LOCUS       NM_001271630            5300 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus runt related transcription factor 2 (Runx2),
            transcript variant 5, mRNA.
ACCESSION   NM_001271630
VERSION     NM_001271630.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Takebe,H., Sato,H., Mizoguchi,T. and Hosoya,A.
  TITLE     Localization of alpha-smooth muscle actin in osteoblast
            differentiation during periodontal development
  JOURNAL   Cell Tissue Res 399 (1), 119-127 (2025)
   PUBMED   39579220
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Hudacova,E., Abaffy,P., Kaplan,M.M., Krausova,M., Kubista,M. and
            Machon,O.
  TITLE     Single-cell transcriptomic resolution of osteogenesis during
            craniofacial morphogenesis
  JOURNAL   Bone 190, 117297 (2025)
   PUBMED   39461490
REFERENCE   3  (bases 1 to 5300)
  AUTHORS   Zerad,L., Gacem,N., Gayda,F., Day,L., Sinigaglia,K., Richard,L.,
            Parisot,M., Cagnard,N., Mathis,S., Bole-Feysot,C., O'Connell,M.A.,
            Pingault,V., Dambroise,E., Keegan,L.P., Vallat,J.M. and
            Bondurand,N.
  TITLE     Overexpression of Egr1 Transcription Regulator Contributes to
            Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1
            Knockout Mice
  JOURNAL   Cells 13 (23), 1952 (2024)
   PUBMED   39682701
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5300)
  AUTHORS   Gilbert,L., He,X., Farmer,P., Rubin,J., Drissi,H., van Wijnen,A.J.,
            Lian,J.B., Stein,G.S. and Nanes,M.S.
  TITLE     Expression of the osteoblast differentiation factor RUNX2
            (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis
            factor-alpha
  JOURNAL   J Biol Chem 277 (4), 2695-2701 (2002)
   PUBMED   11723115
REFERENCE   5  (bases 1 to 5300)
  AUTHORS   Sudhakar,S., Katz,M.S. and Elango,N.
  TITLE     Analysis of type-I and type-II RUNX2 protein expression in
            osteoblasts
  JOURNAL   Biochem Biophys Res Commun 286 (1), 74-79 (2001)
   PUBMED   11485310
REFERENCE   6  (bases 1 to 5300)
  AUTHORS   Fujiwara,M., Tagashira,S., Harada,H., Ogawa,S., Katsumata,T.,
            Nakatsuka,M., Komori,T. and Takada,H.
  TITLE     Isolation and characterization of the distal promoter region of
            mouse Cbfa1
  JOURNAL   Biochim Biophys Acta 1446 (3), 265-272 (1999)
   PUBMED   10524201
REFERENCE   7  (bases 1 to 5300)
  AUTHORS   Thirunavukkarasu,K., Mahajan,M., McLarren,K.W., Stifani,S. and
            Karsenty,G.
  TITLE     Two domains unique to osteoblast-specific transcription factor
            Osf2/Cbfa1 contribute to its transactivation function and its
            inability to heterodimerize with Cbfbeta
  JOURNAL   Mol Cell Biol 18 (7), 4197-4208 (1998)
   PUBMED   9632804
REFERENCE   8  (bases 1 to 5300)
  AUTHORS   Stewart,M., Terry,A., Hu,M., O'Hara,M., Blyth,K., Baxter,E.,
            Cameron,E., Onions,D.E. and Neil,J.C.
  TITLE     Proviral insertions induce the expression of bone-specific isoforms
            of PEBP2alphaA (CBFA1): evidence for a new myc collaborating
            oncogene
  JOURNAL   Proc Natl Acad Sci U S A 94 (16), 8646-8651 (1997)
   PUBMED   9238031
REFERENCE   9  (bases 1 to 5300)
  AUTHORS   Levanon,D., Negreanu,V., Bernstein,Y., Bar-Am,I., Avivi,L. and
            Groner,Y.
  TITLE     AML1, AML2, and AML3, the human members of the runt domain
            gene-family: cDNA structure, expression, and chromosomal
            localization
  JOURNAL   Genomics 23 (2), 425-432 (1994)
   PUBMED   7835892
REFERENCE   10 (bases 1 to 5300)
  AUTHORS   Sohn,K.Y., Maity,S.N. and de Crombrugghe,B.
