LOCUS       NM_001111021            5803 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus runt related transcription factor 1 (Runx1),
            transcript variant 1, mRNA.
ACCESSION   NM_001111021
VERSION     NM_001111021.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Chen,X., Tober,J., Dominguez,M., Tang,A.T., Bockman,J., Yang,J.,
            Mani,S., Lee,C.N., Chen,M., Thillaikumaran,T., Mericko-Ishizuka,P.,
            Mainigi,M., Speck,N.A. and Kahn,M.L.
  TITLE     Lineage tracing studies suggest that the placenta is not a de novo
            source of hematopoietic stem cells
  JOURNAL   PLoS Biol 23 (1), e3003003 (2025)
   PUBMED   39874373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   3  (bases 1 to 5803)
  AUTHORS   Niibori-Nambu,A., Wang,C.Q., Chin,D.W.L., Chooi,J.Y., Hosoi,H.,
            Sonoki,T., Tham,C.Y., Nah,G.S.S., Cirovic,B., Tan,D.Q.,
            Takizawa,H., Sashida,G., Goh,Y., Tng,J., Fam,W.N., Fullwood,M.J.,
            Suda,T., Yang,H., Tergaonkar,V., Taniuchi,I., Li,S., Chng,W.J. and
            Osato,M.
  TITLE     Integrin-alpha9 overexpression underlies the niche-independent
            maintenance of leukemia stem cells in acute myeloid leukemia
  JOURNAL   Gene 928, 148761 (2024)
   PUBMED   39002785
REFERENCE   4  (bases 1 to 5803)
  AUTHORS   Cai,S. and Yin,N.
  TITLE     Single-cell transcriptome and chromatin accessibility mapping of
            upper lip and primary palate fusion
  JOURNAL   J Cell Mol Med 28 (19), e70128 (2024)
   PUBMED   39392189
REFERENCE   5  (bases 1 to 5803)
  AUTHORS   Wilken,M.B., Fonar,G., Qiu,R., Bennett,L., Tober,J., Nations,C.,
            Pavani,G., Tsao,V., Garifallou,J., Petit,C., Maguire,J.A.,
            Gagne,A., Okoli,N., Gadue,P., Chou,S.T., French,D.L., Speck,N.A.
            and Thom,C.S.
  TITLE     Tropomyosin 1 deficiency facilitates cell state transitions and
            enhances hemogenic endothelial cell specification during
            hematopoiesis
  JOURNAL   Stem Cell Reports 19 (9), 1264-1276 (2024)
   PUBMED   39214082
REFERENCE   6  (bases 1 to 5803)
  AUTHORS   Sun,W., Graves,B.J. and Speck,N.A.
  TITLE     Transactivation of the Moloney murine leukemia virus and T-cell
            receptor beta-chain enhancers by cbf and ets requires intact
            binding sites for both proteins
  JOURNAL   J Virol 69 (8), 4941-4949 (1995)
   PUBMED   7609063
REFERENCE   7  (bases 1 to 5803)
  AUTHORS   Simeone,A., Daga,A. and Calabi,F.
  TITLE     Expression of runt in the mouse embryo
  JOURNAL   Dev Dyn 203 (1), 61-70 (1995)
   PUBMED   7647375
REFERENCE   8  (bases 1 to 5803)
  AUTHORS   Calabi,F., Rhodes,M., Williamson,P. and Boyd,Y.
  TITLE     Identification and chromosomal mapping of a third mouse runt-like
            locus
  JOURNAL   Genomics 26 (3), 607-610 (1995)
   PUBMED   7607689
REFERENCE   9  (bases 1 to 5803)
  AUTHORS   Miyoshi,H., Shimizu,K., Kozu,T., Maseki,N., Kaneko,Y. and Ohki,M.
  TITLE     t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are
            clustered within a limited region of a single gene, AML1
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10431-10434 (1991)
   PUBMED   1720541
REFERENCE   10 (bases 1 to 5803)
  AUTHORS   Kania,M.A., Bonner,A.S., Duffy,J.B. and Gergen,J.P.
  TITLE     The Drosophila segmentation gene runt encodes a novel nuclear
            regulatory protein that is also expressed in the developing nervous
            system
  JOURNAL   Genes Dev 4 (10), 1701-1713 (1990)
   PUBMED   2249771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK040240.1, AK137493.1, CT025774.19, AL672278.12 and BQ033862.1.
