LOCUS       NM_027425               7374 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus RUN and FYVE domain-containing 2 (Rufy2), mRNA.
ACCESSION   NM_027425
VERSION     NM_027425.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7374)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7374)
  AUTHORS   Wei,Z., Sun,M., Liu,X., Zhang,J. and Jin,Y.
  TITLE     Rufy3, a protein specifically expressed in neurons, interacts with
            actin-bundling protein Fascin to control the growth of axons
  JOURNAL   J Neurochem 130 (5), 678-692 (2014)
   PUBMED   24720729
REFERENCE   5  (bases 1 to 7374)
  AUTHORS   Nakano,Y., Jahan,I., Bonde,G., Sun,X., Hildebrand,M.S.,
            Engelhardt,J.F., Smith,R.J., Cornell,R.A., Fritzsch,B. and Banfi,B.
  TITLE     A mutation in the Srrm4 gene causes alternative splicing defects
            and deafness in the Bronx waltzer mouse
  JOURNAL   PLoS Genet 8 (10), e1002966 (2012)
   PUBMED   23055939
REFERENCE   6  (bases 1 to 7374)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7374)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   8  (bases 1 to 7374)
  AUTHORS   Yang,J., Kim,O., Wu,J. and Qiu,Y.
  TITLE     Interaction between tyrosine kinase Etk and a RUN domain- and FYVE
            domain-containing protein RUFY1. A possible role of ETK in
            regulation of vesicle trafficking
  JOURNAL   J Biol Chem 277 (33), 30219-30226 (2002)
   PUBMED   11877430
REFERENCE   9  (bases 1 to 7374)
  AUTHORS   Dunkelberg,J.C. and Gutierrez-Hartmann,A.
  TITLE     LZ-FYVE: a novel developmental stage-specific leucine zipper,
            FYVE-finger protein
  JOURNAL   DNA Cell Biol 20 (7), 403-412 (2001)
   PUBMED   11506704
REFERENCE   10 (bases 1 to 7374)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113107.15.
            
            On Jan 23, 2019 this sequence version replaced NM_027425.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK173195.1, BC137737.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AC113107.15        89700-89780
            82-255              AC113107.15        92119-92292
            256-373             AC113107.15        98571-98688
            374-475             AC113107.15        100428-100529
            476-599             AC113107.15        102565-102688
            600-661             AC113107.15        103428-103489
            662-727             AC113107.15        104153-104218
            728-797             AC113107.15        104758-104827
            798-899             AC113107.15        107329-107430
            900-1016            AC113107.15        107527-107643
            1017-1184           AC113107.15        109681-109848
            1185-1282           AC113107.15        111631-111728
            1283-1402           AC113107.15        114105-114224
            1403-1532           AC113107.15        116073-116202
            1533-1627           AC113107.15        117122-117216
            1628-1676           AC113107.15        118804-118852
            1677-1754           AC113107.15        120866-120943
            1755-7374           AC113107.15        121038-126657
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 32.51 cM"
     gene            1..7374
