagcaatgt gttgcagtaa acaaggaaga aatccctgct ctttgttctc
     7801 cctaccctta attgaacact ccggtgagat gtctgacagt gtaaacaagt ggcaagctac
     7861 ttagatctgt tgaaggtata tggttagagt tttctaagaa atatacatat tgtaaaaaaa
     7921 aatgggggga gttcagttta ttttatttcg aagccttttg tacataaaat gggaaattaa
     7981 aagtatcttc aggttgatgt cacagtccta tcattagttt ctgttcccgg cactttttaa
     8041 ttaaggcact ccgccaagga agagagagat aaggcctaag aggcagtaca gggctctgct
     8101 gtcattgttg gtactgtgga cttgcacata tttccacttg aaacaattct cattccaagt
     8161 ctaatgtcca tttgctttca atagtgatgc cttatcattg acaagagatt tcaaggggag
     8221 cagatgatag atactctagg cattatctga gtgggatgta ctcacctagc cttcccctcc
     8281 attcatccat cttcttaatg ttcgtttata cagatagatg gatagataga atcttactga
     8341 cccacatggc actagagctg tatcagatat gatttgggaa ccaagttttc ttctctctct
     8401 ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct ctttctctca
     8461 ctgttttcca ccaaaccagt tagtgaaatg atcttgccaa tcacaaacgt tttgtttcac
     8521 aaaaacaacc ttcattgtag atcctttata ctaaagctgt tgatttgtag tgtgttgaat
     8581 gcatgcggga aaatgctatg accatacaga gctaccggta agccacatta caatcaagcc
     8641 aaaagaataa agattttttt ttttgctttt gtttctgtat ttattgtttt gtctgtgttt
     8701 ctatcctcga gatgccagtt agaggtaaat ttctatcgtg taaaaatgat ctatctgaaa
     8761 attttaaatg taaaaagtac gcattaagta taataattca tctttaaatt tttcatggaa
     8821 tggaaactga gaagaatgta ttggatatct agttttaata ttggccaaaa tccacagata
     8881 tggtaccagg tagagtagtg gaaagttaca aaaaattaat aaaaaaattg actaaca
//