            
            On Dec 21, 2013 this sequence version replaced NM_001111021.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1, also known as AML1-C; PMID 11243869).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK137493.1, AK145098.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AK040240.1         2-32
            32-608              AK137493.1         3-579
            609-609             CT025774.19        204370-204370
            610-3268            AK137493.1         580-3238
            3269-3269           AL672278.12        117853-117853       c
            3270-3905           AK137493.1         3240-3875
            3906-3907           AL672278.12        117215-117216       c
            3908-5223           AK137493.1         3879-5194
            5224-5389           AL672278.12        115733-115898       c
            5390-5803           BQ033862.1         1-414               c
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 53.7 cM"
     gene            1..5803
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="runt related transcription factor 1"
                     /db_xref="GeneID:12394"
                     /db_xref="MGI:MGI:99852"
     exon            1..141
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            142..257
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1597
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="isoform 1 is encoded by transcript variant 1;
                     oncogene AML-1; acute myeloid leukemia 1 protein;
                     core-binding factor subunit alpha-2; SL3-3 enhancer factor
                     1 alpha B subunit; SL3/AKV core-binding factor alpha B
                     subunit; polyomavirus enhancer-binding protein 2 alpha B
                     subunit; core binding factor alpha 2; runt domain, alpha
                     subunit 2"
                     /codon_start=1
                     /product="runt-related transcription factor 1 isoform 1"
                     /protein_id="NP_001104491.1"
                     /db_xref="CCDS:CCDS49917.1"
                     /db_xref="GeneID:12394"
                     /db_xref="MGI:MGI:99852"
                     /translation="MASDSIFESFPSYPQCFMRDASTSRRFTPPSTALSPGKMSEALP
                     LGAPDGGAALASKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIA
                     FKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSF
                     TLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQL
                     RRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDARQIQPSPPWSYDQSYQYLG
                     SITSSSVHPATPISPGRASGMTSLSAELSSRLSTAPDLTAFGDPRQFPTLPSISDPRM
                     HYPGAFTYSPPVTSGIGIGMSAMSSASRYHTYLPPPYPGSSQAQAGPFQTGSPSYHLY
                     YGASAGSYQFSMVGGERSPPRILPPCTNASTGAALLNPSLPSQSDVVETEGSHSNSPT
                     NMPPARLEEAVWRPY"
     exon            258..511
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            512..668
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            669..773
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            774..965
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1127
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..5786
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5160..5165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="hexamer: AACAAG"
     polyA_site      5176
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="major polyA site"
     regulatory      5763..5768
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="hexamer: AATAAA"
     polyA_site      5782
                     /gene="Runx1"
                     /gene_synonym="AML1; CBF-alpha-2; Cbfa2; Pebp2a2; Pebpa2b"
