LOCUS       NM_001271627            5896 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus runt related transcription factor 2 (Runx2),
            transcript variant 4, mRNA.
ACCESSION   NM_001271627
VERSION     NM_001271627.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Takebe,H., Sato,H., Mizoguchi,T. and Hosoya,A.
  TITLE     Localization of alpha-smooth muscle actin in osteoblast
            differentiation during periodontal development
  JOURNAL   Cell Tissue Res 399 (1), 119-127 (2025)
   PUBMED   39579220
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Hudacova,E., Abaffy,P., Kaplan,M.M., Krausova,M., Kubista,M. and
            Machon,O.
  TITLE     Single-cell transcriptomic resolution of osteogenesis during
            craniofacial morphogenesis
  JOURNAL   Bone 190, 117297 (2025)
   PUBMED   39461490
REFERENCE   3  (bases 1 to 5896)
  AUTHORS   Zerad,L., Gacem,N., Gayda,F., Day,L., Sinigaglia,K., Richard,L.,
            Parisot,M., Cagnard,N., Mathis,S., Bole-Feysot,C., O'Connell,M.A.,
            Pingault,V., Dambroise,E., Keegan,L.P., Vallat,J.M. and
            Bondurand,N.
  TITLE     Overexpression of Egr1 Transcription Regulator Contributes to
            Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1
            Knockout Mice
  JOURNAL   Cells 13 (23), 1952 (2024)
   PUBMED   39682701
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5896)
  AUTHORS   Gilbert,L., He,X., Farmer,P., Rubin,J., Drissi,H., van Wijnen,A.J.,
            Lian,J.B., Stein,G.S. and Nanes,M.S.
  TITLE     Expression of the osteoblast differentiation factor RUNX2
            (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis
            factor-alpha
  JOURNAL   J Biol Chem 277 (4), 2695-2701 (2002)
   PUBMED   11723115
REFERENCE   5  (bases 1 to 5896)
  AUTHORS   Sudhakar,S., Katz,M.S. and Elango,N.
  TITLE     Analysis of type-I and type-II RUNX2 protein expression in
            osteoblasts
  JOURNAL   Biochem Biophys Res Commun 286 (1), 74-79 (2001)
   PUBMED   11485310
REFERENCE   6  (bases 1 to 5896)
  AUTHORS   Fujiwara,M., Tagashira,S., Harada,H., Ogawa,S., Katsumata,T.,
            Nakatsuka,M., Komori,T. and Takada,H.
  TITLE     Isolation and characterization of the distal promoter region of
            mouse Cbfa1
  JOURNAL   Biochim Biophys Acta 1446 (3), 265-272 (1999)
   PUBMED   10524201
REFERENCE   7  (bases 1 to 5896)
  AUTHORS   Thirunavukkarasu,K., Mahajan,M., McLarren,K.W., Stifani,S. and
            Karsenty,G.
  TITLE     Two domains unique to osteoblast-specific transcription factor
            Osf2/Cbfa1 contribute to its transactivation function and its
            inability to heterodimerize with Cbfbeta
  JOURNAL   Mol Cell Biol 18 (7), 4197-4208 (1998)
   PUBMED   9632804
REFERENCE   8  (bases 1 to 5896)
  AUTHORS   Stewart,M., Terry,A., Hu,M., O'Hara,M., Blyth,K., Baxter,E.,
            Cameron,E., Onions,D.E. and Neil,J.C.
  TITLE     Proviral insertions induce the expression of bone-specific isoforms
            of PEBP2alphaA (CBFA1): evidence for a new myc collaborating
            oncogene
  JOURNAL   Proc Natl Acad Sci U S A 94 (16), 8646-8651 (1997)
   PUBMED   9238031
REFERENCE   9  (bases 1 to 5896)
  AUTHORS   Levanon,D., Negreanu,V., Bernstein,Y., Bar-Am,I., Avivi,L. and
            Groner,Y.
  TITLE     AML1, AML2, and AML3, the human members of the runt domain
            gene-family: cDNA structure, expression, and chromosomal
            localization
  JOURNAL   Genomics 23 (2), 425-432 (1994)
   PUBMED   7835892
REFERENCE   10 (bases 1 to 5896)
  AUTHORS   Sohn,K.Y., Maity,S.N. and de Crombrugghe,B.
  TITLE     Studies on the structure of the mouse CBF-A gene and properties of
            a truncated CBF-A isoform generated from an alternatively spliced
            RNA
  JOURNAL   Gene 139 (2), 147-153 (1994)
   PUBMED   7509302
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672242.12 and AC165141.4.
            
            On Dec 29, 2022 this sequence version replaced NM_001271627.1.