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="RUN and FYVE domain-containing 2"
                     /db_xref="GeneID:70432"
                     /db_xref="MGI:MGI:1917682"
     exon            1..81
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1898
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="leucine zipper FYVE-finger protein"
                     /codon_start=1
                     /product="RUN and FYVE domain-containing protein 2"
                     /protein_id="NP_081701.2"
                     /db_xref="CCDS:CCDS48582.1"
                     /db_xref="GeneID:70432"
                     /db_xref="MGI:MGI:1917682"
                     /translation="MATKDPTAVERANLLNMAKLSIKGLIESALSFGRTLDSDYPPLQ
                     QFFVVMEHCLKHGLKGRKSFLSYNKTIWGPLELVEKLYPEAEEIGASVRDLPGLKTPL
                     GRARAWLRLALMQKKMADYLRCLIIQRELLSEFYEYHALMMEEEGAVIVGLLVGLNVI
                     DANLCVKGEDLDSQVGVIDFSMYLKNEEEIGNKERNVQIAAILDQKNYVEELNRQLNS
                     TVSSLHSRVDSLEKSNTKLIEELAIAKNNIIKLQEENHQLRSENELILMRTRQHLEVT
                     KVDVETELQTYKHSRQGLDEMYNDARRQLRDESQLRQDVENELSVQVGMKHEMELAMK
                     LLEKDIHEKQDTLIGLRQQLEEVKAINIEMYQRLQGSEDGLKEKNEIIARLEEKTNKI
                     TTAMRQLEQRLQQAEKAQKEAEAEDEKYAQECLSQSDSLQRQISQKEQQLVQLETDLK
                     IEKEWRQTLQEDLQKEKDVLSHLRHETQKVISLKKEFLNLQDENQQLKRIYQEQEQAL
                     QELGSKLCESKLKIDDIKEANKALQGLVWLKDKDATHCKLCEKEFSLSKRKHHCRNCG
                     EIFCNACSDNELPLPSSPKPVRVCDSCHAMLIQRCSSNMP"
     exon            82..255
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            256..373
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            374..475
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            476..599
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            600..661
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            662..727
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            728..797
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            798..899
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1016
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1184
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1282
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1402
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1532
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1627
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1676
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1754
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..7374
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4095..4100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: AATAAA"
     regulatory      4110..4115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: TATAAA"
     polyA_site      4120
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="major polyA site"
     regulatory      4126..4131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: AAGAAA"
     polyA_site      4135
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
     polyA_site      4158
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
     regulatory      6788..6793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: AGTAAA"
     polyA_site      6810
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
