LOCUS       NM_172615               5316 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus RUN domain and cysteine-rich domain containing, Beclin
            1-interacting protein (Rubcn), transcript variant 2, mRNA.
ACCESSION   NM_172615 XM_003085099
VERSION     NM_172615.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Sun,L., Wu,S., Wang,H., Zhang,T., Zhang,M., Bai,X., Zhang,X.,
            Li,B., Zhang,C., Li,Y., Zhou,J. and Li,T.
  TITLE     PDCD6 regulates lactate metabolism to modulate LC3-associated
            phagocytosis and antibacterial defense
  JOURNAL   Nat Commun 15 (1), 10157 (2024)
   PUBMED   39578445
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Nguyen,A.T., Masuda,M., Mori,Y., Adachi,Y., Fukuda,T., Furuichi,A.,
            Takikawa,M., Tsuda,Y., Hamada,Y., Maruyama,Y., Ohminami,H.,
            Ohnishi,K. and Taketani,Y.
  TITLE     All-trans retinoic acid induces lipophagy by reducing Rubicon in
            Hepa1c1c7 cells
  JOURNAL   J Lipid Res 65 (8), 100598 (2024)
   PUBMED   39032560
  REMARK    GeneRIF: All-trans retinoic acid induces lipophagy by reducing
            Rubicon in Hepa1c1c7 cells.
REFERENCE   3  (bases 1 to 5316)
  AUTHORS   Feng,S., McNehlan,M.E., Kinsella,R.L., Sur Chowdhury,C.,
            Chavez,S.M., Naik,S.K., McKee,S.R., Van Winkle,J.A., Dubey,N.,
            Samuels,A., Swain,A., Cui,X., Hendrix,S.V., Woodson,R.,
            Kreamalmeyer,D., Smirnov,A., Artyomov,M.N., Virgin,H.W., Wang,Y.T.
            and Stallings,C.L.
  TITLE     Autophagy promotes efficient T cell responses to restrict high-dose
            Mycobacterium tuberculosis infection in mice
  JOURNAL   Nat Microbiol 9 (3), 684-697 (2024)
   PUBMED   38413834
REFERENCE   4  (bases 1 to 5316)
  AUTHORS   Paudel,B., Jeong,S.Y., Martinez,C.P., Rickman,A., Haluck-Kangas,A.,
            Bartom,E.T., Fredriksen,K., Affaneh,A., Kessler,J.A.,
            Mazzulli,J.R., Murmann,A.E., Rogalski,E., Geula,C., Ferreira,A.,
            Heckmann,B.L., Green,D.R., Sadleir,K.R., Vassar,R. and Peter,M.E.
  TITLE     Death Induced by Survival gene Elimination (DISE) correlates with
            neurotoxicity in Alzheimer's disease and aging
  JOURNAL   Nat Commun 15 (1), 264 (2024)
   PUBMED   38238311
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5316)
  AUTHORS   Li,Y., Chen,Y., Xiao,X., Deng,S., Kuang,J. and Wang,Y.
  TITLE     CX3CL1 represses autophagy via CX3CR1/ CaMKIIdelta/HDAC4/Rubicon
            axis and exacerbates chronic intermittent hypoxia induced Kupffer
            cell apoptosis
  JOURNAL   Cell Signal 111, 110873 (2023)
   PUBMED   37640194
  REMARK    GeneRIF: CX3CL1 represses autophagy via CX3CR1/
            CaMKIIdelta/HDAC4/Rubicon axis and exacerbates chronic intermittent
            hypoxia induced Kupffer cell apoptosis.
REFERENCE   6  (bases 1 to 5316)
  AUTHORS   Matsunaga,K., Saitoh,T., Tabata,K., Omori,H., Satoh,T.,
            Kurotori,N., Maejima,I., Shirahama-Noda,K., Ichimura,T., Isobe,T.,
            Akira,S., Noda,T. and Yoshimori,T.
  TITLE     Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally
            regulate autophagy at different stages
  JOURNAL   Nat Cell Biol 11 (4), 385-396 (2009)
   PUBMED   19270696
  REMARK    GeneRIF: Two Beclin 1 associated proteins, Atg14L and Rubicon, were
            identified.
REFERENCE   7  (bases 1 to 5316)
  AUTHORS   Zhong,Y., Wang,Q.J., Li,X., Yan,Y., Backer,J.M., Chait,B.T.,
            Heintz,N. and Yue,Z.
  TITLE     Distinct regulation of autophagic activity by Atg14L and Rubicon
            associated with Beclin 1-phosphatidylinositol-3-kinase complex
  JOURNAL   Nat Cell Biol 11 (4), 468-476 (2009)
   PUBMED   19270693
  REMARK    GeneRIF: Beclin 1 and its binding proteins orchestrate the precise
            function of the class III PI(3)K in regulating autophagy at
            multiple steps.
