LOCUS       NM_016717               2194 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus selenocysteine lyase (Scly), mRNA.
ACCESSION   NM_016717
VERSION     NM_016717.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Seale,L.A., Khadka,V.S., Menor,M., Xie,G., Watanabe,L.M.,
            Sasuclark,A., Guirguis,K., Ha,H.Y., Hashimoto,A.C., Peplowska,K.,
            Tiirikainen,M., Jia,W., Berry,M.J. and Deng,Y.
  TITLE     Combined Omics Reveals That Disruption of the Selenocysteine Lyase
            Gene Affects Amino Acid Pathways in Mice
  JOURNAL   Nutrients 11 (11), 2584 (2019)
   PUBMED   31717805
  REMARK    GeneRIF: Studied the role of selenocysteine lyase in amino acid
            metabolism using knockout mice and a combination of transcriptomics
            and metabolomics.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Torres,D.J., Pitts,M.W., Hashimoto,A.C. and Berry,M.J.
  TITLE     Agrp-Specific Ablation of Scly Protects against Diet-Induced
            Obesity and Leptin Resistance
  JOURNAL   Nutrients 11 (7), 1693 (2019)
   PUBMED   31340540
  REMARK    GeneRIF: Loss of Scly within a neuronal sub-population that makes
            up a small fraction of the hypothalamus produces a phenotype in
            mice distinguished by reduced weight gain while on high fat diet
            and resistance to the development of leptin insensitivity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2194)
  AUTHORS   Ogawa-Wong,A.N., Hashimoto,A.C., Ha,H., Pitts,M.W., Seale,L.A. and
            Berry,M.J.
  TITLE     Sexual Dimorphism in the Selenocysteine Lyase Knockout Mouse
  JOURNAL   Nutrients 10 (2), 159 (2018)
   PUBMED   29385050
  REMARK    GeneRIF: Selenocysteine lyase regulates energy metabolism via the
            hypothalamus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2194)
  AUTHORS   Pitts,M.W., Kremer,P.M., Hashimoto,A.C., Torres,D.J., Byrns,C.N.,
            Williams,C.S. and Berry,M.J.
  TITLE     Competition between the Brain and Testes under Selenium-Compromised
            Conditions: Insight into Sex Differences in Selenium Metabolism and
            Risk of Neurodevelopmental Disease
  JOURNAL   J Neurosci 35 (46), 15326-15338 (2015)
   PUBMED   26586820
  REMARK    GeneRIF: This study showed that Male mice lacking two key genes
            involved in Se metabolism (Scly(-/-)Sepp1(-/-) mice), selenoprotein
            P (Sepp1) and Sec lyase (Scly), develop severe neurological
            dysfunction, neurodegeneration, and audiogenic seizures.
REFERENCE   5  (bases 1 to 2194)
  AUTHORS   Seale,L.A., Gilman,C.L., Hashimoto,A.C., Ogawa-Wong,A.N. and
            Berry,M.J.
  TITLE     Diet-induced obesity in the selenocysteine lyase knockout mouse
  JOURNAL   Antioxid Redox Signal 23 (10), 761-774 (2015)
   PUBMED   26192035
REFERENCE   6  (bases 1 to 2194)
  AUTHORS   Byrns,C.N., Pitts,M.W., Gilman,C.A., Hashimoto,A.C. and Berry,M.J.
  TITLE     Mice lacking selenoprotein P and selenocysteine lyase exhibit
            severe neurological dysfunction, neurodegeneration, and audiogenic
            seizures
  JOURNAL   J Biol Chem 289 (14), 9662-9674 (2014)
   PUBMED   24519931
  REMARK    GeneRIF: These findings provide the first in vivo evidence that
            Scly and Sepp1 work cooperatively to maintain selenoprotein
            function in the mammalian brain.
REFERENCE   7  (bases 1 to 2194)
  AUTHORS   Yun,J.W., Lum,K. and Lei,X.G.
  TITLE     A novel upregulation of glutathione peroxidase 1 by knockout of
            liver-regenerating protein Reg3beta aggravates
            acetaminophen-induced hepatic protein nitration
  JOURNAL   Free Radic Biol Med 65, 291-300 (2013)
   PUBMED   23811004
  REMARK    GeneRIF: our findings unveil a new metabolic role for Reg3beta in
            protein nitration and a new biosynthesis control of GPX1 by a
            completely 'unrelated' regenerating protein, Reg3beta, via
            transcriptional activation of Scly
REFERENCE   8  (bases 1 to 2194)
  AUTHORS   Seale,L.A., Hashimoto,A.C., Kurokawa,S., Gilman,C.L., Seyedali,A.,
            Bellinger,F.P., Raman,A.V. and Berry,M.J.
