LOCUS       NM_001359978           10159 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus strawberry notch 1 (Sbno1), transcript variant 1,
            mRNA.
ACCESSION   NM_001359978 XM_006530317
VERSION     NM_001359978.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Zhang,Y., Zhu,Z., Li,Z., Feng,J., Long,J., Deng,Y., Ahmed,W.,
            Khan,A.A., Huang,S., Fu,Q. and Chen,L.
  TITLE     Sbno1 mediates cell-cell communication between neural stem cells
            and microglia through small extracellular vesicles
  JOURNAL   Cell Biosci 14 (1), 125 (2024)
   PUBMED   39343943
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 10159)
  AUTHORS   Modzelewski,A.J., Shao,W., Chen,J., Lee,A., Qi,X., Noon,M.,
            Tjokro,K., Sales,G., Biton,A., Anand,A., Speed,T.P., Xuan,Z.,
            Wang,T., Risso,D. and He,L.
  TITLE     A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform
            essential for development
  JOURNAL   Cell 184 (22), 5541-5558 (2021)
   PUBMED   34644528
REFERENCE   4  (bases 1 to 10159)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10159)
  AUTHORS   Shen,C., Yu,J., Zhang,X., Liu,C.C., Guo,Y.S., Zhu,J.W., Zhang,K.,
            Yu,Y., Gao,T.T., Yang,S.M., Li,H., Zheng,B. and Huang,X.Y.
  TITLE     Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial
            stem cells via the noncanonical Wnt pathway in mice
  JOURNAL   Asian J Androl 21 (4), 345-350 (2019)
   PUBMED   30198493
  REMARK    GeneRIF: we performed Sbno1 gene knockdown in mice to study the
            effects of SBNO1 on neonatal testicular and spermatogonial stem
            cell development. Our results revealed that SBNO1 is required for
            neonatal testicular and SSC development in mice.
REFERENCE   6  (bases 1 to 10159)
  AUTHORS   Jerome-Majewska,L.A., Achkar,T., Luo,L., Lupu,F. and Lacy,E.
  TITLE     The trafficking protein Tmed2/p24beta(1) is required for
            morphogenesis of the mouse embryo and placenta
  JOURNAL   Dev Biol 341 (1), 154-166 (2010)
   PUBMED   20178780
REFERENCE   7  (bases 1 to 10159)
  AUTHORS   Baba,K., Dekimoto,H., Muraoka,D., Agata,K., Terashima,T. and
            Katsuyama,Y.
  TITLE     A mouse homologue of Strawberry Notch is transcriptionally
            regulated by Reelin signal
  JOURNAL   Biochem Biophys Res Commun 350 (4), 842-849 (2006)
   PUBMED   17045962
REFERENCE   8  (bases 1 to 10159)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   9  (bases 1 to 10159)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 10159)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127339.3.
            
            On Jan 24, 2018 this sequence version replaced XM_006530317.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC138418.1, SRR1660811.245436.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC127339.3         167909-168064       c
            157-285             AC127339.3         156861-156989       c
            286-390             AC127339.3         155647-155751       c
            391-703             AC127339.3         152532-152844       c
            704-804             AC127339.3         150939-151039       c
            805-901             AC127339.3         149553-149649       c
            902-1062            AC127339.3         148031-148191       c