REFERENCE   8  (bases 1 to 5316)
  AUTHORS   Zanivan,S., Gnad,F., Wickstrom,S.A., Geiger,T., Macek,B., Cox,J.,
            Fassler,R. and Mann,M.
  TITLE     Solid tumor proteome and phosphoproteome analysis by high
            resolution mass spectrometry
  JOURNAL   J Proteome Res 7 (12), 5314-5326 (2008)
   PUBMED   19367708
REFERENCE   9  (bases 1 to 5316)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   10 (bases 1 to 5316)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139244.4, AK030368.1, BC067390.1 and BP758360.1.
            
            On or before Jan 7, 2011 this sequence version replaced
            XM_003085099.1, NM_172615.3.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the coding region, compared to variant 1. This results in a
            shorter protein (isoform b), compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030368.1, BC067390.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC139244.4         160459-160485       c
            28-4126             AK030368.1         2-4100
            4127-4127           BC067390.1         4112-4112
            4128-5264           AK030368.1         4102-5238
            5265-5316           BP758360.1         1-52                c
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 23.38 cM"
     gene            1..5316
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /note="RUN domain and cysteine-rich domain containing,
                     Beclin 1-interacting protein"
                     /db_xref="GeneID:100502698"
                     /db_xref="MGI:MGI:1915160"
     exon            1..274
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..3032
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /note="isoform b is encoded by transcript variant 2; run
                     domain Beclin-1 interacting and cystein-rich containing
                     protein; run domain Beclin-1 interacting and cysteine-rich
                     containing protein; run domain Beclin-1-interacting and
                     cysteine-rich domain-containing protein"
                     /codon_start=1
                     /product="run domain Beclin-1-interacting and
                     cysteine-rich domain-containing protein isoform b"
                     /protein_id="NP_766203.1"
                     /db_xref="CCDS:CCDS28124.1"
                     /db_xref="GeneID:100502698"
                     /db_xref="MGI:MGI:1915160"
                     /translation="MRPEGAGMDLGGGDGERLLEKSRREHWQLLGNLKTTVEGLVSAN
                     CPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWLSPHSALHVEK
                     FISLHESDQSDTDSVSERAVAELWLQHSLQCHCLSAQLRPLLGDRQYIRKFYTETAFL
                     LSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKQESPLLVTKSQSLTALPGSTYTP
                     PASYAQHSYFGSSSSLQSMPQSSHSSERRSTSFSLSGPSWQPQEDRECLSPAETQTTP
                     APLPSDSTLAQDSPLTAQEMSDSTLTSPLEASWVSSQNDSPSDVSEGPEYLAIGNPAP
                     HGRTASCESHSSNGESSSSHLFSSSSSQKLESAASSLGDQEEGRQSQAGSVLRRSSFS
                     EGQTAPVASGTKKSHIRSHSDTNIASRGAAEGGQYLCSGEGMFRRPSEGQSLISYLSE
                     QDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQK
                     IRLRRQQIRTKNLLPAYRETENGSFRVTSSSSQFSSRDSTQLSESGSAEDADDLEIQD
                     ADIRRSAVSNGKSSFSQNLSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEH
                     DAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIA
                     VAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMVVPSRILRKWDFSKY
                     YVSNFSKDLLLKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLYHMKNMFKTCRLA
                     KELLDSFDVVPGHLTEDLHLYSLSDLTATKKGELGPRLAELTRAGAAHVERCMLCQAK
                     GFICEFCQNEEDVIFPFELHKCRTCEECKACYHKTCFKSGRCPRCERLQARRELLAKQ
                     SLESYLSDYEEEPTEALALEATVLETT"
     exon            275..428
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            429..512
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            513..672
