LOCUS       NM_138665               4573 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sarcosine dehydrogenase (Sardh), mRNA; nuclear gene
            for mitochondrial product.
ACCESSION   NM_138665
VERSION     NM_138665.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4573)
  AUTHORS   Ai,L., Tan,T., Tang,Y., Yang,J., Cui,D., Wang,R., Wang,A., Fei,X.,
            Di,Y., Wang,X., Yu,Y., Zhao,S., Wang,W., Bai,S., Yang,X., He,R.,
            Lin,W., Han,H., Cai,X. and Tong,Z.
  TITLE     Endogenous formaldehyde is a memory-related molecule in mice and
            humans
  JOURNAL   Commun Biol 2, 446 (2019)
   PUBMED   31815201
  REMARK    Erratum:[Commun Biol. 2019 Dec 19;2:479. doi:
            10.1038/s42003-019-0738-2.. PMID: 31872079]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4573)
  AUTHORS   Kiyama,T., Chen,C.K., Wang,S.W., Pan,P., Ju,Z., Wang,J., Takada,S.,
            Klein,W.H. and Mao,C.A.
  TITLE     Essential roles of mitochondrial biogenesis regulator Nrf1 in
            retinal development and homeostasis
  JOURNAL   Mol Neurodegener 13 (1), 56 (2018)
   PUBMED   30333037
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4573)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   6  (bases 1 to 4573)
  AUTHORS   Harding,C.O., Williams,P., Pflanzer,D.M., Colwell,R.E., Lyne,P.W.
            and Wolff,J.A.
  TITLE     sar: a genetic mouse model for human sarcosinemia generated by
            ethylnitrosourea mutagenesis
  JOURNAL   Proc Natl Acad Sci U S A 89 (7), 2644-2648 (1992)
   PUBMED   1372986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731552.11, AK163515.1 and AW495221.1.
            
            On Mar 18, 2011 this sequence version replaced NM_138665.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159704.1, AK159681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-623               AL731552.11        24651-25273         c
            624-4556            AK163515.1         153-4085
            4557-4573           AW495221.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.31 cM"
     gene            1..4573
                     /gene="Sardh"
                     /note="sarcosine dehydrogenase"
                     /db_xref="GeneID:192166"
                     /db_xref="MGI:MGI:2183102"
     exon            1..651
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            652..1006
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     CDS             673..3432
                     /gene="Sardh"
                     /EC_number="1.5.8.3"
                     /codon_start=1
                     /product="sarcosine dehydrogenase, mitochondrial"
                     /protein_id="NP_619606.1"
                     /db_xref="CCDS:CCDS15826.1"
                     /db_xref="GeneID:192166"
                     /db_xref="MGI:MGI:2183102"
                     /translation="MASLSRVLRVAATCPRGRAAWNLGLQPLATEARPTTEKSVPYQR
                     TLKEEAQGASVVPQGPSQPLPSTANVVVIGGGSLGCQTLYHLAKLGVGGAVLLERERL
                     TSGTTWHTAGLLWQLRPSDVEVELLAHTRQVVSRDLEEETGLHTGWIQNGGLFIASNQ
                     QRLNEYKRLMSLGKAYGIESHVLSPAETKSLYPLMNVDDLYGTLYVPQDGTMDPAGTC
                     TTLTRAAVARGAQVIENCAVTGIRVRTDDFGVRRVAAVETEHGSIQTPCVVNCAGVWA
                     SKVGRMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEA
                     NPIFWEEVSDKFAFGLFDLDWDVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHK
                     PLMGEAPELRGFFLGCGFNSAGMMLGGGCGQELAHWIVHGRPEKDMYSYDIRRFHHSL
                     TDHTRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLHEELLGQGCVFQERQGWER
                     PGWFNPQETAQVLDYDYYGAYGNQAHKDYTYSRLLGDEYTFDFPPHHHMIQKECLACR
                     GAAAVFNMSYFGKFYLLGVDARKAADWLFSADVNRPPGSTVYTCMLNQRGGTESDLTV
                     SRLAPGTQASPLVPAFEGDCYYLAVGGAVAQHNWSHINTVLQDQEFRCQLMDSSEDLG
