LOCUS       NM_001136227            2153 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus rhotekin (Rtkn), transcript variant 1, mRNA.
ACCESSION   NM_001136227
VERSION     NM_001136227.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Yuasa,K., Nagame,T., Dohi,M., Yanagita,Y., Yamagami,S., Nagahama,M.
            and Tsuji,A.
  TITLE     cGMP-dependent protein kinase I is involved in neurite outgrowth
            via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells
  JOURNAL   Biochem Biophys Res Commun 421 (2), 239-244 (2012)
   PUBMED   22503686
  REMARK    GeneRIF: these findings suggest that cGK-Ialpha interacts with and
            phosphorylates rhotekin, thereby contributing to neurite outgrowth
            regulation.
REFERENCE   3  (bases 1 to 2153)
  AUTHORS   Zhou,C., Wong,O.G., Masters,J.R. and Williamson,M.
  TITLE     Effect of cancer-associated mutations in the PlexinB1 gene
  JOURNAL   Mol Cancer 11, 11 (2012)
   PUBMED   22404908
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2153)
  AUTHORS   Margolis,S.S., Salogiannis,J., Lipton,D.M., Mandel-Brehm,C.,
            Wills,Z.P., Mardinly,A.R., Hu,L., Greer,P.L., Bikoff,J.B., Ho,H.Y.,
            Soskis,M.J., Sahin,M. and Greenberg,M.E.
  TITLE     EphB-mediated degradation of the RhoA GEF Ephexin5 relieves a
            developmental brake on excitatory synapse formation
  JOURNAL   Cell 143 (3), 442-455 (2010)
   PUBMED   21029865
REFERENCE   5  (bases 1 to 2153)
  AUTHORS   Rosario,C.O., Ko,M.A., Haffani,Y.Z., Gladdy,R.A., Paderova,J.,
            Pollett,A., Squire,J.A., Dennis,J.W. and Swallow,C.J.
  TITLE     Plk4 is required for cytokinesis and maintenance of chromosomal
            stability
  JOURNAL   Proc Natl Acad Sci U S A 107 (15), 6888-6893 (2010)
   PUBMED   20348415
REFERENCE   6  (bases 1 to 2153)
  AUTHORS   Reynaud,C., Fabre,S. and Jalinot,P.
  TITLE     The PDZ protein TIP-1 interacts with the Rho effector rhotekin and
            is involved in Rho signaling to the serum response element
  JOURNAL   J Biol Chem 275 (43), 33962-33968 (2000)
   PUBMED   10940294
REFERENCE   7  (bases 1 to 2153)
  AUTHORS   Fu,Q., Yu,L., Liu,Q., Zhang,J., Zhang,H. and Zhao,S.
  TITLE     Molecular cloning, expression characterization, and mapping of a
            novel putative inhibitor of rho GTPase activity, RTKN, to
            D2S145-D2S286
  JOURNAL   Genomics 66 (3), 328-332 (2000)
   PUBMED   10873388
REFERENCE   8  (bases 1 to 2153)
  AUTHORS   Weber,J.S., Jang,W., Simin,K., Lu,W., Yu,J. and Meisler,M.H.
  TITLE     High-resolution genetic, physical, and transcript map of the mnd2
            region of mouse chromosome 6
  JOURNAL   Genomics 54 (1), 107-115 (1998)
   PUBMED   9806835
REFERENCE   9  (bases 1 to 2153)
  AUTHORS   Jang,W., Weber,J.S., Harkins,E.B. and Meisler,M.H.
  TITLE     Localization of the rhotekin gene RTKN on the physical maps of
            mouse chromosome 6 and human chromosome 2p13 and exclusion as a
            candidate for mnd2 and LGMD2B
  JOURNAL   Genomics 40 (3), 506-507 (1997)
   PUBMED   9073523
REFERENCE   10 (bases 1 to 2153)
  AUTHORS   Reid,T., Furuyashiki,T., Ishizaki,T., Watanabe,G., Watanabe,N.,
            Fujisawa,K., Morii,N., Madaule,P. and Narumiya,S.
  TITLE     Rhotekin, a new putative target for Rho bearing homology to a
            serine/threonine kinase, PKN, and rhophilin in the rho-binding
            domain
  JOURNAL   J Biol Chem 271 (23), 13556-13560 (1996)
   PUBMED   8662891
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104324.21.
