LOCUS       NM_001001144            4226 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus SREBF chaperone (Scap), transcript variant 1, mRNA.
ACCESSION   NM_001001144 XM_135190
VERSION     NM_001001144.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Li,X., Tang,X., Xiang,Y., Zhao,Z., Li,Y., Ding,Q., Zhang,L., Xu,J.,
            Zhao,L. and Chen,Y.
  TITLE     N-glycosylation of SCAP exacerbates hepatocellular inflammation and
            lipid accumulation via ACSS2-mediated histone H3K27 acetylation
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 326 (6), G697-G711 (2024)
   PUBMED   38591127
  REMARK    GeneRIF: N-glycosylation of SCAP exacerbates hepatocellular
            inflammation and lipid accumulation via ACSS2-mediated histone
            H3K27 acetylation.
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Huang,Y., Wang,Y.F., Ruan,X.Z., Lau,C.W., Wang,L. and Huang,Y.
  TITLE     The role of KLF2 in regulating hepatic lipogenesis and blood
            cholesterol homeostasis via the SCAP/SREBP pathway
  JOURNAL   J Lipid Res 65 (1), 100472 (2024)
   PUBMED   37949368
  REMARK    GeneRIF: The role of KLF2 in regulating hepatic lipogenesis and
            blood cholesterol homeostasis via the SCAP/SREBP pathway.
REFERENCE   3  (bases 1 to 4226)
  AUTHORS   Lundgren,P., Sharma,P.V., Dohnalova,L., Coleman,K., Uhr,G.T.,
            Kircher,S., Litichevskiy,L., Bahnsen,K., Descamps,H.C.,
            Demetriadou,C., Chan,J., Chellappa,K., Cox,T.O., Heyman,Y.,
            Pather,S.R., Shoffler,C., Petucci,C., Shalem,O., Raj,A., Baur,J.A.,
            Snyder,N.W., Wellen,K.E., Levy,M., Seale,P., Li,M. and Thaiss,C.A.
  TITLE     A subpopulation of lipogenic brown adipocytes drives thermogenic
            memory
  JOURNAL   Nat Metab 5 (10), 1691-1705 (2023)
   PUBMED   37783943
REFERENCE   4  (bases 1 to 4226)
  AUTHORS   Yan,C., Zheng,L., Jiang,S., Yang,H., Guo,J., Jiang,L.Y., Li,T.,
            Zhang,H., Bai,Y., Lou,Y., Zhang,Q., Liang,T., Schamel,W., Wang,H.,
            Yang,W., Wang,G., Zhu,Z.J., Song,B.L. and Xu,C.
  TITLE     Exhaustion-associated cholesterol deficiency dampens the cytotoxic
            arm of antitumor immunity
  JOURNAL   Cancer Cell 41 (7), 1276-1293 (2023)
   PUBMED   37244259
REFERENCE   5  (bases 1 to 4226)
  AUTHORS   Fei,X., Huang,J., Li,F., Wang,Y., Shao,Z., Dong,L., Wu,Y., Li,B.,
            Zhang,X., Lv,B., Zhao,Y., Weng,Q., Chen,K., Zhang,M., Yang,S.,
            Zhang,C., Zhang,M., Li,W., Ying,S., Sun,Q., Chen,Z. and Shen,H.
  TITLE     The Scap-SREBP1-S1P/S2P lipogenesis signal orchestrates the
            homeostasis and spatiotemporal activation of NF-kappaB
  JOURNAL   Cell Rep 42 (6), 112586 (2023)
   PUBMED   37267109
REFERENCE   6  (bases 1 to 4226)
  AUTHORS   Horton,J.D., Shah,N.A., Warrington,J.A., Anderson,N.N., Park,S.W.,
            Brown,M.S. and Goldstein,J.L.
  TITLE     Combined analysis of oligonucleotide microarray data from
            transgenic and knockout mice identifies direct SREBP target genes
  JOURNAL   Proc Natl Acad Sci U S A 100 (21), 12027-12032 (2003)
   PUBMED   14512514
  REMARK    GeneRIF: 1,003 genes showed statistically significant increased
            expression in livers of transgenic SREBP-1a mice, 505 increased in
            livers of transgenic SREBP-2 mice, and 343 showed decreased
            expression in Scap-/- livers.
REFERENCE   7  (bases 1 to 4226)
  AUTHORS   Yabe,D., Brown,M.S. and Goldstein,J.L.
