LOCUS       NM_001111022            5611 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus runt related transcription factor 1 (Runx1),
            transcript variant 2, mRNA.
ACCESSION   NM_001111022
VERSION     NM_001111022.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Chen,X., Tober,J., Dominguez,M., Tang,A.T., Bockman,J., Yang,J.,
            Mani,S., Lee,C.N., Chen,M., Thillaikumaran,T., Mericko-Ishizuka,P.,
            Mainigi,M., Speck,N.A. and Kahn,M.L.
  TITLE     Lineage tracing studies suggest that the placenta is not a de novo
            source of hematopoietic stem cells
  JOURNAL   PLoS Biol 23 (1), e3003003 (2025)
   PUBMED   39874373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   3  (bases 1 to 5611)
  AUTHORS   Niibori-Nambu,A., Wang,C.Q., Chin,D.W.L., Chooi,J.Y., Hosoi,H.,
            Sonoki,T., Tham,C.Y., Nah,G.S.S., Cirovic,B., Tan,D.Q.,
            Takizawa,H., Sashida,G., Goh,Y., Tng,J., Fam,W.N., Fullwood,M.J.,
            Suda,T., Yang,H., Tergaonkar,V., Taniuchi,I., Li,S., Chng,W.J. and
            Osato,M.
  TITLE     Integrin-alpha9 overexpression underlies the niche-independent
            maintenance of leukemia stem cells in acute myeloid leukemia
  JOURNAL   Gene 928, 148761 (2024)
   PUBMED   39002785
REFERENCE   4  (bases 1 to 5611)
  AUTHORS   Cai,S. and Yin,N.
  TITLE     Single-cell transcriptome and chromatin accessibility mapping of
            upper lip and primary palate fusion
  JOURNAL   J Cell Mol Med 28 (19), e70128 (2024)
   PUBMED   39392189
REFERENCE   5  (bases 1 to 5611)
  AUTHORS   Wilken,M.B., Fonar,G., Qiu,R., Bennett,L., Tober,J., Nations,C.,
            Pavani,G., Tsao,V., Garifallou,J., Petit,C., Maguire,J.A.,
            Gagne,A., Okoli,N., Gadue,P., Chou,S.T., French,D.L., Speck,N.A.
            and Thom,C.S.
  TITLE     Tropomyosin 1 deficiency facilitates cell state transitions and
            enhances hemogenic endothelial cell specification during
            hematopoiesis
  JOURNAL   Stem Cell Reports 19 (9), 1264-1276 (2024)
   PUBMED   39214082
REFERENCE   6  (bases 1 to 5611)
  AUTHORS   Sun,W., Graves,B.J. and Speck,N.A.
  TITLE     Transactivation of the Moloney murine leukemia virus and T-cell
            receptor beta-chain enhancers by cbf and ets requires intact
            binding sites for both proteins
  JOURNAL   J Virol 69 (8), 4941-4949 (1995)
   PUBMED   7609063
REFERENCE   7  (bases 1 to 5611)
  AUTHORS   Simeone,A., Daga,A. and Calabi,F.
  TITLE     Expression of runt in the mouse embryo
  JOURNAL   Dev Dyn 203 (1), 61-70 (1995)
   PUBMED   7647375
REFERENCE   8  (bases 1 to 5611)
  AUTHORS   Calabi,F., Rhodes,M., Williamson,P. and Boyd,Y.
  TITLE     Identification and chromosomal mapping of a third mouse runt-like
            locus
  JOURNAL   Genomics 26 (3), 607-610 (1995)
   PUBMED   7607689
REFERENCE   9  (bases 1 to 5611)
  AUTHORS   Miyoshi,H., Shimizu,K., Kozu,T., Maseki,N., Kaneko,Y. and Ohki,M.
  TITLE     t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are
            clustered within a limited region of a single gene, AML1
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10431-10434 (1991)
   PUBMED   1720541
REFERENCE   10 (bases 1 to 5611)
  AUTHORS   Kania,M.A., Bonner,A.S., Duffy,J.B. and Gergen,J.P.
  TITLE     The Drosophila segmentation gene runt encodes a novel nuclear
            regulatory protein that is also expressed in the developing nervous
            system
  JOURNAL   Genes Dev 4 (10), 1701-1713 (1990)
   PUBMED   2249771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT025774.19, AL672278.12 and BQ033862.1.