     regulatory      7038..7043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: ACTAAA"
     polyA_site      7076
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
     regulatory      7221..7226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
                     /note="hexamer: AATAAA"
     polyA_site      7244
                     /gene="Rufy2"
                     /gene_synonym="2610111M19Rik; Denn; LZ-FYVE; ZFYVE13"
ORIGIN      
        1 agacgcggtg gccgccgcgc gcctgcgggg agctgctgac ccgctccgcg agcccgtgtc
       61 ccgcacgcac cgccgccatg gctacaaagg accccacagc cgtggagaga gccaacctgt
      121 taaacatggc taaactgagt atcaaagggc tcatcgagtc tgccctgagc ttcggccgca
      181 cgctggactc tgactaccct cctctgcagc agttcttcgt ggtcatggaa cattgtctga
      241 aacacggcct gaaagggaga aaatcatttt tgagctataa caaaactatt tggggccccc
      301 tggaactggt ggagaagctg tacccagaag cagaggagat cggggcgagt gtccgggact
      361 tgcctgggct taagactccg cttggccgtg ctagagcgtg gctccgatta gccctcatgc
      421 agaaaaaaat ggccgattac ttgcgttgct taattatcca gagggagctc ctaagtgagt
      481 tctatgagta ccatgcactg atgatggagg aggagggagc cgtgattgtc gggctgctgg
      541 tcggcttgaa cgtgattgat gccaatctgt gtgtgaaggg agaggaccta gactcccagg
      601 ttggggtgat tgacttctcc atgtacttaa agaatgaaga agagatcgga aacaaggaaa
      661 ggaacgttca aatcgccgcc atcttagacc aaaagaatta tgttgaagag ctaaacagac
      721 aactcaacag cacagtcagc agcctccatt caagagtgga ctcgttagaa aagtcaaaca
      781 ctaagctgat cgaagagtta gcaatagcga agaataatat cataaagctc caagaagaaa
      841 accatcaatt gcgtagtgaa aatgagctga tcttaatgag aactcggcag cacctggagg
      901 ttaccaaagt ggatgtagaa acggagcttc aaacatacaa gcattcccgg caaggcctag
      961 acgagatgta taacgatgcc aggaggcagc ttcgagacga gtcgcagctc cgacaggatg
     1021 tggagaatga gctatcagta caagttggca tgaagcatga gatggagctg gccatgaagc
     1081 tgttggagaa agacattcac gagaaacaag acactctcat aggccttcgg caacagctgg
     1141 aagaagtcaa agcaatcaac attgagatgt atcaaaggct gcagggctct gaagatggct
     1201 tgaaagaaaa aaatgaaata attgcccgac tagaagaaaa gaccaataag atcactacag
     1261 ccatgaggca gctggagcaa agattgcagc aagcagagaa ggcgcaaaag gaagcagaag
     1321 ccgaggatga gaaatatgcc caggagtgtc tgagccaatc cgacagtctg cagagacaga
     1381 tctcgcagaa ggagcaacag ctggtgcagc tggaaacgga cttgaagatc gagaaggaat
     1441 ggagacagac tttgcaagaa gatcttcaaa aggaaaaaga tgtcctatct catcttagac
     1501 atgaaaccca aaaagtcata agtcttaaaa aagagtttct taacctccag gatgaaaatc
     1561 agcagttgaa aaggatttat caggaacaag aacaagctct gcaagaactt ggcagcaaac
     1621 tctgcgaatc gaaacttaaa atagacgata taaaagaagc caacaaagca ctacagggac
     1681 tggtctggtt aaaagacaaa gatgcaaccc actgtaagct ttgtgagaag gaattttcac
     1741 tctccaaaag aaagcaccac tgtagaaact gcggagaaat tttctgtaat gcctgctctg
     1801 acaacgagct gcctttgcct tcttcaccaa agccagtgcg agtctgtgat tcctgccatg
     1861 caatgctcat tcagagatgc tcctctaaca tgccatgact tggaactcca ttctcctgca
     1921 cagcaccacc tgcagcgagg gagggctgtg tgcccagtca gtactgtacc ttgcctccag
     1981 ctcacaaaca ggtttctaag ttatatgggt tagtattccg tccctgtact taatcagatt
     2041 ttaagatagc atgttttagc actacttcaa ctatcaagta acctttaaca aaaggtcaga
     2101 tagcagagct aatgcacatt tgccttacct tatagaggcc tttttgagaa caaggccaca
     2161 gcttatttgc ttctgaactg atgagtaggc acttgaaggt gcctgtctct taatgcactt
