LOCUS       NM_029825               2176 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Sec1 family domain containing 1 (Scfd1), transcript
            variant 1, mRNA.
ACCESSION   NM_029825 XM_126906
VERSION     NM_029825.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Slater,L.H., Hett,E.C., Clatworthy,A.E., Mark,K.G. and Hung,D.T.
  TITLE     CCT chaperonin complex is required for efficient delivery of
            anthrax toxin into the cytosol of host cells
  JOURNAL   Proc Natl Acad Sci U S A 110 (24), 9932-9937 (2013)
   PUBMED   23716698
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161116.4, BY210008.1, AK044943.1 and CO044480.1.
            
            On Apr 16, 2015 this sequence version replaced NM_029825.2.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK044943.1, AK014070.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC161116.4         89586-89588
            4-67                BY210008.1         1-64
            68-2158             AK044943.1         3-2093
            2159-2176           CO044480.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 22.11 cM"
     gene            1..2176
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /note="Sec1 family domain containing 1"
                     /db_xref="GeneID:76983"
                     /db_xref="MGI:MGI:1924233"
     exon            1..124
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..1992
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /note="isoform 1 is encoded by transcript variant 1; sec1
                     family domain-containing protein 1; syntaxin-binding
                     protein 1-like 2"
                     /codon_start=1
                     /product="sec1 family domain-containing protein 1 isoform
                     1"
                     /protein_id="NP_084101.1"
                     /db_xref="CCDS:CCDS36439.1"
                     /db_xref="GeneID:76983"
                     /db_xref="MGI:MGI:1924233"
                     /translation="MVGSKMAATASIRERQTVALKRMLNFNVPHVKNSTGEPVWKVLI
                     YDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAVYFVMPTEENIDRLCQD
                     LRNQLYESYYLNFISAISRSKLEDIANAALAASAVTQVAKVFDQYLNFITLEDDMFVL
                     CNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMV
                     AVKLDKKLRENLRDARNSLFTGDPLGTGQFSFQRPLLVLVDRNIDLATPLHHTWTYQA
                     LVHDVLDFHLNRVNLEESTGVENSPAGARPKRKNKKSYDLTPVDKFWQKHKGSPFPEV
                     AESVQQELESYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEK
                     KRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDVISDPDAGTPEDK
                     MRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNLSPLQYIKQWKAFAKMASTPASYGN
                     TTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYF
                     DPKMLRSNDSSVPRNKSPFQEAIVFVVGGGNYIEYQNLVDYIKAKQGKHILYGCSEIF
                     NATQFIKQLSQLGQK"
     exon            125..195
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            196..284
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            285..375
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            376..498
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            499..586
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            587..676
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            677..732
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            733..818
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            819..918
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1058
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1149
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1223
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1305
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1402
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1473
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1553
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1616
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1692
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1746
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1799
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1833
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1899
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1968
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2164
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2144..2149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /note="hexamer: AATAAA"
     polyA_site      2164
                     /gene="Scfd1"
                     /gene_synonym="3110021P21Rik; RA410; STXBP1L2"
                     /note="major polyA site"
ORIGIN      
        1 tcctggttct gcttccggcg cgggaggtag ggccacgtca tcttcgggct cccagtgcca
       61 gccgggcagt ggatggtggg aagcaagatg gcggccacgg ccagcattcg ggagaggcaa
      121 acagtggctc tgaagcggat gttgaatttc aatgtgcctc atgttaaaaa cagtactgga
      181 gaaccagtat ggaaggtact catttatgac agatttggcc aagatatcat ctctcctctg
      241 ctgtctgtga aggaactgag agacatgggc atcactctgc atctcctttt gcactcagac
      301 cgagatccga ttccagatgt tcctgcagtg tactttgtga tgccaaccga agaaaatatt
      361 gacagactgt gccaggatct tcgaaatcag ctctatgaat cctattattt aaattttatt
      421 tctgcgattt caagaagtaa actggaagac attgcgaatg cagcattggc cgctagtgca
      481 gtcacacaag ttgccaaggt ttttgaccag tatctcaatt ttattacttt ggaagatgac
      541 atgtttgtat tatgtaatca aaataaggaa cttgtttcat atcgtgccat taacaggcca
      601 gacatcacag acacagagat ggaaactgtt atggacacta ttgtggacag cctcttctgc
      661 ttttttgtta cattaggtgc tgttcccatc atccgatgct caagaggaac ggcggcagaa
      721 atggtggcag tgaaactaga taaaaaactt cgggaaaatc taagagatgc aagaaacagc
      781 cttttcaccg gtgatccact tggaactggc cagttcagct tccaaaggcc tttattagtc
      841 cttgttgaca gaaacattga cctggcaacg cctttacacc atacgtggac ataccaagcg
      901 ctggtacacg atgtgctgga tttccactta aacagagtaa atttggaaga atctacagga
      961 gtggaaaact ctccagctgg tgctagacca aagaggaaaa acaagaagtc ttacgactta
     1021 actccagttg ataaattctg gcagaagcat aaaggaagtc cattcccaga agtcgcagaa
     1081 tcagtccaac aagaactaga atcttacaga gcacaagaag atgaggtcaa acgactgaag
     1141 agcattatgg gactagaagg agaggatgaa ggggccatca gcatgctttc tgataacact
     1201 gctaagctca catcagctgt cagttctttg ccagaactcc ttgaaaaaaa aagacttatt
     1261 gatctgcaca caaatgttgc cactgctgtt ttagaacaca taaaggcaag aaaactggat
     1321 gtatattttg aatatgaaga aaaaataatg agcaagacta ctctggataa gtccctccta
     1381 gacgtcatat ccgaccctga tgcagggact ccggaagaca aaatgaggct gtttcttatc
     1441 tattacataa gcgcacagca agcaccgtcc gaggttgatt tggagcagta taaaaaggct
     1501 ttaacagatg caggctgcaa ccttagccct ttacagtata tcaagcagtg gaaggctttt
     1561 gccaagatgg cctcaactcc tgccagctac ggaaacacta ccactaaacc aatgggtctc
     1621 ctgtcccgag tcatgaatac aggatcacag tttgtgatgg aaggtgtgaa gaacctggta
     1681 ttgaagcagc agaatctacc tgttactcgg attttagaca atctcatgga gatgaagtca
     1741 aaccctgaaa ctgatgatta tagatatttt gatcccaaaa tgctgcggag taatgacagc
     1801 tcagttccta ggaacaaaag tccattccaa gaggccattg tctttgtggt aggaggaggc
     1861 aactatattg aatatcagaa tcttgttgac tacataaagg caaagcaagg caagcatatc
     1921 ttatatggct gcagtgagat ttttaatgct acacagttca taaaacagct gtcacagctt
     1981 ggacaaaaat aacacagaag aaccacaatg gggtaatcag tttggataga tgtaaaaaga
     2041 gccagatatg tccttctcca tggccatgcc ctaactgcaa tctgtcagaa tcagtcattt
     2101 ttaaagaaat tctatacttc atatactgta taatgattaa aataataaac catttcagaa
     2161 ataaaaaaaa aaaaaa
//