  TITLE     Disruption of the selenocysteine lyase-mediated selenium recycling
            pathway leads to metabolic syndrome in mice
  JOURNAL   Mol Cell Biol 32 (20), 4141-4154 (2012)
   PUBMED   22890841
  REMARK    GeneRIF: The results suggested a dependence of glucose and lipid
            homeostasis on selenocysteine lyase activity. These findings
            connect Se and energy metabolism and demonstrate for the first time
            a unique physiological role of selenocysteine lyase.
REFERENCE   9  (bases 1 to 2194)
  AUTHORS   Raman,A.V., Pitts,M.W., Seyedali,A., Hashimoto,A.C., Seale,L.A.,
            Bellinger,F.P. and Berry,M.J.
  TITLE     Absence of selenoprotein P but not selenocysteine lyase results in
            severe neurological dysfunction
  JOURNAL   Genes Brain Behav 11 (5), 601-613 (2012)
   PUBMED   22487427
  REMARK    GeneRIF: Scly knockout mice do not display neurological dysfunction
            comparable to Sepp1 knockout mice.
REFERENCE   10 (bases 1 to 2194)
  AUTHORS   Mihara,H., Kurihara,T., Watanabe,T., Yoshimura,T. and Esaki,N.
  TITLE     cDNA cloning, purification, and characterization of mouse liver
            selenocysteine lyase. Candidate for selenium delivery protein in
            selenoprotein synthesis
  JOURNAL   J Biol Chem 275 (9), 6195-6200 (2000)
   PUBMED   10692412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102874.7 and AC110510.6.
            
            On Jan 10, 2023 this sequence version replaced NM_016717.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019879.1, AK036750.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AC102874.7         149439-149541
            104-216             AC102874.7         151621-151733
            217-317             AC102874.7         153862-153962
            318-498             AC102874.7         156367-156547
            499-626             AC102874.7         159407-159534
            627-791             AC102874.7         159744-159908
            792-898             AC102874.7         160890-160996
            899-935             AC110510.6         80522-80558
            936-1019            AC110510.6         82097-82180
            1020-1119           AC110510.6         82625-82724
            1120-1195           AC110510.6         84071-84146
            1196-2194           AC110510.6         85110-86108
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 46.08 cM"
     gene            1..2194
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /note="selenocysteine lyase"
                     /db_xref="GeneID:50880"
                     /db_xref="MGI:MGI:1355310"
     exon            1..103
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1349
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /EC_number="4.4.1.16"
                     /note="selenocysteine reductase; selenocysteine lyase;
                     Selenocysteine reductase"
                     /codon_start=1
                     /product="selenocysteine lyase"
                     /protein_id="NP_057926.2"
                     /db_xref="CCDS:CCDS15159.1"
                     /db_xref="GeneID:50880"
                     /db_xref="MGI:MGI:1355310"
                     /translation="MDAARNGALGSVESLPDRKVYMDYNATTPLEPEVIQAVTEAMKE
                     AWGNPSSSYVSGRKAKDIINAARASLAKMIGGKPQDIIFTSGGTESNNLVIHSMVRCF
                     HEQQTLKGNMVDQHSPEEGTRPHFITCTVEHDSIRLPLEHLVENQMAEVTFVPVSKVN
                     GQAEVEDILAAVRPTTCLVTIMLANNETGVIMPVSEISRRIKALNQIRAASGLPRVLV
                     HTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPRIGALYVRGVGKLTPLYPMLFGGGQ
                     EWNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNS
                     RFPGVERLPNTCNFSIQGSQLQGYTVLAQCRTLLASVGASCHSNHEDRPSPVLLSCGI
                     PVDVARNAVRLSVGRGTTRADVDLIVQDLKQAVAQLEGRL"