     2221 tagaaagcgt cacaattctc agagaaaaac tggaaatgca ttcactttat gttatatgcc
     2281 gatttaacgg tttttaaaat ggtggcccac gccttcaatc ccagcactta gatgcagagg
     2341 caggtggatg gatctgagtt cagtaaggcc accctggtag aaagaggaag ttccagtcag
     2401 gacagccaga ctgtccagga agacagactg aatcaaagtt gtttattttt taccataagc
     2461 tgtcttgcct aagctctgaa gtgctgggat ataggtgtgt gcttccatcc caggtctgtg
     2521 tagtcttcat tgtctggtaa gatgctaatt tagttgtatt tatttgcttg ttcttctccc
     2581 ttctctacaa tggaaaagac caatgattta atcccttcct ttccttttta cttgcccaca
     2641 tctttcatat agtggactaa aggaccagtg gtctggcctc tagcttttcc ttcacttata
     2701 atcatcatcc ttatcacaaa tactaatttt tataaagggt cagattccaa aggagaaaac
     2761 attcctaccc agggaacaag gggaccgaat gagaccacag gcctttaaag attgattcta
     2821 agcattaaaa ttaagaggac tgctatgaaa gaattaagtt tctccaccct cagttctctt
     2881 aagaataact tctaggaagc attacactgg catatttacg cttgcccaca gtagaatttc
     2941 accctctatg tgtattaggt actttagata gtagctcctt attttttcaa gcgggggtgt
     3001 gtgtgggaga cacggagcat taagttactt aaatcatgac catttaggat aggatcttta
     3061 atatattaat ttccagcctt atacttttaa gtctataaag cctttcaaaa ctctagtgat
     3121 tttacactcc caagaacact gctcagtgac tgggtatttt tctgatcccc ttttctgcag
     3181 gatcttttca tttgctacat ttcagtaagt ttttgtaaat tgccaaaatt acaaaaccag
     3241 tatttaccag cttctgtggt caacttaaat tcactccgac agatttctag atgtttcttt
     3301 tggagacagg gaaaccccaa gttggcctcc aacctggtgt gcagcagggg aagaccttga
     3361 actgcctgac ttccacatct cccagctgct gagattacag ctgttgagtg tttgtatgtg
     3421 gtgctgggga tggagcccct gatactttgt acacattggc caggtactct actaactcag
     3481 ttactctccc aaccccagat gtcattatat atatatatat atatgtatgt atgtatgtgt
     3541 gtgtgtgtat atatatatat atatatatat atatatatat atatatatat atatatatat
     3601 atatatatat agtgaaaacc tgctggcaca cacacaaaaa agagacaatt taactgcttt
     3661 gtactgatgg ggattactag catctttctt tctttctttc tttttttttt tttgagtgta
     3721 aatgaaaaac ttcaaatact ttattatgtg ctttttatta ccactcaaaa tttgtgtcat
     3781 aaacatgggc cttttctgga gcagcactaa cgagttcccc tgctgtgtgt gagataagaa
     3841 tagagctgag tggtagaatg gttgcttatc gtggctatta agggcctgga ttctcaattc
     3901 acagcactac caccaaaaaa cataaaacca cccaacttta aatttaggat atcctattta
     3961 ttacataaca ttcacaattc ttattttgca gaaaactcgt gtcattccct tacttaccta
     4021 gccagtacta ccacaaagat gtgatcatgg tgccaaggaa ttgcaaatgt aattctgatc
     4081 tgtaatgttt ttaaaataaa tgtgaatttt ataaatcgaa gatctaagaa attaacactt
     4141 acttatattg ctttaaaaga cttgtttgta tctgcttatg tgtgtgtgaa tgtgtgccat
     4201 gtgtgtgcag gtgaccaacg aggccaggaa aaggcattgg acttaagcga ggctgatcac
     4261 ctgatgtggg tgctgggaac caaacggccc tctgaaagag cagcgagtac tctttattta
     4321 tttatgtata tgagtacact gtagctgact tcagacacac cagaagaggg caacagatcc
     4381 tgttacagag ggctgagcct ccgtgtggtt gctgggaatt gaacgcagga cctggaagag
     4441 cagccagtgc tttaaccgcc gagcatctct ccagcccgca gcacgtactc ttaaccactc
     4501 tacctactga agtactcttt agccacctgc tttgagatgt aagaagcttg aagtgcttgt
     4561 gacttctgtg cgaggtgtgc aggcaccaag ttaaacccat taagacagaa actcactgtt
     4621 tcaggccgac aggctccaag tgacatgctg tggcaactct ctcttcttag catgggggaa
     4681 aaaaaaatca cgcctttgta gttacttgat cagaaacctc aatttggcat ccaacagtat
     4741 attgcctttg cttcccaaat gctgggatta aaggcatggt gatacttgat accaataact