            
            Summary: This gene encodes a member of the runt domain-containing
            family of transcription factors. This protein is essential for
            osteoblastic differentiation and skeletal morphogenesis and acts as
            a scaffold for nucleic acids and regulatory factors involved in
            skeletal gene expression. The protein can bind DNA both as a
            monomer or, with more affinity, as a subunit of a heterodimeric
            complex. Transcript variants that encode different protein isoforms
            result from the use of alternate promoters as well as alternate
            splicing. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (4) represents use of a distal
            promoter. It has an additional segment in 5' UTR and encodes the
            same isoform 1, compared to variant 1. This variant is reported in
            PMID: 9238031.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF005936.1, SRR13948564.5348785.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:11723115)
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-463               AL672242.12        22725-23187
            464-849             AC165141.4         162041-162426       c
            850-1006            AC165141.4         151553-151709       c
            1007-1111           AC165141.4         144000-144104       c
            1112-1285           AC165141.4         85327-85500         c
            1286-1447           AC165141.4         66473-66634         c
            1448-1513           AC165141.4         36836-36901         c
            1514-5896           AC165141.4         30809-35191         c
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 21.33 cM"
     gene            1..5896
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="runt related transcription factor 2"
                     /db_xref="GeneID:12393"
                     /db_xref="MGI:MGI:99829"
     exon            1..463
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             406..1992
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="isoform 1 is encoded by transcript variant 4; AKV
                     core binding factor; PEA2-alpha A; acute myeloid leukemia
                     3 protein; SL3-3 enhancer factor 1 alpha A subunit;
                     osteoblast-specific transcription factor 2; polyomavirus
                     enhancer-binding protein 2 alpha A subunit; core binding
                     factor alpha 1; PEBP2 alpha A; transcription factor
                     Cbfa1/Osf2; runt domain, alpha subunit 1"
                     /codon_start=1
                     /product="runt-related transcription factor 2 isoform 1"
                     /protein_id="NP_001258556.1"
                     /db_xref="CCDS:CCDS37624.2"
                     /db_xref="GeneID:12393"
                     /db_xref="MGI:MGI:99829"
                     /translation="MASNSLFSAVTPCQQSFFWDPSTSRRFSPPSSSLQPGKMSDVSP
                     VVAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAAAAAAAAAAAVPRLRP
                     PHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGEVPDGTV
                     VTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVA
                     TYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPS
                     LNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGT
                     GLPAITDVPRRISDDDTATSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTES
                     RFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTP
                     YLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNPNLPNQNDGVDADGSHS
                     SSPTVLNSSGRMDESVWRPY"
     exon            464..849
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1006
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1111
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1285
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1447
