LOCUS       NM_001115154            2577 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus sterile alpha motif domain containing 3 (Samd3),
            transcript variant 2, mRNA.
ACCESSION   NM_001115154
VERSION     NM_001115154.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Peters,A.E., Knopper,K., Grafen,A. and Kastenmuller,W.
  TITLE     A multifunctional mouse model to study the role of Samd3
  JOURNAL   Eur J Immunol 52 (2), 328-337 (2022)
   PUBMED   34626120
  REMARK    GeneRIF: A multifunctional mouse model to study the role of Samd3.
REFERENCE   3  (bases 1 to 2577)
  AUTHORS   Moreau,M.X., Saillour,Y., Cwetsch,A.W., Pierani,A. and Causeret,F.
  TITLE     Single-cell transcriptomics of the early developing mouse cerebral
            cortex disentangle the spatial and temporal components of neuronal
            fate acquisition
  JOURNAL   Development 148 (14) (2021)
   PUBMED   34170322
REFERENCE   4  (bases 1 to 2577)
  AUTHORS   Loo,L., Simon,J.M., Xing,L., McCoy,E.S., Niehaus,J.K., Guo,J.,
            Anton,E.S. and Zylka,M.J.
  TITLE     Single-cell transcriptomic analysis of mouse neocortical
            development
  JOURNAL   Nat Commun 10 (1), 134 (2019)
   PUBMED   30635555
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2577)
  AUTHORS   Quintana-Urzainqui,I., Kozic,Z., Mitra,S., Tian,T., Manuel,M.,
            Mason,J.O. and Price,D.J.
  TITLE     Tissue-Specific Actions of Pax6 on Proliferation and
            Differentiation Balance in Developing Forebrain Are Foxg1 Dependent
  JOURNAL   iScience 10, 171-191 (2018)
   PUBMED   30529950
REFERENCE   6  (bases 1 to 2577)
  AUTHORS   Kashiwagi,I., Morita,R., Schichita,T., Komai,K., Saeki,K.,
            Matsumoto,M., Takeda,K., Nomura,M., Hayashi,A., Kanai,T. and
            Yoshimura,A.
  TITLE     Smad2 and Smad3 Inversely Regulate TGF-beta Autoinduction in
            Clostridium butyricum-Activated Dendritic Cells
  JOURNAL   Immunity 43 (1), 65-79 (2015)
   PUBMED   26141582
REFERENCE   7  (bases 1 to 2577)
  AUTHORS   Visel,A., Carson,J., Oldekamp,J., Warnecke,M., Jakubcakova,V.,
            Zhou,X., Shaw,C.A., Alvarez-Bolado,G. and Eichele,G.
  TITLE     Regulatory pathway analysis by high-throughput in situ
            hybridization
  JOURNAL   PLoS Genet 3 (10), 1867-1883 (2007)
   PUBMED   17953485
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC091763.25.
            
            On Jun 14, 2022 this sequence version replaced NM_001115154.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.797400.1,
                                           SRR7345562.4032860.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AC091763.25        81330-81396
            68-168              AC091763.25        81686-81786
            169-358             AC091763.25        82410-82599
            359-460             AC091763.25        85108-85209
            461-646             AC091763.25        96063-96248
            647-731             AC091763.25        97126-97210
            732-899             AC091763.25        103331-103498
            900-1100            AC091763.25        115364-115564
            1101-1272           AC091763.25        121672-121843
            1273-1355           AC091763.25        122988-123070
            1356-2577           AC091763.25        123277-124498
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 13.57 cM"
     gene            1..2577
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /note="sterile alpha motif domain containing 3"
                     /db_xref="GeneID:268288"
                     /db_xref="MGI:MGI:2685469"
     exon            1..67
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            68..168
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..1634
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sterile alpha motif domain-containing protein 3; SAM
                     domain-containing protein 3"
                     /codon_start=1
                     /product="sterile alpha motif domain-containing protein 3
                     isoform 2"
                     /protein_id="NP_001108626.1"
                     /db_xref="CCDS:CCDS48525.1"
                     /db_xref="GeneID:268288"
                     /db_xref="MGI:MGI:2685469"
                     /translation="METWSVDQVCKWLVEKNLGELVPRFQEEEVSGATLLALNDRMVQ
                     QLVKKIGHQAVLMDFIKKYKQGNQELKPTGGPADTSTLTPAQAAPEHEQNPSPTSHGD
                     QTSLYPAVLDNRLIDQRVLKQRRNVKHVLARHKALQWTKSYILPEFPYDVKCMLVEQK
                     RPDHSMRIRIIEFLQADMTKYLEGSLYPTTQQYNDVVNALLQAHPFLDEDGCGFFLWK
                     RALKDRFKYIRRPIEDDEQVMRNKCKFGHRRGQTRKSLADIQSNEIKIVQIKEESAHL
