LOCUS       NM_030692               3580 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus SAC1 suppressor of actin mutations 1-like (yeast)
            (Sacm1l), transcript variant 1, mRNA.
ACCESSION   NM_030692
VERSION     NM_030692.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Zheng,J., Deng,Y., Wei,Z., Zou,H., Wen,X., Cai,J., Zhang,S.,
            Jia,B., Lu,M., Lu,K. and Lin,Y.
  TITLE     Lipid phosphatase SAC1 suppresses hepatitis B virus replication
            through promoting autophagic degradation of virions
  JOURNAL   Antiviral Res 213, 105601 (2023)
   PUBMED   37068596
  REMARK    GeneRIF: Lipid phosphatase SAC1 suppresses hepatitis B virus
            replication through promoting autophagic degradation of virions.
REFERENCE   3  (bases 1 to 3580)
  AUTHORS   Walch,P., Selkrig,J., Knodler,L.A., Rettel,M., Stein,F.,
            Fernandez,K., Vieitez,C., Potel,C.M., Scholzen,K., Geyer,M.,
            Rottner,K., Steele-Mortimer,O., Savitski,M.M., Holden,D.W. and
            Typas,A.
  TITLE     Global mapping of Salmonella enterica-host protein-protein
            interactions during infection
  JOURNAL   Cell Host Microbe 29 (8), 1316-1332 (2021)
   PUBMED   34237247
REFERENCE   4  (bases 1 to 3580)
  AUTHORS   Casas,M., Fado,R., Dominguez,J.L., Roig,A., Kaku,M., Chohnan,S.,
            Sole,M., Unzeta,M., Minano-Molina,A.J., Rodriguez-Alvarez,J.,
            Dickson,E.J. and Casals,N.
  TITLE     Sensing of nutrients by CPT1C controls SAC1 activity to regulate
            AMPA receptor trafficking
  JOURNAL   J Cell Biol 219 (10) (2020)
   PUBMED   32931550
  REMARK    GeneRIF: Sensing of nutrients by CPT1C controls SAC1 activity to
            regulate AMPA receptor trafficking.
REFERENCE   5  (bases 1 to 3580)
  AUTHORS   Nigorikawa,K., Matsumura,T., Sakamoto,H., Morioka,S., Kofuji,S.,
            Takasuga,S. and Hazeki,K.
  TITLE     Sac1 Phosphoinositide Phosphatase Regulates Foam Cell Formation by
            Modulating SR-A Expression in Macrophages
  JOURNAL   Biol Pharm Bull 42 (6), 923-928 (2019)
   PUBMED   31155588
  REMARK    GeneRIF: Glycosylation of SR-A was largely attenuated by Sac1
            knockdown, but neither mRNA expression nor protein degradation of
            SR-A were affected. These results suggest that Sac1 maintains SR-A
            protein levels by modulating SR-A glycosylation.
REFERENCE   6  (bases 1 to 3580)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   7  (bases 1 to 3580)
  AUTHORS   Hammond,G.R., Machner,M.P. and Balla,T.
  TITLE     A novel probe for phosphatidylinositol 4-phosphate reveals multiple
            pools beyond the Golgi
  JOURNAL   J Cell Biol 205 (1), 113-126 (2014)
   PUBMED   24711504
REFERENCE   8  (bases 1 to 3580)
  AUTHORS   Liu,Y., Boukhelifa,M., Tribble,E., Morin-Kensicki,E., Uetrecht,A.,
            Bear,J.E. and Bankaitis,V.A.
  TITLE     The Sac1 phosphoinositide phosphatase regulates Golgi membrane
            morphology and mitotic spindle organization in mammals
  JOURNAL   Mol Biol Cell 19 (7), 3080-3096 (2008)
   PUBMED   18480408
  REMARK    GeneRIF: Sac1 executes an essential household function in mammals
            that involves organization of both Golgi membranes and mitotic
            spindles
REFERENCE   9  (bases 1 to 3580)
  AUTHORS   Blagoveshchenskaya,A., Cheong,F.Y., Rohde,H.M., Glover,G.,
            Knodler,A., Nicolson,T., Boehmelt,G. and Mayinger,P.
  TITLE     Integration of Golgi trafficking and growth factor signaling by the
            lipid phosphatase SAC1
  JOURNAL   J Cell Biol 180 (4), 803-812 (2008)
   PUBMED   18299350
  REMARK    GeneRIF: These results suggest direct roles for p38 MAPK and SAC1
            in transmitting growth signals to the secretory machinery.
REFERENCE   10 (bases 1 to 3580)
  AUTHORS   Kiss,H., Darai,E., Kiss,C., Kost-Alimova,M., Klein,G.,
            Dumanski,J.P. and Imreh,S.
  TITLE     Comparative human/murine sequence analysis of the common eliminated
            region 1 from human 3p21.3
  JOURNAL   Mamm Genome 13 (11), 646-655 (2002)
   PUBMED   12461651
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY127420.1, CJ173186.1,
            AK161111.1, CN539161.1 and CO044347.1.
