LOCUS       NM_028773               2692 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus SAM and SH3 domain containing 3 (Sash3), transcript
            variant 2, mRNA.
ACCESSION   NM_028773
VERSION     NM_028773.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Gruber,L.C., Schneider,B., Nothnagel,C. and Beer-Hammer,S.
  TITLE     Knockout of SLy1 decreases double-negative thymocyte proliferation
            and protects mice from p53-induced tumor formation
  JOURNAL   Eur J Immunol 53 (1), e2250017 (2023)
   PUBMED   36401605
  REMARK    GeneRIF: Knockout of SLy1 decreases double-negative thymocyte
            proliferation and protects mice from p53-induced tumor formation.
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Delmonte,O.M., Bergerson,J.R.E., Kawai,T., Kuehn,H.S.,
            McDermott,D.H., Cortese,I., Zimmermann,M.T., Dobbs,A.K.,
            Bosticardo,M., Fink,D., Majumdar,S., Palterer,B., Pala,F.,
            Dsouza,N.R., Pouzolles,M., Taylor,N., Calvo,K.R., Daley,S.R.,
            Velez,D., Agharahimi,A., Myint-Hpu,K., Dropulic,L.K., Lyons,J.J.,
            Holland,S.M., Freeman,A.F., Ghosh,R., Similuk,M.B., Niemela,J.E.,
            Stoddard,J., Kuhns,D.B., Urrutia,R., Rosenzweig,S.D.,
            Walkiewicz,M.A., Murphy,P.M. and Notarangelo,L.D.
  TITLE     SASH3 variants cause a novel form of X-linked combined
            immunodeficiency with immune dysregulation
  JOURNAL   Blood 138 (12), 1019-1033 (2021)
   PUBMED   33876203
  REMARK    GeneRIF: SASH3 variants cause a novel form of X-linked combined
            immunodeficiency with immune dysregulation.
REFERENCE   3  (bases 1 to 2692)
  AUTHORS   Riel,J.M., Yamauchi,Y., Ruthig,V.A., Malinta,Q.U., Blanco,M.,
            Moretti,C., Cocquet,J. and Ward,M.A.
  TITLE     Rescue of Sly Expression Is Not Sufficient to Rescue Spermiogenic
            Phenotype of Mice with Deletions of Y Chromosome Long Arm
  JOURNAL   Genes (Basel) 10 (2), 133 (2019)
   PUBMED   30759861
  REMARK    GeneRIF: We also carried out transgene rescue by adding Sly1/2
            transgenes to mice with NPYq deletions. We demonstrated that SLY1/2
            expression in mutant mice decreased proportionally to deletion
            size, with ~12% of SLY1/2 retained in shSLY sh367 testes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2692)
  AUTHORS   Kukuk,L., Dingley,A.J., Granzin,J., Nagel-Steger,L.,
            Thiagarajan-Rosenkranz,P., Ciupka,D., Hanel,K.,
            Batra-Safferling,R., Pacheco,V., Stoldt,M., Pfeffer,K.,
            Beer-Hammer,S., Willbold,D. and Koenig,B.W.
  TITLE     Structure of the SLy1 SAM homodimer reveals a new interface for SAM
            domain self-association
  JOURNAL   Sci Rep 9 (1), 54 (2019)
   PUBMED   30631134
  REMARK    GeneRIF: In this report, the structure of the SLy1 SAM domain was
            solved and revealed that this SAM domain forms a symmetric
            homodimer through a novel interface. The interface consists
            primarily of the two long C-terminal helices, alpha5 and alpha5',
            of the domains packing against each other.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2692)
  AUTHORS   Schall,D., Schmitt,F., Reis,B., Brandt,S. and Beer-Hammer,S.
  TITLE     SLy1 regulates T-cell proliferation during Listeria monocytogenes
            infection in a Foxo1-dependent manner
  JOURNAL   Eur J Immunol 45 (11), 3087-3097 (2015)
   PUBMED   26306874
  REMARK    GeneRIF: this study describes SLy1 as an immunoregulatory protein,
            which is involved in the generation of adaptive immune responses
            during L. monocytogenes infection, and provides a model of how SLy1
            regulates T-cell proliferation.
REFERENCE   6  (bases 1 to 2692)
  AUTHORS   Reis,B., Pfeffer,K. and Beer-Hammer,S.
