LOCUS       NM_026283               7015 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus sterile alpha motif domain containing 8 (Samd8),
            transcript variant 1, mRNA.
ACCESSION   NM_026283 XM_979532 XM_979573 XM_979603 XM_979647 XM_979679
            XM_979722
VERSION     NM_026283.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Chiang,Y.P., Li,Z., He,M., Jones,Q., Pan,M., Han,X. and Jiang,X.C.
  TITLE     Sphingomyelin synthase-related protein SMSr is a
            phosphatidylethanolamine phospholipase C that promotes nonalcoholic
            fatty liver disease
  JOURNAL   J Biol Chem 299 (9), 105162 (2023)
   PUBMED   37586586
  REMARK    GeneRIF: Sphingomyelin synthase-related protein SMSr is a
            phosphatidylethanolamine phospholipase C that promotes nonalcoholic
            fatty liver disease.
            Erratum:[J Biol Chem. 2024 Apr;300(4):107204. doi:
            10.1016/j.jbc.2024.107204.. PMID: 38552502]
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Bickert,A., Ginkel,C., Kol,M., vom Dorp,K., Jastrow,H., Degen,J.,
            Jacobs,R.L., Vance,D.E., Winterhager,E., Jiang,X.C., Dormann,P.,
            Somerharju,P., Holthuis,J.C. and Willecke,K.
  TITLE     Functional characterization of enzymes catalyzing ceramide
            phosphoethanolamine biosynthesis in mice
  JOURNAL   J Lipid Res 56 (4), 821-835 (2015)
   PUBMED   25667419
  REMARK    Erratum:[J Lipid Res. 2017 Apr;58(4):820. doi:
            10.1194/jlr.M055269ERR.. PMID: 28365667]
REFERENCE   3  (bases 1 to 7015)
  AUTHORS   Ding,T., Kabir,I., Li,Y., Lou,C., Yazdanyar,A., Xu,J., Dong,J.,
            Zhou,H., Park,T., Boutjdir,M., Li,Z. and Jiang,X.C.
  TITLE     All members in the sphingomyelin synthase gene family have ceramide
            phosphoethanolamine synthase activity
  JOURNAL   J Lipid Res 56 (3), 537-545 (2015)
   PUBMED   25605874
  REMARK    GeneRIF: study reveals that all mouse SMS family members (SMSr,
            SMS1, and SMS2) have CPE synthase activity
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK006211.1, AC151533.3 and BQ176258.1.
            
            On Jan 24, 2018 this sequence version replaced NM_026283.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006211.1, SRR10223971.13962.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1560              AK006211.1         2-1561
            1561-6997           AC151533.3         33129-38565         c
            6998-7015           BQ176258.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 11.86 cM"
     gene            1..7015
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="sterile alpha motif domain containing 8"
                     /db_xref="GeneID:67630"
                     /db_xref="MGI:MGI:1914880"
     exon            1..250
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1513
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /EC_number="2.7.8.48"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sphingomyelin synthase-related protein 1; GrailEXP
                     prediction; CPE synthase; SAM domain-containing protein 8;
                     ceramide phosphoethanolamine synthase; sterile alpha motif
                     domain-containing protein 8"
                     /codon_start=1
                     /product="sphingomyelin synthase-related protein 1 isoform
                     1"
                     /protein_id="NP_080559.1"
                     /db_xref="CCDS:CCDS26866.1"