     4801 aacacaacat attctaggac agacaacatt cccatgttga catttattac attaataaca
     4861 tatctagcaa aaaacaagta actcaataac tgagatcaca ctgacaggtt cattttgatc
     4921 cattagccaa tttggggaaa agtcttattg agcaaagtaa taaaaagatt agtagaaagc
     4981 acgttttcct tctacacagg gcacttaact actcaaagcc agtttcagac gcaaaaccag
     5041 ctacagcatt gttttatagt ctcctaaatg tagtcattta taccattata aagaccctgt
     5101 tttcttgggt gagaaactga aaatacaagt aagcatatac ccattatagt aaatgcccgt
     5161 gtatttacag agtttttctg tcattattaa gaatttcaaa ctactccaac aaactcaagt
     5221 tttgggaacc atttttggta tcttagtcaa gtgctatgag ggttttatac ccctaccccc
     5281 attcataata caatttgtga atatttagaa tccagatacg tcttgaatta ttcagagcta
     5341 tgcttgataa aagagaccga aaagaataca gaattagctt atgttcctga aaccacctga
     5401 acgtatcttt aggcttttta tttacagtat gtccaggggt gaaaggtagt gctaccctag
     5461 atgcctactt aagactcgct gtcttgccct ctatcactgc actatgctaa cagtaagata
     5521 cattatccca aatcttatga aggcagcaca tctttaggag tacctatata taaaaagtag
     5581 caacatcagt ggtttgaata taaaaattta tttaagtcaa agtatgcaac aaatgaaaca
     5641 acctacagaa aacagatttt cccatcgcag tctgttgctt taccaaataa tataacacat
     5701 tccttcagtc gttataaagt tcttgaaata caaaagaaat gaaatctgta agaaagtcta
     5761 gtagagcagg tgtgttagga cgtgtacgtt ggggtggtgt tgtcagtaca tcccacgcca
     5821 tccacctcca ctcattccac cttgcccata gtaacctcca ctgcctccac caccacgacc
     5881 ttaagaagac agtaagaacc gtccatcagg agacatccat gtctttaaat gaaggacact
     5941 accacaatac agaaacaact acttaccata gcctcccaag ccatcaggag caccgtagcc
     6001 tccgctgtag ctgttcccca tccccattct cccaacagac ccgtatcctc cttgattatc
     6061 tgcacacaga aaagaagcca cagaggatga ctgccttgtg gagtggccaa cacttgctca
     6121 ttctacacaa tttacaaacc taggtttgga aggatatgct gaagcatcaa ggagaattgt
     6181 gagttttggg ccatcctgtg ttacagtgag gtgctaccca tcacaaatac aaaactatac
     6241 atacccattc catctctgcc atagcctccc attccagaac ctcccattcc agagccacct
     6301 ccaggagtag aattcaagaa gagttcaatg tatcgatgtt cttttaaaaa aaaaaaaaaa
     6361 gatgggtgga aaaggtaaga ggattagaga aaggaatgct agatcaaagg tattaaagtt
     6421 aatttataaa actaattcaa acagccacta aagtttaagc attattagta ggtcaaatga
     6481 ttttaaaccc tagggtctac tacaggcact ttaacagtat ttcataaact gcacacacct
     6541 gtaaaacaag cataacaaag ataccactta cgcatgttgt ttttatcttt agacatggca
     6601 gctactgcat cttcatgtgt cacaaactct acatctgcct cgcctgttgc tctgccatcg
     6661 gctccaatgt caatatgaac acgtattgga ttgagcggtg agaagaacta aacagaagtt
     6721 attcatttta aaattccagt tatttaaagt tagcatatac ttcaattttt tttccctagt
     6781 ccactaaagt aaactactga gagtctaaaa gctgcagtat tgtagaggta ggctagtcat
     6841 cctttgctat ggaagtggtc aacttagctc acacagggaa ggagtacata ttttaaaatg
     6901 ccaaaagtgc tgctgcttta aactaaggta atagtacatt ttaagtgaaa actgtccaac
     6961 tgtgggatcc agctcccctt tagagaaagt gtgcattatc aggggtatct gagtttggga
     7021 atgcttcaga gcctactact aaaagcaagc tgcttcctga ctgactcttc ttcacacatg
     7081 ggtgttgttt atttttaatg catttttttt aacctgctta aaattctcat taggtcagca
     7141 tataatgggc acaaaacatc tatgtgcaca ttaggcaaat aatcaatcac taagtgacaa
     7201 ccttagcctg agtctcaaca aataaaaaag atgagtcagc tgggcatggt ggctgcctgt
     7261 aattccaaca tgctcaaagc caagttagca ttagcaaatt ctagcccagt ttggcctccc
     7321 cactacaagt aattactact aacaaaaaaa ataataaata cccactataa aagc
//