  TITLE     Studies on the structure of the mouse CBF-A gene and properties of
            a truncated CBF-A isoform generated from an alternatively spliced
            RNA
  JOURNAL   Gene 139 (2), 147-153 (1994)
   PUBMED   7509302
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165141.4.
            
            On Dec 29, 2022 this sequence version replaced NM_001271630.1.
            
            Summary: This gene encodes a member of the runt domain-containing
            family of transcription factors. This protein is essential for
            osteoblastic differentiation and skeletal morphogenesis and acts as
            a scaffold for nucleic acids and regulatory factors involved in
            skeletal gene expression. The protein can bind DNA both as a
            monomer or, with more affinity, as a subunit of a heterodimeric
            complex. Transcript variants that encode different protein isoforms
            result from the use of alternate promoters as well as alternate
            splicing. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (5) represents use of a proximal
            promoter. It lacks two alternate exons including the 5' UTR and a
            portion of the 5' coding region, initiates translation in an
            alternate 5' segment, and lacks an internal in-frame coding exon,
            compared to variant 1. The encoded isoform (3) has a distinct
            N-terminus and is shorter than isoform 1.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.5303807.1,
                                           SRR13422598.849136.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-427               AC165141.4         162041-162467       c
            428-584             AC165141.4         151553-151709       c
            585-689             AC165141.4         144000-144104       c
            690-851             AC165141.4         66473-66634         c
            852-917             AC165141.4         36836-36901         c
            918-5300            AC165141.4         30809-35191         c
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 21.33 cM"
     gene            1..5300
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="runt related transcription factor 2"
                     /db_xref="GeneID:12393"
                     /db_xref="MGI:MGI:99829"
     exon            1..427
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1396
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="isoform 3 is encoded by transcript variant 5; AKV
                     core binding factor; PEA2-alpha A; acute myeloid leukemia
                     3 protein; SL3-3 enhancer factor 1 alpha A subunit;
                     osteoblast-specific transcription factor 2; polyomavirus
                     enhancer-binding protein 2 alpha A subunit; core binding
                     factor alpha 1; PEBP2 alpha A; transcription factor
                     Cbfa1/Osf2; runt domain, alpha subunit 1"
                     /codon_start=1
                     /product="runt-related transcription factor 2 isoform 3"
                     /protein_id="NP_001258559.1"
                     /db_xref="GeneID:12393"
                     /db_xref="MGI:MGI:99829"
                     /translation="MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQ
                     QQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADH
                     PAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAE
                     LRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPR
                     EPRNPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISD
                     DDTATSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFT
                     YTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSASYQFP
                     MVPGGDRSPSRMVPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDE
                     SVWRPY"
     exon            428..584
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            585..689
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            690..851
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            852..917
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            918..5300
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5280..5285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="hexamer: AATAAA"
     polyA_site      5300
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="major polyA site"
ORIGIN      
        1 acttcgctaa cttgtggctg ttgtgatgcg tattcctgta gatccgagca ccagccggcg
       61 cttcagcccc ccctccagca gcctgcagcc cggcaagatg agcgacgtga gcccggtggt
      121 ggctgcgcag cagcagcaac agcagcagca gcagcagcaa cagcagcagc aacaacagca
      181 acagcaacaa cagcagcagc agcagcagca gcaggaggcg gccgcagcag cagcggcggc
      241 agcggcggcg gcagcagcgg cggcggccgc agtgccccga ttgaggccgc cgcacgacaa
      301 ccgcaccatg gtggagatca tcgcggacca cccggccgaa ctggtccgca ccgacagtcc
      361 caacttcctg tgctccgtgc tgccctcgca ctggcggtgc aacaagaccc tgcccgtggc
      421 cttcaaggtt gtagccctcg gagaggtacc agatgggact gtggttaccg tcatggccgg
      481 gaatgatgag aactactccg ccgagctccg aaatgcctcc gctgttatga aaaaccaagt
      541 agccaggttc aacgatctga gatttgtggg ccggagcgga cgaggcaaga gtttcacctt
      601 gaccataaca gtcttcacaa atcctcccca agtggccact taccacagag ctattaaagt
      661 gacagtggac ggtccccggg aaccaagaaa tcccaggcag gcacagtctt ccccaccgtg
      721 gtcctatgac cagtcttacc cctcctatct gagccagatg acatccccat ccatccactc
      781 caccacgccg ctgtcttcca cacggggcac cgggctacct gccatcactg acgtgcccag
      841 gcgtatttca gatgatgaca ctgccacctc tgacttctgc ctctggcctt cctctctcag
      901 taagaagagc caggcaggtg cttcagaact gggccctttt tcagacccca ggcagttccc
      961 aagcatttca tccctcactg agagccgctt ctccaaccca cgaatgcact acccagccac
     1021 ctttacctac accccgccag tcacgtcagg catgtccctc ggcatgtccg ccaccactca
     1081 ctaccacacg tacctgccac caccctaccc cggctcttcc caaagccaga gtggaccctt
     1141 ccagaccagc agcactccat atctctacta tggtacttcg tcagcatcct atcagttccc
     1201 aatggtaccc gggggagacc ggtctccttc caggatggtc ccaccatgca ccaccacctc
     1261 gaatggcagc acgctattaa atccaaattt gcctaaccag aatgatggtg ttgacgctga
     1321 cggaagccac agcagttccc caactgtttt gaattctagc ggcagaatgg atgagtctgt
     1381 ttggcggcca tattgaaatt cgtcaaccat ggcccagtgg catgggggcc acatcccgca
     1441 tgtgttaata tatacatata taaagagagt gcctatatat gtatattgat tagctaacta
     1501 gaagatttct cattcaatcc ctagtcatga tcttgcaacc ctaagagggt gggggcagtc
     1561 ataactgggt ttcatattgt ttactattta agatgtcccc tttaccaagg aacaaaccgt
     1621 caaaggtgtt gtctggtctg ttttcataag tgacctgttc ccacgccggt tcagagaggt
     1681 ggactctggg tctgggagga aggagagaca cttcctctct gtgctttgaa accacagcct
     1741 ctgctgtgtg gcagccggta cactctgcag acccgcttac agagtcagat gtggtgcact
     1801 cagaaaggga caagaggcag agtggctgct tctgtccgct gccgtccact ctgccgtcca
     1861 cctgttccaa agttttcctt cagacttgct gcaggtactc atttgaactt ttgagttcac
     1921 tttttttttt tcctattcta agaaagtgac ttcaaaaata ctgatcagga cagataattt
     1981 tattttacct tttatatttt ctcacttccc ccatttaacc aaaaagaaat cccgttcccc
     2041 ctcccccgtt ccttctgctt ctccctttat gcaaactgaa aatggcaatg ccttattatt
     2101 atagccataa tggtatagtg tttgagttgg ctgtgtgtta tgtgtttttt tctttttttt
     2161 tcttttttaa attatgaata tgtgtaaaat ctgaagtaac ttgctaacgt gaatggtcat
     2221 ataactttaa agatatattt ataattattt aatgacattt ggacatttgg aacatttctt
     2281 agtgtaatga tatgttgact tcggtctcta aaagtgtgct tcttcttcaa taccaagttt
