LOCUS       NM_028456               1482 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus RWD domain containing 3 (Rwdd3), transcript variant 2,
            mRNA.
ACCESSION   NM_028456
VERSION     NM_028456.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1482)
  AUTHORS   Loo,L., Simon,J.M., Xing,L., McCoy,E.S., Niehaus,J.K., Guo,J.,
            Anton,E.S. and Zylka,M.J.
  TITLE     Single-cell transcriptomic analysis of mouse neocortical
            development
  JOURNAL   Nat Commun 10 (1), 134 (2019)
   PUBMED   30635555
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1482)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   5  (bases 1 to 1482)
  AUTHORS   Shan,B., Gerez,J., Haedo,M., Fuertes,M., Theodoropoulou,M.,
            Buchfelder,M., Losa,M., Stalla,G.K., Arzt,E. and Renner,U.
  TITLE     RSUME is implicated in HIF-1-induced VEGF-A production in pituitary
            tumour cells
  JOURNAL   Endocr Relat Cancer 19 (1), 13-27 (2012)
   PUBMED   22009797
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1482)
  AUTHORS   Kawaguchi,A., Ikawa,T., Kasukawa,T., Ueda,H.R., Kurimoto,K.,
            Saitou,M. and Matsuzaki,F.
  TITLE     Single-cell gene profiling defines differential progenitor
            subclasses in mammalian neurogenesis
  JOURNAL   Development 135 (18), 3113-3124 (2008)
   PUBMED   18725516
REFERENCE   7  (bases 1 to 1482)
  AUTHORS   Carbia-Nagashima,A., Gerez,J., Perez-Castro,C., Paez-Pereda,M.,
            Silberstein,S., Stalla,G.K., Holsboer,F. and Arzt,E.
  TITLE     RSUME, a small RWD-containing protein, enhances SUMO conjugation
            and stabilizes HIF-1alpha during hypoxia
  JOURNAL   Cell 131 (2), 309-323 (2007)
   PUBMED   17956732
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY744183.1, AK134288.1 and BC037515.1.
            
            On Apr 14, 2010 this sequence version replaced NM_028456.2.
            
            Transcript Variant: This variant (2) lacks an in-frame segment in
            the 5' coding region, compared to variant 1, resulting in an
            isoform (2) that is shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134288.1, AK014130.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY744183.1         2-12
            12-1477             AK134288.1         3-1468
            1478-1482           BC037515.1         1206-1210
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 52.94 cM"
     gene            1..1482
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /note="RWD domain containing 3"
                     /db_xref="GeneID:66568"
                     /db_xref="MGI:MGI:1920420"
     exon            1..104
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..823
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /note="isoform 2 is encoded by transcript variant 2; RWD
                     domain-containing protein 3; RWD domain-containing
                     sumoylation enhancer"
                     /codon_start=1
                     /product="RWD domain-containing protein 3 isoform 2"
                     /protein_id="NP_082732.2"
                     /db_xref="CCDS:CCDS17798.1"
                     /db_xref="GeneID:66568"
                     /db_xref="MGI:MGI:1920420"
                     /translation="MVEEVRQELSALAAIFCGPNEWEMLSCSETDGAVFRIHTTAEGL
                     VGEDVPLELAFHLPVGYPLCLPGISVTSEHLTRAQCVTAKEKLLGEARKLVSEPMVHE
                     LVLWIQQNLRLVLSQPETASSHEKCTLPESATGDDGPWMTLLRLDHMRARTKYVKAVE
                     KWASELRLTGRLMFMGKLILILLQGDRSNIKEYLILQKTSKVDVDSSGKKCKEKMISV
                     LSETKVQTEHKRFLAFEVKEYSTLEELQKEFGAAGLGELFSECVLGLVK"
     exon            105..592
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /inference="alignment:Splign:2.1.0"
     exon            593..708
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /inference="alignment:Splign:2.1.0"
     exon            709..824
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /inference="alignment:Splign:2.1.0"
     exon            825..1482
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1019..1024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /note="hexamer: ATTAAA"
     polyA_site      1040
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /note="major polyA site"
     regulatory      1457..1462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
                     /note="hexamer: ATTAAA"
     polyA_site      1482
                     /gene="Rwdd3"
                     /gene_synonym="2510027J23Rik; 3110037C01Rik; Rsume; X2CR1"
ORIGIN      
        1 gggttctggt cgcacggata tggtggagga ggtgcggcag gagctctcgg cgctggccgc
       61 gatcttctgc gggcccaacg agtgggagat gttgagctgt tcagagacag atggggctgt
      121 gtttagaatt cacacaacag ctgaaggact tgtgggtgag gatgtgccct tagagttggc
      181 gttccattta ccagttggtt accctttgtg tctacccggc atctcggtta cctcggaaca
      241 tctgaccagg gcccagtgtg ttaccgcgaa ggagaagtta cttggagaag ccaggaagct
      301 cgtgtcagaa ccgatggttc acgagctggt tctttggatc cagcagaatc tcagacttgt
      361 cctcagccag ccggagaccg ccagcagcca tgagaagtgc actttgccag aaagtgcgac
      421 gggggacgat ggaccgtgga tgactctttt gcgtttagat cacatgagag cgaggactaa
      481 atatgtcaaa gccgtggaga agtgggcttc agagttaagg ctgacaggaa gactgatgtt
      541 catgggtaaa ttaatactga ttttactaca aggagacaga agcaacatca aggagtacct
      601 gattcttcag aaaacctcca aagtagatgt ggactcaagt ggaaagaaat gcaaagagaa
      661 aatgattagt gtactgtctg aaacaaaagt acaaacggaa cacaaaaggt ttctggcgtt
      721 tgaagtcaaa gagtattcaa cactggagga gttacaaaag gaattcggag ctgcaggact
      781 tggggagctc ttctcagagt gtgtactggg gctggtgaaa tgaggaagaa aagcgagtcc
      841 tgttttatgg aatattaaac atgaaaagat cccatatatt ctaacgtttg ccagaaaaga
      901 ctaggttcaa tagacggatt gtctggagga gagagaaacc caccacagta aaggaactgt
      961 tgtttttata gagacaattt gtttagaact ctgcaatttt caggctaatt ttctcgtaat
     1021 taaatgattt cttaaagtga tttgttttca tgtttacttt ctcatgttta tgttttttgc
     1081 ctttaatcgt gccttcctcc ccactctgct tattatgttt taagttggat gttgagaaaa
     1141 atctaacact catttcttgg tgcggttgca taaatcgttg gattaaatac aaaaccaaga
     1201 agttttacag aacacagttg ctttctggag agctggaaga atacacagtg agttcaaatc
     1261 gaaattcata tgtgaaaaaa ataatttctg gttttacaag gaaaatgtgt ccaacaaagt
     1321 gaaccctttt tatttttttt ggtggaaaaa caaaccaaac tgtagctcta caattttcat
     1381 tgtgaaatct ctcaaataat ggtaaaatgt actaactatg tccaagggga gataaactcg
     1441 cagcataatt atttatatta aaatttatga tgaaatgtaa aa
//