            
            On Dec 21, 2013 this sequence version replaced NM_001111022.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the coding region compared to variant 1. It encodes isoform
            2 which is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC069929.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               CT025774.19        67331-67471
            142-257             CT025774.19        67701-67816
            258-511             CT025774.19        197668-197921
            512-668             CT025774.19        204273-204429
            669-773             AL672278.12        182578-182682       c
            774-935             AL672278.12        127469-127630       c
            936-5197            AL672278.12        115733-119994       c
            5198-5611           BQ033862.1         1-414               c
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 53.7 cM"
     gene            1..5611
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="runt related transcription factor 1"
                     /db_xref="GeneID:12394"
                     /db_xref="MGI:MGI:99852"
     exon            1..141
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            142..257
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1405
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="isoform 2 is encoded by transcript variant 2;
                     oncogene AML-1; acute myeloid leukemia 1 protein;
                     core-binding factor subunit alpha-2; SL3-3 enhancer factor
                     1 alpha B subunit; SL3/AKV core-binding factor alpha B
                     subunit; polyomavirus enhancer-binding protein 2 alpha B
                     subunit; core binding factor alpha 2; runt domain, alpha
                     subunit 2"
                     /codon_start=1
                     /product="runt-related transcription factor 1 isoform 2"
                     /protein_id="NP_001104492.1"
                     /db_xref="GeneID:12394"
                     /db_xref="MGI:MGI:99852"
                     /translation="MASDSIFESFPSYPQCFMRDASTSRRFTPPSTALSPGKMSEALP
                     LGAPDGGAALASKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIA
                     FKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSF
                     TLTITVFTNPPQVATYHRAIKITVDGPREPRNARQIQPSPPWSYDQSYQYLGSITSSS
                     VHPATPISPGRASGMTSLSAELSSRLSTAPDLTAFGDPRQFPTLPSISDPRMHYPGAF
                     TYSPPVTSGIGIGMSAMSSASRYHTYLPPPYPGSSQAQAGPFQTGSPSYHLYYGASAG
                     SYQFSMVGGERSPPRILPPCTNASTGAALLNPSLPSQSDVVETEGSHSNSPTNMPPAR
                     LEEAVWRPY"
     exon            258..511
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            512..668
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            669..773
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            774..935
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            936..5594
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4968..4973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="hexamer: AACAAG"
     polyA_site      4984
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="major polyA site"
     regulatory      5571..5576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="hexamer: AATAAA"
     polyA_site      5590
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="major polyA site"
ORIGIN      
        1 gtgaaaactt ctttggacct cataaacaac cacagaacca caagttgggt agcctggcag
