LOCUS       NM_001359954            7071 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus sterile alpha motif domain containing 8 (Samd8),
            transcript variant 2, mRNA.
ACCESSION   NM_001359954 XM_006519459
VERSION     NM_001359954.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Chiang,Y.P., Li,Z., He,M., Jones,Q., Pan,M., Han,X. and Jiang,X.C.
  TITLE     Sphingomyelin synthase-related protein SMSr is a
            phosphatidylethanolamine phospholipase C that promotes nonalcoholic
            fatty liver disease
  JOURNAL   J Biol Chem 299 (9), 105162 (2023)
   PUBMED   37586586
  REMARK    GeneRIF: Sphingomyelin synthase-related protein SMSr is a
            phosphatidylethanolamine phospholipase C that promotes nonalcoholic
            fatty liver disease.
            Erratum:[J Biol Chem. 2024 Apr;300(4):107204. doi:
            10.1016/j.jbc.2024.107204.. PMID: 38552502]
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Bickert,A., Ginkel,C., Kol,M., vom Dorp,K., Jastrow,H., Degen,J.,
            Jacobs,R.L., Vance,D.E., Winterhager,E., Jiang,X.C., Dormann,P.,
            Somerharju,P., Holthuis,J.C. and Willecke,K.
  TITLE     Functional characterization of enzymes catalyzing ceramide
            phosphoethanolamine biosynthesis in mice
  JOURNAL   J Lipid Res 56 (4), 821-835 (2015)
   PUBMED   25667419
  REMARK    Erratum:[J Lipid Res. 2017 Apr;58(4):820. doi:
            10.1194/jlr.M055269ERR.. PMID: 28365667]
REFERENCE   3  (bases 1 to 7071)
  AUTHORS   Ding,T., Kabir,I., Li,Y., Lou,C., Yazdanyar,A., Xu,J., Dong,J.,
            Zhou,H., Park,T., Boutjdir,M., Li,Z. and Jiang,X.C.
  TITLE     All members in the sphingomyelin synthase gene family have ceramide
            phosphoethanolamine synthase activity
  JOURNAL   J Lipid Res 56 (3), 537-545 (2015)
   PUBMED   25605874
  REMARK    GeneRIF: study reveals that all mouse SMS family members (SMSr,
            SMS1, and SMS2) have CPE synthase activity
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151533.3, BY204342.1, BY148402.1, AK147528.1 and BQ176258.1.
            
            On Jan 24, 2018 this sequence version replaced XM_006519459.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and uses
            an alternate splice site in the 5' coding region, compared to
            variant 1. The encoded isoform (2) has a shorter and distinct
            N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.13256.1,
                                           SRR10223970.16698.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC151533.3         81302-81323         c
            23-28               BY204342.1         2-7
            29-365              BY148402.1         1-337
            366-4088            AK147528.1         114-3836
            4089-4089           AC151533.3         36093-36093         c
            4090-4395           AK147528.1         3838-4143
            4396-4397           AC151533.3         35785-35786         c