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1513
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..5896
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5876..5881
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="hexamer: AATAAA"
     polyA_site      5896
                     /gene="Runx2"
                     /gene_synonym="AML3; Cbf; CBF-alpha-1; Cbfa-1; Cbfa1; LS3;
                     Osf2; Pebp2a1; PEBP2aA; Pebpa2a"
                     /note="major polyA site"
ORIGIN      
        1 gtgaatgctt cattcgcctc acaaacaacc acagaaccac aagtgcggtg caaactttct
       61 ccaggaagac tgcaagaagg ctctggcgtt taaatggtta atctctgcag gtcactacca
      121 gccaccgaga ccaaccgagt cagtgagtgc tctaaccaca gtccatgcag gaatagtagg
      181 tccttcaaat atttgctcac tccgttttgt tttgtttcct tgcttttcac atgttaccag
      241 ctacataatt tcttgacaga aaaaaataaa tataaagtct atgtactcca ggcatactgt
      301 acaactaaaa cagggactgg gtatggtttg tattttcagt ttaaggctgc aagcagtatt
      361 tacaacagag ggcacaagtt ctatctggaa aaaaaaggag ggactatggc gtcaaacagc
      421 ctcttcagcg cagtgacacc gtgtcagcaa agcttctttt gggatccgag caccagccgg
      481 cgcttcagcc ccccctccag cagcctgcag cccggcaaga tgagcgacgt gagcccggtg
      541 gtggctgcgc agcagcagca acagcagcag cagcagcagc aacagcagca gcaacaacag
      601 caacagcaac aacagcagca gcagcagcag cagcaggagg cggccgcagc agcagcggcg
      661 gcagcggcgg cggcagcagc ggcggcggcc gcagtgcccc gattgaggcc gccgcacgac
      721 aaccgcacca tggtggagat catcgcggac cacccggccg aactggtccg caccgacagt
      781 cccaacttcc tgtgctccgt gctgccctcg cactggcggt gcaacaagac cctgcccgtg
      841 gccttcaagg ttgtagccct cggagaggta ccagatggga ctgtggttac cgtcatggcc
      901 gggaatgatg agaactactc cgccgagctc cgaaatgcct ccgctgttat gaaaaaccaa
      961 gtagccaggt tcaacgatct gagatttgtg ggccggagcg gacgaggcaa gagtttcacc
     1021 ttgaccataa cagtcttcac aaatcctccc caagtggcca cttaccacag agctattaaa
     1081 gtgacagtgg acggtccccg ggaaccaaga aggcacagac agaagcttga tgactctaaa
     1141 cctagtttgt tctctgatcg cctcagtgat ttagggcgca ttcctcatcc cagtatgaga
     1201 gtaggtgtcc cgcctcagaa cccacggccc tccctgaact ctgcaccaag tccttttaat
     1261 ccacaaggac agagtcagat tacagatccc aggcaggcac agtcttcccc accgtggtcc
     1321 tatgaccagt cttacccctc ctatctgagc cagatgacat ccccatccat ccactccacc
     1381 acgccgctgt cttccacacg gggcaccggg ctacctgcca tcactgacgt gcccaggcgt
     1441 atttcagatg atgacactgc cacctctgac ttctgcctct ggccttcctc tctcagtaag
     1501 aagagccagg caggtgcttc agaactgggc cctttttcag accccaggca gttcccaagc
     1561 atttcatccc tcactgagag ccgcttctcc aacccacgaa tgcactaccc agccaccttt
     1621 acctacaccc cgccagtcac gtcaggcatg tccctcggca tgtccgccac cactcactac
     1681 cacacgtacc tgccaccacc ctaccccggc tcttcccaaa gccagagtgg acccttccag
     1741 accagcagca ctccatatct ctactatggt acttcgtcag catcctatca gttcccaatg
     1801 gtacccgggg gagaccggtc tccttccagg atggtcccac catgcaccac cacctcgaat
     1861 ggcagcacgc tattaaatcc aaatttgcct aaccagaatg atggtgttga cgctgacgga
     1921 agccacagca gttccccaac tgttttgaat tctagcggca gaatggatga gtctgtttgg
     1981 cggccatatt gaaattcgtc aaccatggcc cagtggcatg ggggccacat cccgcatgtg
     2041 ttaatatata catatataaa gagagtgcct atatatgtat attgattagc taactagaag
     2101 atttctcatt caatccctag tcatgatctt gcaaccctaa gagggtgggg gcagtcataa
     2161 ctgggtttca tattgtttac tatttaagat gtccccttta ccaaggaaca aaccgtcaaa
     2221 ggtgttgtct ggtctgtttt cataagtgac ctgttcccac gccggttcag agaggtggac
     2281 tctgggtctg ggaggaagga gagacacttc ctctctgtgc tttgaaacca cagcctctgc
     2341 tgtgtggcag ccggtacact ctgcagaccc gcttacagag tcagatgtgg tgcactcaga
     2401 aagggacaag aggcagagtg gctgcttctg tccgctgccg tccactctgc cgtccacctg
     2461 ttccaaagtt ttccttcaga cttgctgcag gtactcattt gaacttttga gttcactttt
     2521 tttttttcct attctaagaa agtgacttca aaaatactga tcaggacaga taattttatt
     2581 ttacctttta tattttctca cttcccccat ttaaccaaaa agaaatcccg ttccccctcc
     2641 cccgttcctt ctgcttctcc ctttatgcaa actgaaaatg gcaatgcctt attattatag
     2701 ccataatggt atagtgtttg agttggctgt gtgttatgtg tttttttctt tttttttctt
