LOCUS       NM_001204979            3632 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus seryl-tRNA synthetase 1 (Sars1), transcript variant 2,
            mRNA.
ACCESSION   NM_001204979
VERSION     NM_001204979.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Zhao,J., Bai,H., Li,X., Yan,J., Zou,G., Wang,L., Li,X., Liu,Z.,
            Xiang,R., Yang,X.L. and Shi,Y.
  TITLE     Glucose-sensitive acetylation of Seryl tRNA synthetase regulates
            lipid synthesis in breast cancer
  JOURNAL   Signal Transduct Target Ther 6 (1), 303 (2021)
   PUBMED   34400610
  REMARK    GeneRIF: Glucose-sensitive acetylation of Seryl tRNA synthetase
            regulates lipid synthesis in breast cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3632)
  AUTHORS   Wei,N., Shi,Y., Truong,L.N., Fisch,K.M., Xu,T., Gardiner,E., Fu,G.,
            Hsu,Y.O., Kishi,S., Su,A.I., Wu,X. and Yang,X.L.
  TITLE     Oxidative stress diverts tRNA synthetase to nucleus for protection
            against DNA damage
  JOURNAL   Mol Cell 56 (2), 323-332 (2014)
   PUBMED   25284223
REFERENCE   4  (bases 1 to 3632)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3632)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   6  (bases 1 to 3632)
  AUTHORS   Martinis,S.A., Plateau,P., Cavarelli,J. and Florentz,C.
  TITLE     Aminoacyl-tRNA synthetases: a family of expanding functions.
            Mittelwihr, France, October 10-15, 1999
  JOURNAL   EMBO J 18 (17), 4591-4596 (1999)
   PUBMED   10469639
REFERENCE   7  (bases 1 to 3632)
  AUTHORS   Miseta,A., Woodley,C.L., Greenberg,J.R. and Slobin,L.I.
  TITLE     Mammalian seryl-tRNA synthetase associates with mRNA in vivo and
            has homology to elongation factor 1 alpha
  JOURNAL   J Biol Chem 266 (29), 19158-19161 (1991)
   PUBMED   1918033
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY034232.1, AK075827.1, AK028358.1 and BP765728.1.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            3' coding region, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028358.1, SRR1660819.136379.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                BY034232.1         1-70
            71-1914             AK075827.1         2-1845
            1915-3631           AK028358.1         1847-3563
            3632-3632           BP765728.1         34-34               c
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 47.08 cM"
     gene            1..3632
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="seryl-tRNA synthetase 1"
                     /db_xref="GeneID:20226"
                     /db_xref="MGI:MGI:102809"
     exon            1..263
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1666
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /EC_number="6.1.1.11"
                     /note="isoform 2 is encoded by transcript variant 2;
                     seryl-aminoacyl-tRNA synthetase 1; seryl-tRNA synthetase,
                     cytoplasmic; seryl-tRNA(Ser/Sec) synthetase; serine--tRNA
                     ligase, cytoplasmic"
                     /codon_start=1
                     /product="serine--tRNA ligase, cytoplasmic isoform 2"
                     /protein_id="NP_001191908.1"
                     /db_xref="CCDS:CCDS57254.1"
                     /db_xref="GeneID:20226"
                     /db_xref="MGI:MGI:102809"
                     /translation="MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWR
                     RCRFRADNLNKLKNLCSKTIGEKMKKKEAVGDDESVPENVLNFDDLTADALAALKVSQ