            4398-5091           AK147528.1         4147-4840
            5092-5092           AC151533.3         35090-35090         c
            5093-5455           AK147528.1         4842-5204
            5456-5456           AC151533.3         34726-34726         c
            5457-6038           AK147528.1         5206-5787
            6039-6039           AC151533.3         34143-34143         c
            6040-6089           AK147528.1         5789-5838
            6090-6090           AC151533.3         34092-34092         c
            6091-7053           AK147528.1         5839-6801
            7054-7071           BQ176258.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 11.86 cM"
     gene            1..7071
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="sterile alpha motif domain containing 8"
                     /db_xref="GeneID:67630"
                     /db_xref="MGI:MGI:1914880"
     exon            1..306
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     CDS             271..1569
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /EC_number="2.7.8.48"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sphingomyelin synthase-related protein 1; GrailEXP
                     prediction; CPE synthase; SAM domain-containing protein 8;
                     ceramide phosphoethanolamine synthase; sterile alpha motif
                     domain-containing protein 8"
                     /codon_start=1
                     /product="sphingomyelin synthase-related protein 1 isoform
                     2"
                     /protein_id="NP_001346883.1"
                     /db_xref="GeneID:67630"
                     /db_xref="MGI:MGI:1914880"
                     /translation="MRGEIGGRLQPREVEKEMAGPSQLCIRRWTTKHVAVWLKDEGFF
                     EYVDILCNKHRLDGITLLTLTEYDLRSPPLEIKVLGDIKRLMLSVRKLQKIHTDVLEE
                     MGYNSDSPMSPMTPFLSALQSADWLCNGEPTHSCDGPIPDLSSDQYQYMNGKNKHSAR
                     RLDPEYWKTILSCVYVFIVFGFTSFIMVIVHERVPDMQTYPPLPDIFLDSVPRIPWAF
                     SMTEVCGVILCYIWILVLLLHKHRSILLRRLCSLMGTVFLLRCFTMFVTSLSVPGQHL
                     QCTGKIYGSVWEKLRRAFAIWSGFGMTLTGVHTCGDYMFSGHTVVLTMLNFFVTEYTP
                     RSWNFLHTLSWVLNLFGIFFILAAHEHYSIDVFIAFYITTRLFLYYHTLANTRAYHQS
                     RRARIWFPMFSFFECNVNGTVPNEYCWPFSKPAIMKRLIG"
     exon            307..899
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            900..995
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1113
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1264
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..7054
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1601..1606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="hexamer: AATATA"
     polyA_site      1630
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="major polyA site"
     regulatory      7032..7037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="hexamer: AATAAA"
     polyA_site      7054
                     /gene="Samd8"
                     /gene_synonym="1110053F04Rik; 1700010P07Rik; SMSr"