            
            On Oct 27, 2017 this sequence version replaced NM_030692.4.
            
            Summary: This gene encodes an integral membrane protein, which is
            localized to the endoplasmic reticulum, and functions as a
            phosphoinositide phosphatase that hydrolyzes phosphatidylinositol
            3-phosphate, phosphatidylinositol 4-phosphate, and
            phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in
            mouse results in preimplantation lethality. Other studies suggest
            that this gene is also involved in the organization of golgi
            membranes and mitotic spindles. Two isoforms are predicted to be
            produced from the same mRNA by the use of alternative in-frame
            translation termination codons via a stop codon readthrough
            mechanism. [provided by RefSeq, Dec 2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UGA termination codon. This
            RefSeq represents the shorter isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154759.1, SRR1660817.136215.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                BY127420.1         1-78
            79-96               CJ173186.1         44-61
            97-2671             AK161111.1         2-2576
            2672-3129           CN539161.1         163-620
            3130-3580           CO044347.1         1-451               c
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 74.08 cM"
     gene            1..3580
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /note="SAC1 suppressor of actin mutations 1-like (yeast)"
                     /db_xref="GeneID:83493"
                     /db_xref="MGI:MGI:1933169"
     exon            1..169
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..1901
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /EC_number="3.1.3.64"
                     /note="isoform 1 is encoded by transcript variant 1;
                     polyphosphatidylinositol phosphatase;
                     phosphatidylinositide phosphatase SAC1; suppressor of
                     actin mutations 1-like protein;
                     phosphatidylinositol-4-phosphate phosphatase;
                     phosphatidylinositol-3-phosphatase SAC1"
                     /codon_start=1
                     /product="phosphatidylinositol-3-phosphatase SAC1 isoform
                     1"
                     /protein_id="NP_109617.1"
                     /db_xref="CCDS:CCDS40816.1"
                     /db_xref="GeneID:83493"
                     /db_xref="MGI:MGI:1933169"
                     /translation="MAAAAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAV
                     KKDVPPSAVTRPIFGILGTIHLVAGNYLVVITKKMKVGECFNHAVWRATDFDVLSYKK
                     TMLHLTDIQLQDNKTFLAMLNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLE
                     RADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFR
                     AGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHP
                     QISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIR
                     YIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDC
                     LDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKTYKNAWADNANACAKQYA
                     GTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGFRQDSIDLFLGNYSVDELE
                     SHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFI
                     ILYNGKDFVDAPRLVQKEKID"
     exon            170..267
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            268..342
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            343..470
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            471..620
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            621..680
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            681..714
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            715..816
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            817..902
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            903..989
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1058
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1138
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1237
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1376
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1446
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1519
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1613
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1706
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1764
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..3564
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3540..3545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /note="hexamer: AATAAA"