            1063-1196           AC127339.3         146651-146784       c
            1197-1287           AC127339.3         146422-146512       c
            1288-1449           AC127339.3         144524-144685       c
            1450-1585           AC127339.3         143603-143738       c
            1586-1691           AC127339.3         143424-143529       c
            1692-1845           AC127339.3         143170-143323       c
            1846-2028           AC127339.3         142466-142648       c
            2029-2126           AC127339.3         140874-140971       c
            2127-2278           AC127339.3         138357-138508       c
            2279-2473           AC127339.3         136263-136457       c
            2474-2641           AC127339.3         135301-135468       c
            2642-2817           AC127339.3         135036-135211       c
            2818-2949           AC127339.3         134384-134515       c
            2950-3085           AC127339.3         130987-131122       c
            3086-3198           AC127339.3         129939-130051       c
            3199-3270           AC127339.3         129781-129852       c
            3271-3370           AC127339.3         129292-129391       c
            3371-3445           AC127339.3         126929-127003       c
            3446-3592           AC127339.3         124321-124467       c
            3593-3687           AC127339.3         124145-124239       c
            3688-3780           AC127339.3         123971-124063       c
            3781-3918           AC127339.3         121747-121884       c
            3919-3995           AC127339.3         120932-121008       c
            3996-4189           AC127339.3         118560-118753       c
            4190-10159          AC127339.3         111164-117133       c
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 63.54 cM"
     gene            1..10159
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="strawberry notch 1"
                     /db_xref="GeneID:243272"
                     /db_xref="MGI:MGI:2384298"
     exon            1..156
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..4332
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="isoform a is encoded by transcript variant 1;
                     protein strawberry notch homolog 1; sno, strawberry notch
                     homolog 1"
                     /codon_start=1
                     /product="protein strawberry notch homolog 1 isoform a"
                     /protein_id="NP_001346907.1"
                     /db_xref="CCDS:CCDS39280.1"
                     /db_xref="GeneID:243272"
                     /db_xref="MGI:MGI:2384298"
                     /translation="MVEPGQDLLLAALSESGISPNDLFDVDGGDAGLATPTPPSVQQS
                     VPLSALELGLETEAAVPVKQEPEPMSTPALLNVRQQPPSTTTFVLNQINQLPTLGSTI
                     VMTKTPPATTNRQTITLTKFIQTTANTRPSVSAPAVRNAMPAAPSKDQVQLKDLLKNN
                     SLNELMKLKPPANIAQPVATAATDVSNGAVKKESSNKEVARIWINDMKMRSFSPTMKV
                     PVVKEEDEPEEEDEEEMGHAETYAEYMPIKLKIGLRHPDAVVETSSLSSVTPPDVWYK
                     TSISEETIDNGWLSALQLEAVTYAAQQHETFLPNGDRAGFLIGDGAGVGKGRTIAGII
                     YENYLLSRKRALWFSVSNDLKYDAERDLRDIGAKNILVHSLNKFKYGKISSKHNGSVK
                     KGVIFATYSSLIGESQSGGKYKTRLKQLLHWCGDDFDGVIVFDECHKAKNLCPVGSSK
