LOCUS       NM_001145969            1239 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus RWD domain containing 2A (Rwdd2a), transcript variant
            3, mRNA.
ACCESSION   NM_001145969
VERSION     NM_001145969.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ149958.1, CN836837.1, AK006533.1 and AI848608.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CJ149958.1, DV047023.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                CJ149958.1         1-27
            28-553              CN836837.1         31-556
            554-1220            AK006533.1         648-1314
            1221-1239           AI848608.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 46.58 cM"
     gene            1..1239
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /note="RWD domain containing 2A"
                     /db_xref="GeneID:69519"
                     /db_xref="MGI:MGI:1916769"
     exon            1..70
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /inference="alignment:Splign:2.1.0"
     exon            71..297
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..975
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /note="RWD domain containing 2"
                     /codon_start=1
                     /product="RWD domain-containing protein 2A"
                     /protein_id="NP_001139441.1"
                     /db_xref="CCDS:CCDS23382.1"
                     /db_xref="GeneID:69519"
                     /db_xref="MGI:MGI:1916769"
                     /translation="MSGSVKESLQLQLLEMEMLFSMFPNQGEVKLEDVNALTNIKRYL
                     EGTREALPPNIEFVITLQIEEPKVTIDLQVTMPHNYPYVALQLFGRSPELDRQQQLLL
                     NQALSAYLGTFDPGELCVCAAIQWLQDNSASYFLTRKLSDEPSVQAKPVKNTFLRMWI
                     YSHHIYQQDLRKKILEVGKRLDVTGFCMTGKPGIICVEGFKNHCEEFWHTIRYPNWKH
                     ISCKHAESVETEGDGEDLRLFHSFEELLLEAHGDYGLRNDYHMNLGQFLEFLRKHKSE
                     HVFQILFGIESKSSES"
     exon            298..1223
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1200..1205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /note="hexamer: AATAAA"
     polyA_site      1223
                     /gene="Rwdd2a"
                     /gene_synonym="1700030C20Rik; Rwdd2"
                     /note="major polyA site"
ORIGIN      
        1 tcgcgcccgc ggaactgcgg ctgcggttcg gagcgggttc ggggctgctt ggcttgagtg
       61 atctgaaagg gtttccaagc cggctgattt ggagacatgt cagggtcagt aaaagaaagc
      121 cttcagcttc agctgctgga gatggaaatg ctgttttcta tgtttcctaa tcaaggagaa
      181 gtaaaactcg aagatgtaaa cgccctaaca aacataaaga ggtatctgga aggcaccagg
      241 gaggcgctcc cacccaacat cgaatttgtc atcacgctcc aaattgagga gcccaaggtg
      301 accattgatt tgcaagtaac catgcctcac aactacccat acgtggctct gcagctgttt
      361 ggacggtcac ctgagcttga cagacaacag cagctgctgc ttaaccaagc tctcagtgct
      421 tatctcggga ctttcgaccc aggtgagttg tgtgtctgtg cagccatcca gtggctgcag
      481 gacaacagcg catcctactt cctcaccaga aagctgtcgg acgagccatc cgtacaggcg
      541 aagccagtca agaacacatt cctccgcatg tggatctata gccaccacat atatcagcag
      601 gacctgagga aaaagatctt ggaagtggga aaaaggttag atgtgactgg attttgcatg
      661 acaggaaagc ctggtataat ctgtgtggag ggcttcaaga atcactgtga ggaattctgg
      721 cacacaatca gataccccaa ttggaaacac atttcctgca agcatgcaga aagtgtggag
      781 acagaggggg acggtgaaga cctgcgcctc ttccattcgt ttgaggaatt actccttgag
      841 gcccatggtg actatggatt gcggaatgat tatcacatga acctgggcca attcctggag
      901 tttctgagaa aacacaagag tgagcatgtt tttcagatat tatttggtat tgaaagcaaa
      961 agctcagagt cctagaaccc tattaagatg tctacgcttg taaccgcatt aagtatttat
     1021 gttactaaga ccaaaataaa aaggctaatg acaatataga attctgggtg aaaaacctag
     1081 ctctagaatt tcctgactgc tgatgctaat aggcccttta actttgtacc agtgtaactg
     1141 tgatgctgtt tccaggttgc tgtgtaaagt cacacttgat taccatttca ttataaatca
     1201 ataaaatgat atatttaacc agaaaaaaaa aaaaaaaaa
//