                     /note="major polyA site"
ORIGIN      
        1 gtagcgcccc gcctcccgaa gcagttccgg ttcggctggc cgctgctggc ggcacggctc
       61 caaaggggcc ggagcgatgc ccgctggtag ccgagccggc tcccgcctgc gctccggctc
      121 cctcccccgg ccttcgcgtc tgactctcaa ggcgctgcgc cccgcctacg cgccgaggac
      181 ccccgactcc aacggcgact tagacaccgg ctccgagctg ggtcccggca gcccggctcc
      241 caccgcggag gtgagcgggc gccgaggctg atgagagggg agattggggg gcgcctgcaa
      301 ccaagggaag tagagaaaga aatggcaggt cctagtcaac tctgcattcg ccgctggact
      361 accaagcatg tggctgtgtg gctgaaggac gaaggtttct ttgagtatgt ggacatttta
      421 tgcaacaagc accgccttga tggaatcaca ttgctcacgc tgactgaata tgatctccgc
      481 tctcctcctc tggaaatcaa agtcctagga gacattaaac ggttaatgct ctcagtccga
      541 aaattgcaga aaatacatac tgatgttttg gaagagatgg gctacaacag tgacagtcct
      601 atgagtccca tgactccatt ccttagtgct cttcagagtg cagactggct ctgtaacgga
      661 gagcccacac acagctgtga tgggcccatc cctgacttga gttctgatca gtaccagtac
      721 atgaatggca aaaacaaaca ttctgctcga agactggacc cagagtactg gaagaccatc
      781 ctgagttgtg tatatgtttt tatagtattt gggtttacgt ctttcattat ggttattgtt
      841 catgagcgtg tgcctgacat gcagacctac ccaccactcc cagatatatt cctagacagt
      901 gttcctagga tcccgtgggc cttctccatg accgaagtgt gtggcgtgat tctgtgctac
      961 atttggatcc tggttcttct tcttcacaag cacaggtcaa tccttctgcg aaggctctgt
     1021 agtctgatgg gcactgtatt cttgcttcgc tgctttacca tgtttgtgac ctccctctcc
     1081 gtgccaggac agcacctgca gtgtactgga aagatatatg gaagtgtgtg ggagaaacta
     1141 cgacgggcat ttgccatctg gagcggcttt ggaatgactc tgactggcgt ccacacttgt
     1201 ggagactaca tgttcagtgg ccacacagtt gttctaacca tgctgaattt ctttgtcaca
     1261 gagtatacac caagaagctg gaatttcttg cacactctat cctgggttct caacctcttt
     1321 ggaatcttct tcatcttggc tgcccatgaa cattattcca ttgatgtgtt tattgccttt
     1381 tatatcacaa caagactctt tttgtactac catactctgg ctaacaccag agcgtatcat
     1441 cagagtagga gagcgaggat ttggttccct atgttttctt tctttgaatg caatgttaat
     1501 ggcacagtac ctaatgaata ttgttggcca ttttctaaac cagcaataat gaaaagacta
     1561 attggatgaa gactctctct ctgtaatgag tttgttgtta aatatacagt agttatggaa
     1621 tgaaaatgaa tactttgtcc caggctcctg gatacgttgc atttggcttc tgtgttgaca
     1681 ggtttcccgt tctgagaaag gccgtgactg taaaactcaa gaaagaacac acaagactcc
     1741 ttattagctg tttgaatgga gaggtcatgg aaagagtttc cgcccctcac tgtaggaaga
     1801 cccagcgtgg ggtgatgtca ggccgatacc tgtgcatgct gagtgtttgc ttacggaact
     1861 caagcacatc agcttgggct ggttgaagaa ttgttactga ttcccagtag gactcaagtc
     1921 aagaatattt ctgaagctgg ctttccttac ctagtgagcc gttctcatgt gttcattgaa
     1981 ctaatctgtg tctgtctgca aagtgagtgt cttactggta aatatgtttt accttttgag
     2041 ccatttgaaa gaaaaacaca ttgaacacag cagaaacaaa agaatgcggg gggcaccgtg
     2101 tgaggtgaca cgctcgctga tcaggtgtcg ccggcctccc actaatgcag gggtcagcat
     2161 ttcagagcag gtgcagcgtc agttgctgtc cctttctgtt cggaagcttc agtgtgaaca
     2221 acagaaaggg ctcctgaggg gaggtggctg tgttcctgta gtgctgtaag tccccggact
     2281 aaatacttgt tgtaactgtt agttaggcag atgacagccc aatatactgc tttttaataa