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            673..779
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            780..939
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1473
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1589
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1800
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1902
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..1960
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2093
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2239
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2304
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2543
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2605
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2759
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2853
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..5298
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5253..5258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /note="hexamer: AATAAA"
     polyA_site      5293
                     /gene="Rubcn"
                     /gene_synonym="1700021K19Rik; 5330403K09; mKIAA0226;
                     Rubicon"
                     /note="major polyA site"
ORIGIN      
        1 gtagttcttg gagccggaag tgggggtggc aggggtagag cggcagctga ggcagcagtc
       61 cttgactctg tcgctgtcag ggtgtagtgc atggttctaa gctcaccagc catggtgcct
      121 cccctctctt gagggtgctc cgggtaccct ggcggtcgaa gcggcctgga gcctctctct
      181 ggccccgaga cggccttata gtccggatgc gtccggaggg cgcgggaatg gatcttggcg
      241 gcggcgatgg ggagcgtctg ctagagaaga gcaggaggga gcactggcag ctgctgggta
      301 atttgaagac gactgtggaa ggtttggtgt cagccaactg ccccaatgtc tggtccaagt
      361 atggtggcct ggagcggctg tgcagggaca tgcagaacat cctgtatcat gggctcatcc
      421 atgaccaggt gtgttgccgc caggctgatt actggcagtt tgtgaaagac attcggtggc
      481 tcagcccgca ctcagccctt cacgtggaga agttcatcag tttgcatgag agcgaccaga
      541 gcgacactga cagcgtgagt gagcgtgctg ttgcagagct gtggctgcag catagcctgc
      601 agtgccactg cctctcagcc cagctccggc ctctgctcgg ggacagacag tacatcagaa
      661 aattctacac agaaactgcc ttcctgctga gtgacgccca cgtcacagcc atgctccagt
      721 gcctggaagc agtggaacag aacaaccccc gtcttctggc tcagatcgat gcatccatgt
      781 ttgccagaaa gcaggagagc ccgctgctgg tcacaaagag ccagagtctg accgctctgc
      841 ctggttccac atacacccct ccagctagct atgctcagca ttcctacttt gggtcctcct
      901 ccagccttca gtctatgcca cagtccagcc acagctcaga gagaagatct acttcctttt
      961 cactctctgg cccttcctgg caacctcaag aagacagaga atgcctctca ccagcagaga
     1021 ctcaaaccac cccagctccg ctgccttcag actctactct agcccaggat tccccactga
     1081 cagcacagga gatgagcgac agcactctga ctagccccct agaggcatcc tgggtcagca
     1141 gccagaatga ctccccaagt gatgtcagtg aggggcccga gtacctggcc attggcaacc
     1201 cagcccctca tggccggact gccagttgtg agagtcacag cagcaacggc gagagcagca
     1261 gctctcactt gttctcctcc agcagctctc aaaagctgga gtctgctgcc tcttctctag
     1321 gggaccaaga ggaaggcagg cagagtcagg caggcagcgt ccttcgcagg tccagcttct
     1381 cagaagggca gacagcccct gtcgccagcg ggacaaagaa gagccatatt cgctcccact
     1441 cggacaccaa cattgcctcc agaggagccg cagagggtgg tcagtacctc tgctcaggag
     1501 aaggcatgtt tagaagacca tcagaaggac agtccctcat cagttacctc tctgagcaag
     1561 actttggcag ctgtgcagac ctggaaaagg aaaacgccca cttcagcatc tccgagtcct
     1621 tgattgccgc cattgagctg atgaagtgca acatgatgag ccagtgtcta gaagaggagg
     1681 aagtagagga ggaagatagt gaccgagaga tccaggaact gaagcagaag atccgccttc
     1741 ggcgccagca gatccgcacc aaaaacctgc tccctgcgta ccgggagact gagaatggaa
     1801 gcttccgggt cacctccagc agctcccagt tcagttcacg ggattcgacg cagctctctg
     1861 agtccggctc tgctgaggat gctgacgact tggaaatcca agacgctgac atccggagga
     1921 gtgcagtctc aaacggcaaa tcatccttct cccagaatct ctcgcactgc ttcctgcact
     1981 ccacatcggc tgaggcagtg gccatggggc ttctgaagca gtttgagggg atgcagcttc
     2041 cagctgcctc ggagctcgag tggctagtcc cagagcatga tgctccacag aagctcctac
     2101 ccatccccga ctccctgccc atctcaccag atgacgggca gcacgcggac atctacaagc
