LOCUS       NM_198642               2651 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus RUN and cysteine rich domain containing beclin 1
            interacting protein like (Rubcnl), transcript variant 1, mRNA.
ACCESSION   NM_198642 XM_283220 XM_354828
VERSION     NM_198642.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Cheng,X., Ma,X., Zhu,Q., Song,D., Ding,X., Li,L., Jiang,X.,
            Wang,X., Tian,R., Su,H., Shen,Z., Chen,S., Liu,T., Gong,W., Liu,W.
            and Sun,Q.
  TITLE     Pacer Is a Mediator of mTORC1 and GSK3-TIP60 Signaling in
            Regulation of Autophagosome Maturation and Lipid Metabolism
  JOURNAL   Mol Cell 73 (4), 788-802 (2019)
   PUBMED   30704899
  REMARK    GeneRIF: mTORC1 and GSK3-TIP60 signaling converge to modulate
            autophagosome maturation and lipid metabolism through Pacer.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC146617.3.
            
            On Aug 22, 2007 this sequence version replaced NM_198642.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155201.1, AK170426.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-140               AC146617.3         158352-158491
            141-749             AC146617.3         174197-174805
            750-832             AC146617.3         177744-177826
            833-1034            AC146617.3         178335-178536
            1035-1136           AC146617.3         180664-180765
            1137-1197           AC146617.3         181197-181257
            1198-1330           AC146617.3         183128-183260
            1331-1476           AC146617.3         184662-184807
            1477-1541           AC146617.3         186734-186798
            1542-1780           AC146617.3         189737-189975
            1781-1842           AC146617.3         190523-190584
            1843-1996           AC146617.3         191889-192042
            1997-2087           AC146617.3         192427-192517
            2088-2651           AC146617.3         194294-194857
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 39.53 cM"
     gene            1..2651
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /note="RUN and cysteine rich domain containing beclin 1
                     interacting protein like"
                     /db_xref="GeneID:271221"
                     /db_xref="MGI:MGI:2685590"
     exon            1..140
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            141..749
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..2200
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein RUBCNL-like; pacer; protein Rubicon-like; protein
                     associated with UVRAG as autophagy enhancer"
                     /codon_start=1
                     /product="protein associated with UVRAG as autophagy
                     enhancer isoform 1"
                     /protein_id="NP_941044.3"
                     /db_xref="CCDS:CCDS36977.1"
                     /db_xref="GeneID:271221"
                     /db_xref="MGI:MGI:2685590"
                     /translation="MNSRVTPSGTTAIGELCFEHVHSIPGKMVSQSIGWGDSPVDLCE
                     GSNRAFQDTDQPACQLDVRLLRHKASWINPLCVQQPLQELCPQRPTVQSSENHVVLDT
                     PSPLRLSILSYRDSLAEMPLSENTADVICSNSAHCSGGKEGDFFLATEEQEVHLQQES
                     LLKNPKTVATSPSPKEGSARSESPHLTASTDDGDARSSSRSHAWNFFPLETFMLPADV
                     EKENLHFYAADIIISVIENMKCNLPNQQQPERWDTEDASRLRGTGAEMTFYTHIKQEP
                     GSSASSHTGCEGCAALQVSPVAETLSYCPVAGEACKHDLNKLVMLELGKYNDITKGCR
                     CSYNSSKSATCESNLSPAGCLARELFRGFCKCWMLSEVNCQLPGSPTTASSGVGDEEY
                     AEEDFDSSVDAAREVMLKSRVPGTEDWVLPRCQIILTVHPPIKRDIAVVAQNFFCAGC
                     GTPIQPKFVKRLRYCEYLGKYFCASCHSSAESCIPARILTMWDFRKYQVSDFSKWLLD
                     SVWHQPVFKLLGGHHSLYAKAKELDRVKDLQEQLFHIKKLLKTCRFADSVLKEFEQVP
                     SHLTDECHIFSMDDFLRTKKGLLAPLLKDILRASLAHVDSCELCQGKGFICEFCQSTT
                     VIFPFQTTTCRRCAACRACFHKQCFQSSRCPRCARIIARRQHLESLPTAAT"
     exon            750..832
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            833..1034
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1136
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1197
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1330