                     DSEVDEHISWFQQEYMKTERDWREVDKRMSQTLEIRRKMIGGQTPLKDILKMFPFLKC
                     PYQMFREVQILTKTDIYKKTRHILESYSENILTAFSVLDNPINTALQEKMKHYTDEGV
                     LKYMKMTATCLLLPHVFGDEPSLFVVVNGKVHVSTPVLEVKNPFHINGCEFSLYLNKE
                     KLTKVDDCVTALAALVSAFRVFGIECPRRLSQTFNFLETLIFDMQSPQFPSLKEKEIR
                     SQPPIT"
     exon            169..358
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            359..460
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            461..646
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            647..731
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            732..899
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1100
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1272
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1355
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..2577
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1812..1817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /note="hexamer: AATAAA"
     polyA_site      1838
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /note="major polyA site"
     regulatory      2556..2561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Samd3"
                     /gene_synonym="Gm623"
                     /note="hexamer: AATAAA"
     polyA_site      2577
                     /gene="Samd3"
                     /gene_synonym="Gm623"
ORIGIN      
        1 accattgtat gtgccggctg tctgctattg aagctgacgg atgcctgcgg aaatcaagta
       61 ggagaaggtg tattgacaag aatccagcaa tggaaacgtg gtcagttgat caggtctgca
      121 aatggttggt ggaaaaaaac ttaggtgagc tagtccccag gtttcaagag gaagaagtca
      181 gtggggccac actcctggcc cttaatgatc ggatggtaca gcaactggta aagaaaattg
      241 gacaccaggc tgttctgatg gacttcatta agaaatacaa gcagggcaat caagagctga
      301 agcctacggg aggccccgca gatacatcca cgctgacccc ggcacaagcg gcccctgagc
      361 acgaacaaaa ccctagtccc accagccacg gggaccagac atctttgtat ccagccgtcc
      421 ttgacaatag actcattgat caaagagtat tgaagcagag aagaaatgta aaacatgttc
      481 tagcaaggca taaagcatta cagtggacta agtcctacat cttaccggag tttccctatg
      541 atgtcaagtg catgttggtg gagcagaagc gccccgacca cagcatgcgg ataaggatca
      601 ttgagtttct ccaggcagat atgactaagt acctggaagg ctcgctgtac cccaccaccc
      661 agcagtacaa cgacgtggtc aatgctctgc tgcaggccca ccctttcctg gatgaggacg
      721 gctgtggctt ttttttatgg aaacgagccc tcaaagatcg ctttaaatat attcgaagac
      781 ccatagaaga tgatgaacaa gtaatgagga ataagtgtaa atttggacac cgaagaggcc
      841 agacaaggaa gtctcttgct gacatacaat cgaatgaaat taaaattgtg cagatcaaag
      901 aagaatctgc tcatttagac tcggaggtgg atgaacacat cagctggttc cagcaagagt
      961 atatgaaaac agaaagggac tggagagaag ttgacaagag gatgagccag actttggaaa
     1021 taagaagaaa gatgattggc ggccaaacac ctctcaagga cattcttaag atgtttcctt
     1081 tcctgaagtg cccttaccag atgttcagag aagttcaaat tcttacaaaa acagatattt
     1141 ataagaaaac aaggcatatc ctggaatcct actcggaaaa tatactgact gccttttcag
     1201 tgctggacaa tccaatcaat actgccctgc aagagaagat gaaacactac actgacgaag
     1261 gggtgctgaa gtacatgaag atgactgcca cgtgtttact ccttccacat gtttttgggg
     1321 atgagcccag cctttttgtc gttgtgaatg ggaaggtgca cgtgtccacg cctgtgttgg
     1381 aagttaaaaa ccctttccac atcaatggct gtgagttttc tctgtatttg aacaaagaga
     1441 agctcacaaa ggtggatgac tgtgtcacag ctctggctgc tttggtgtct gctttccgtg
     1501 tgtttgggat cgagtgtcca agaagactgt cccaaacctt caacttccta gagacattga
     1561 tctttgacat gcagagtccc cagtttccct ctttgaaaga aaaggaaatc agatctcaac
     1621 ctccaatcac ttaagaacaa ctgcatgaac tctctagggt gacagaagct tcatcaacaa
     1681 ttgcagattc atcttagaat atctattgcc ttggaggtag gggttagaaa agtcatgttg
     1741 tctccagcat caatgtattt ttgaagcctc tctctttaac ttagatattt aagttctttg
     1801 tgaagttccg taataaaatg ctacttaaag gttctcaatt tgatttgagt tctgtagtca
     1861 aggtttcatt ttcaacagtg tcagttttac ctggcataaa agacattatg aggtaaaatc
     1921 ctcccagtta gggtctgacc ctaaacttaa ctcagtgtga ctgcttgaaa gaccatgagt
     1981 tgaacagccg cctctttagt ttctttggtt agagcattgg ttggaaacat cttgaacttg
     2041 agtattttaa agagatatat agtcctcaat ttccttatcc cagacacact gtttggtttg
     2101 atgtgttttg gttttcttca atcaatattg aaaaaatcaa tagtgagaga gcagctgcaa
     2161 tcttgggata tcctccttgt aggccatctt gttggcacaa ggttctttcc atagagactc
     2221 agcccgtgct ccagggtcac actgaaattt gtttgttcat tcatgaaatg gtgagacagt
     2281 actacttctg gaataacagg acctaggcac tatctgtttt ctgaggactt tcacaagaaa
     2341 aagaaactgt aaaatagtag catgacttga tattatcatc aaaatatcat gttatttcct
     2401 aattctgaat gataggggtg gagttggggt gtgtgtgtgg ggtcattctg catattgtag
     2461 agattttaat gtgttaatac ttgtgacaca atcagataag cctcatttat ttcatcaaat
     2521 ggacttttta aaatatttat tttccttttt actataataa agtaacccct ttacaaa
//