  TITLE     Insig-2, a second endoplasmic reticulum protein that binds SCAP and
            blocks export of sterol regulatory element-binding proteins
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 12753-12758 (2002)
   PUBMED   12242332
REFERENCE   8  (bases 1 to 4226)
  AUTHORS   Wittenburg,H., Lammert,F., Wang,D.Q., Churchill,G.A., Li,R.,
            Bouchard,G., Carey,M.C. and Paigen,B.
  TITLE     Interacting QTLs for cholesterol gallstones and gallbladder mucin
            in AKR and SWR strains of mice
  JOURNAL   Physiol Genomics 8 (1), 67-77 (2002)
   PUBMED   11842132
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4226)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 4226)
  AUTHORS   Matsuda,M., Korn,B.S., Hammer,R.E., Moon,Y.A., Komuro,R.,
            Horton,J.D., Goldstein,J.L., Brown,M.S. and Shimomura,I.
  TITLE     SREBP cleavage-activating protein (SCAP) is required for increased
            lipid synthesis in liver induced by cholesterol deprivation and
            insulin elevation
  JOURNAL   Genes Dev 15 (10), 1206-1216 (2001)
   PUBMED   11358865
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC070437.1, BC072633.1 and BM250223.2.
            
            On Nov 8, 2013 this sequence version replaced NM_001001144.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070437.1, AK146658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2102              BC070437.1         1-2102
            2103-4223           BC072633.1         1976-4096
            4224-4226           BM250223.2         1-3                 c
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.91 cM"
     gene            1..4226
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /note="SREBF chaperone"
                     /db_xref="GeneID:235623"
                     /db_xref="MGI:MGI:2135958"
     exon            1..150
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            151..369
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..4078
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /note="Sreb cleavage-activating protein; SREBP cleavage
                     activating protein"
                     /codon_start=1
                     /product="sterol regulatory element-binding protein
                     cleavage-activating protein"
                     /protein_id="NP_001001144.2"
                     /db_xref="CCDS:CCDS23564.1"
                     /db_xref="GeneID:235623"
                     /db_xref="MGI:MGI:2135958"
                     /translation="MTLTERLREKISQAFYNHGLLCASYPIPIILFTGLCILACCYPL
                     LKLPLPGTGPVEFSTPVKGYSPPPADSDHKQGEPSEQPEWYVGAPVAYIQQIFVKSSV
                     SPWHRNLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGTKSLEEVCLQVTDLLPGLRKL
                     RSLLPEHGCLLLSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTSATLKDLLFGVPG
                     KYSGVSLYTRKRMVSYTITLVFQRYHAKFLSSLRARLMLLHPSPNCSLRAENLVHVHF
                     KEEIGIAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLC
                     TLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSI
                     MKNAATELGIILIGYFTLVPAIQEFCLFAVVGLVSDFFLQMLFFTTVLSIDIRRMELA
                     DLNKRLPPESCLPSAKPVGRPARYERQQAVRPSTPHTITLQPSSFRNLRLPKRLRVIY
                     FLARTRLAQRLIMAGTVVWIGILVYTDPAGLRTYLAAQVTEQSPLGEGSLGPMPVPSG
                     VLPASHPDPAFSIFPPDAPKLPENQTLPGELPEHAGPAEGVHDSRAPEVTWGPEDEEL
                     WRKLSFRHWPTLFNYYNITLAKRYISLLPVIPVTLHLNPREALEGRHPQDGRSAWAPQ
                     EPLPAGLWESGPKGPGGTQTHGDITLYKVAALGLAAGIVLVLLLLCLYRVLCPRNYGQ
                     PGGGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLASDGMLLVSCCLAGQV
                     CVWDAQTGDCLTRIPRPGPRRDSCGGGAFETQENWERLSDGGKASPEEPGDSPPLRRR