     polyA_site      3564
                     /gene="Sacm1l"
                     /gene_synonym="mKIAA0851; SAC1; Sac1p"
                     /note="major polyA site"
ORIGIN      
        1 tcgcccgaga ggcggacccg ggagggggtg gggccaggcc gaggctcctt gaccaccgcg
       61 cctggggagc ggtcgccgcg gagccggggt ggcagcgggc tacactgcgg gtggagcgaa
      121 gaggaggaag ctgcacgatg gcggccgcag cctacgagca tctgaagctg catattacac
      181 ctgaaaagtt ttatgtggag gcttgtgatg atggagcaga tgatgtactc atcattgacc
      241 gagtgtccac agaggtcacc cttgcagtca aaaaagatgt tcccccctct gctgttacaa
      301 gaccaatatt tgggatactg ggcacaatcc atctggtggc aggtaattat cttgttgtca
      361 tcaccaaaaa gatgaaagta ggtgaatgtt ttaatcatgc ggtctggaga gccacagatt
      421 ttgatgttct ttcttataag aagacaatgt tgcacttgac tgatattcag ttacaagata
      481 ataaaacttt cctagcaatg ctaaaccatg tcttgagtat ggatggattt tacttttcaa
      541 caacatatga cttgactcat actttgcagc gactatctaa cacgagtcca gaattccaag
      601 aaatgagtct cttagaaagg gcagatcagc ggtttgtatg gaacgggcat ctgctcaggg
      661 aactgtctgc tcagccagag gtccatcggt ttgctcttcc agtgttgcat ggctttatta
      721 ctatgcactc atgttctatt aatggaaaat attttgattg gattctcatc tcccgaagga
      781 gttgtttcag agctggtgtt cgttattatg tgagaggaat tgattctgaa ggccacgcag
      841 ctaactttgt agaaacagag cagattgtgc actacagcgg gaacagggct tcgtttgtcc
      901 agactcgagg atcaatacct attttctggt cacagagacc aaatctaaag tacaaaccac
      961 atccacagat cagcaaagta gcaaatcaca tggatggttt ccagaggcat tttgattcac
     1021 aagtaattat ttatggaaag caagtaataa ttaatctggt gaaccacaag ggatcagaga
     1081 aaccacttga gcaaacattc gcaaacatgg tgtcctcctt gggaagtggg atgatcagat
     1141 atattgcctt tgacttccat aaggagtgta aaaatatgag atgggatcga ttgagtattt
     1201 tattggatca agtagcagaa atgcaggatg aattaagtta ttttttagta gactctgccg
     1261 gcaaggtggt gacaaaccag gacggagttt tccgcagcaa ctgcatggat tgtctagaca
     1321 gaacgaatgt gatacagagt ttattagctc gacgttccct tcaggcacag ctgcagaggc
     1381 taggagtttt gcatgtggga cagaagcttg aagagcaaga tgaatttgag aagacttaca
     1441 aaaatgcctg ggccgataat gctaatgctt gtgccaaaca gtatgcggga accggtgcct
     1501 tgaagactga ctttacaaga actggaaaaa gaactcagtt gggactatta atggatggct
     1561 tcaactcatt attacggtat tacaagaaca acttttctga tggatttaga caagattcca
     1621 tagacttatt tcttggaaat tattcagtgg atgaattaga atctcatagc cctttaagtg
     1681 ttccaaggga ctggaaattc ctggcgttgc ctatcatcat ggttgttgcc ttttcaatgt
     1741 gcatcatctg tttgcttatg gctggtgaca cttggacaga aacactggca tatgtcctct
     1801 tctggggagt tgcaagcatt ggaacatttt ttattattct ttacaatggc aaagattttg
     1861 ttgatgctcc cagactggtc cagaaagaaa agatagactg aatttgtgtt tgtggaaagc
     1921 ggcttggctt ggaagactcc atgatgcaga actggagtct ttactgacct gctttccacg
     1981 tgagcccaag gtcttcttaa agcctttatc caaaagtacc tcctgtggtt ttgggctggg
     2041 tgatgtctta gaattgatct gatattaccg ctttgatgat ctcgttaccg ttgggatggt
     2101 tatatttatg atttgaagct atagtataat taaaatgtag cttaaattca gctcacagaa
     2161 cagaagaaat ctattcactg tcaagtcaga attcacagat aagtaatata ttttaaaaaa
     2221 tttaacttct ttcacattat ttaaaacaaa ttatttttct agctctgttt cattattgcc
     2281 attgtaaagc agtcactgtt agcaagcata ttttccatgt atctttgggc ttttcttaag
     2341 agtttaataa tatgcaaatt ctactatgct tatagagtgt ctcttaaagg tcaagcagtt
     2401 tgctaaattg taatttcaca attgtaagga agaggagaat gggatctaga gtcatttgaa
     2461 gtttctagta gctgcctctt ggcttcagcc agcttgagct gctgtagtgc ccgagcagct
     2521 tatatgtcat gaaaagttca gatatagttt atctttttta aaaggtataa atgaaatcaa
     2581 agaatgtaaa gtctgtctct tatgctaaag gtccaaagtc gcctcttttt aagaactgct
     2641 ccattgtggg aaatacacta gactggccag ctgcttcctc tcctagcttg taggattttt
     2701 tattattggt tcctcattga gacatgcagg cctcagagaa ggtcttgtac tagatttgat
     2761 tctcacttgg ggtagcagtt gcttttccat gcatggcact ctggctctat caccctgtca
     2821 aggtttatgt tggctgtcaa taagtgtctg aaatttagtg cttccaggta gccatagttc
     2881 tccaaatcaa ggaccaactt taatttatgc ataaaacaga ctgaaaacta cgcttcagaa
     2941 gctgtgcata gttgtaagac aggttctaga ttaaaattgt aattatgagg tttagatatt
     3001 agaacagtat ataaggtagt atcattgcac tatcttaaaa caattcagtt aaacactgct
     3061 atggtacatc agtgttttgc tttaaaatat gtaggttttc taatagtacc ttatgttatc
     3121 cataaatatt tctaaaagcg cctttaacat tacctttttt caacattatc ttttataaac
     3181 aataagggtt attgctttta ggaactctgg ggaaattctg tgtagcttaa aatcagtcat
     3241 taaaactcac aatagagtaa gtaaatagag ccacctgttc tgacatgtaa ataagcatag
     3301 tttgttccaa agatggaaca ttgaagccca gctgtgtcta ctgtaatata ttactcaaaa
     3361 gctactgggc gttttacagt aagcacttca tgtcaacagc tacagcaaac accaaatctg
     3421 aaccactagt gtggctgctt cgcaaggact aatgtcagag ggttaggctg caagtgatca
     3481 gtgtttgaca gccctgcagt gatcttcttt aactcattta ctgtacatcc atatgcaaaa
     3541 ataaaatgcc atattctttt ctaaaaaaaa aaaaaaaaaa
//