     exon            104..216
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            217..317
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            318..498
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            499..626
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            627..791
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            792..898
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            899..935
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1019
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1119
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1195
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..2194
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2166..2171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /note="hexamer: ATTAAA"
     polyA_site      2194
                     /gene="Scly"
                     /gene_synonym="A930015N15Rik; mSCL; SCL; Scly1; Scly2"
                     /note="major polyA site"
ORIGIN      
        1 agagacttcg gggcgctcag gcttggggtg acctgccggc gatcaggggg atggacgcgg
       61 cgcgaaatgg cgcgctgggc agtgtggaga gcctacccga caggaaggtc tatatggact
      121 ataatgccac cacacctcta gagcccgaag tcatccaggc tgtgacagag gccatgaagg
      181 aagcctgggg aaaccccagc agctcctatg tgtcaggtag gaaggccaag gacattataa
      241 atgcagctcg agcgagcctg gccaagatga taggtggaaa gccccaggac atcatcttca
      301 cttctggggg caccgagtca aataatttag taatccactc tatggtaaga tgcttccatg
      361 aacagcagac cctgaagggg aacatggttg accagcacag cccagaagag gggaccaggc
      421 cccattttat cacttgcaca gtggaacacg actccatccg gctgcccctg gagcacctgg
      481 tggaaaatca aatggcagag gtcacttttg tcccagtgtc gaaggtgaat gggcaggcag
      541 aggtcgagga tatcctggcg gctgtccgtc ccaccacatg ccttgtgacc atcatgctgg
      601 ccaataatga gaccggcgtc atcatgcctg tctccgagat cagtcggcga attaaagccc
      661 tgaaccagat ccgggctgcc tcggggctgc cccgagtcct ggtgcacaca gacgctgctc
      721 aggcactggg gaagaggcga gtggatgtcg aagacctggg cgtggacttc ctgaccatcg
      781 tgggacataa gttctacggc cccaggatcg gtgctctgta tgtacgaggg gtcggtaaac
      841 ttacccccct gtaccccatg ctgtttggag gtggacaaga gtggaatttc aggccaggga
      901 ccgagaacac gcccatgatt gctggccttg ggaaggctgc tgatctggtg agcgagaact
      961 gcgagactta tgaggcccac atgagagaca tccgagatta cctggaggag aggctggagg
     1021 ctgagtttgg taagagaatc catttgaaca gccggtttcc aggagtggag aggcttccaa
     1081 acacctgcaa cttttccatc caggggtccc agcttcaagg gtacacggtc cttgcacagt
     1141 gccggacact gctggccagt gtgggagcat catgccactc aaatcacgag gaccggccat
     1201 ccccagtgct gctgagctgc ggcattcctg tggacgtggc ccggaatgca gtccggctca
     1261 gcgtgggccg cggtaccacc agggctgatg tggatctcat cgtacaggac ctgaagcagg
     1321 ccgtggccca actggaaggg cggctctaga actagcagcc gctctctgta gccagctgga
     1381 cttttaccac tcgatccata ggaaaccgct tgcaaaagcc cttggtccct aagctgcccc
     1441 atctcaagag ctgcgttgag gtgacttgtc ctactgtggg gtgcagagtg acgaggggct
     1501 gcccaccgca cagaatccca ccccagcacc catccttaca agctcaggac agccatgtca
     1561 cataggactt ctccctgtca ccttctgaca aaggccacag gggaatagga atattccagt
     1621 tatcgggcag cctgccagga ccgcctgacc ccagaaggca ccttggtcag aagacaaaac
     1681 ccaagtcttg gtccggtgtc acttagctgc tagtgtgaga ataggaaagt tgattttgac
     1741 tccccctcct acccccaacc ccccaccccc gtgcttcccc tgtgcaccta cagacacacc
     1801 cttctccagt gcagccctgc ctctgccctc agccagccac tgcccacctg cctccctggc
     1861 ctgatggtcc gcagatgtcc catgccttgt ccccggaagt cgaagcttac cctgccttct
     1921 actcagggat ccacctccag cgtggtaccg ggaggctggt gcctgagctc agtctttgcc
     1981 aaagtgggaa atctgatcat ttctcgctca gattccattt taattttatt ttgcaaaagg
     2041 ctgtgccatc ttaaggatgt gtggcacaca tcgcaccaag gtttatatag gaatgtcttc
     2101 ctggaggctg ctttttgggt ctgtggacca aaggatgacg caacccgtga aaacagttgt
     2161 cagaaattaa atgaactaat gaaacatgtc tgca
//