                     PTKTGLAVLELQNKLPKARVVYASATGASEPRNMAYMNRLGIWGEGTPFREFSDFIQA
                     VERRGVGAMEIVAMDMKLRGMYIARQLSFTGVTFKIEEVLLSQSYVKMYNKAVKLWVI
                     ARERFQQAADLIDAEQRMKKSMWGQFWSAHQRFFKYLCIASKVKRVVQLAREEIKNGK
                     CVVIGLQSTGEARTLEALEEGGGELNDFVSTAKGVLQSLIEKHFPAPDRKKLYSLLGI
                     DLTAPSNNSSPRDSPCKENKIKKRKGEEITREAKKARKVGGLTGSSSDDSGSESVSDN
                     DESDYESSKNMSSGDDDDFNPFRDESSEDNEDDPWLIRKDHKKSKDKKKKKSIDPDSI
                     QSALLASGLGSKRPSFSSAPVISPASNSAPANSNSNSNSSLVTSQDAVERAQQMKKDL
                     LDKLEKLAEDLPPNTLDELIDELGGPENVAEMTGRKGRVVSNDDGSISYESRSELDVP
                     VEILNITEKQRFMDGDKNIAIISEAASSGISLQADRRAKNQRRRVHMTLELPWSADRA
                     IQQFGRTHRSNQVTAPEYVFLISELAGEQRFASIVAKRLESLGALTHGDRRATESRDL
                     SRFNFDNKYGRNALEIVMKSIVNLDSPMVSPPPDYPGEFFKDVRQGLIGVGLINVEDR
                     SGILTLDKDYNNIGKFLNRILGMEVHQQNALFQYFADTLTAVVQNAKKNGRYDMGILD
                     LGSGDEKVRKSDVKKFLTPGYSTSGHVELYTISVERGMSWEEATKIWAELTGPDDGFY
                     LSLQIRNNKKTAILVKEVNPKKKLFLIYRPNTGKQLKLEIYADLKKKYKKVVSDDALV
                     HWLDQYNSSADTCTHAYWRGNCKKASLGLVCEIGLRCRTYYVLCGSVLSVWTKVEGVL
                     ASVSGTNVKMQIVRLRTEDGQRIVGLIIPANCVSPLVNLLSTSDQSQQLAVQQKQLWQ
                     QHHPQSITNLSNL"
     exon            157..285
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            286..390
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            391..703
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            704..804
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            805..901
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1062
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1196
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1287
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1449
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1585
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1691
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1845
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2028
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2126
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2278
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2473
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2641
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2817
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..2949
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3085
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3198
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3270
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3370
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3445
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3446..3592
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3593..3687
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3780
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3918
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3919..3995
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            3996..4189
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     exon            4190..10159
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5678..5683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="hexamer: AATAAA"