                     PRGPPPPSLFGDQPDLTCLIDTNFSVQLPPEPTQPEPRHRVGCGRSRDSGYDFSRLVQ
                     RVYQEEGLAAMRMPALRPPSPGPPLPQASQEEGTAPEKGSPPLAWTPSTAGSIWSLEL
                     QGNLIVVGRSSGRLEVWDAIEGVLCCSNEEISSGITALVFLDRRIVAARLNGSLDFFS
                     LETHTSLSPLQFRGTPGRGSSPSSSVYSSSNTVTCHRTHTVPCAHQKPITALRAAAGR
                     LVTGSQDHTLRVFRLDDSCCLFTLKGHSGAITAVYIDQTMVLASGGQDGAICLWDVLT
                     GSRVSQTFAHRGDVTSLTCTASCVISSGLDDFISIWDRSTGIKLYSIQQDLGCGASLG
                     VISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVCNFGSE
                     LSLVYVPSVLEKLD"
     exon            370..499
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            500..657
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            658..878
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            879..984
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1157
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1284
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1397
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1492
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1591
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1810
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..2187
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2376
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2578
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            2579..2694
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..3208
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3209..3294
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..3377
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3378..3544
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3545..3625
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3626..3832
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     exon            3833..4226
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4200..4205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /note="hexamer: AATAAA"
     polyA_site      4223
                     /gene="Scap"
                     /gene_synonym="9530044G19; mKIAA0199"
                     /note="major polyA site"
ORIGIN      
        1 gttgagaggt gaaggggcgg ggagctgcgc gggcgccggg cggccgggag ggagagggcg
       61 ggctccaaac accggaccgc gggccaggag cgcgcaggcc gttctccgcc gctcggtcgc
      121 cgccgcccgg gagctgcctc gctgccacag gtgcctgcag atgatgtctg ctgtaagtga
      181 tatccagcat cttccgggct gatccatggt cactttccgg gatggcaaca aggtgactta
      241 gccgaggatg accctgactg aaaggcttcg tgagaagata tctcaggcct tctacaacca
      301 tgggctgctc tgcgcatcct atccaattcc catcatcctc ttcacaggac tctgcatctt
      361 agcctgctgc tacccgctgc tgaagctccc cttgcctgga acgggacctg tggaattctc
      421 cacgcctgtg aagggttact cgcccccgcc tgcggactct gaccacaaac aaggagagcc
      481 cagcgagcag ccagagtggt atgtgggtgc ccccgtggcg tacatccaac agatatttgt
      541 gaagtcatcg gtgtctccct ggcacagaaa tcttctggca gtcgatgtgt tccggtcacc
      601 tctgtcccga gcattccaac tggtggaaga gatccggaac catgtgctga gagacagctc
      661 agggaccaag agcctggagg aggtttgcct gcaggtgaca gacctgctgc caggcctcag
      721 gaaactccgg agcctacttc ccgaacatgg ctgcctgctg ctgtcccctg ggaacttctg
      781 gcagaatgat tgggagagat tccatgccga ccctgacatc attgggacca tccatcaaca
      841 tgagcccaaa actctacaga catcagccac actcaaagac ttgctgtttg gtgttcctgg
      901 gaagtacagt ggggtgagcc tctacacaag gaaaaggatg gtctcctaca ccatcaccct
      961 ggtcttccag cgctaccatg ccaagtttct gagcagccta cgtgcccggc tcatgctgct
     1021 gcaccccagc cccaactgca gcctccgagc agagaacctg gtccacgtcc acttcaaaga
     1081 ggagattggc attgctgagc tcatcccgct cgtgaccacc tacatcatcc tgtttgccta
     1141 catctacttc tccacacgca agatcgacat ggtcaagtcc aagtggggcc tcgccctggc
     1201 agccgtggtc acagtactta gctcactgct catgtctgtg gggctctgca ccctcttcgg