                     /note="major polyA site"
ORIGIN      
        1 gtgaaaactt ctttggacct cataaacaac cacagaacca caagttgggt agcctggcag
       61 tgtcagaagt gtaagcccag cacagtggtc agcaggcagg acgaatcaca ctgaatgcaa
      121 accacaggct ttcgcagagc ggtgaaagaa attatagaat cccccgcctt caggagaggt
      181 gcgttttcga aaggaaacga tggcttcaga cagcattttt gagtcatttc cttcatatcc
      241 acagtgcttc atgagagatg ccagcacgag ccgccgcttc acgccgcctt ccaccgcgct
      301 gagccccggc aagatgagcg aggcgctgcc gctgggcgcc ccggatggcg gcgccgccct
      361 ggccagcaag ctgaggagcg gcgaccgcag catggtggag gtactagctg accaccctgg
      421 cgagctagtg cgcaccgaca gccccaactt cctctgctcc gtgctaccca ctcactggcg
      481 ctgcaacaag accctgccca tcgctttcaa ggtggtggca ctgggggacg tcccggatgg
      541 cactctggtc accgtcatgg caggcaacga tgaaaactac tcggcagaac tgagaaatgc
      601 taccgcggcc atgaagaacc aggtagcgag attcaacgac ctcaggtttg tcgggcggag
      661 cggtagaggc aagagcttca ctctgaccat caccgtcttt acaaatccgc cacaagttgc
      721 cacctaccat agagccatca aaatcacagt ggacggcccc cgagaacccc gaagacatcg
      781 gcagaaacta gatgatcaga ccaagcccgg gagtttgtcc ttttccgagc ggctcagtga
      841 attggagcag ctgcggcgca cggccatgag ggtcagcccg caccacccag cccccacgcc
      901 caaccctcgg gcctccttga accactccac tgcctttaac cctcagcctc aaagtcagat
      961 gcaggatgcc aggcagatcc agccatcccc accgtggtcc tatgaccagt cctaccagta
     1021 cctgggatcc atcacctctt cctctgtcca cccagcgaca cccatttcac ccggccgtgc
     1081 cagcggcatg accagcctct ctgcagaact ttccagtcga ctctcaacgg ctccggacct
     1141 gaccgccttc ggcgacccac gccagttccc tactctgccg tccatctccg acccgcgcat
     1201 gcactaccca ggcgccttca cctactcgcc gcccgtcacg tcgggcatcg gcatcggcat
     1261 gtcagccatg agctcggcct ctcgctacca cacctacctg ccgccgccct accccggctc
     1321 atcacaggcg caggccgggc ccttccagac cggctcgccc tcctaccatc tatactacgg
     1381 cgcctcggcc ggttcctacc agttctccat ggtgggcgga gagagatcgc ccccgcgcat
     1441 cctgccgccc tgcaccaacg catccaccgg cgccgcgctg ctcaacccca gcctccccag
     1501 ccagagcgac gtggtggaga ccgagggcag ccatagcaac tcgcccacca acatgccccc
     1561 cgcgcgcctg gaggaggccg tgtggcggcc ctactgagct gagcgccatc gccatcgagg
     1621 gactgggcct gccgtccatg cacagacccc gccaggaggg cccttggagg ccaccaggaa
     1681 gaatcccgga gggaaactgt gaatgcttct gatttagcaa tgctgtgaat aaaagaaaga
     1741 ttttataccc ttgacttcac tttttaacca cgttgtttat tccaaagagt gtggaatgtt
     1801 ttcggttcgg ggtggggaag acgcagccca tcctgtttgg catctatttc ttatttcgga
     1861 gttttctttt ccgcacctta tcgattgcaa aaatgcctgt ttgcatctgg gtggtcattt
     1921 atttttaagt gtgtatagat ttgagcttgc ttttttttct tcctttgacc aactcaaaga
     1981 aataaaattc ccttctctgt aaggtttatt taacttttag actttcatgt agctgggggt
     2041 tttatttgtg tttggttttt gtttttattt ttaaagagac agctacagct ttgggtcatt
     2101 ttttaactac tgtatttcca caaagaaatc cctagatatt tatgtatctt gatgtttgaa
     2161 catttacata tgtgttgata cttttttaat tatttaaatg tacttatatt aagaaagata
     2221 tcaagtacta catttttctt tataatagcc aaagttaaat attattgcgt tgaagatgtc
     2281 tggaaaaaaa agagatcgct tggttaacta gaaatattgt ttacattaaa ctccctttat
     2341 gttattcaaa caagttggta ggtaacgcag caatgttttt aattggattg tagacactga
     2401 gggtcactcc aaggtcagaa gtacaaaatt ttctgctagg ctcaacaaat agtctcatac
     2461 ctggctcctt cccttcaaaa agagaggcaa actctgtcct gaaagggttc agagaggtgc
     2521 caaggatttg ctctgaagag gatttcattt tggcctggag atatacttgc cccaaggcct
     2581 cctcattctg gcatgcttta tcacagagct caaccaagta agctgttggt caggggttta
     2641 cttacatagt atttacatag acccaaacca ctgaatgtga tttttaaatt gccttccatt
     2701 aatagtaccc gttcattgat gaaaaccaaa acttgaggct gtaccccaaa gatccaaata
     2761 gaagagttaa gaccaggtgt ctttgaggcc taaaggctga gttttaagag agtgtacccc
     2821 aaaagtctga aggagccggt ttccttctcc cagtcttagt ggaatcagtc atgggaggca