     polyA_site      5699
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="major polyA site"
     regulatory      10134..10139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="hexamer: AATAAA"
     polyA_site      10153
                     /gene="Sbno1"
                     /gene_synonym="9330180L10Rik; mSno1; Sbno; sno"
                     /note="major polyA site"
ORIGIN      
        1 gtcgggggga ggggcttcaa gatggcggcg gggaggtagg cagagccaga cgccgcttct
       61 gccgccgccg ccgccgcctc cgctcctgtc gcctccggct cctcacccgt tcgcggaccc
      121 ggagaagcta tccttgggtc ggccccggcg gggaaaatgg tggaaccagg gcaagattta
      181 ctgcttgctg ccttgagtga gagtgggatt agtccgaatg acctctttga tgttgatggt
      241 ggagatgctg gccttgccac ccctacgccc ccttcagttc aacagtcggt gccacttagt
      301 gcattagaac tagggttgga gacagaagca gcagttcctg tcaaacaaga gccagagccg
      361 atgtccactc ccgcactgtt aaatgtgagg cagcagcctc catccactac aacgtttgtg
      421 ctgaatcaaa taaatcaact tccaactttg ggatctacaa ttgtaatgac taaaacacca
      481 cctgcaacaa cgaataggca aaccatcact ttgacaaagt tcatccagac cactgcaaac
      541 acacgccctt cagtttcagc accagcagta cgaaatgcca tgcccgctgc tccttcaaaa
      601 gaccaggttc agctaaagga tttactcaaa aataatagtt taaacgaact catgaaactg
      661 aagccacccg ccaatattgc tcagccagtt gcaactgcag ctactgatgt cagtaatggt
      721 gcagttaaga aggagtcttc taataaagaa gtggcgagga tatggataaa tgacatgaag
      781 atgaggagct tttcccccac catgaaggta cctgttgtta aagaggaaga tgaaccagaa
      841 gaagaagatg aggaagaaat gggtcatgca gagacctacg cagagtatat gccaataaaa
      901 ttaaaaattg gcctgcgtca ccctgatgca gtggtggaga ctagctctct gtccagtgtc
      961 actcctcctg atgtttggta caaaacctcc atctctgaag agaccattga taatggctgg
     1021 ctgtctgcat tgcagcttga ggcagttacc tatgcggccc agcaacatga aacattccta
     1081 cctaatggag accgtgctgg cttcttaata ggcgatggtg ctggtgtggg taaaggccgg
     1141 acaatagcag ggatcatata tgaaaactat ttgttgagta gaaaaagagc attgtggttt
     1201 agtgtatcaa atgacttaaa atatgatgct gaaagagatt taagggatat tggagcaaaa
     1261 aacattttgg tccattcgtt aaataagttt aaatatggta agatttcttc caaacacaat
     1321 gggagtgtga aaaaaggtgt tatttttgct acctattctt cccttattgg tgaaagccag
     1381 tctggtggta aatataagac caggttaaaa cagcttctac actggtgtgg tgacgacttc
     1441 gatggagtga tagtgtttga tgaatgtcac aaagcaaaaa acttatgtcc tgttggttct
     1501 tcaaagccaa ccaagacagg cttggctgtt ttagagcttc agaacaaatt gccaaaagcc
     1561 agagttgttt atgccagtgc cactggtgct tctgaaccac gcaacatggc ctacatgaac
     1621 cgtcttggaa tatggggtga gggaactcca ttcagagaat ttagtgattt tatccaagct
     1681 gtggaacgca gaggtgttgg tgccatggaa atcgttgcta tggatatgaa gcttagagga
     1741 atgtacattg cacggcagct gagctttact ggagtgacct tcaaaattga ggaagttctt
     1801 ctttctcaaa gctatgtgaa aatgtataac aaagcagtca agctgtgggt tatcgctaga
     1861 gagcgatttc aacaagctgc agatttgatt gatgctgagc agagaatgaa gaaatcgatg
     1921 tggggtcagt tctggtctgc tcaccagagg ttttttaagt acctgtgtat agcatccaaa
     1981 gtgaaaaggg tggtgcagct ggctcgggaa gagatcaaga acgggaagtg tgttgtaatt
     2041 ggtcttcagt ctacaggaga agccagaact ttagaggcct tggaagaggg aggaggagaa
     2101 ctaaatgatt ttgtttcaac tgccaaaggg gtattgcagt cactgattga aaaacacttc
     2161 cctgctccag acaggaagaa actttatagt ttgctaggaa tcgatttgac agccccaagt
     2221 aacaacagtt caccaagaga tagtccttgt aaagaaaata aaataaagaa gcggaaaggt
