LOCUS       NM_001359319            3957 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus rhotekin 2 (Rtkn2), transcript variant 2, mRNA.
ACCESSION   NM_001359319 XM_006513291
VERSION     NM_001359319.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Hutchison,V., Lynch,A., Gutierrez-Gamez,A.M. and Chen,J.
  TITLE     Inducible tricolor reporter mouse for parallel imaging of
            lysosomes, mitochondria, and microtubules
  JOURNAL   J Cell Biol 223 (1) (2024)
   PUBMED   37917008
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Little,D.R., Lynch,A.M., Yan,Y., Akiyama,H., Kimura,S. and Chen,J.
  TITLE     Differential chromatin binding of the lung lineage transcription
            factor NKX2-1 resolves opposing murine alveolar cell fates in vivo
  JOURNAL   Nat Commun 12 (1), 2509 (2021)
   PUBMED   33947861
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3957)
  AUTHORS   Li,C., Smith,S.M., Peinado,N., Gao,F., Li,W., Lee,M.K., Zhou,B.,
            Bellusci,S., Pryhuber,G.S., Ho,H.H., Borok,Z. and Minoo,P.
  TITLE     WNT5a-ROR Signaling Is Essential for Alveologenesis
  JOURNAL   Cells 9 (2), 384 (2020)
   PUBMED   32046118
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3957)
  AUTHORS   Nicod,J., Davies,R.W., Cai,N., Hassett,C., Goodstadt,L.,
            Cosgrove,C., Yee,B.K., Lionikaite,V., McIntyre,R.E., Remme,C.A.,
            Lodder,E.M., Gregory,J.S., Hough,T., Joynson,R., Phelps,H.,
            Nell,B., Rowe,C., Wood,J., Walling,A., Bopp,N., Bhomra,A.,
            Hernandez-Pliego,P., Callebert,J., Aspden,R.M., Talbot,N.P.,
            Robbins,P.A., Harrison,M., Fray,M., Launay,J.M., Pinto,Y.M.,
            Blizard,D.A., Bezzina,C.R., Adams,D.J., Franken,P., Weaver,T.,
            Wells,S., Brown,S.D., Potter,P.K., Klenerman,P., Lionikas,A.,
            Mott,R. and Flint,J.
  TITLE     Genome-wide association of multiple complex traits in outbred mice
            by ultra-low-coverage sequencing
  JOURNAL   Nat Genet 48 (8), 912-918 (2016)
   PUBMED   27376238
REFERENCE   5  (bases 1 to 3957)
  AUTHORS   Tulin,E.E., Onoda,N., Hasegawa,M., Nosaka,T., Nomura,H. and
            Kitamura,T.
  TITLE     Genetic approach and phenotype-based complementation screening for
            identification of stroma cell-derived proteins involved in cell
            proliferation
  JOURNAL   Exp Cell Res 272 (1), 23-31 (2002)
   PUBMED   11740862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK052662.1, AK030986.1, AK045134.1, AC160409.6, AV026520.1 and
            AK087239.1.