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1476
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1541
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1780
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1842
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1996
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2087
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2651
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2507..2512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /note="hexamer: AATACA"
     polyA_site      2536
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /note="major polyA site"
     polyA_site      2644
                     /gene="Rubcnl"
                     /gene_synonym="5031414D18Rik; Gm744; Gm911; Kiaa0226l"
                     /note="major polyA site"
ORIGIN      
        1 aaactttctg tattcgttgg cagcttctct ctgcaccatc agaacctcgg agctgttggc
       61 acacggagga ggtgatccga acctgtcgct gccacggggc aaggtggcta tttggtggat
      121 aaaacggcca ggaggcgtag ataaactctt cagacctcca gctggctatg tgatgaattc
      181 aagagtcacg cccagtggta caacggccat tggagagctg tgttttgagc acgttcactc
      241 catacctgga aaaatggtgt cccagtccat aggctgggga gactctcctg tggacctctg
      301 tgaagggagc aaccgtgcct tccaggacac tgaccaacct gcttgccaat tggatgtgag
      361 gctcctaaga cacaaagctt cctggatcaa cccgctgtgt gtgcagcagc cacttcaaga
      421 attatgcccc caaagaccaa ccgtgcagag cagtgagaac cacgtggtgc tggacactcc
      481 ctctcccttg cgcctttcga tattgtccta cagggactcc ctggctgaga tgccactgtc
      541 tgagaatact gctgatgtca tctgcagcaa ctctgctcat tgctccgggg gaaaggaggg
      601 tgacttcttc ctggctacag aagagcaaga ggttcatctt cagcaggaat ctctgctcaa
      661 aaaccccaag actgtggcca cttccccctc ccctaaggag ggcagtgccc gttctgagtc
      721 tcctcatttg acagcttcta cagatgatgg tgatgctcgg agcagcagca gaagccatgc
      781 ttggaatttc ttcccactag agacgttcat gctgcccgct gatgtagaaa aggaaaatct
      841 ccacttttat gctgcagaca tcattatctc agtaatagag aatatgaagt gcaacctccc
      901 gaatcaacag cagccagaga gatgggacac agaagacgcc agcaggttac gtgggactgg
      961 agcggaaatg accttttata cccatataaa gcaggagcca ggctcctcgg cttcttcaca
     1021 cactggctgt gaaggttgtg ctgcactcca ggtcagccca gtggctgaga ccctttctta
     1081 ctgccctgtg gcaggagagg cctgcaaaca tgacttgaac aagttggtca tgttagaact
     1141 tgggaaatat aatgacatca cgaaaggctg cagatgctcc tataactctt caaagagtgc
     1201 gacctgcgag tcaaacctca gtcctgctgg gtgcttagcc agagagttgt tccgagggtt
     1261 ctgtaagtgc tggatgctgt cagaagttaa ttgccagctg ccaggctcac cgaccacagc
     1321 tagctcagga gtcggggatg aagaatatgc tgaggaagac tttgactcca gtgtggatgc
     1381 tgctcgggaa gtcatgctta agtctagggt cccaggaact gaagactggg tgctccctcg
     1441 atgccaaatc atacttactg ttcacccacc aatcaagagg gacatagcag tggtggccca
     1501 gaacttcttc tgtgctggat gtgggactcc aatacagccc aagtttgtga agaggctccg
     1561 gtactgtgag tatctcggga agtacttctg tgccagctgc cactcatctg cagagtcttg
     1621 tatccctgcg cggatcctga cgatgtggga cttcaggaag taccaagtca gcgatttctc
     1681 taaatggctc ttggacagcg tatggcatca acctgtcttc aaactgttag gtggccatca
     1741 cagcctctat gcaaaagcta aggaactgga tagggtgaag gaccttcagg agcaactttt
     1801 tcatatcaag aagctattga agacttgcag gtttgctgac agtgtgctga aggagtttga
     1861 gcaagttccc agccacttga ctgatgaatg ccacatattc tccatggacg acttcctcag
     1921 gaccaagaaa ggactgttgg cacctttgct taaagacatc ctgagagctt ctcttgccca
     1981 cgtggacagc tgtgagctgt gccaggggaa gggcttcatt tgtgagtttt gtcagagcac
     2041 gacggtcatc ttcccatttc agaccacgac ttgtagaaga tgtgcagcgt gcagggcctg
     2101 ctttcacaaa cagtgtttcc agtcttcgag atgcccgcgc tgtgccagga tcatagcccg
     2161 gagacaacat ttggagagct tgcccactgc agcaacatga tggcccaaag ttgttccaaa
     2221 cctctcatga tgttaccaca ttgtgtctgc tcctggtaac attcattgtt tcagatacta
     2281 agtatttttg tagtaagaaa aagaggacca aaattgccct cttgtacgca tttaatgctt
     2341 tcaaggagta tggaagtttt gttattaggt atagggttgt tgataagggt ttgtttacaa
     2401 tgtttgacat tgttttgctg ctactggctg tttttttttt tttttaaaga agtctttttt
     2461 catttaagtt gcactgggat aggttttttt tttttttttg aagctaaata cagaacctat
     2521 ttttttatga acttaactga caaggtgatg tgttgtttgt gaaatggatt ggcgccacaa
     2581 aggagggaag tggcacaaga attctgtttg gccaagcttt tgttaaagac gactgttgaa
     2641 tgttgctttg t
//