     2341 tgtgacttaa gtgaacacat gagtgtttta ccagatgtta gagttcttac atcagtgagt
     2401 gagtaaatgt gcatgcagag aattcttttc ttctggaaaa aaacaaaaca gaacagaaca
     2461 agctgtaggc gcagtgctaa ttctcggggt gggggtgggg gtggggtcct ctttttgttg
     2521 ttgtttttcc ctgaaagttc tcattcccga ccatggaaag tacacagtct gagacatggg
     2581 tggatagaaa aaggcttctt actttttatt ttaaaaaaat tatatacata tgttctttct
     2641 agagtcttaa tttggcagtt aagaactaaa tactttagct gctgtttacc ttcagtaatt
     2701 actgtcacct ctccgatacc atttgtcact ttattttcta ttggttgtac ataacagttt
     2761 taacctggtt gtagaattgg aatactaatt gtatagtctt tacgttccat tttcaaacaa
     2821 ggactcaaga gctgcaggca ggcgcactgc agtctcacgc aggccacagc cggcgtttat
     2881 tcctctgtct cggcggctga atggtcattt ttgcagttgg agtcatagtc ccacctcttg
     2941 gaagccactg agtggattag gcaggaaggg ggtttgtcta atagacaaac gggcctcccc
     3001 cgtgctgaga acccctgctc tgaggaggct ggagacagcg ctcatttctc acctgagaga
     3061 tgaggtaaag caatcaaaat caagttcaat tatgttttta aatcttgtac ttcaaaatcc
     3121 ttgcagtatt ttaaattatt gattctatta caagccagga tcaaaaacgc caatatctta
     3181 tctaaagaaa cgtcctaatc ctggagaaaa ggtttgctat gcttgtttga gtagcttgac
     3241 ttagggccaa gggatatatt actgccaccc cctcttatta ctcccctgcc ccccacagag
     3301 caggatccaa tttacctggg gggaggggga gagacagtgt tggcttttgg gaaaatgttc
     3361 caaaaatgcg tgtctacatt agttttaaga tgctaactat attaatggta aatatcttaa
     3421 catcaggctt ctctcctcca tatgggttct atcatagaac acaataggaa acaggtgcag
     3481 ctggcgttgc ccttgccaag ctattgatgg tgagagtgcc tgacagcagc ctgctgtacg
     3541 gattgagtgg ccttgggtca ctcctgtttg tttggaggaa aaagataagc tagatgtata
     3601 gcctagtagc tactataaga cagtgtttac agagctaatc ctcttcaagt ctcaaaactg
     3661 agacaacatt agttttcagg ttttgtcctc acatcttttt ccttactaaa acctaaccat
     3721 aatgaagata gaatttatgt ttacattttg ggcatgatgt ggctgcgtgc agtctccacg
     3781 cttttgaacc ccgtatttga ttctgcagtc gtgtgagact tttctccttg acagcttagt
     3841 acagaagcaa ggaaaggttt tgagttcttg ggggtgcaaa ggaagaattg cctacattct
     3901 aatcccagtg tcgctgccct ccctgtggtg ttaagcccag ggctgtgtgt gcgaggcaag
     3961 tgctctcggc ttttacagtg caaacaggat ctactgaaag gtagacatgc tgggtggaaa
     4021 ggaacgagct cctatggaac ataagcttgc tctcaccacc acatagtttt ggcagaaacc
     4081 tttgtgttac acttcccaac gaggccagag ttgctgtggc aaaaataacc tttctaaagc
     4141 aatgggcttc atcttagaac cagtcaattt aaataacgtg agctgtaacc aagaaaaccc
     4201 acacttccaa tagcgtaaca ttgacactgt aagtggtgac cagccccatt ctacctgcct
     4261 gttaggactg agaattccaa aactctgtaa gttataagtg gggaacaggg gtgctggatc
     4321 cttctatagc agagagcaag tagtctttca ggaacatttg tgcaaaagtg tttggttgtt
     4381 tgttgggggg ggggggttct ttgtttttgt ttttgtttta ttttgttttg ttttttcaga
     4441 gacagggttt ctctaattca gggatcacct gtctttgcct cccatgctgg gattaaaggt
     4501 gtacaccacc acacacatct ttttgtaaaa cttaattcgt ataaattcag atttgtggca
     4561 aaaatgtatc ttctttatat tcagcaaaaa gaaattttat cattgaaaga ttctagatcc
     4621 aaaatgctta acaatgttta caatattctt gacttaaact ctctgattta gtacactcaa