            
            On Jan 3, 2018 this sequence version replaced XM_006513291.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030986.1, SRR7652917.1143467.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AK052662.1         2-195
            195-1417            AK030986.1         51-1273
            1418-1418           AK045134.1         1291-1291
            1419-1873           AK030986.1         1275-1729
            1874-1874           AC160409.6         156161-156161
            1875-2391           AK030986.1         1731-2247
            2392-2607           AV026520.1         197-412
            2608-3937           AC160409.6         156895-158224
            3938-3957           AK087239.1         2826-2845
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 35.06 cM"
     gene            1..3957
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /note="rhotekin 2"
                     /db_xref="GeneID:170799"
                     /db_xref="MGI:MGI:2158417"
     exon            1..243
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            244..440
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            441..499
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            500..553
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            554..671
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            672..866
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            867..961
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1068
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1200
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1363
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1471
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..3957
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2582..2587
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /note="hexamer: ATTAAA"
     polyA_site      2609
                     /gene="Rtkn2"
                     /gene_synonym="B130039D23Rik; Mbf; Plekhk1"
                     /note="major polyA site"
ORIGIN      
        1 gcttaagccc tgcggtagca gagggtctgt tcagagtctc gggtctccgt gctgttttaa
       61 agacagagag gctcctagta cgcttgcgcg gcgtcgtggg ccgggttccc ctgcagacca
      121 gacttggtac ggccgagcgg ttggtgcaat ctgcagctgc agccctgggg cgcgcttttc
      181 aaatcggccg tgccttgcac cgagatggag gggcagctgc tgaggggtct cgccgcgcag
      241 caggactgca gcatccgaga gaaaatagac ttagagatcc gcatgcgaga aggaatatgg
      301 aagctgctct cgctgagtac gaaaaaagac caagttttgc atgcagttaa gaacctcatg
      361 gtgtgcagtg ctcggatcca ggcgtacacg gcagagctgc agaagtcaaa agaagagatt
      421 gcaaatcaaa ccggagcacg ggattcgagt tctgaaaata aagaaggaga atcctgcaga
      481 ggaaagatcg ccctgtcaga tatccgaata ccattaatgt ggaaagactc cgatcacttc
      541 agtaataaag aatgcacaca gcgcttcgcc atcttttgct tattcaggat gggagctcag
      601 gtgtttgaca cggacatggt gattgtggac cagacagtca cagatatatg tttcgaaaat
      661 gtcaccatct tcaacgaggc aggaccggac ttccagataa agatagaagt ctacagctgc
      721 agcgcagagg agtcctccct aaccaacacc ccgaggaagc tggctaagaa gctgaagacg
      781 tccatcagca aagccacggg aaggaaaatc agcgcagcgc tccaggaaga gagcccagag
      841 gcgtgcttgc tcgctggttc tgtggcaggt gcaaagtacc atttgctggc tcataccacc
      901 ctgaccttgg aaaatgctgg agactgcttt aagacccata acctgtctgt tcacggagat
      961 gaggagtgtt ccttttggct tcccttgtat ggcaacgtgt gctgccggct ggtggcccag
     1021 ccagcttgca tggctgcaga tgcatttgca ggattcctta atgaacagca aacaggaaaa
     1081 ggtctggttg gctggagaag gctgtactgt gctctgagag ggggtaaact ccgctgcttt