     2281 gaagaaatca ctcgagaagc caaaaaagca cgaaaagttg gtggcctcac aggaagcagc
     2341 tctgacgaca gtggaagtga gtctgtgtct gataatgacg aaagcgacta tgagagctct
     2401 aaaaacatga gctctgggga tgatgacgat ttcaatccct tcagagatga gtccagtgaa
     2461 gacaatgagg atgatccgtg gttaattagg aaagaccata agaaaagcaa agataaaaaa
     2521 aagaagaaaa gtatagatcc agattccatt caaagtgcct tgttagcatc aggtcttgga
     2581 tcaaagcggc ctagtttctc ctctgcacca gtcatctcgc ctgcttccaa cagtgcacca
     2641 gccaacagta acagcaacag caacagcagc cttgtaacaa gtcaagatgc tgtcgaaagg
     2701 gcgcaacaga tgaagaaaga cctgcttgat aaactagaaa aattggctga agacctccct
     2761 cctaacaccc tggatgaact tatcgatgaa cttggtggtc ctgaaaatgt ggctgagatg
     2821 actggccgca aagggcgggt agtgagcaat gatgacggaa gcatatccta cgagtcgagg
     2881 tctgaacttg atgtgcctgt ggagattcta aacatcacag agaagcaaag gttcatggat
     2941 ggagacaaga atattgctat tatctcagaa gctgccagct caggtatttc attacaagca
     3001 gatcggagag ctaaaaacca aaggcgaaga gttcacatga ctttagaatt accttggagt
     3061 gctgataggg cgattcagca gtttggaaga acacacaggt caaaccaagt gactgctcca
     3121 gagtatgttt ttttgatttc ggaactggct ggagaacaga gatttgcatc tattgttgct
     3181 aaaagacttg agagtttggg ggcacttaca catggtgaca gaagagcaac agaatctaga
     3241 gatctgagca gattcaactt tgacaacaag tatggaagaa atgctttaga aattgttatg
     3301 aaatccattg taaacttgga ttctcctatg gtatcaccac ctccagacta ccctggagaa
     3361 ttctttaaag acgttcggca aggactgata ggagtgggct tgattaatgt agaggatcgc
     3421 tcaggaattc ttactcttga taaagactat aacaatatag gaaagttctt aaacagaatc
     3481 ctgggcatgg aggtgcatca gcagaatgcg ctgtttcagt attttgcaga cacacttact
     3541 gcagttgttc agaatgccaa gaagaacgga agatacgaca tgggaatcct agatctgggt
     3601 tctggagatg agaaggtgag gaagagtgat gtcaagaagt ttctaactcc agggtattca
     3661 acctccggcc atgtagagtt atacacgatt agtgtggaga ggggaatgtc ctgggaggaa
     3721 gcaaccaaga tttgggcaga gctgactgga ccagatgatg gcttttactt atcattgcag
     3781 ataagaaaca acaaaaaaac tgcaatctta gttaaagaag taaaccctaa gaagaaacta
     3841 tttttaattt accgaccaaa cactgggaag caactcaaat tagaaattta tgctgacctg
     3901 aaaaagaaat ataagaaggt agtttctgat gacgccctgg tacactggct ggatcagtat
     3961 aactcctctg cagacacctg tactcacgca tactggcgtg gcaattgcaa aaaagcaagt
     4021 ttaggattgg tatgtgagat tggtctccgc tgccgcacgt actatgtact gtgtggctca
     4081 gtactgagcg tctggacaaa agtggaaggt gttctggcct ctgtcagcgg cacaaatgtg
     4141 aaaatgcaga tagtgcggct aaggaccgaa gatgggcagc ggattgtagg tttgatcatt
     4201 ccagcaaatt gtgtgtctcc tcttgtaaat ctcctgtcaa cttcagacca gtctcagcag
     4261 ctcgcagtgc aacaaaaaca gctgtggcaa cagcatcacc cacagagcat caccaacttg
     4321 agcaacctgt gaggagcagc ggcgtgcaca ggcggtgctg agtgctgtga gagcatacta
     4381 cttgcataaa aatcagggac agtttccaaa gaatcctttt tatttttaac ttcagttgtg
     4441 ctctctagtt aacttggggg ggtaaggaca aacctggaaa aaggcagcaa agctgatggc
     4501 gcaaacagag tagaggggac acgtgtcttt cctccagctt ccttgaggct gcctgtgcgc
     4561 ttttactttg gtgagcagag cggatgtgtc tgtgtgtgtg cgtgtatgtg tcagtctgtc
     4621 tgtgagtttg ttacatgcta caaggccaca gttggttgaa aaggcttgga acttcccaga
     4681 ctggctttcc ctttcctttc tacagtgctc agggtaacac agtacatcct tgtccttact
     4741 gtgggagttc cccggatgca tgggtttcaa gtctaaatac tgagtatgta tattggtttc
     4801 ttttccaact tacacaatgt ttcctacagc atgaagtgaa gggttgcgtg ccatgcattc
     4861 aggttcattt gtagcttttg ttgacgatgc atgtctttga aagcatgtgt aatcaagatt