                     /db_xref="GeneID:67630"
                     /db_xref="MGI:MGI:1914880"
                     /translation="MPAGSRAGSRLRSGSLPRPSRLTLKALRPAYAPRTPDSNGDLDT
                     GSELGPGSPAPTAEEVEKEMAGPSQLCIRRWTTKHVAVWLKDEGFFEYVDILCNKHRL
                     DGITLLTLTEYDLRSPPLEIKVLGDIKRLMLSVRKLQKIHTDVLEEMGYNSDSPMSPM
                     TPFLSALQSADWLCNGEPTHSCDGPIPDLSSDQYQYMNGKNKHSARRLDPEYWKTILS
                     CVYVFIVFGFTSFIMVIVHERVPDMQTYPPLPDIFLDSVPRIPWAFSMTEVCGVILCY
                     IWILVLLLHKHRSILLRRLCSLMGTVFLLRCFTMFVTSLSVPGQHLQCTGKIYGSVWE
                     KLRRAFAIWSGFGMTLTGVHTCGDYMFSGHTVVLTMLNFFVTEYTPRSWNFLHTLSWV
                     LNLFGIFFILAAHEHYSIDVFIAFYITTRLFLYYHTLANTRAYHQSRRARIWFPMFSF
                     FECNVNGTVPNEYCWPFSKPAIMKRLIG"
     exon            251..843
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            844..939
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1057
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1208
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..6998
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1545..1550
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="hexamer: AATATA"
     polyA_site      1574
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="major polyA site"
     regulatory      6976..6981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="hexamer: AATAAA"
     polyA_site      6998
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="major polyA site"
ORIGIN      
        1 gtagcgcccc gcctcccgaa gcagttccgg ttcggctggc cgctgctggc ggcacggctc
       61 caaaggggcc ggagcgatgc ccgctggtag ccgagccggc tcccgcctgc gctccggctc
      121 cctcccccgg ccttcgcgtc tgactctcaa ggcgctgcgc cccgcctacg cgccgaggac
      181 ccccgactcc aacggcgact tagacaccgg ctccgagctg ggtcccggca gcccggctcc
      241 caccgcggag gaagtagaga aagaaatggc aggtcctagt caactctgca ttcgccgctg
      301 gactaccaag catgtggctg tgtggctgaa ggacgaaggt ttctttgagt atgtggacat
      361 tttatgcaac aagcaccgcc ttgatggaat cacattgctc acgctgactg aatatgatct
      421 ccgctctcct cctctggaaa tcaaagtcct aggagacatt aaacggttaa tgctctcagt
      481 ccgaaaattg cagaaaatac atactgatgt tttggaagag atgggctaca acagtgacag
      541 tcctatgagt cccatgactc cattccttag tgctcttcag agtgcagact ggctctgtaa
      601 cggagagccc acacacagct gtgatgggcc catccctgac ttgagttctg atcagtacca
      661 gtacatgaat ggcaaaaaca aacattctgc tcgaagactg gacccagagt actggaagac
      721 catcctgagt tgtgtatatg tttttatagt atttgggttt acgtctttca ttatggttat
      781 tgttcatgag cgtgtgcctg acatgcagac ctacccacca ctcccagata tattcctaga
      841 cagtgttcct aggatcccgt gggccttctc catgaccgaa gtgtgtggcg tgattctgtg
      901 ctacatttgg atcctggttc ttcttcttca caagcacagg tcaatccttc tgcgaaggct
      961 ctgtagtctg atgggcactg tattcttgct tcgctgcttt accatgtttg tgacctccct
     1021 ctccgtgcca ggacagcacc tgcagtgtac tggaaagata tatggaagtg tgtgggagaa
     1081 actacgacgg gcatttgcca tctggagcgg ctttggaatg actctgactg gcgtccacac
     1141 ttgtggagac tacatgttca gtggccacac agttgttcta accatgctga atttctttgt
     1201 cacagagtat acaccaagaa gctggaattt cttgcacact ctatcctggg ttctcaacct
     1261 ctttggaatc ttcttcatct tggctgccca tgaacattat tccattgatg tgtttattgc
     1321 cttttatatc acaacaagac tctttttgta ctaccatact ctggctaaca ccagagcgta
     1381 tcatcagagt aggagagcga ggatttggtt ccctatgttt tctttctttg aatgcaatgt
     1441 taatggcaca gtacctaatg aatattgttg gccattttct aaaccagcaa taatgaaaag
     1501 actaattgga tgaagactct ctctctgtaa tgagtttgtt gttaaatata cagtagttat