                     IKKVRLLIDEAIQKCDGERVKLEAERFENLREIGNLLHPSVPISNDEDADNKVERIWG
                     DCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGPLVFLEQALIQYALRTLGSR
                     GYTPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPI
                     AALHRDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHD
                     NKSWEMFDEMIATAEEFYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFREL
                     VSCSNCTDYQARRLRIRYGQTKKMMDKVEFVHMLNATMCATTRTICAILENYQAEKGI
                     AVPEKLREFMPPGLQELIPFVKPAPIDQEPSKKQKKQHEGSKKKAKEVPLENQLQSME
                     VTEA"
     exon            264..334
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            335..415
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            416..574
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            575..718
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            719..874
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1096
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1226
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1384
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1514
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..3632
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1862..1867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="hexamer: AATAAA"
     polyA_site      1915
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="major polyA site"
     polyA_site      1923
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="major polyA site"
     regulatory      1988..1993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="hexamer: ATTACA"
     polyA_site      2019
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="hexamer: AGTAAA"
     polyA_site      2067
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
     polyA_site      2088
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
     polyA_site      2556
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
     regulatory      3369..3374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
                     /note="hexamer: AACAAA"
     polyA_site      3405
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
     polyA_site      3632
                     /gene="Sars1"
                     /gene_synonym="Sars; serRS; Strs"
ORIGIN      
        1 gctaggcgcc gccccctccc gggctgccgg gtgcctgcgc agtgagacca ggtcagcgcg
       61 caggcgcagt gcatcgtcca cccgccgctt acagtcctct cttagtgtta actggtgagg
      121 tagaaggatg gtgctggacc tggatttgtt tcgggtggat aaaggagggg acccagccct
      181 cattcgagag acgcaggaga agcgcttcaa ggacccgggg ctggtggacc agctggtgaa
      241 agcagacagt gagtggcgac gatgcagatt tcgggcagac aacttgaaca agctgaagaa
      301 tttatgcagc aaaactattg gggagaaaat gaagaaaaag gaagcagtgg gagacgacga
      361 gtccgtccca gagaacgtgc tgaatttcga tgacctcact gcagacgcgc tagctgccct
      421 gaaagtctca cagattaaaa aagtccgact cctcattgat gaagccatcc agaagtgtga
      481 tggggagcgg gtaaagctgg aagcagagcg atttgagaac ctccgcgaga ttgggaacct
      541 tctgcacccc tctgtgccca ttagtaatga tgaggacgca gacaacaaag tagaacgtat
      601 ttggggagat tgtacagtca ggaagaagta ttcccatgtg gacctggtgg tgatggtaga
      661 tggctttgaa ggcgaaaagg gagccgtggt ggctggtagt cgggggtact tcctgaaggg
      721 gcccctggtg ttcctggagc aggcgcttat ccaatatgca ctgcgtacct tggggagtcg
      781 gggctacact ccaatctaca cccccttctt catgaggaaa gaggtcatgc aggaagtggc
      841 ccagctcagc cagtttgatg aagaacttta taaggtgatt ggcaaaggca gcgaaaagtc