       61 tgtcagaagt gtaagcccag cacagtggtc agcaggcagg acgaatcaca ctgaatgcaa
      121 accacaggct ttcgcagagc ggtgaaagaa attatagaat cccccgcctt caggagaggt
      181 gcgttttcga aaggaaacga tggcttcaga cagcattttt gagtcatttc cttcatatcc
      241 acagtgcttc atgagagatg ccagcacgag ccgccgcttc acgccgcctt ccaccgcgct
      301 gagccccggc aagatgagcg aggcgctgcc gctgggcgcc ccggatggcg gcgccgccct
      361 ggccagcaag ctgaggagcg gcgaccgcag catggtggag gtactagctg accaccctgg
      421 cgagctagtg cgcaccgaca gccccaactt cctctgctcc gtgctaccca ctcactggcg
      481 ctgcaacaag accctgccca tcgctttcaa ggtggtggca ctgggggacg tcccggatgg
      541 cactctggtc accgtcatgg caggcaacga tgaaaactac tcggcagaac tgagaaatgc
      601 taccgcggcc atgaagaacc aggtagcgag attcaacgac ctcaggtttg tcgggcggag
      661 cggtagaggc aagagcttca ctctgaccat caccgtcttt acaaatccgc cacaagttgc
      721 cacctaccat agagccatca aaatcacagt ggacggcccc cgagaacccc gaaatgccag
      781 gcagatccag ccatccccac cgtggtccta tgaccagtcc taccagtacc tgggatccat
      841 cacctcttcc tctgtccacc cagcgacacc catttcaccc ggccgtgcca gcggcatgac
      901 cagcctctct gcagaacttt ccagtcgact ctcaacggct ccggacctga ccgccttcgg
      961 cgacccacgc cagttcccta ctctgccgtc catctccgac ccgcgcatgc actacccagg
     1021 cgccttcacc tactcgccgc ccgtcacgtc gggcatcggc atcggcatgt cagccatgag
     1081 ctcggcctct cgctaccaca cctacctgcc gccgccctac cccggctcat cacaggcgca
     1141 ggccgggccc ttccagaccg gctcgccctc ctaccatcta tactacggcg cctcggccgg
     1201 ttcctaccag ttctccatgg tgggcggaga gagatcgccc ccgcgcatcc tgccgccctg
     1261 caccaacgca tccaccggcg ccgcgctgct caaccccagc ctccccagcc agagcgacgt
     1321 ggtggagacc gagggcagcc atagcaactc gcccaccaac atgccccccg cgcgcctgga
     1381 ggaggccgtg tggcggccct actgagctga gcgccatcgc catcgaggga ctgggcctgc
     1441 cgtccatgca cagaccccgc caggagggcc cttggaggcc accaggaaga atcccggagg
     1501 gaaactgtga atgcttctga tttagcaatg ctgtgaataa aagaaagatt ttataccctt
     1561 gacttcactt tttaaccacg ttgtttattc caaagagtgt ggaatgtttt cggttcgggg
     1621 tggggaagac gcagcccatc ctgtttggca tctatttctt atttcggagt tttcttttcc
     1681 gcaccttatc gattgcaaaa atgcctgttt gcatctgggt ggtcatttat ttttaagtgt
     1741 gtatagattt gagcttgctt ttttttcttc ctttgaccaa ctcaaagaaa taaaattccc
     1801 ttctctgtaa ggtttattta acttttagac tttcatgtag ctgggggttt tatttgtgtt
     1861 tggtttttgt ttttattttt aaagagacag ctacagcttt gggtcatttt ttaactactg
     1921 tatttccaca aagaaatccc tagatattta tgtatcttga tgtttgaaca tttacatatg
     1981 tgttgatact tttttaatta tttaaatgta cttatattaa gaaagatatc aagtactaca
     2041 tttttcttta taatagccaa agttaaatat tattgcgttg aagatgtctg gaaaaaaaag
     2101 agatcgcttg gttaactaga aatattgttt acattaaact ccctttatgt tattcaaaca
     2161 agttggtagg taacgcagca atgtttttaa ttggattgta gacactgagg gtcactccaa
     2221 ggtcagaagt acaaaatttt ctgctaggct caacaaatag tctcatacct ggctccttcc
     2281 cttcaaaaag agaggcaaac tctgtcctga aagggttcag agaggtgcca aggatttgct
     2341 ctgaagagga tttcattttg gcctggagat atacttgccc caaggcctcc tcattctggc
     2401 atgctttatc acagagctca accaagtaag ctgttggtca ggggtttact tacatagtat
     2461 ttacatagac ccaaaccact gaatgtgatt tttaaattgc cttccattaa tagtacccgt
     2521 tcattgatga aaaccaaaac ttgaggctgt accccaaaga tccaaataga agagttaaga
     2581 ccaggtgtct ttgaggccta aaggctgagt tttaagagag tgtaccccaa aagtctgaag
     2641 gagccggttt ccttctccca gtcttagtgg aatcagtcat gggaggcaga tgccacgccc
     2701 acctgtgcag gatgctcctc agaagctgcc ccttcaccag catcttctcc caccaggccg
     2761 agcccctgac ctttggggtg catcagtgtg atagatcctg gtctctgcag tccgccatgg