     2161 tgcgaatccg cgtccgtggc aacctggagt gggctccgcc ccggccgcag atcattttta
     2221 atgttcatcc agcccccacg aggaagattg ccgtggccaa gcagaattac cgctgtgcgg
     2281 gatgtggcat ccggacggac cccgactata tcaagcggct gcggtactgt gagtacctag
     2341 gcaagtactt ctgccagtgc tgccacgaga acgcccagat ggtcgtcccc agccgcattc
     2401 tgcgcaaatg ggacttcagc aagtactatg tcagcaactt ctccaaggac ctgctcctga
     2461 agatctggaa tgatcctctc ttcaatgtgc aggacatcaa tagcgcactc tacaggaagg
     2521 tcaagctgct taaccaagtc cggctgctgc gggttcagct gtaccacatg aagaacatgt
     2581 ttaagacatg ccgactggcc aaagagctcc tggattcctt tgacgtggtt ccaggccacc
     2641 tgacagagga cctccatctg tactcgctga gtgacctgac tgcaaccaag aagggagaac
     2701 tggggccccg gctggcggag ctcactagag cgggagccgc ccacgtggag agatgcatgc
     2761 tgtgtcaggc caagggcttc atctgtgaat tctgtcagaa tgaggaggat gtcatctttc
     2821 cttttgagct gcataagtgc cggacttgtg aagagtgtaa agcgtgttac cataaaactt
     2881 gcttcaagtc tggacgctgc ccccggtgtg agcggctgca ggcccgacgg gagctgctgg
     2941 ccaagcagag cctggagtcc tacctgtctg actatgagga ggagcccaca gaagccttgg
     3001 ctctagaggc caccgtccta gagaccacct gaggaatgca ctaagccctt tgtgtaaggg
     3061 tctggcgagg gtcagatata gactgagcca caccttcaag gaaggtgggg ccttttaaat
     3121 atatatatat tgtcattttt tatgacttgt ctgctgtcca agtgtaggca acctttcttg
     3181 gttgatggat ccagtctctt gtgacagctg tctgtgggca ctgggtattt ttgtcagggg
     3241 aatggacatc gagacccagt tctgacccgc tcggcctctt ctactaggac cagatctggt
     3301 tacagctgca gctaacgatc atacccactg ttgacttagt tcctcagagt ctgagccagg
     3361 cctctctcag ccagcgacct tctcctttgc tgctgaagca ggagaatggt cttttcttag
     3421 gcctagtcct aacagagact aagactgtgc cctgagtcac gggagagaag gggggtatac
     3481 aacgggcatt ctgtctggcc acttaggtat acgggggggc agggcaggta ggaacagaac
     3541 catccttgtt ctagcatctt cgggatctca aagggcaacc atgacttccc tgtttcaaat
     3601 attcctggca taggtgggca caagctggtt tgtgtactct ggtgggcttg gatcaaagac
     3661 cagatccgag tcttaggtca ggccacaagc ctataagtgc ggctacagct ggtgcttaag
     3721 gggcggcttt ttcagatagg catctcctcc ggggtggtca gtctctcagt taactatcgt
     3781 gagtgcaggt gtgcttcatg ctcaggcaag tccctgatgc tacctaaggc gaggatagaa
     3841 acccctccca ccatctgaat agtgggttcc ttactgctct gtacgactat gtggtcattg
     3901 tggttccagt actgatatga cccaactcca ttctctgtcc ccttcctcca gagagctgca
     3961 gcccttcctc ttggctgatc tcttgcctta gtttcttttc tcagagttgg gggaagccag
     4021 ccaactagta gcactaagta aagcacttgg cagtttgtga atgtgtctgt ttgctcagag
     4081 taacaggacc tttatgcagt taactaactc ttcagctttg taatctatgt caaaccatcc
     4141 atttcatggt actaatttta atagactctt cccctatcac ctaatgtgca ttttttgtgg
     4201 gaattgccct ggactaggct gcacatgatg gtggtggtgt ttgagttgct gcagcaccga
     4261 cctcggtaaa cactggtcag ggctgcctct cagagcctgg cagttctgtg aacttaatcc
     4321 acagccctct tgttctggag ctggcctgtg gcttcctgta cttgaccgct agtaaaatca
     4381 ttggtccagg cagtgtcgca agccgtcaaa tggagttgca tgttgttgat ggaagctgac
     4441 aggtagctgt gcttagaggg aacgagggat ctcccagaga ggcagaaggg gcatgacata
     4501 acagccaagc tgagctccag cagagcaaga gggagcatgc caggctagtt ccaaggtgtg
     4561 gctcctctgc ctcacacttg ggatctgaca actacatgga ctgggactgc tctcagtagt
     4621 cgatgaggca gaaagccgag gccagagcca gttctgcaga gctggttaga aatccctccc
     4681 aattcactta tcactgagtc agacctcaga gacagactct gactccaggt ccagttcctg
     4741 gctgacttcc gtccagctgc tcagtgaagt caccacactg gaggagactc cacccatgct
     4801 cccagtctac aaaggctcaa gcagcctcac tgacgtccct gttcctttcc acctccccac
     4861 cccaccccat ccctctaggt cacatacctt ctagagctcc tgagtccgct gtcattactc
     4921 accaaatacc catgtctgtt tatttgagga gccggagcag ggggtgtggt gaaagcactt
     4981 tgtcacttac agtatctgag gatcccctgc tacactgatg ggaggtgggg atggagctta
     5041 gggaggggag cagcactctg tacagagggg acagctccaa gtgctctgca cttaaggggc
     5101 ccttcgtcct ctgctgaggg ctggagagct tttgccgaag agaaaaggag caagagtcta
     5161 aatgaaaacg ctgtttgtgt acaacctgtg tttagaactg gattctcacc tgctgtaatt
     5221 tacgcacatc ctgacagtcc atggtcatct ttaataaact aaggaaaata aatcttgcct
     5281 tttatttacc tgccttgaaa aaaaaaaaaa aaaaaa
//