      901 agatgacaac tcctatgacg agaaatactt gattgccacc tcagagcagc ccatcgcggc
      961 tctgcaccgg gacgagtggc tgcggccaga ggatctgccc atcaagtacg ctggcctctc
     1021 cacctgcttt cgtcaggaag tgggctcgca tggccgtgac acccgtggta tcttccgagt
     1081 ccatcagttt gagaagattg agcagtttgt gtactcatcg ccccatgaca ataagtcgtg
     1141 ggagatgttt gatgagatga tcgccaccgc agaagaattc taccagtctt tggggatccc
     1201 ttaccacatt gtgaatattg tctcaggctc cttgaatcac gctgccagta agaagctcga
     1261 cctggaggcc tggttcccag gctcgggtgc cttccgtgag ttggtgtcct gttctaattg
     1321 cacggattac caagctcgcc gcctgagaat ccgatatggg cagaccaaga agatgatgga
     1381 caaggtggag tttgtccata tgcttaatgc tacaatgtgt gctaccaccc ggaccatctg
     1441 cgccatcctg gagaactacc aggcagagaa gggcatcgct gtgccagaga agttgaggga
     1501 gttcatgccg ccagggctcc aagagctgat cccgtttgtg aagcctgcac ccattgacca
     1561 ggagccatct aagaagcaga agaagcaaca tgaaggcagc aaaaagaaag cgaaagaggt
     1621 ccccctggag aaccagctgc agagcatgga ggtcactgag gcctgagcac tgccagactt
     1681 acttactaag cctgctggga tcccaggcct gccctgggac agagaggccg agaaccactc
     1741 ctcaccctgt cccatctgac tgcacagcgg ctggggaaac catctgccgt ggacagagca
     1801 ggtgttccct tctcctcgca cgggccaggg gtctcttcac tgatgactgg tgaagccacg
     1861 caataaagca tctctgggga gggcacaagg cttccccagt ctcctcccct ccccagactt
     1921 gaaccttgtc tctgctgggt ctccctgggc ctgaactgct ctcccagata aggcttcttt
     1981 gagaatgatt acagagccct cagccagggt gttagtaaag aggtatattt atttagtaaa
     2041 ggctttttag aaagcctgga gttgtaacac aagttgtcag gacaaggatt gggattctct
     2101 gtaaagttac tcctgcctga aggctgtgac gcgcttagag accagtatga tggaaaagcc
     2161 tgctagcacg cacatacatt ccctaggaag ggcttctcgc agagagaagg ctgagtgtgg
     2221 tagctgacac tcctccacag gagcccctag gttccaaagc acgcgctgtg gttatgaaca
     2281 gcagcttccg tctttacagc tgtccccgcg tggggagagg ctcgccacat agcagtcact
     2341 tgctgtagtg tcagaggcag ggcagagggg tgcaggacgg gtctgtcctc aggaactccc
     2401 actgtgtcct aatagctggg cacctgcccc cccgggaagg cagtagtcag gctttcttca
     2461 ccagctctct ttcatctgtg atctgtatcc tccgctctaa tgacacctta ggtcagtctg
     2521 gagcttgcct tctagcaggt taaagcgctt cctctagatg ctgcctttta tcttcgtgtg
     2581 gccccagaag gtagggtggg ggcatgatta tacctgtccc agagtgggag aatgaggtag
     2641 cgtggccagg tgggagtctg gctcagagtg acttgcagag gttgggagct gagtatgacc
     2701 ctgcttgtga cagggcttca gctcagccca cctcaactgc tttggcccag taccaagcaa
     2761 gcaaagaggc cgtgtgctta tttcttctag ggagttggtt aaccttgctc ttggtaagcc
     2821 tgtcaaaact gggtgcctac aagggggaaa caccgaggct cttctgcctg ctgtggcagc
     2881 tggctgccca ggcaggctcc gtggcccctt ggcttcagcc ctgggaaaca gcctggacaa
     2941 gaggacaaag agccaggctg ctcccatttg tgattaattc acattcccat aaccttaaca
     3001 ttcagttaga agaaaagcag cttgaaccgt gatggggaga ctaggctgtc ccgcttacaa
     3061 gtttgttagg aatagctagg tggtcctgtg ttgtcttggg cctgacctag gtctccacag
     3121 ctgctccccc cacccccgtc ctctaacgcc tgtagccttg ggcacagaca gggttctgcc
     3181 ttcctgtatc cgctcttgtc tccccttcac tagccagctt tactctctac cttgccagtt
     3241 tcctggagca gcctgctctt ggactctaga caccctgagc agtcagtcat ttctccccac
     3301 atctgtcttc cccagagagg gccaagtaat cttcacagaa tgtgtgcctc attgccaaac
     3361 cagaggagaa caaaaaaatg ggagtcttcc catgcttcat acaaatagaa aacagaccac
     3421 tgtcccatgc tctcaggaca agagctaact caggttctca acctgtgagt cgtgacctcc
     3481 ttgacaaacc cttttcccaa aatatttaca ttatgcttta tgtaattagc aaaattgcag
     3541 ttatgaggta acagcatgaa ttttatggtt gggggccacc acaacacgtg gaactgtatt
     3601 taagggttgc agcattaaga aggttaaccg ct
//