     1261 cctgacgccc acactcaatg gcggtgagat cttcccatac ctggtggtcg ttattgggct
     1321 agagaacgtg ttggtgctca ccaagtcagt ggtatcaact ccagtggacc tcgaggtgaa
     1381 gcttcggatt gcacaaggct tgagtagtga gagctggtcc atcatgaaga acgcggcgac
     1441 cgagctgggc atcatcctca ttggctactt caccctcgtg cctgctatcc aggagttctg
     1501 cctctttgct gttgtgggcc tggtgtctga cttcttcctc cagatgctgt tcttcaccac
     1561 tgtcctgtcg atcgacattc gccggatgga gctagcagac ctaaacaagc ggctgccccc
     1621 tgaatcctgc ctgccctcag ccaagcccgt ggggaggcca gcacgatatg agagacagca
     1681 ggctgtacgg ccatccacgc cacacaccat cacattgcaa ccatcttcct tccgaaacct
     1741 gcggcttccc aaaaggctgc gtgtcatcta cttcctggcc cgcactcgcc tggcccagcg
     1801 cctcatcatg gctggtaccg ttgtctggat tggcatcctg gtatacaccg acccggcagg
     1861 gctgcgcacc taccttgctg cccaggtgac agagcagagc ccactgggtg agggttccct
     1921 gggccccatg cctgtgccta gcggagtgct gcctgccagc cacccggacc ctgcattctc
     1981 catcttccca cctgatgctc ctaaactgcc agagaaccag accttgccag gtgagctgcc
     2041 tgagcatgct ggtccagcag agggtgtcca tgacagccga gccccagagg taacttgggg
     2101 gcctgaggat gaggagctgt ggaggaaatt gtccttccgc cactggccca cactcttcaa
     2161 ctactacaac atcacactgg ccaaaaggta catcagcctg ctgcctgtca tccctgtcac
     2221 actacacctg aatccacggg aggctctgga ggggcgacac cctcaggatg gtcgcagtgc
     2281 ctgggcccca caagagcctt tgcccgctgg cctctgggag tccggaccta agggaccagg
     2341 tggaacacag acccatggcg acattacctt gtacaaggtg gccgcgcttg gcctagcagc
     2401 gggcatcgtc ctggtgctgc tgctgctctg cctctaccgg gtgctctgcc cgcgtaatta
     2461 tgggcagccg ggtggtggcc ccggcaggcg gaggcgcggg gagctgccct gcgatgacta
     2521 cggctacgca ccgcccgaga cggagatagt gccgctggtg ctgcgaggtc acctcatgga
     2581 catcgagtgt ctggctagcg atgggatgct actagtgagc tgctgcctgg caggccaagt
     2641 ctgcgtgtgg gacgctcaga caggggactg cctcacacgg atcccacgcc cagggccacg
     2701 ccgggatagc tgcggaggtg gagcttttga gactcaggag aactgggaaa ggctgtcaga
     2761 tggaggcaag gctagcccgg aagaacctgg agacagccct ccgctgcgac gacgcccccg
     2821 agggcctcca ccgccttccc tctttgggga ccagcccgac ctcacctgct taatcgacac
     2881 caacttctcg gtgcagctgc ccccagagcc cactcagccc gagcctcggc accgggtggg
     2941 ctgtggccgc tctagagact cgggttatga cttcagccgc ctggtgcagc gtgtgtacca
     3001 ggaggaaggc ctggctgcta tgcgcatgcc ggccctgcgc ccaccctccc ctggacctcc
     3061 cttgccccag gcctctcaag aagaggggac tgcacctgag aagggctccc ctcccctggc
     3121 ctggaccccc agcacagccg gttccatctg gagcttagag ctgcaaggca atctcatcgt
     3181 ggttgggcgg agcagcggcc ggctggaggt gtgggacgcc attgaggggg tgctctgctg
     3241 cagcaatgag gagatctcct caggcatcac agcccttgtc ttcttggaca ggaggattgt
     3301 agctgctcgg cttaatggtt cccttgattt cttttctttg gagacccaca cttccctcag
     3361 ccccctgcag ttcagaggga ccccagggcg aggcagttct ccttcctcat ctgtgtacag
     3421 cagcagcaac acagtgacct gtcatcggac ccacacagtg ccctgtgcac accagaagcc
     3481 catcacagcc ctgagagctg ctgccgggcg cctagtgaca gggagccaag accatactct
     3541 aagagtcttc cgactggatg actcgtgttg cctctttacc ctgaagggcc actcaggggc
     3601 aatcacagct gtgtacattg atcagaccat ggtactggcc agtggaggac aagatggagc
     3661 catctgcctg tgggatgtac taacaggcag ccgggtcagc caaacatttg ctcaccgtgg
     3721 agatgttacc tccctcacct gtaccgcttc ctgtgtcatt agtagtggcc tggatgactt
     3781 catcagtatc tgggaccgca gcacaggcat caagctgtac tccattcagc aggacctggg
     3841 ctgtggtgca agcttgggtg tcatctcaga taaccttctg gtgaccggcg gccagggctg
     3901 tgtctccttt tgggacctaa actatgggga cctgttacag acagtctact tgggcaagaa
     3961 cagtgaagcc cagcctgccc ggcagatttt ggtgttggac aatgctgcca ttgtctgcaa
     4021 ctttggcagt gagctcagcc tagtgtatgt gccctctgtg ctggagaaac tggactgaag
     4081 gcaggtcaag tacgctattc cctttccccc atcccaaggt ggggcacagg ggatagcaac
     4141 tctttggacc tagactagag gcaatagctg actctgaact gttgtctcct gactgtaata
     4201 ataaactttt ttaaaaaacc acattt
//
