LOCUS       NM_133689               1511 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus stabilizer of axonemal microtubules 4 (Saxo4),
            transcript variant 2, mRNA.
ACCESSION   NM_133689
VERSION     NM_133689.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Nguyen,T.T.T., Tokuhiro,K., Shimada,K., Wang,H., Mashiko,D.,
            Tonai,S., Kiyozumi,D. and Ikawa,M.
  TITLE     Gene-deficient mouse model established by CRISPR/Cas9 system
            reveals 15 reproductive organ-enriched genes dispensable for male
            fertility
  JOURNAL   Front Cell Dev Biol 12, 1411162 (2024)
   PUBMED   38835510
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1511)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   4  (bases 1 to 1511)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1511)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1511)
  AUTHORS   Danielson,P.E., Sautkulis,L.N., Foye,P.E., Hedlund,P.B. and
            Carson,M.J.
  TITLE     A novel mRNA expressed along brain ventricles
  JOURNAL   Brain Res Gene Expr Patterns 1 (2), 83-88 (2002)
   PUBMED   15018803
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK005821.1, AY032666.1 and BX630729.1.
            
            On Jan 19, 2019 this sequence version replaced NM_133689.1.
            
            Transcript Variant: This variant (2) differs in the 3' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005821.1, AY032666.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-556               AK005821.1         2-557
            557-745             AY032666.1         526-714
            746-1507            AK005821.1         748-1509
            1508-1511           BX630729.1         3-6                 c
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 6.6 cM"
     gene            1..1511
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /note="stabilizer of axonemal microtubules 4"
                     /db_xref="GeneID:67752"
                     /db_xref="MGI:MGI:1915002"
     exon            1..101
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            102..237
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..1412
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /note="protein phosphatase 1, regulatory subunit 32"
                     /codon_start=1
                     /product="stabilizer of axonemal microtubules 4"
                     /protein_id="NP_598450.1"
                     /db_xref="CCDS:CCDS29577.1"
                     /db_xref="GeneID:67752"
                     /db_xref="MGI:MGI:1915002"
                     /translation="MMGKLPLGVVSPYVKMSSGGCSDPLKFYATSYCTAYGREEFKPR
                     LGSHVGTGYKSNYRPLVSYQPHLDTLDNPAIGQQIRDTSKSVTSQSYSPLEVPDGKQP
                     LPWNLHQTTSSYGREKLNPGPHSKEVRKVHFDTQDHGPQTITGLEPKEVPLIHQQQGK
                     GSTEWENSHYGPRFMTSEYNSKYIKESPNHPDLLLKKTIGSKEETGFTEESTKNPIVF
                     QPPSQAFPGDPVLHPGRSITKSDYLPVTHPQGSDFLPVLSRGSDRDTGFSRVNERTLN
                     PRVPTPAPQSASMSHRSYQPPQRMQQTNVALLGRESVGNKEPTGFTLNNPSYVRSSYE
                     QDRDQRYLTTYNQGYFENIPKGLDREGWTRGGIQPQKAGAYALSELNNHTLMDSTPNP
                     TETLRHLHPHVGRTLASVDPFYRDMPHSSRYPASS"
     exon            238..355
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            356..509
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            510..638
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            639..702
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            703..815
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            816..875
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            876..962
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1076
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1171
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1342
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1421
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1511
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1490..1495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /note="hexamer: ATTAAA"
     polyA_site      1511
                     /gene="Saxo4"
                     /gene_synonym="4930579J09Rik; IIIG9; IIIG9L; IIIG9S;
                     Ppp1r32"
                     /note="major polyA site"
ORIGIN      
        1 gtttcttcta gcttggccat ctccacctca ggagggccag cttctcttga gcagctccag
       61 cctcttgtgg cctctgccga cctcagagct tgctgcagag ggcccgtggg gcccctgcct
      121 cacacatcat gatggggaaa ctccctcttg gggttgtctc tccatatgtg aagatgagtt
      181 cggggggatg ctctgaccct ctgaagtttt atgctaccag ctactgtaca gcctatggtc
      241 gggaggagtt taagccccgc ctgggaagcc atgtaggcac aggctacaaa tccaactatc
      301 ggcccctggt ctcctaccaa ccgcacctgg acaccctgga caatcctgcc atagggcaac
      361 aaatccgaga cacttccaag tctgtgacca gtcagagcta ctctccccta gaagtgcccg
      421 atggtaaaca acccctgccc tggaacctgc accagacaac ctccagctac gggcgagaaa
      481 aactcaaccc tggaccacat tccaaggagg tcaggaaggt ccacttcgac acccaggatc
      541 acgggcctca gactatcaca ggactggagc ccaaggaggt tcccctgatc caccagcagc
      601 agggcaaggg ctccacggag tgggagaact cgcactatgg tccacgcttc atgacttctg
      661 aatacaattc caagtatatc aaggagtctc caaaccaccc agatctcttg cttaagaaga
      721 caattggttc caaagaagag actggcttca cggaagagtc taccaagaat cccattgtct
      781 tccagccgcc ctcacaggcc tttcctgggg accctgtcct ccatccagga cggagtatca
      841 ccaagtcaga ctacctccct gtaactcacc ctcagggaag tgatttcctc cctgtgctgt
      901 ccagaggctc tgatcgggac actggattca gccgagtgaa tgaaaggacc ttgaacccca
      961 gagtgcctac tccagcccca cagtctgcca gcatgagcca caggtcctac cagcctcccc
     1021 agcggatgca acagacaaat gttgccctgc ttggcaggga gtctgtgggg aacaaggagc
     1081 ccacagggtt cactcttaac aaccccagct atgttcggag ttcctatgaa caggacagag
     1141 atcagcggta cctgaccacc tacaaccaag ggtacttcga gaatatcccc aaggggctgg
     1201 atcgagaagg ctggactcga ggtggcatcc agccccagaa agcaggagcc tacgccctca
     1261 gtgagctgaa taaccatacc ctcatggact ccaccccaaa tcccacggag accctgaggc
     1321 acctgcaccc ccatgtggga agaaccctgg cttcagttga ccccttctat cgagacatgc
     1381 ctcatagcag ccgctaccca gcttccagtt gagtctgcag ggtttggcat gacgggacaa
     1441 gctcctcccc ctccgctccc caagattgcc tcggtaactt tactaaggaa ttaaagaaca
     1501 ctgaaccagc a
//