  TITLE     The orphan adapter protein SLY1 as a novel anti-apoptotic protein
            required for thymocyte development
  JOURNAL   BMC Immunol 10, 38 (2009)
   PUBMED   19604361
  REMARK    GeneRIF: anti-apoptotic protein required for T cell differentiation
            to the CD4+CD8+ stage, likely to be involved in mTOR complex
            activation
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2692)
  AUTHORS   Scheikl,T., Reis,B., Pfeffer,K., Holzmann,B. and Beer,S.
  TITLE     Reduced notch activity is associated with an impaired marginal zone
            B cell development and function in Sly1 mutant mice
  JOURNAL   Mol Immunol 46 (5), 969-977 (2009)
   PUBMED   18950867
  REMARK    GeneRIF: expression of the Notch signaling mediator RBP-J and the
            Notch target genes Hes-1 and Hes-5 was markedly reduced in MZ but
            not FO B cells of Sly1(d/d) mice.
REFERENCE   8  (bases 1 to 2692)
  AUTHORS   Beer,S., Scheikl,T., Reis,B., Huser,N., Pfeffer,K. and Holzmann,B.
  TITLE     Impaired immune responses and prolonged allograft survival in Sly1
            mutant mice
  JOURNAL   Mol Cell Biol 25 (21), 9646-9660 (2005)
   PUBMED   16227612
  REMARK    GeneRIF: Sly1 is an essential molecular component for the full
            activation of adaptive immunity
REFERENCE   9  (bases 1 to 2692)
  AUTHORS   Beer,S., Simins,A.B., Schuster,A. and Holzmann,B.
  TITLE     Molecular cloning and characterization of a novel SH3 protein (SLY)
            preferentially expressed in lymphoid cells
  JOURNAL   Biochim Biophys Acta 1520 (1), 89-93 (2001)
   PUBMED   11470164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY204396.1, BY209546.1, AK004734.2 and BC028773.1.
            
            On Sep 1, 2015 this sequence version replaced NM_028773.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            exon in the 3' end compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064734.1, AK156202.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                BY204396.1         2-46
            46-91               BY209546.1         7-52
            92-2570             AK004734.2         2-2480
            2571-2692           BC028773.1         2042-2163
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 25.54 cM"
     gene            1..2692
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /note="SAM and SH3 domain containing 3"
                     /db_xref="GeneID:74131"
                     /db_xref="MGI:MGI:1921381"
     exon            1..282
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..1368
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /note="isoform 2 is encoded by transcript variant 2; SAM
                     and SH3 domain-containing protein 3; Novel SH3 and SAM
                     domain containing protein; SH3 protein expressed in
                     lymphocytes"
                     /codon_start=1
                     /product="SAM and SH3 domain-containing protein 3 isoform
                     2"
                     /protein_id="NP_083049.1"
                     /db_xref="CCDS:CCDS40959.1"
                     /db_xref="GeneID:74131"
                     /db_xref="MGI:MGI:1921381"
                     /translation="MLRRKPSNASDKEPTQKKKLSLQRSSSFKDFAKSKPSSPVVSEK
                     EFNLDDNIPEDDSGVLTPEDSGKSGKKLGKKWRAVISRTMNRKMGKMMVKALSEEMGD
                     TLEEGSASPTSPDCSLDSPGPEKMALAFTEQEEREPPSLSRQTSTGSELCSPGPGSGS
                     FLEESPAPQYTGPFCGRARVHTDFTPSPYDHDSLKLQKGDVIQIVEKPPVGTWLGLLN
                     GKLGSFKFIYVDVLPEEAVGPVRPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLN
                     GYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPV
                     AHTVSEPKVDIPRDSGCFEGSESGRDEAELAGTEEQLQGLSLSGAP"
     exon            283..378
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            379..525
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..667