     4921 cccacagaag tcacatggag agaaacattg gagatgtctg gcattgggta ctgcatttct
     4981 agtgttgata cctgggtgtt gcttctaacc ctcagccctg cccgctcccc ctggcccaag
     5041 tttcttgttg aagtagtaag tacatacttt tttgtgagaa agctctttaa aagttcattt
     5101 taatatgtta atagtattcc taatttgtgc tgcaaaaatg gctatgagcc ttatcatata
     5161 agttccattt tccttaactt aagcatagtt ttaggaagac atatgattgg cttctgtctt
     5221 gcacacaatc ttttttactt cattatctga atctgctttg tcattcaaac cagaaaccgt
     5281 acttgagttg taaactagac agtgagaaaa cacttggctt ctgctgtatg ttgttgtcat
     5341 cgttacccag taacttgaat attgtttaat gtgctgtaag acattataga gattatctcc
     5401 agctgtttaa aatggtaatg tacaaatgtg aatagacact tatctataac atgggtaagt
     5461 tttgttttgc ctataataga tgtttataaa aaaattcagt gaggggacaa ttggtctttt
     5521 tggttttttt ttttctccca atcctctcct tcccccattt tttttctatc ttcttctttt
     5581 tttttttttt tttgcttgca caggttgcac tattgaaaaa ttgagattgt ataaatctgg
     5641 tttaaaacta caagataaca aaatcttgta gatgttgaat aaaaaagtta ttatactaac
     5701 gaagtggact cttgtttagg ccctccctgg ggactccagg cataggatag ctggcctctg
     5761 ttacttggag ttgccttagc cttccaggta gccttagagt ctgactgagc cccttcctga
     5821 agtctgtgga ggtcttaggg atggggtaac tgatggtact tttgaatgtt tttcaagttg
     5881 aattgttttc tttgtagctt tttaatcttc tcaaagagct gggtgaggtg tgtttagtgg
     5941 cagagacttg atggagcgct tgggctctca ttctctcaag tttctaggat ttagtttgct
     6001 gtaaaactgc attggattta tttattctta gtgcctcaaa gaacaaatgg tgaatggttt
     6061 ctgttcttgg gcacattttg cccagtggat gcaactttta tcctcattcc ttgtatctca
     6121 ggcagtgatc agcaagcagt ccccctttgt ggcccctgag aatagaatgg gtcctccttt
     6181 acctagcctc tgagcttctg tgtttgaagt agcagactgt tcagcttcct tgggcatggt
     6241 aaagtaaaag cctgaggaag agctgtgtaa ctgaacttgc tttcttcagg aaaccaagtc
     6301 acagaggatg aggaatgaga acgattctgt gtcctaaggt gccagccccc tcgttctgtg
     6361 tgtggatatg tgtttatgca ggtcctgctt tatctggtgt ttccaaagca gcacctgcag
     6421 gatttctcta gtcttcttag tgaacaggtc attcatggtg atgacgctgt gacacttgct
     6481 cctggagcag atgaggatct gggtgtcctt ttcatcaaag aacgtttcat tgcgttttaa
     6541 tgttgagtga ggaaaagtcc acagatacca ggcatctgac aaccctttca aaacagaatt
     6601 aaaggtaggt agtgtggtgg gacagacaga aagctggtca gcctttaccc actgttgggg
     6661 ggtgtgagac atctggaatt ggtgcccagc ctctctgcac tctgccccta caaaagtcac
     6721 tcgttggcag tcatgtcttt ttcagccact gtcatgtaaa gtaggttcag tttcctgcag
     6781 ggtctgagtg ccacacatgc acccaggacc tcacttaact ctgtctccct agcctctgag
     6841 atgccttctc ccacttgagc ggtgcagctc tgagcagcag acccgagtgt tggttccatc
     6901 agtgttttca gagtttacag tagtttgtgc tgaaaatcac tcctaggttc cagctgctca
     6961 aatatacttg ctggagagga aggccatgtt cctcccctag caacctctgt gctgcaggct
     7021 tgccctctct tgttccagtc agattgttgg tggtggtgca gtcagaaaag cagtgaaagt
     7081 atttctataa atgcaagttg gtgagaaagc taggcaggct aagcagtttg ctctctttac
     7141 tactaaattg caggtctgct ttcagtgtct tctttttttc tgtgtgtata gactagcaag
     7201 aacttgtatc caaagcactg tagatgctat aggtatgtac atacgtgtat ttattcttac
     7261 tcagcactta aaccttttga gttgcaacag taaatcttgt aatttgctct gtgtgtgtag
     7321 agagaagtta taaactaccc agctccagag gaattaagca ttttggagcc acagtttggc
     7381 tgtagccttt ttatttgctg gtttctgtac tatgtcatca gaacaacttc tgagatagga
     7441 gagaaggggt aggaggacct accatttaca gtttgtcaca ggagggagcc ccatcctgtc
     7501 ctcttccata ttagcactag ggccactgtg acttgcattt gaaagcaggt gaagaggtat
