LOCUS       NM_001146329            2144 bp    mRNA    linear   ROD 03-AUG-2024
DEFINITION  Mus musculus SH3-binding domain kinase family, member 2 (Sbk2),
            mRNA.
ACCESSION   NM_001146329 XM_355841
VERSION     NM_001146329.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157563.12.
            
            On Feb 15, 2019 this sequence version replaced NM_001146329.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784643.877895.1, BY708898.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation, longest
                                                protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AC157563.12        131203-131308       c
            107-361             AC157563.12        129833-130087       c
            362-564             AC157563.12        125732-125934       c
            565-2144            AC157563.12        123036-124615       c
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 2.87 cM"
     gene            1..2144
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /note="SH3-binding domain kinase family, member 2"
                     /db_xref="GeneID:381836"
                     /db_xref="MGI:MGI:2685925"
     exon            1..106
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /inference="alignment:Splign:2.1.0"
     exon            107..361
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..1197
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /EC_number="2.7.11.1"
                     /note="sugen kinase 69; SH3 domain-binding kinase family
                     member 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase SBK2"
                     /protein_id="NP_001139801.1"
                     /db_xref="CCDS:CCDS51980.1"
                     /db_xref="GeneID:381836"
                     /db_xref="MGI:MGI:2685925"
                     /translation="MPGKQSEDKPMEVSTVEDGGDEGLGGLTVEELQQGQEAALALED
                     MMALSAQTLVQTEVEELYEEVRPLGQGRFGRVLLVTHRQKGTPLALKQLPKQSTSLRG
                     FLYEFCVGLSLGTHSAIVTAYGIGIESANSYSFLTEPVLHGDLITFIQPKVGLPQPAA
                     QRCAAQLASALEHIHSHGLVYRDLKPENVLVCDPACQRVKLTDFGHTRPRGTLLRLTG
                     PPIPYTAPELCAPPPLPEGLPIQPSLDAWALGVLIFCLLTGYFPWDQPLVEVDPFFED
                     FLIWQASGQPQDRPQPWYSLSPAADTLLWGLLDPHPRKRNPVGSIKSYLGQPWKQREG
                     EAEELATELREDGWRGGQEAAKGEQPAC"
     exon            362..564
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /inference="alignment:Splign:2.1.0"
     exon            565..2144
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2121..2126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /note="hexamer: AATAAA"
     polyA_site      2144
                     /gene="Sbk2"
                     /gene_synonym="Gm1079; Sgk069"
                     /note="major polyA site"
ORIGIN      
        1 agtctgatgc tggcaggaac ccccgaagtg caaggcgcta actgcagtag gcagaaaggg
       61 atcagacaag ccttggagaa ggcgaagtct ggagctctga gcccagaaat gccaggcaaa
      121 cagtcagaag acaagccaat ggaagtgtca actgtggagg atggaggtga tgaaggcctg
      181 ggaggcctaa cggtagagga gctgcagcag gggcaggagg ctgctctggc cttggaggac
      241 atgatggctc tgagtgccca gaccctggtc caaactgagg tggaagagct gtacgaggaa
      301 gtgcgtcccc tgggccaggg ccggttcggc cgtgtcctgc tggttactca ccgccagaaa
      361 ggaacacccc tggcgctgaa gcagctcccg aagcaatcca cttccctccg tggcttcctg
      421 tatgagttct gcgtgggcct ctcacttggc acacattctg ccatcgtgac agcctatggc
      481 attggcattg agtcagccaa ttcctacagc ttcctgacag aacctgtcct ccatggggac
      541 ctaatcacct tcatccagcc caaggtgggt ctcccacagc cagcagccca gcgctgtgca
      601 gcccaactgg cctctgccct ggaacacatt cactcgcatg gcctggtgta ccgggacttg
      661 aagccagaga atgtgctggt gtgtgaccca gcctgccagc gcgtcaagct gacagacttc
      721 ggccacaccc ggccccgagg gaccctgctc cgactcactg ggccacctat cccctacact
      781 gcccctgagc tctgtgcccc accacccctc cccgagggac tacccatcca accttccctg
      841 gatgcctggg ccctaggggt cctgatcttc tgcctcctta ctggctactt cccctgggac
      901 cagcccctgg tggaggttga ccctttcttt gaggactttc tcatctggca ggcttctggt
      961 cagccccaag accggccaca gccctggtat agcctgtcac ctgcagcaga cactctccta
     1021 tgggggctgc tagatcctca cccccggaag aggaaccctg tgggctccat caagagctat
     1081 ctaggccaac cctggaagca gagggaaggg gaggctgagg aattggcaac ggagctgaga
     1141 gaagatgggt ggagaggagg gcaggaggct gcaaaggggg agcaacctgc atgctgagac
     1201 ctgcttagcc tgagctgcct ggctgtggcc tcttcttggt tttcgctcta tctatctctc
     1261 atctgtcctg tgtgttatct gtgcccctcc tttatgctgt gtgtattcac gaatgtgtgc
     1321 acgtgtgctt gtgtgcatgc atgtatgtgt ttgtatgtgt gtatgtgtgc atgcatttgt
     1381 gtgtatgtgt gtgtacatgc acatatgcgt gtatatacac atacctgtgt atgcacatat
     1441 atgtgtgtat atggggtgtg tgtgtaaagt gctgtgtgta aatcagagga cttttagcag
     1501 tcagtcctct ccttccacct tggagcccag ccatggaatt cttataagcc ttcaagtgcc
     1561 tttagctacg ggagccatct tgctggctct ggtctatata actctgaatc cccctgcctt
     1621 agcttcctga gtcctggaat agcaggcatg caccatgctc tgctgtccgg catagctctg
     1681 caccatccca cccacctcag ctaccttccc cacaaaccca ggtaaaatgg ccacctgctc
     1741 tgaacttcac aaccctaggc ttctctgtac ttctatgtac ttttgtgtcc tttactaact
     1801 gcccgcttat tttctgagca cccagggggt ggtttttggt ccctgaagga ctgaccctac
     1861 atctctgagc aatgcccagc ctgcagcagc aaccaaatga atagacacat ctggtcagca
     1921 ttgctccaca ccatgtgggc ctctcgcact ctgtcactgt cctctggctc tctcattctg
     1981 tctctgtcct cctgtctccc atcttctctc atgtggcttc gctccactca aatctgctct
     2041 ggcttttgtt gctgcttact cacctgcatt ctgggattcc accctttgag tgaggtacac
     2101 ttgcccagct gctctctgca aataaagttc ccctggcaac tgaa
//