                     MLSIQGPASRDILQDVLDADLSNEAFPFSTHQLVRAAGHLVRAIRLSFVGELGWELHV
                     PRASCLPVYRAVMAAGARHGLVNAGYRAIDSLSIEKGYRHWHADLRPDDSPLEAGLAF
                     TCKLKTSVPFLGREALEKQRATGLRRRLICLTVEEEVPMFGLEAIWRNGQVVGHVRRA
                     DFGFTVNKTIAYGYIRDPSGGPVSLDFVKNGEYALERMGVTYAAQVHLKSPFDPDNKR
                     VKGIY"
     exon            1007..1185
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1365
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1489
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1590
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1695
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1825
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1912
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2003
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2145
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2229
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2230..2343
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2482
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2483..2596
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2744
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2745..2838
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..3001
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3170
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3306
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     exon            3307..4558
                     /gene="Sardh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3543..3548
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sardh"
                     /note="hexamer: AATAAA"
     polyA_site      3569
                     /gene="Sardh"
                     /note="major polyA site"
     regulatory      4533..4538
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sardh"
                     /note="hexamer: AATAAA"
     polyA_site      4558
                     /gene="Sardh"
ORIGIN      
        1 agggaggggc agtgtccccg acagcccctt catgggcagg gttggagcta gctgcttgct
       61 agctgggacc tccctcttct tctggttgaa agaccgtgga gtagatggga cggccatgga
      121 taactgtggg agggcaggag ggggggtccc ttatgagagg gctctgcaga gtgtcttccc
      181 ctcttgggct ctttctcata cccttctagt gctgccttaa aaaagggccc tgtgtctgag
      241 gcctcaggtc tgtgactgag ttctcattcc ctgtgtcctg tgccctggcc cttggaaagc
      301 tgagggagag agctgcagag gccagagggc aggttaatga agtacctgtt ctgaaggagg
      361 aggagcaggc tgggggcctg gctgccccac cccccacccc tgctctgact agctgggctg
      421 gcaaggaagg agcacgtggc agctccttgg gccagcagca ttggcactgg gcatagtttg
      481 cagaggaagg aggctcctct ggcatgtatg cagtgcctgg catggtgtcc taaggtagag
      541 gcactgtttg gacttgcaga gccccgattg gatccatatc atgctgactt ttccttgcta
      601 ggaacctctc aggagcttct ggaagaccaa ggaacccatt gaaagactaa ggtacaggag
      661 ccctaagtcc ccatggcttc actgagccgg gtcctacgtg tagctgccac ctgtcctcga
      721 gggagagctg cttggaatct ggggctacaa cccctagcca cagaggccag gcccaccacc
      781 gagaagagtg ttccttatca gcggaccctg aaggaggagg cccaaggtgc ctccgtggtg
      841 ccccaaggac caagccagcc cctgcctagc acagccaatg ttgtggtcat cggtgggggc
      901 agtttgggct gccagactct gtatcacttg gctaagctgg gtgtaggtgg agccgtgctg
      961 ctggagagag agcgactgac ctctgggacc acctggcaca cagcaggctt actgtggcag
     1021 ctgcggccca gcgatgtgga ggtagagctt ttggctcaca cacggcaagt ggtgagccgt
     1081 gatctggaag aggagactgg gctgcataca ggctggatcc agaatggtgg cctcttcatc
     1141 gcctctaacc agcaacgcct gaatgaatac aagaggctca tgtcgttggg caaggcctat
     1201 ggtatagaat cccacgtgct gagcccagca gagaccaaga gtctatatcc gctgatgaac
     1261 gtggacgacc tctatgggac cctgtatgtg ccacaggatg gtaccatgga ccccgctggc
     1321 acctgtacta ccctcaccag agcagcagtg gctcgaggag ctcaggtcat tgaaaactgt
     1381 gcggtgactg gcatccgtgt gcggacggac gactttggag tgcggcgggt ggcagctgtg
     1441 gagacagagc atggctccat ccagacaccc tgtgtggtca actgtgcagg agtgtgggcg
