LOCUS       NM_022813               2370 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus secretory carrier membrane protein 2 (Scamp2),
            transcript variant 2, mRNA.
ACCESSION   NM_022813
VERSION     NM_022813.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Zaarour,N., Defontaine,N., Demaretz,S., Azroyan,A., Cheval,L. and
            Laghmani,K.
  TITLE     Secretory carrier membrane protein 2 regulates exocytic insertion
            of NKCC2 into the cell membrane
  JOURNAL   J Biol Chem 286 (11), 9489-9502 (2011)
   PUBMED   21205824
  REMARK    GeneRIF: SCAMP2 regulates NKCC2 transit through recycling endosomes
            and limits the cell surface targeting of the co-transporter by
            interfering with its exocytotic trafficking.
REFERENCE   3  (bases 1 to 2370)
  AUTHORS   Toonen,R.F., de Vries,K.J., Zalm,R., Sudhof,T.C. and Verhage,M.
  TITLE     Munc18-1 stabilizes syntaxin 1, but is not essential for syntaxin 1
            targeting and SNARE complex formation
  JOURNAL   J Neurochem 93 (6), 1393-1400 (2005)
   PUBMED   15935055
REFERENCE   4  (bases 1 to 2370)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   5  (bases 1 to 2370)
  AUTHORS   Fernandez-Chacon,R. and Sudhof,T.C.
  TITLE     Novel SCAMPs lacking NPF repeats: ubiquitous and synaptic
            vesicle-specific forms implicate SCAMPs in multiple
            membrane-trafficking functions
  JOURNAL   J Neurosci 20 (21), 7941-7950 (2000)
   PUBMED   11050114
REFERENCE   6  (bases 1 to 2370)
  AUTHORS   Singleton,D.R., Wu,T.T. and Castle,J.D.
  TITLE     Three mammalian SCAMPs (secretory carrier membrane proteins) are
            highly related products of distinct genes having similar
            subcellular distributions
  JOURNAL   J Cell Sci 110 (Pt 17), 2099-2107 (1997)
   PUBMED   9378760
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK161002.1, AF295402.1, CA979044.1 and CX217627.1.
            
            On Nov 15, 2007 this sequence version replaced NM_022813.2.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK161002.1, AK171766.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               AK161002.1         1-428
            429-1256            AF295402.1         433-1260
            1257-1954           CA979044.1         66-763
            1955-2370           CX217627.1         41-456
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 31.05 cM"
     gene            1..2370
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /note="secretory carrier membrane protein 2"
                     /db_xref="GeneID:24044"
                     /db_xref="MGI:MGI:1346518"
     exon            1..167
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1100
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     secretory carrier-associated membrane protein 2"
                     /codon_start=1
                     /product="secretory carrier-associated membrane protein 2
                     isoform 2"
                     /protein_id="NP_073724.1"
                     /db_xref="CCDS:CCDS23224.1"
                     /db_xref="GeneID:24044"
                     /db_xref="MGI:MGI:1346518"
                     /translation="MSAFDTNPFADPVDVNPFQDPSVTQLTNAPQSGLAEFNPFSETN
                     AATTVPATQAPGPSQPAVLQPSVEPAQPTPQAVAAAAQAGLLRQQEELDRKAAELERK
                     ERELQNTAANLHVRDNNWPPLPSWCPVKPCFYQDFSTEIPADYQRICKMLYYLWMLHS
                     VTLFLNLLACLAWFTSDAANGTAFGLSILWFLIFTPCAFLCWYRPIYKAFRSDNSFSF
                     FVFFFVFFCQIGIYFIQLIGLPNLGTSGWLAALSTMKNGPLAVTIIMMVVAGFFTLCA
                     GLSLFLLQRVHAFYRRTGASFQQAQEEFSQGIFSSRTFRGAASSAARGAFQGN"
     exon            168..236
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            237..335