     2761 ttttaaatta tgaatatgtg taaaatctga agtaacttgc taacgtgaat ggtcatataa
     2821 ctttaaagat atatttataa ttatttaatg acatttggac atttggaaca tttcttagtg
     2881 taatgatatg ttgacttcgg tctctaaaag tgtgcttctt cttcaatacc aagtttcttc
     2941 agtgggctag agccatatcg gaaatattgc taagcaatct caattccttc aggcataatg
     3001 tgattttttt ttttttttga agataactcc catctccaaa tagtttagat gtagtttgtt
     3061 ttcacgatgt atgaaggaga tgctctgttt ctttctttca ggcatttgat tgcctctgac
     3121 acagctttgc cttttaaagc aataattagg gattaaaata acaaaaacaa aacaaaagcc
     3181 acctatagcc ctttaacact taacgtggcc cctttactag catgaaatgc tggagacatg
     3241 tggtttccta atttctccat tttgggggtg gtgggagggg ggagggtggc cattatgact
     3301 cttatcatat taaaaagcca agcacaagtg attggttgaa ctgcagaaaa gtgttctgtg
     3361 gtctctgagt tgagcaaaac tctaaattgc aggcttcgtg gttgagggcc tagtcagctg
     3421 aaagccacgc gtgtggtaaa ggctcaggca tggcttggag aacctaggaa cacattagga
     3481 gcctgcacct accagcctca ccatacagcc attcagggga acccaaaaag tgccttaccc
     3541 aaggagggcc cccagcagct ttccaggaag tcgaatgaag tcgctgtcct cggggaactg
     3601 gtcagctgaa gtagcaaccg gtagctgatg tcagtagaca acagaacctg tggggacctc
     3661 caggaaacct ttgacattgg aggctttcat taggcagggc caacaagagc agggaaggcc
     3721 atgtacccat tggtatctgc cattgtgtgt gagtttgaga tccagccctc cttggaggat
     3781 gtactgtgat cattcctggt accttagggc caatccctaa gtgtggcttc ctaatccagg
     3841 ccagggatca ttcagtgata ccaccaggcc aatcccagca ttcctcctgc acaaagtgtt
     3901 tgtgtgtggg gggatagatt ggggtggggc caccttcatt tgaatcctga gtcattctaa
     3961 gagttctgca agcttttgcc ttcagcaccc tataccccct cgctctctgt tccttctcag
     4021 gttgaccttt gtcccaatgc gggacagtcc agaggcagat ggggacctat gtgtgcctcc
     4081 aacctgcgtt ttcctcagaa ttcatcctga ctccctctga cacagattga gggggggggg
     4141 aagaaaccca acccgcacca aagcaaaaca ctttgccttc taaaggctgt gcaccaagta
     4201 gacgcagatg gtcagcccac ctttgtgttt ccttaagatg gaaattgtaa ctgatgctat
     4261 ttattgtttg tgtgtggtag cttgaagcac accacggtcc atgtgtttgt ctgataccta
     4321 tttcagcagc atttacaaaa gccagtgtct ggttacactt ttcagttttc attaatcaac
     4381 atgaaaatgt taccattggt gccagctgca ccctatttaa tttttttaag ggcactatat
     4441 ttgtacattt cgtttttaat gttaaagggc ttcttaaagt ttacagtaca gttatcaagg
     4501 gaatagaggg gatgcattag tgcctaaatg ttattctagt ggctgcaggc agcaacccag
     4561 aagcagtttt gaaaacaggt tgtttccctc tgtcctccct tatttgggaa aattcaagtg
     4621 ctttcttcac ctttcaggca cctcacggtg actcccgtta cttagagcag tctgtcgtcg
     4681 tcttcttctt cttcctcttc ctcttcctct tcctcttcct cttcctcttc ctcttcctct
     4741 tcctcttcct cttcttcttc ttcttcttct tcttcttctt cttcttcttc ctcctagggt
     4801 ttaaaatccc ccttcctctt cccttatctc ttaaactctt catttgcact gggtcacacg
     4861 tatgatttgt ggttttaaga ccaaagcaat gtcttattac tcttctggag ccgtttatgt
     4921 gtactaacca acccttccct ccacttccct gggtttagat gcacacggtt ctcaaaggag
     4981 cacaaacatg gccagattca cagtgggacc cacacagcca tgttaaaaaa aaaaacaaaa
     5041 aaaaacctct tcacttgtct gagaatttta acctgggccc caattgctaa cgggcaccat
     5101 ggcttgggtt tcaggttagg gaacgttgcc cagtgagtaa cagaaagact taactgattt
     5161 aattagtttg ccctcatcct tcactccaag accctaagaa accgatcaga gaaacatctc
     5221 cacaccatta gaggttgaga agggaggagc catgtggggg ttccctccgc tcggaggaaa
     5281 ggcactgact gacctagtta gagtggtagc agaagcaccc atggtatctg agagagcaat
     5341 aaacaataaa agatgatcct cccaagctta gaatttgttg ttcttaaaga agacgatgca
     5401 tctaaaacaa agaatttttt tttctatgtg ggaactttct tcgttgtctg tttacttcat
     5461 gtttacaaat aattgtttgc tttttaatgc agtactttaa aaaatatatc agccaaaacc
     5521 ataacttaca gtaatttttt taggtattct gaataaaatt ccatgtcttt tgatatgtct
     5581 actgttctta ggtttctgtg gaacaacaac aacaacaaca acaaaattgt agcattttgt
     5641 gtaaatacag ctttcgttct tattttttat ttttctgatt gctattgccc aagatttgct
     5701 ttctatgcac gtattgtaca aattgtgctt tgtgccacag gtcatgatcg tggatgagtt
     5761 tactctgaac ttcaaaggga ctatttgtat tgtatgttgc aactgtaaat tgaattattt
     5821 ggcatttccc cctctcatga ttgtaatatt aatttgaagt ttgaatttaa ttttcaataa
     5881 aaaggctttt tttttc
//