     1561 ggaatgaaaa tgaatacttt gtcccaggct cctggatacg ttgcatttgg cttctgtgtt
     1621 gacaggtttc ccgttctgag aaaggccgtg actgtaaaac tcaagaaaga acacacaaga
     1681 ctccttatta gctgtttgaa tggagaggtc atggaaagag tttccgcccc tcactgtagg
     1741 aagacccagc gtggggtgat gtcaggccga tacctgtgca tgctgagtgt ttgcttacgg
     1801 aactcaagca catcagcttg ggctggttga agaattgtta ctgattccca gtaggactca
     1861 agtcaagaat atttctgaag ctggctttcc ttacctagtg agccgttctc atgtgttcat
     1921 tgaactaatc tgtgtctgtc tgcaaagtga gtgtcttact ggtaaatatg ttttaccttt
     1981 tgagccattt gaaagaaaaa cacattgaac acagcagaaa caaaagaatg cggggggcac
     2041 cgtgtgaggt gacacgctcg ctgatcaggt gtcgccggcc tcccactaat gcaggggtca
     2101 gcatttcaga gcaggtgcag cgtcagttgc tgtccctttc tgttcggaag cttcagtgtg
     2161 aacaacagaa agggctcctg aggggaggtg gctgtgttcc tgtagtgctg taagtccccg
     2221 gactaaatac ttgttgtaac tgttagttag gcagatgaca gcccaatata ctgcttttta
     2281 ataatgtgac ttaagtgaac acatgagtgt tttaccagat gttagagttc ttacatcagt
     2341 gagtgagtaa atgtgcatgc agagaattct tttcttctgg aaaaaaacaa aacagaacag
     2401 aacaagctgt aggcgcagtg ctaattctcg gggtgggggt gggggtgggg tcctcttttt
     2461 gttgttgttt ttccctgaaa gttctcattc ccgaccatgg aaagtacaca gtctgagaca
     2521 tgggtggata gaaaaaggct tcttactttt tattttaaaa aaattatata catatgttct
     2581 ttctagagtc ttaatttggc agttaagaac taaatacttt agctgctgtt taccttcagt
     2641 aattactgtc acctctccga taccatttgt cactttattt tctattggtt gtacataaca
     2701 gttttaacct ggttgtagaa ttggaatact aattgtatag tctttacgtt ccattttcaa
     2761 acaaggactc aagagctgca ggcaggcgca ctgcagtctc acgcaggcca cagccggcgt
     2821 ttattcctct gtctcggcgg ctgaatggtc atttttgcag ttggagtcat agtcccacct
     2881 cttggaagcc actgagtgga ttaggcagga agggggtttg tctaatagac aaacgggcct
     2941 cccccgtgct gagaacccct gctctgagga ggctggagac agcgctcatt tctcacctga
     3001 gagatgaggt aaagcaatca aaatcaagtt caattatgtt tttaaatctt gtacttcaaa
     3061 atccttgcag tattttaaat tattgattct attacaagcc aggatcaaaa acgccaatat
     3121 cttatctaaa gaaacgtcct aatcctggag aaaaggtttg ctatgcttgt ttgagtagct
     3181 tgacttaggg ccaagggata tattactgcc accccctctt attactcccc tgccccccac
     3241 agagcaggat ccaatttacc tggggggagg gggagagaca gtgttggctt ttgggaaaat
     3301 gttccaaaaa tgcgtgtcta cattagtttt aagatgctaa ctatattaat ggtaaatatc
     3361 ttaacatcag gcttctctcc tccatatggg ttctatcata gaacacaata ggaaacaggt
     3421 gcagctggcg ttgcccttgc caagctattg atggtgagag tgcctgacag cagcctgctg
     3481 tacggattga gtggccttgg gtcactcctg tttgtttgga ggaaaaagat aagctagatg
     3541 tatagcctag tagctactat aagacagtgt ttacagagct aatcctcttc aagtctcaaa
     3601 actgagacaa cattagtttt caggttttgt cctcacatct ttttccttac taaaacctaa
     3661 ccataatgaa gatagaattt atgtttacat tttgggcatg atgtggctgc gtgcagtctc
     3721 cacgcttttg aaccccgtat ttgattctgc agtcgtgtga gacttttctc cttgacagct
     3781 tagtacagaa gcaaggaaag gttttgagtt cttgggggtg caaaggaaga attgcctaca
     3841 ttctaatccc agtgtcgctg ccctccctgt ggtgttaagc ccagggctgt gtgtgcgagg
     3901 caagtgctct cggcttttac agtgcaaaca ggatctactg aaaggtagac atgctgggtg
     3961 gaaaggaacg agctcctatg gaacataagc ttgctctcac caccacatag ttttggcaga
     4021 aacctttgtg ttacacttcc caacgaggcc agagttgctg tggcaaaaat aacctttcta
     4081 aagcaatggg cttcatctta gaaccagtca atttaaataa cgtgagctgt aaccaagaaa
     4141 acccacactt ccaatagcgt aacattgaca ctgtaagtgg tgaccagccc cattctacct
     4201 gcctgttagg actgagaatt ccaaaactct gtaagttata agtggggaac aggggtgctg