     2821 ctacggttca gatgtgcatc gtgtcactgt aaatgtaatg gtactgttgt tacagtggag
     2881 gacttggtca aaatccagtt gttctacaac gtatgaagcc taaccgctgg ttctgacata
     2941 catgtgctca aaatgatctg gttgtttgga tttttctttt gttgttttgt tttttaatgt
     3001 acctcttaaa ttagttgaag tgatgtcagg tcaactccga agagcgtttg aaagcaggac
     3061 ttcagcacag tgtttgattt ttttattatt attaatatta ttttataaat ttaagcattc
     3121 agattagatc tttggctgca ggcagcaaaa acggctggac ttatttaaaa aaaatacagc
     3181 ttgttttttg agttatctat atctatatct atatgttgat tctttgtctt acatagagca
     3241 gcagcacttt ggtaacctgt gataccaggt tgctcttgtc tggagaagag cgctagcagg
     3301 attcagagaa actcagaata gatcttcata tcagccatac cttcctcctc catccggtct
     3361 ccactcagtt attccacaga acactttgac agctgtgttg tcagaaaaat aaaaaaaaat
     3421 ttaatttctc aaaaggagtt tgtttctcca acattagatg ttcctcttac cataggctgc
     3481 cgtatctggc ctgagaaaac ggtagggaag gacgaaggaa agagatttct attttttcat
     3541 attaattttg atatctaaag atacgctagc cctcagagga gcagataatc tcacacattg
     3601 aattttcgcc ctgggcacca tgcatcaaga aggcttgtca ctgtgttaga gccatttagt
     3661 gcttcctaaa cttttatcaa cataggcagt atttagtctc agagaaaaaa aaatccatca
     3721 ggcacatgta gtcttggaga tagattccac ggggcaggta tttctctacc tgagaaattg
     3781 tgttcattgc cttcgggtgc ttccagcggt ctcctcattc gctgtcttca aggaagaccc
     3841 ataagccaat tctgagataa tggagctgtt gggaatactg gtccagagaa agaaaaatgg
     3901 gataagccat tcttactgct tattcaagcc cctatttata attttaacac actttccatt
     3961 ccttctggtt ttctcgccgt ctatatcctc ccaatagccc ttctcacttt tcttttccct
     4021 cctgcaaaca cacacacaca cacacacaca cacacacata aggcacacac acacacatcc
     4081 tctcccccat accaagtgtc cagaacacag aaagtccagt tcttctccgt ttattaaaga
     4141 acagggtgag tcagccattc tcttgctcac gggttttttt ccccaacaga acagaggcgt
     4201 tgccagccat tttgggtctg ctttctgtcc agatactgca gcaaaaactc ttgaggatca
     4261 caacccgttg gctgagcagc tgtgctgctg cccaaacgtc ctgcgcagac aaacgcacgc
     4321 tgggaccgga aggggtgtct ctccttctgc ctcttttctt tcatacgttt ctctcgaaag
     4381 gcctcaactg aggactgcaa atttctttct tgaaataact ttcccccagg gacattcggt
     4441 cttagggatt ttttggtttt gatgggtttt gttttgtttt ggtttttttg gttcttctca
     4501 ttttctttgt aggagaaggc atgagatgtt gagggtcttt catacatgaa aataaatagt
     4561 ttgacagcaa tctcagaata tattttttcc ttatttgaac aaagtactgt tttgtttact
     4621 ctacagtaca cctttatttg gtgggtttgg ctgttggtcg gaaatgcctt ccctctttca
     4681 gccaggtcgg tatagccaaa gctgatcatt tgtctgtttt ttcttttttt tcctcagaag
     4741 agttgtatcc acaaactcaa ttgtatttat gtatgtaaat agatttcatg cttcattata
     4801 aaatattgtt aatgcctgta ataacttttt tcacattttg tgtgtgtttc taaggacttt
     4861 ttcttatgtt cgctaaatat tgtagaagaa aaatgcttct cttaacttgt ttattttaga
     4921 ttttaaaaaa accaagctac ttcttactca cttttataaa cataataaac aagcatggtt
     4981 ccaatgggtt ttttttaagt tcaccttatg ttctaggaga aatgaatgtg caaaaaaaaa
     5041 aatcaatctt caaatgaact gttgggtatt tctgttactc tggatggaaa aagatcaatg
     5101 gaaaacaaaa aacaaaaaaa caaacttcca aattgaaata gcagtaaaac acatgtattg
     5161 ggggtaaaaa aaaaaaaaaa aaacacacct cagaaaatgc agttctccag cctccagcta
     5221 ctgcccacac atgtggcaag tgttagatgt ggtcgtgagg aatcccaaaa tcagtgcccg
     5281 ggccttttcc ttcctggtac taacattaag aacacctcct aaggggcact gtcaggctca
     5341 ggtcttaaag cacatagata agagagaatg tcccagaacg attctcagta gcccaggcca
     5401 gcaggtactc acggcactgc acctgctcac tgcacacagt cgctgaggca gacccctctc
     5461 ctgggggcca tgcagagcca gggcagggca gccctaagct actgatgttt tctttccttt
     5521 gtactatggt cttttctgta actccaatat tcaaacccat gtgaatggca aataaacaat
     5581 ttgacaagca cctaaaaaaa aaaaaaaaaa a
//