     2881 gatgccacgc ccacctgtgc aggatgctcc tcagaagctg ccccttcacc agcatcttct
     2941 cccaccaggc cgagcccctg acctttgggg tgcatcagtg tgatagatcc tggtctctgc
     3001 agtccgccat ggctacggtt cagatgtgca tcgtgtcact gtaaatgtaa tggtactgtt
     3061 gttacagtgg aggacttggt caaaatccag ttgttctaca acgtatgaag cctaaccgct
     3121 ggttctgaca tacatgtgct caaaatgatc tggttgtttg gatttttctt ttgttgtttt
     3181 gttttttaat gtacctctta aattagttga agtgatgtca ggtcaactcc gaagagcgtt
     3241 tgaaagcagg acttcagcac agtgtttgat ttttttatta ttattaatat tattttataa
     3301 atttaagcat tcagattaga tctttggctg caggcagcaa aaacggctgg acttatttaa
     3361 aaaaaataca gcttgttttt tgagttatct atatctatat ctatatgttg attctttgtc
     3421 ttacatagag cagcagcact ttggtaacct gtgataccag gttgctcttg tctggagaag
     3481 agcgctagca ggattcagag aaactcagaa tagatcttca tatcagccat accttcctcc
     3541 tccatccggt ctccactcag ttattccaca gaacactttg acagctgtgt tgtcagaaaa
     3601 ataaaaaaaa atttaatttc tcaaaaggag tttgtttctc caacattaga tgttcctctt
     3661 accataggct gccgtatctg gcctgagaaa acggtaggga aggacgaagg aaagagattt
     3721 ctattttttc atattaattt tgatatctaa agatacgcta gccctcagag gagcagataa
     3781 tctcacacat tgaattttcg ccctgggcac catgcatcaa gaaggcttgt cactgtgtta
     3841 gagccattta gtgcttccta aacttttatc aacataggca gtatttagtc tcagagaaaa
     3901 aaaaatccat caggcacatg tagtcttgga gatagattcc acggggcagg tatttctcta
     3961 cctgagaaat tgtgttcatt gccttcgggt gcttccagcg gtctcctcat tcgctgtctt
     4021 caaggaagac ccataagcca attctgagat aatggagctg ttgggaatac tggtccagag
     4081 aaagaaaaat gggataagcc attcttactg cttattcaag cccctattta taattttaac
     4141 acactttcca ttccttctgg ttttctcgcc gtctatatcc tcccaatagc ccttctcact
     4201 tttcttttcc ctcctgcaaa cacacacaca cacacacaca cacacacaca taaggcacac
     4261 acacacacat cctctccccc ataccaagtg tccagaacac agaaagtcca gttcttctcc
     4321 gtttattaaa gaacagggtg agtcagccat tctcttgctc acgggttttt ttccccaaca
     4381 gaacagaggc gttgccagcc attttgggtc tgctttctgt ccagatactg cagcaaaaac
     4441 tcttgaggat cacaacccgt tggctgagca gctgtgctgc tgcccaaacg tcctgcgcag
     4501 acaaacgcac gctgggaccg gaaggggtgt ctctccttct gcctcttttc tttcatacgt
     4561 ttctctcgaa aggcctcaac tgaggactgc aaatttcttt cttgaaataa ctttccccca
     4621 gggacattcg gtcttaggga ttttttggtt ttgatgggtt ttgttttgtt ttggtttttt
     4681 tggttcttct cattttcttt gtaggagaag gcatgagatg ttgagggtct ttcatacatg
     4741 aaaataaata gtttgacagc aatctcagaa tatatttttt ccttatttga acaaagtact
     4801 gttttgttta ctctacagta cacctttatt tggtgggttt ggctgttggt cggaaatgcc
     4861 ttccctcttt cagccaggtc ggtatagcca aagctgatca tttgtctgtt ttttcttttt
     4921 tttcctcaga agagttgtat ccacaaactc aattgtattt atgtatgtaa atagatttca
     4981 tgcttcatta taaaatattg ttaatgcctg taataacttt tttcacattt tgtgtgtgtt
     5041 tctaaggact ttttcttatg ttcgctaaat attgtagaag aaaaatgctt ctcttaactt
     5101 gtttatttta gattttaaaa aaaccaagct acttcttact cacttttata aacataataa
     5161 acaagcatgg ttccaatggg ttttttttaa gttcacctta tgttctagga gaaatgaatg
     5221 tgcaaaaaaa aaaatcaatc ttcaaatgaa ctgttgggta tttctgttac tctggatgga
     5281 aaaagatcaa tggaaaacaa aaaacaaaaa aacaaacttc caaattgaaa tagcagtaaa
     5341 acacatgtat tgggggtaaa aaaaaaaaaa aaaaacacac ctcagaaaat gcagttctcc
     5401 agcctccagc tactgcccac acatgtggca agtgttagat gtggtcgtga ggaatcccaa
     5461 aatcagtgcc cgggcctttt ccttcctggt actaacatta agaacacctc ctaaggggca
     5521 ctgtcaggct caggtcttaa agcacataga taagagagaa tgtcccagaa cgattctcag
     5581 tagcccaggc cagcaggtac tcacggcact gcacctgctc actgcacaca gtcgctgagg
     5641 cagacccctc tcctgggggc catgcagagc cagggcaggg cagccctaag ctactgatgt
     5701 tttctttcct ttgtactatg gtcttttctg taactccaat attcaaaccc atgtgaatgg
     5761 caaataaaca atttgacaag cacctaaaaa aaaaaaaaaa aaa
//