     7561 ttatcctatg agaccttacc cagggatcct ggtcttaaag aaggaccctt gtgggctctt
     7621 gcacttccta ggaatggagc ttcagaaaag ctgctattaa gccaggatcc acacattgaa
     7681 tgttcagtta gttggggcat ctctacggca acagaaactg ttgtgatggt ttgtttgtta
     7741 agagtcagtc ctgtgggtgt gggctctggt gaacaacata cacaacacag gcactttggc
     7801 cctctagtgc tgaagtgaac caagcattcc atgtcatggc tgcagctgag tagacagaaa
     7861 ttctttgcat gaaaatccct cacaaggcta gctgtctaaa tccaatgagt cctataaggg
     7921 aaagaaacag ggaggtgcaa gcaggtgcag tcttctccgt gcccatcttc tcttctatgc
     7981 tatccttaca catgtacata acgagtgtat agacaagtgt gagttcttca acattgcgag
     8041 tgtaaatctt gtaaatcaag accctgttca tttggggttt tttgctcccc ctattccttt
     8101 ccctcctatt tattcttttt agactcctac ctggggcatc ttagtacttt tttttttttt
     8161 ttttaattta ggaaactgtg gaaaagttct tagctgagta aatagttttg tgtttaagat
     8221 ttcttctttg cctcacagct gtgggggtct tgttacccac atcgccaaca cacactgctg
     8281 tgctgtcatg gggtgcatct aagcaccacc ccttatcctg tcaccaggtg acttgttatg
     8341 ctcatttttg ttctctggaa gctatagtga gcctgatggc tttcatattt gaaaaaagaa
     8401 gaaagaaaag aaaatgtctc cctgaacttc cttaagctcc tctagcatct tcagtcagtc
     8461 agatgcagct cctcagaagc ctgactcatt cttgctttca gttgaggctg ctccagtccc
     8521 tcagctcctg ctgtttgccg gatgggaaac ccttgcaggt agacagtagc tcatagcctg
     8581 gctgcacgtt acagtgtgta taaggagttg gatatctctg accccacagg tgacagtcag
     8641 agatttactt tacaacaaac gtccgtaatc tgttttctat actgctggtc aaaaatattt
     8701 ataggaggac aagagatgag cttatagtac ctgacaggag acagttgtca tccaagtgat
     8761 gacaagagtc ctactgtgga agctaccacg agcacacaag gcaggttcag ttttgggggc
     8821 tcatttctgg ctggataagc tataagcaga gatgctgagc tctggagaat tggtgttggt
     8881 gcctaccctc acccaggaca caggagtgga gtttattcac aggccattac accctcaaaa
     8941 aaatgtcctc tgtaattctc aggaaagatt tcaattaaca ttcagattgg tagatctgat
     9001 gacatgttga aaattgtcta catttggctg tctttgtaag ttagtagtca agaatgaggg
     9061 tctgtcaccg attgttccct cagaaatttc ctcctcacac caacttcctt cctctggtcc
     9121 ctgagatata ggtgacctgt ggcctgcaac agcagccaac tatgtactat ctagggagat
     9181 gtgacagagc acagttaggc caggaaatct acagcaaaca gttaattatg atgtacaccc
     9241 atttcatgca gagcaagagg agatggaatg gaggctgctt tttgcactgg aggctccctc
     9301 aggactgagt cagcatgcca gtctcctggg gagagttagt gtggcctttc tgattctggc
     9361 ctcttaaccc agaccccact caggcgtcta gtaggagctt gctgctgttg cagtgactgg
     9421 ggcactgaag aggtaaagaa gcttgaacca ttcctggctc cactttgtgg gactgagggg
     9481 ccttttcctt ttcttcttta acatagcaca gtacagcctc gccactaaac tagtcaccct
     9541 gttcatagtt tttactgaat ttgcaaacta ctcttacttc ctctcataac cacctttatc
     9601 atcaaccacg aggggtggga gaggtgcacc gtgtctctgc aataaacttg tgccgggttc
     9661 tacctccttg tgcaaagggt attttataca cgggctctgg gcttccttct tgatctgaca
     9721 tggtgcatct ggagacatct gaaggagtcc aaataccttt tgaagcaaag tcagatgtaa
     9781 agtctggggg gcttctgtct tcagttccct tttgtactgc tgcgctgcat aggactgtcc
     9841 atctgaatga agagtgcacg ctgttccccc gatgccacgt tgctgccttc agcctctccc
     9901 aggaacctgc tcccaagtgt ccagaatgca gtgctgccat cattttgata tgtaactttc
     9961 aaatccagtc tcatgcaata caagtgtgtc agcttcactg agcagggttc atttgcccac
    10021 aagtagaaat gctcgatcct cacatggaaa gtactctatg tatttaggtt tttgttgtgg
    10081 ttttgttttt tcatttttgt ttggattttg tttctgtttt tgtaaagttc ttaaataaaa
    10141 ttaaacaaat gcactttga
//