     4681 ttatttgatg gtcttgtgga tagaaatccc atagcaaaag cagacagtat agtcgccctc
     4741 tcagctgaca gacttaggag acaagaacgg tctttagaat agtccacttg ctttccttca
     4801 gacagcgtga aagcagaaag catgtcaggg gtttttgttg ttgttttttt gttttctttt
     4861 tttttttttt ttttttggtt tggtttggtt tgggtttttt gttattgttg gttgtgggtt
     4921 ttgtttttgt tttcatttta aacaagctta tgtaaataag atgcttgact tgaagggaaa
     4981 actatctaag atcattttgt tcaagagtaa tttattccct aaagacatgt aacagcttca
     5041 aaaaaaatat aaaaattgtc cttgaagctg ggcatggtgg agcacgcctt taatcccagc
     5101 acttggtagg cagaggcagg cggatttctg agttcaaggc cagcctggtc tacagagtga
     5161 gttccaggac agccaaggct acacagagaa accctgtctc aaaaaaccaa aaaaaaaaaa
     5221 aaattgtcct tggttttgca atgataaatg aaaatcatta aaattctcca attgaacaag
     5281 gcatgcaagg atttggtggt gttgtgttct tttgttaaaa caatgagagc aaaataactt
     5341 actgaaatat aaagatgaga gctgaatgag catgccacca atggagaagg ggtattttca
     5401 cagacccagt actttccccc agaccatctc catctgatgc caaccagaac agaacattgg
     5461 ccatttcgtt taaaaaaagg aaaagaaaga gagagcacta tgcgtgtgca catacaccag
     5521 ttactttttg tacaataaag aaatggggac agggaaatga aagaagagaa aactatactg
     5581 tgtagtcagg atgtggcggg accaaactgt ggctttttaa ttgagaatgt attcttggtc
     5641 tataacaaat tctggagtaa agtagcaggc tcccttttta agtagacacc tccagtctgc
     5701 tgctggaaca catacatcga tctttattct ccactgattg gttgcccatc gaaccggaat
     5761 ctgatctgcc tcattgacaa accctgccgt tcacaatagg ctttcattag tttactaagt
     5821 ggtatatgcc tcttaacctt acactgcacc acagaaccat cctgtcccgc caccttcaaa
     5881 ttaatatgat cattgttctc agtcttgact ccttccttgg gtttctcgtc agccatggca
     5941 agtgcaggag tctccttgtg gccttcgctg tcctggaact cagacatttt tctgcctctg
     6001 tctttgcctc tgcctctgcc tcccaagttg ctgggattaa aggcaagcat caccacttac
     6061 tgcctagcaa gcatgtcagg gttttttttt gttttgtttt tgtttgtttt tttgagacag
     6121 ggtttctctg tatagccctg actgtcctga aactcacaag catatcaggt ttaatggcaa
     6181 ctttacataa cagtttgtgt ttattaaaaa gtgagattag tcccagtcac ccctattacc
     6241 tctgcagctg aggattaaag taccatcaga gatgcttcag gctactggat ggaaagagaa
     6301 agggttttgt ttgtttgttc tccttatgaa tagctttaaa tgactaaaat tactgtaaaa
     6361 gtaggtgttg gtttgcctgt agattcagtg gcactgattt tacataacta atatgatgaa
     6421 ttactgtatc aagttcagta caaaggtctg tgaactctaa gctatttctc cagctacact
     6481 aagttgtttt tgaggataca ttctacactg tgttaatcaa aggactcttt attctgtgaa
     6541 tttcccatct tggcataagg aagctttgtg ctttgatggg agaaggaata ttctattgtt
     6601 tgttttcttt tttccattgt tacttcacat ctcttttaat ctgaactagt ggattgtctg
     6661 aagaaccaaa ccgagcctat tgttattaac atctttaaaa tgtttaattt taataatttt
     6721 tcattgaatt catggattgt tgggataatg aagtatttgt gatgtggaac aaaggaaatg
     6781 aaattggtac tcagtgggga agcaggttgc ttggactaag ccaggcatgt tcagttttga
     6841 gaattctacc taaaggtctc cctgcccaca gagaaactag tgttacttga agacatgaaa
     6901 atccctgtgg tagccagacc ttgaagcaca gtgttgtaat aagtaaatac ctgattctaa
     6961 attaaattca gatttaacta atatatatta ttttataact ttgttgtatt aaaatgtttt
     7021 aaacactaaa aaataaagca tttgaaagtt ggtaaaaaaa aaaaaaaaaa a
//