     1501 agcaaggtgg gccgcatggc tggagtgaag gtcccgctgg tggccatgca ccatgcttac
     1561 gtcgtcactg agcgcatcga ggggatccag aacatgccca atgtccggga tcacgatgct
     1621 tctgtctacc tccgactgca gggggatgcc ttgtctgtgg gtggctatga ggccaaccct
     1681 atcttttggg aggaggtgtc agacaaattt gcctttggcc tctttgacct ggactgggat
     1741 gtgttcaccc agcacattga aggcgccatt aaccgtgtcc ctgtgttgga gaagacgggg
     1801 atcaagtcca ctgtctgtgg tcctgaatcc ttcacacctg accataagcc cctgatgggg
     1861 gaggctcctg aactccgtgg cttcttcctt ggctgtggct tcaacagtgc aggcatgatg
     1921 ttgggtggtg gctgtggaca agagctggcc cattggattg tgcacgggcg cccagagaag
     1981 gacatgtaca gctatgacat caggcgtttc catcactcgc tcacggacca cacccgctgg
     2041 atccgagagc gcagccatga gtcctatgcc aagaactact cggttgtctt cccacacgat
     2101 gagccactgg caggacgcaa catgaggaga gaccccctgc atgaggagct gcttggacaa
     2161 ggctgtgtgt ttcaagagcg acagggatgg gaacggccgg gatggttcaa cccccaggaa
     2221 acagctcagg tccttgacta cgactactac ggggcctacg gaaaccaggc acacaaggac
     2281 tacacctaca gcaggttgct aggcgatgag tacaccttcg acttcccacc ccaccaccac
     2341 atgatccaga aggaatgtct ggcctgcaga ggggctgctg ctgtgttcaa catgtcttac
     2401 tttgggaagt tctacctgct cggggtggac gccaggaagg ctgcagactg gctcttctca
     2461 gcagatgtca accgaccccc aggctcaaca gtgtacacat gcatgctgaa ccaacgggga
     2521 ggcactgaga gcgacctgac tgttagccga ttggctcctg gcacccaggc cagtcccctg
     2581 gtccctgcct ttgaagggga ttgctactac ctggccgtgg gtggggctgt ggcccaacac
     2641 aactggtccc acataaacac ggtgctacag gaccaggagt tccggtgcca gcttatggat
     2701 agttctgaag atctgggcat gctcagtatc cagggcccag ccagccgtga catcctgcag
     2761 gatgtgcttg atgctgacct gagcaacgag gccttcccct tctccaccca ccagctggtg
     2821 agagccgccg ggcacctggt ccgggccatc aggctgtcct ttgtcgggga gctgggatgg
     2881 gagctgcatg taccccgggc atcctgcttg ccagtgtacc gggctgtgat ggctgcaggt
     2941 gcgaggcatg gcctagtcaa cgcaggctac cgtgccatcg actccctgag catcgagaaa
     3001 ggctatagac actggcatgc agacctgagg cccgatgaca gccccctgga ggcaggattg
     3061 gccttcacct gcaaactcaa gacctcggtg cccttcctag gccgagaggc cctggagaag
     3121 cagcgtgcca caggtctccg ccgacgcctg atatgcctca ctgtggaaga ggaggtgcct
     3181 atgtttggcc tggaggccat ctggagaaac ggccaagtgg tgggccatgt ccggagagct
     3241 gactttggat ttactgtgaa caagaccatc gcctatggct acatccgaga ccccagtggt
     3301 ggccctgtct ctctggactt tgtgaagaat ggagagtatg ccttggagag aatgggggtc
     3361 acctatgctg cccaggttca cctgaagtct ccctttgacc ctgacaacaa gcgggtgaag
     3421 ggaatctatt gatgggatgc aggtggaggc tacagctgct caggccactg gcaacagcca
     3481 tgctccccac actgcctgcg gacccctgca ctgcacacac agccttgtgt cctgagaccc
     3541 agaataaagg caaagcacag acttccccac ccttagcctc catgtggtct tgctaaccac
     3601 cacagttcct aaccagggct gggtggagca ctatgttcct cctgctcatg agccacacag
     3661 tggtctccag tccgctctag ccctcccaaa attcaccctg cagatgtctt ggctcaaggt
     3721 ccctgtgagt atcttaggtg tatggctaac ccaagatgga agctgtaaag caggcttccc
     3781 ctctgtccct cactcagctg actgccagag tagtggatcg ggagagtgaa tggcgattga
     3841 tcagcaatgc ctgccacagg gtggggagca catagatggg tgctagactg gtacaatcct
     3901 tcctcagaat ggctgccttt cagaccagag aggagaaggg taagaaagct cttacagcct
     3961 tccagctctc caggagataa agccttctaa gagtcaccat gggatttgga tcaagaactt
     4021 ccattactta caaagaagtg acacattctg cctcagtttc ccctcaagct tttgctcatg
     4081 ggtccgggga caccaagctt gctcttgtgc atgtttccaa ggactttctg tctctgtcac
     4141 agatgcatgg ctccagctct gctctgtgag atagagcggt cccttctccc atcctgtgta
     4201 accatctaca tatgtcctca tgtcaagggc accctggctc tccttcctgg cggtgaatgt
     4261 ctatcacttt tctatggccc ctgtcttctt cctctgcctc acgccctgac tgagaaatta
     4321 ggatgccatg tctccccaaa cgaaggtcac catgtagtct tacatgcctt gtggtctagg
     4381 agggcaggag gcagatgcca tagtggtacg gagctggggt gtcctaaacc taaagaaggc
     4441 agcagagggg atatgtgggc ccagctgtcc tgggtgggct ctgaatcttc ataggtttac
     4501 acaccacaca ctgagctgga aaaggtcaag ttaataaaat cgtgtgaaac cactggaaaa
     4561 aaaaaaaaaa aaa
//