     2341 cttcagtggg ctagagccat atcggaaata ttgctaagca atctcaattc cttcaggcat
     2401 aatgtgattt tttttttttt ttgaagataa ctcccatctc caaatagttt agatgtagtt
     2461 tgttttcacg atgtatgaag gagatgctct gtttctttct ttcaggcatt tgattgcctc
     2521 tgacacagct ttgcctttta aagcaataat tagggattaa aataacaaaa acaaaacaaa
     2581 agccacctat agccctttaa cacttaacgt ggccccttta ctagcatgaa atgctggaga
     2641 catgtggttt cctaatttct ccattttggg ggtggtggga ggggggaggg tggccattat
     2701 gactcttatc atattaaaaa gccaagcaca agtgattggt tgaactgcag aaaagtgttc
     2761 tgtggtctct gagttgagca aaactctaaa ttgcaggctt cgtggttgag ggcctagtca
     2821 gctgaaagcc acgcgtgtgg taaaggctca ggcatggctt ggagaaccta ggaacacatt
     2881 aggagcctgc acctaccagc ctcaccatac agccattcag gggaacccaa aaagtgcctt
     2941 acccaaggag ggcccccagc agctttccag gaagtcgaat gaagtcgctg tcctcgggga
     3001 actggtcagc tgaagtagca accggtagct gatgtcagta gacaacagaa cctgtgggga
     3061 cctccaggaa acctttgaca ttggaggctt tcattaggca gggccaacaa gagcagggaa
     3121 ggccatgtac ccattggtat ctgccattgt gtgtgagttt gagatccagc cctccttgga
     3181 ggatgtactg tgatcattcc tggtacctta gggccaatcc ctaagtgtgg cttcctaatc
     3241 caggccaggg atcattcagt gataccacca ggccaatccc agcattcctc ctgcacaaag
     3301 tgtttgtgtg tggggggata gattggggtg gggccacctt catttgaatc ctgagtcatt
     3361 ctaagagttc tgcaagcttt tgccttcagc accctatacc ccctcgctct ctgttccttc
     3421 tcaggttgac ctttgtccca atgcgggaca gtccagaggc agatggggac ctatgtgtgc
     3481 ctccaacctg cgttttcctc agaattcatc ctgactccct ctgacacaga ttgagggggg
     3541 ggggaagaaa cccaacccgc accaaagcaa aacactttgc cttctaaagg ctgtgcacca
     3601 agtagacgca gatggtcagc ccacctttgt gtttccttaa gatggaaatt gtaactgatg
     3661 ctatttattg tttgtgtgtg gtagcttgaa gcacaccacg gtccatgtgt ttgtctgata
     3721 cctatttcag cagcatttac aaaagccagt gtctggttac acttttcagt tttcattaat
     3781 caacatgaaa atgttaccat tggtgccagc tgcaccctat ttaatttttt taagggcact
     3841 atatttgtac atttcgtttt taatgttaaa gggcttctta aagtttacag tacagttatc
     3901 aagggaatag aggggatgca ttagtgccta aatgttattc tagtggctgc aggcagcaac
     3961 ccagaagcag ttttgaaaac aggttgtttc cctctgtcct cccttatttg ggaaaattca
     4021 agtgctttct tcacctttca ggcacctcac ggtgactccc gttacttaga gcagtctgtc
     4081 gtcgtcttct tcttcttcct cttcctcttc ctcttcctct tcctcttcct cttcctcttc
     4141 ctcttcctct tcctcttctt cttcttcttc ttcttcttct tcttcttctt cttcctccta
     4201 gggtttaaaa tcccccttcc tcttccctta tctcttaaac tcttcatttg cactgggtca
     4261 cacgtatgat ttgtggtttt aagaccaaag caatgtctta ttactcttct ggagccgttt
     4321 atgtgtacta accaaccctt ccctccactt ccctgggttt agatgcacac ggttctcaaa
     4381 ggagcacaaa catggccaga ttcacagtgg gacccacaca gccatgttaa aaaaaaaaac
     4441 aaaaaaaaac ctcttcactt gtctgagaat tttaacctgg gccccaattg ctaacgggca
     4501 ccatggcttg ggtttcaggt tagggaacgt tgcccagtga gtaacagaaa gacttaactg
     4561 atttaattag tttgccctca tccttcactc caagacccta agaaaccgat cagagaaaca
     4621 tctccacacc attagaggtt gagaagggag gagccatgtg ggggttccct ccgctcggag
     4681 gaaaggcact gactgaccta gttagagtgg tagcagaagc acccatggta tctgagagag
     4741 caataaacaa taaaagatga tcctcccaag cttagaattt gttgttctta aagaagacga
     4801 tgcatctaaa acaaagaatt tttttttcta tgtgggaact ttcttcgttg tctgtttact
     4861 tcatgtttac aaataattgt ttgcttttta atgcagtact ttaaaaaata tatcagccaa
     4921 aaccataact tacagtaatt tttttaggta ttctgaataa aattccatgt cttttgatat
     4981 gtctactgtt cttaggtttc tgtggaacaa caacaacaac aacaacaaaa ttgtagcatt
     5041 ttgtgtaaat acagctttcg ttcttatttt ttatttttct gattgctatt gcccaagatt
     5101 tgctttctat gcacgtattg tacaaattgt gctttgtgcc acaggtcatg atcgtggatg
     5161 agtttactct gaacttcaaa gggactattt gtattgtatg ttgcaactgt aaattgaatt
     5221 atttggcatt tccccctctc atgattgtaa tattaatttg aagtttgaat ttaattttca
     5281 ataaaaaggc tttttttttc
//