     4261 gatccttcta tagcagagag caagtagtct ttcaggaaca tttgtgcaaa agtgtttggt
     4321 tgtttgttgg gggggggggg ttctttgttt ttgtttttgt tttattttgt tttgtttttt
     4381 cagagacagg gtttctctaa ttcagggatc acctgtcttt gcctcccatg ctgggattaa
     4441 aggtgtacac caccacacac atctttttgt aaaacttaat tcgtataaat tcagatttgt
     4501 ggcaaaaatg tatcttcttt atattcagca aaaagaaatt ttatcattga aagattctag
     4561 atccaaaatg cttaacaatg tttacaatat tcttgactta aactctctga tttagtacac
     4621 tcaattattt gatggtcttg tggatagaaa tcccatagca aaagcagaca gtatagtcgc
     4681 cctctcagct gacagactta ggagacaaga acggtcttta gaatagtcca cttgctttcc
     4741 ttcagacagc gtgaaagcag aaagcatgtc aggggttttt gttgttgttt ttttgttttc
     4801 tttttttttt tttttttttt ggtttggttt ggtttgggtt ttttgttatt gttggttgtg
     4861 ggttttgttt ttgttttcat tttaaacaag cttatgtaaa taagatgctt gacttgaagg
     4921 gaaaactatc taagatcatt ttgttcaaga gtaatttatt ccctaaagac atgtaacagc
     4981 ttcaaaaaaa atataaaaat tgtccttgaa gctgggcatg gtggagcacg cctttaatcc
     5041 cagcacttgg taggcagagg caggcggatt tctgagttca aggccagcct ggtctacaga
     5101 gtgagttcca ggacagccaa ggctacacag agaaaccctg tctcaaaaaa ccaaaaaaaa
     5161 aaaaaaattg tccttggttt tgcaatgata aatgaaaatc attaaaattc tccaattgaa
     5221 caaggcatgc aaggatttgg tggtgttgtg ttcttttgtt aaaacaatga gagcaaaata
     5281 acttactgaa atataaagat gagagctgaa tgagcatgcc accaatggag aaggggtatt
     5341 ttcacagacc cagtactttc ccccagacca tctccatctg atgccaacca gaacagaaca
     5401 ttggccattt cgtttaaaaa aaggaaaaga aagagagagc actatgcgtg tgcacataca
     5461 ccagttactt tttgtacaat aaagaaatgg ggacagggaa atgaaagaag agaaaactat
     5521 actgtgtagt caggatgtgg cgggaccaaa ctgtggcttt ttaattgaga atgtattctt
     5581 ggtctataac aaattctgga gtaaagtagc aggctccctt tttaagtaga cacctccagt
     5641 ctgctgctgg aacacataca tcgatcttta ttctccactg attggttgcc catcgaaccg
     5701 gaatctgatc tgcctcattg acaaaccctg ccgttcacaa taggctttca ttagtttact
     5761 aagtggtata tgcctcttaa ccttacactg caccacagaa ccatcctgtc ccgccacctt
     5821 caaattaata tgatcattgt tctcagtctt gactccttcc ttgggtttct cgtcagccat
     5881 ggcaagtgca ggagtctcct tgtggccttc gctgtcctgg aactcagaca tttttctgcc
     5941 tctgtctttg cctctgcctc tgcctcccaa gttgctggga ttaaaggcaa gcatcaccac
     6001 ttactgccta gcaagcatgt cagggttttt ttttgttttg tttttgtttg tttttttgag
     6061 acagggtttc tctgtatagc cctgactgtc ctgaaactca caagcatatc aggtttaatg
     6121 gcaactttac ataacagttt gtgtttatta aaaagtgaga ttagtcccag tcacccctat
     6181 tacctctgca gctgaggatt aaagtaccat cagagatgct tcaggctact ggatggaaag
     6241 agaaagggtt ttgtttgttt gttctcctta tgaatagctt taaatgacta aaattactgt
     6301 aaaagtaggt gttggtttgc ctgtagattc agtggcactg attttacata actaatatga
     6361 tgaattactg tatcaagttc agtacaaagg tctgtgaact ctaagctatt tctccagcta
     6421 cactaagttg tttttgagga tacattctac actgtgttaa tcaaaggact ctttattctg
     6481 tgaatttccc atcttggcat aaggaagctt tgtgctttga tgggagaagg aatattctat
     6541 tgtttgtttt cttttttcca ttgttacttc acatctcttt taatctgaac tagtggattg
     6601 tctgaagaac caaaccgagc ctattgttat taacatcttt aaaatgttta attttaataa
     6661 tttttcattg aattcatgga ttgttgggat aatgaagtat ttgtgatgtg gaacaaagga
     6721 aatgaaattg gtactcagtg gggaagcagg ttgcttggac taagccaggc atgttcagtt
     6781 ttgagaattc tacctaaagg tctccctgcc cacagagaaa ctagtgttac ttgaagacat
     6841 gaaaatccct gtggtagcca gaccttgaag cacagtgttg taataagtaa atacctgatt
     6901 ctaaattaaa ttcagattta actaatatat attattttat aactttgttg tattaaaatg
     6961 ttttaaacac taaaaaataa agcatttgaa agttggtaaa aaaaaaaaaa aaaaa
//