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            668..816
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1026
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1177
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..2677
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2662..2667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /note="hexamer: TATAAA"
     polyA_site      2677
                     /gene="Sash3"
                     /gene_synonym="1200013B08Rik; SLY1"
                     /note="major polyA site"
ORIGIN      
        1 atgattgctg taagagcagg tcacgtggca aggtttcctg tatgctgctg ctgctgggtg
       61 tccatggccc gcacccccaa gctgccactg cagcagtaaa gagtagcagc cccaggctca
      121 gtctatgctg tgccaactgg ctggtgctgg ggaggcttta acaagagatt tttgcatctt
      181 agctccttgg agaacagagc aggagtgcca tttctgtaga ggaccatgtt gcgtcgcaaa
      241 ccctccaacg ccagtgacaa agagcccact cagaagaaaa agctctcact tcagcgctcc
      301 agcagcttca aggattttgc caaatcgaag cccagctccc ccgtggtgag cgagaaagaa
      361 tttaacctgg atgataatat tccagaagat gattcaggtg ttcttacccc ggaggattct
      421 gggaagagtg gcaaaaagtt gggaaagaag tggagggcag tcatttcccg aaccatgaac
      481 aggaagatgg gcaagatgat ggtgaaggcc ttgtcagagg agatgggaga cacgctggaa
      541 gagggctcag cttctccaac atctccagac tgcagcctgg acagccccgg gcctgagaag
      601 atggccctgg cctttactga gcaggaggag cgtgagccgc catctctcag ccgccagaca
      661 tcaacaggca gtgaactctg tagccctggc ccaggctcgg gaagcttcct agaggagtca
      721 ccggctcccc agtacacggg gcctttctgt gggcgtgccc gagtccacac tgacttcact
      781 cccagccctt atgaccacga ctcattgaaa ctgcagaaag gagatgtgat ccagatcgtt
      841 gaaaagccac ctgtgggcac atggctgggc ctgctcaatg gcaagctggg ttctttcaaa
      901 ttcatctacg tggatgtgct acccgaggag gctgtgggac ctgtacgccc cagtcgccga
      961 cagagcaaag gcaagaggcc caagcccaag actctgcatg aactgctgga gcgcattggc
     1021 ctagaggaac acacatccac cctactgctc aatggctacc agacgctcga agacttcaaa
     1081 gagctgcggg aaacgcacct caatgagctg aacatcatgg acccacaaca ccgggccaag
     1141 ctgctcacag ctgcagagct gctactggac tatgacactg gcagtgaaga ggctgaagag
     1201 ggtgctgaga gcagccagga acccgtggcg cacacagtgt cagaacccaa ggtggacatc
     1261 ccacgtgact ccggctgctt tgagggctca gagagtgggc gtgatgaggc agagctggca
     1321 ggcacagaag agcaactgca gggtctctcc ctgtctgggg caccttgatg cgaaaatgtc
     1381 ctttaaggct gaggctgaac cctagctgca gagactgcag ggatgggtgt ggcctcccat
     1441 aatggcatag gaccagagga ccggtgcaga atctggccct gcttccccag agaggcttat
     1501 ggttgaggcc cctaccagga tgctacaggc tcagatagag tggataaggg actgcccaaa
     1561 ggcatatgct gccctttgga ttctctccct ccaccagtga ctgacaatat ggccccttct
     1621 ggcacctcct attctcccac tatggccatg caagtggggc atcaagggac ctagccatgc
     1681 catgtgatca cggccccaaa aaccttctta ccctcacacc actgtcccat tgcttctaag
     1741 aaggacgcca agccactgtc tcaaagcact ctttaagccc tggggaagct tgctccttca
     1801 aaggatctca ggtggacata agtgagtttg gaattcctta aatgaggttt gaaagaacct
     1861 tcacacccgt tctccactat ccttctcaac attttcgagg tctgaccact aagtcacaag
     1921 acttgaacta tgtctgtgct tcttttgact tcatcctggt cgggattcga ataggcagtt
     1981 attgagggtc ccaccagttg acactgtgta cctttaacta tttgctctcc cgctgcctgc
     2041 tagccaggac accagtgagt aggcttcttg aagaggctgt aagggcctag ggtttccagt
     2101 gaaatagcgc tgccttctca gtcatctcat acctttctgt gtgacccaaa ctgagtgccg
     2161 ctgtttccca acttagaaca atgcccatag ctcatatgat aagtgtctcc acctgttcct
     2221 tggctccttc aaatgagatt tcctacaggc tggttgaaga tggtgacacc tggcttccaa
     2281 tgatgcacct gcctcctgca agccagatgc tggtatggac ccaggcttgg tcatttatgt
     2341 ggatcttctg aagcaaaggg agcctttgat tgacattgga ggctccacta cacccaacat
     2401 tgctctgcta aaggttttag tgtcctaact ccacaggatc aggagattat cacaactatt
     2461 actggggtac ccttgactcc aatagcaagg gcagtccttc ctgaagtggg gtcaactgtg
     2521 cccatcacag gtaggactac ctgcttagta agtgggcatg cttctgatcc actactacca
     2581 gggactttca taacttcgag tttgggcaaa ggagttgaat gtacataagg agtagcgggg
     2641 agccaaagta gtagcaataa atataaatag aacttaaaaa aaaaaaaaaa aa
//