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..453
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            454..582
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            583..742
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            743..844
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            845..965
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     exon            966..2342
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2320..2325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /note="hexamer: AATAAA"
     polyA_site      2342
                     /gene="Scamp2"
                     /gene_synonym="Sc2"
                     /note="major polyA site"
ORIGIN      
        1 agaacggctg ccttgctccc ggaagtggag ggtttacact gtgtgccgct gggtctgagt
       61 gaccaaaggc agtagcgctc gcggagatca cccgctggcc ctcgatcacc atgtcggcct
      121 tcgacactaa ccccttcgcg gacccagtgg acgtaaaccc cttccaggat ccctctgtga
      181 cccagctgac caatgctcct cagagtggcc tggctgagtt caatcccttc tcagagacaa
      241 atgcagcgac aacagttcct gccacacaag ctcctgggcc ctcccagcca gcagttctcc
      301 agccctcagt ggaaccagca cagccaacgc cccaggctgt tgcagctgcg gcccaggcag
      361 gcttgcttcg acagcaggaa gaactagaca ggaaagctgc cgagctggaa cgcaaggagc
      421 gagagttgca gaacactgca gcgaatttgc atgtgcgaga caacaactgg ccgccactcc
      481 cctcatggtg ccctgtgaaa ccctgcttct atcaggactt ctccacggag atccctgctg
      541 actaccagcg gatttgcaag atgctttact atctctggat gttgcattca gtgactctgt
      601 ttctaaacct gcttgcgtgc ctggcctggt tcacaagtga cgcagccaat ggaacagcct
      661 ttggcctctc catcctttgg tttctgatct tcactccctg tgccttcctt tgttggtacc
      721 gacccatcta caaggctttc aggtctgaca actctttcag cttcttcgtg ttcttttttg
      781 tatttttttg tcaaataggg atctacttca tccagttgat tggcttgcct aacttgggga
      841 ccagtggttg gcttgcagcc ctatctacga tgaaaaatgg gcccttagct gtgaccatca
      901 tcatgatggt agtggctggc ttctttaccc tctgtgctgg gctttcactc ttcctcctgc
      961 agcgggtaca cgccttctac cgccgaacag gagccagctt ccagcaggcc caggaagagt
     1021 tctcccaggg tatcttcagc agcagaacct ttcgcggggc tgcctcatct gctgcccgag
     1081 gagcctttca ggggaattag gcttcccgac tctccttccc tgagccctgg cctctctgtc
     1141 aactgtcttc tgagctgcac tttcctcggg tgccttatgc agtgactaca ccagcacaga
     1201 cctggcaggg atttgtcttg tccctgctcc tccctcagta gtcagctttc tcctctacaa
     1261 gagggaagga gaggaaggga caaggacttt aaaaaaaaaa aaaacacatg ggaaaagaaa
     1321 aaataacaaa gctgtctttc cttttctaat ggtggcttgg tctgattttg tctggaagca
     1381 atgggactgt tttccctgaa aacaaatgga ctcatgtgct tgggatcatg ggccaacctg
     1441 ggctcactgt actggggatt ttccacaggc accctgtact tcactccctc cctctacagg
     1501 ctccatgtgt attgggtctc cacaaccttg cctttcatgc ttgggcttcc taagtccccc
     1561 agtggctcta ctctaggtga gctgtgttct gggtgggagc acagaagtct cccaagtcct
     1621 cttccggtga gctcctggtg ttcttcacaa ccgagtcatg accctgtgcc tgctacgact
     1681 catgtgtgat agcaccttgt gccctctggg ggccctccct agcctctctt cagacttctg
     1741 aatgtacagt aagtggggtt ggttgagact tgggggatga ggaggggacg aagacaagta
     1801 agtcaagtcc tgcccagcct ctaccagtct tgtgagaggg tagatgtctg cttgttttgg
     1861 cttctctcct cccaagggac tctgcccctc tgccttagct tggcccctgc ctatcttcag
     1921 caatactcac ctcttaaaac gcttagggtc ttttggaaga aatcagtcct gaactatgtc
     1981 ctgcaggcag gactgccgag ccttctttct gagggttctt cccacaatgg agtctaggtc
     2041 cctttctact tgtcctgggg gctccacggg tgtgtcactg cagtggcacc taagaagccg
     2101 ctgtggaaac ctggaccctg tgttttgtca gtactgcgag agctcaccaa gctggccagt
     2161 ctcctgcccc tgggaactgg ggccctcctg agactaccct catgctgtca gccctagagc
     2221 caccaagtgg taatgtcagc caccggggtt ttgtaaactt cctggtatta ttttcctcca
     2281 tgtacagatg tatatgtcat gtctcaattt ttgtgcttaa ataaaaagat tttcggacag
     2341 caaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