     1141 tatggtccgg aagaaattga agctaaagtg gagccggctc tggtggttcc tattgacaag
     1201 gaaaccagaa ttcaggccgt ggaaaaggat tcaaagaaaa tgcactgttt ctctgtcctc
     1261 agcacagccg ctgggcgggc ggtgagtcac atctttgcgg ctgacagcct agcagacttt
     1321 caggagtgga tgggcgcctt ccggcaacat ttctttgatc tcagccaatg gaagcattgt
     1381 tgtgaagaac ttatgagaat tgagattatg tcaccacgaa aaccaccttt gttcctggca
     1441 aaggaggcga cctcagtgta ttatgatatg agcatcgatt cacccgtgaa actggaaagt
     1501 gtaacagaca taatacaaaa gaaaatcgga gagaccaacc gacagttcct cattggtcgg
     1561 gatgaccaat ccgcagcacc cccgtgggcc gctgtctttg atggcaatca cgaaatggtc
     1621 atagagaaga aagtgttgtc cccaataggc gaaccggctc cggatggaaa aagaaaaaag
     1681 aggcgagccc cactccctcc taccgatcag ccgcccttct gcataaaaac ccagggtaga
     1741 gcgaatcagt ccaaggacag cgcaacccag gctggggtct ctggagcctc atcttcgcct
     1801 tcggatccca gactgtcgcc gcctacgcat catctccaga aacctgtggc agcccctcgc
     1861 aaacttctcc ctgccaggaa gaacagttca gcggacattg ggcacacgga cactaaaacc
     1921 agccttgatg cgaagccagt gccagttcca aggcagaaat ccatcagaga cattctggac
     1981 ccacgatctt ggctccaagc acaagtatag gaagccggga ctatgtaaat ccagctgtaa
     2041 ggctctggtc agaggcattc tatgggtaat gttatgtaat tgtataggta aaggccagag
     2101 cagtttccag aagccattaa taagctgtta ggtacagaaa agcaccttta agtagagaat
     2161 tagatttctc ctttccagaa tagaaatgat cctcccctcc ccggagcaaa agaggcagtt
     2221 tctagacagt agcctacttt ttggaagggt taatttactc ctaatttagt agcaggcagt
     2281 gaacagacag aaggcagagg aaagcattgt ttttttgttt tttgtttttt ttttttgaga
     2341 gtgagtttag taatgtagaa tgtgttcaat acaagccgta ttctcattgt gagaattgta
     2401 atacttaata gaatatttca gagtttttgt ttaaaccaca aattacagaa tccacccaag
     2461 aaaatgtttc ccctccgctt cctggaattg gcatctaagg gtcctctggc ttctcctctg
     2521 ttgtttaact caatggtctc tagggttgat cttttcaatt cctggtataa agtcttcgaa
     2581 tattaaaaac tctttgtctt taaagggaat ttgtaatctg ctattcaaat tcatttcctt
     2641 gatgagccct gaaatcctgt ggacttggct gctcatcagc aaggagcatg cttcttggtg
     2701 agtgaagtgg caatgagatg tccccccatt gctgggcttc ctgtatgatg ttaatctcca
     2761 tcatggcctc tggagttcag ggagacaagt agccaattaa tgcctttaaa tttttactta
     2821 atacttattt taaaagcaga tggcagattc aaaatttatc aggaaggttt gaccgcagta
     2881 attttcagaa ttaatccttt ttcaattttt taattagatt ttcttttttt tgagtgaggg
     2941 ctgatgtcgg ttattgatat tgcccgttaa aagtcatatt cattcagtaa tcacattggc
     3001 gtcatttcca tagaaggtaa tttccatggc aactaatcac agtgacccaa acaggattat
     3061 aaattgaata ggggggataa ctagcaagag aatgaatatc ttaagtggac aaaaagaaaa
     3121 acccactttt tataataacc ccttttgtaa acaagaacag cggaacggtt ttaaaatgtc
     3181 atcataattt ccaagcaaaa cttcccagag ggtattaaaa cagtcctgaa cagaccagga
     3241 cctcccaagc cctaacgggc accacaaacc atcacactga aggaaggagg ctccaggctg
     3301 ctgagctctg tccatctgga tgggaactac atctgtgtaa agtggccaac aaaggtattg
     3361 atcggaccaa cccattcatc gcttttaggg gatagtttgt ctgttttgtc ttgtttttgt
     3421 ttctcatttc tgcacagtgc tcctctaact aacaggtaat atgtacacta ttgcttgccc
     3481 aggaccccca gtaaaaaagg ggaacccgag agcagcgaca aagggccctc tgtgaaggca
     3541 gaaggcagaa gctgtgtgga gactcctaca gccatggaaa cttgaagaat aggtggccgt
     3601 ggttttgttt ttgttttaat ctaacagggt ctcaaaagtt ttggtggtaa ttgccatttc
     3661 tttcttttta atacttactt tatgtattta agtacaccat agctgtcttc agacacacca
     3721 gaagagggca tcagatccca ttacagatgg tcatgagcca ccatgtggtt gttgggaact
     3781 gaactcagca cctctggaag agcaagtcct gagccatctc tccagcccca gtaaagttgt
     3841 attttttgct tgaaaatgcc tctgttagcc ctcaccgata tcttcaacaa tgcccatgaa
     3901 cttttgatgc aacctctttt tttttttttt tttttttaat taaaagatgc tgctggc
//