            
            On Aug 4, 2022 this sequence version replaced NM_001136227.1.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076155.1, SRR17784645.873977.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-210               AC104324.21        172391-172600
            211-410             AC104324.21        182472-182671
            411-472             AC104324.21        184030-184091
            473-526             AC104324.21        184193-184246
            527-644             AC104324.21        184369-184486
            645-854             AC104324.21        184647-184856
            855-949             AC104324.21        186005-186099
            950-1056            AC104324.21        186213-186319
            1057-1185           AC104324.21        186513-186641
            1186-1354           AC104324.21        187449-187617
            1355-1459           AC104324.21        187778-187882
            1460-2153           AC104324.21        188427-189120
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.94 cM"
     gene            1..2153
                     /gene="Rtkn"
                     /note="rhotekin"
                     /db_xref="GeneID:20166"
                     /db_xref="MGI:MGI:107371"
     exon            1..210
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..1794
                     /gene="Rtkn"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="rhotekin isoform a"
                     /protein_id="NP_001129699.1"
                     /db_xref="CCDS:CCDS51817.1"
                     /db_xref="GeneID:20166"
                     /db_xref="MGI:MGI:107371"
                     /translation="MFSRNHRSRITVARGSALEMEFKRGRFRLSFFSESPEDTELQRK
                     LDHEIRMRDGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLEKTG
                     RRPSDSVQPAQHSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQIGEQIQ
                     DTEMVLVDRTLTDISFQNNVLFAEAEPDFELRLELYGACVEEEGALAGAPKRLATKLS
                     SSLGRSSGKRVRASLDSAGASGNSPVLLPTPAVGGPRFHLLAHTTLTLEEVQDGFRTH
                     DLTLTSHEENPAWLPLYGSVCCRLAAQPLCMIQPTASGALRVQQAGELQNGTLVHGVL
                     KGTNLFCYWRSEDADTGQEPLFTIVINKETRVRAGELEQAPEWPFTLSISNKYGDDEV
                     TNTLQLESREALQNWMEALWQLFFDMSQWRHCCDEVMKIETPAPRKPPQALAKQGSLY
                     HEMAIEPLDDIAAVTDILAQREGTRLEPSPPWLAMFTDQPALPSSCSPASVAPVPTWM
                     QPLPWGRPRTFSLDAAPADHSLGPSRSVAPLPPQRSPKSRGFYSKSQLGPWLQSPV"
     exon            211..410
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            411..472
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            473..526
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            527..644
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            645..854
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            855..949
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1056
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1185
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1354
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1459
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..2153
                     /gene="Rtkn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rtkn"
                     /note="hexamer: ATTAAA"
     polyA_site      2153
                     /gene="Rtkn"
                     /note="major polyA site"
ORIGIN      
        1 attcccggct gtactggtag gagggcggcc gtcgagggac cggagcccaa gcccgcgaga
       61 gactgatccc aggagcgtac tgggcaaggg gctgccagca tgttctctcg aaaccatcgg
      121 agtcggatca cagtggccag gggctccgcc ctggagatgg agttcaaacg cggccgcttc
      181 cgacttagtt tcttcagcga gtcgccggag gacacagagc tgcagaggaa actagatcat
      241 gagatccgga tgagggatgg ggcctgcaag ctgctggcag cctgctccca gcgagagcag
      301 gctctggaag ccaccaagag cctgctggtg tgcaacagcc gtattctcag ctacatgggt
      361 gagctgcagc ggcgaaagga ggcccaggtg ctggagaaga caggcaggcg accttcggac
      421 agtgtgcagc ctgctcagca ctccccttgc cgtggccggg tctgcatctc tgacctccgg
      481 atcccactca tgtggaagga tacagagtat ttcaagaaca aaggcgacct gcaccgctgg
      541 gctgtgttcc tgctgctgca gattggggaa cagattcagg acacagagat ggtcctggta
      601 gacaggaccc tcacagacat ctcctttcag aataatgtgc tttttgctga ggcagagcct
      661 gactttgaac tgcggctgga gctgtatggg gcctgtgtgg aagaggaggg agccctggct
      721 ggtgctccca agaggcttgc caccaaactc agtagctccc tgggccgttc ctcggggaag
      781 agagtcaggg catcgctgga cagtgctggg gcctccggga acagtcccgt cctgttacct
      841 accccagctg tgggaggtcc tcgattccac ctcttggccc acaccactct taccctagaa
      901 gaagtgcaag atggattccg tacacatgac ctcacgctca ccagtcatga ggagaaccct
      961 gcctggctac ccctttatgg cagcgtgtgt tgccgcctgg cagctcagcc tctctgcatg
     1021 atccagccca ctgcaagtgg tgcccttagg gtgcagcaag ccggggagct gcagaatggg
     1081 acactggtac atggagtcct gaaaggcaca aacctctttt gttactggag atctgaggat
     1141 gcagacactg ggcaagaacc actgttcact atcgtcatca acaaggagac tagggtccgg
     1201 gcaggtgagt tggagcaggc cccagagtgg cctttcaccc tcagcatcag caacaagtat
     1261 ggggatgatg aggtgacaaa caccctccag ctggagagca gagaagccct gcagaactgg
     1321 atggaggcgc tttggcagct cttctttgac atgagccagt ggaggcactg ttgtgatgaa
     1381 gtcatgaaga tcgaaactcc tgcgccccgg aaaccacccc aagccctggc caagcagggg
     1441 tccttatacc acgagatggc cattgagccg ctagacgaca tcgcagctgt gacagacatc
     1501 ctggcccagc gggagggcac aaggctggag ccatccccac cctggctagc aatgtttaca
     1561 gaccagcctg ccttgcctag ctcctgctcg cctgcctcag tggccccagt cccaacctgg
     1621 atgcaacccc tgccctgggg aagaccccga accttttccc tggatgctgc ccctgcagac
     1681 cactcccttg ggccttctcg ctcggttgca ccccttcccc cgcagagatc ccccaaatcc
     1741 agaggtttct acagcaaaag ccagctcggc ccatggctcc agtccccagt gtgagaggaa
     1801 ccagcaacag gatctggcca agaaagaaaa tgacccaagg acaactgggc tctaggtcca
     1861 gcactgtggg gcaccagttg gccagcctgg gctcctcttc ctccactgga ctaggacgga
     1921 aggctgggga ggggtaatgg aaacccctct taagttgtgg ctatcatggt acagaggggg
     1981 accattcctg gagctggcta ggattcccct aagcgcttcc tgggagaaaa ctggaacaac
     2041 tctgccctat ctgcctgcac taaccagcct ccagggcggc aacggagagt ggagaggaaa
     2101 gcacctcccc ccgtgatccc aatatcaacc attaaagtta tttaacagct cga
//
