LOCUS       NM_030112               4680 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1),
            mRNA.
ACCESSION   NM_030112 XM_283757 XM_917502
VERSION     NM_030112.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Langenbacher,A.D., Lu,F., Crisman,L., Huang,Z.Y.S., Chapski,D.J.,
            Vondriska,T.M., Wang,Y., Gao,C. and Chen,J.N.
  TITLE     Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte
            Biology
  JOURNAL   J Cardiovasc Dev Dis 10 (5), 221 (2023)
   PUBMED   37233188
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4680)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4680)
  AUTHORS   Strikoudis,A., Lazaris,C., Trimarchi,T., Galvao Neto,A.L., Yang,Y.,
            Ntziachristos,P., Rothbart,S., Buckley,S., Dolgalev,I.,
            Stadtfeld,M., Strahl,B.D., Dynlacht,B.D., Tsirigos,A. and
            Aifantis,I.
  TITLE     Regulation of transcriptional elongation in pluripotency and cell
            differentiation by the PHD-finger protein Phf5a
  JOURNAL   Nat Cell Biol 18 (11), 1127-1138 (2016)
   PUBMED   27749823
REFERENCE   5  (bases 1 to 4680)
  AUTHORS   Zhang,K., Haversat,J.M. and Mager,J.
  TITLE     CTR9/PAF1c regulates molecular lineage identity, histone H3K36
            trimethylation and genomic imprinting during preimplantation
            development
  JOURNAL   Dev Biol 383 (1), 15-27 (2013)
   PUBMED   24036311
REFERENCE   6  (bases 1 to 4680)
  AUTHORS   Ding,L., Paszkowski-Rogacz,M., Nitzsche,A., Slabicki,M.M.,
            Heninger,A.K., de Vries,I., Kittler,R., Junqueira,M.,
            Shevchenko,A., Schulz,H., Hubner,N., Doss,M.X., Sachinidis,A.,
            Hescheler,J., Iacone,R., Anastassiadis,K., Stewart,A.F.,
            Pisabarro,M.T., Caldarelli,A., Poser,I., Theis,M. and Buchholz,F.
  TITLE     A genome-scale RNAi screen for Oct4 modulators defines a role of
            the Paf1 complex for embryonic stem cell identity
  JOURNAL   Cell Stem Cell 4 (5), 403-415 (2009)
   PUBMED   19345177
REFERENCE   7  (bases 1 to 4680)
  AUTHORS   de Jong,R.N., Truffault,V., Diercks,T., Ab,E., Daniels,M.A.,
            Kaptein,R. and Folkers,G.E.
  TITLE     Structure and DNA binding of the human Rtf1 Plus3 domain
  JOURNAL   Structure 16 (1), 149-159 (2008)
   PUBMED   18184592
  REMARK    GeneRIF: Potential role of Rtf1 Plus single stranded DNA binding
            during transcription elongation.
REFERENCE   8  (bases 1 to 4680)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 4680)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4680)
  AUTHORS   Tate,P., Lee,M., Tweedie,S., Skarnes,W.C. and Bickmore,W.A.
  TITLE     Capturing novel mouse genes encoding chromosomal and other nuclear
            proteins
  JOURNAL   J Cell Sci 111 (Pt 17), 2575-2585 (1998)
   PUBMED   9701556
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844536.13, BY270385.1, AK019305.1, BC117849.1, CN839001.1,
            CN532066.1 and AW491091.1.
            
            On Aug 12, 2008 this sequence version replaced NM_030112.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC117849.1, SRR7345562.1163660.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL844536.13        136706-136711
            7-425               BY270385.1         1-419
            426-708             AK019305.1         171-453
            709-1355            BC117849.1         561-1207
            1356-1587           CN839001.1         119-350
            1588-2196           CN532066.1         104-712
            2197-2376           BC117849.1         2049-2228
            2377-4396           AL844536.13        194758-196777
            4397-4680           AW491091.1         1-284               c
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.97 cM"
     gene            1..4680
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="RTF1, Paf1/RNA polymerase II complex component"
                     /db_xref="GeneID:76246"
                     /db_xref="MGI:MGI:1309480"
     CDS             1..2148
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="gene trap locus 7; Rtf1, Paf1/RNA polymerase II
                     complex component, homolog"
                     /codon_start=1
                     /product="RNA polymerase-associated protein RTF1 homolog"
                     /protein_id="NP_084388.2"
                     /db_xref="CCDS:CCDS50673.1"
                     /db_xref="GeneID:76246"
                     /db_xref="MGI:MGI:1309480"
                     /translation="MRGRLCVGRAAAVAAAVAAAAVAVPLAGGQEGSQGGVRRGSRGT
                     TMVKKRKGRVVIDSDTEDSGSDENLDQELLSLAKRKRSDSEEKEPPVSQPAASSDSET
                     SDSDDEWTFGSNKNKKKGKTRKVEKKGAMKKQANKAASSGSSDRDSSAESSAPEEGEV
                     SDSESSSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIE
                     KREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKR
                     DEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEDDDKSSEKSD
                     RSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFV
                     RIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQ
                     EFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFR
                     KAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTKNISAIS
                     YINQRNREWNIVESEKALVAESHNMRNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQ
                     LNAKYGSGVLPDAPKEMSKGQGKDKDLNSKTASDLSEDLFKVHDFDVKIDLQVPSSES
                     KALAITSKAPPAKDGAPRRSLNLEDYKKRRGLI"
     exon            1..213
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            214..324
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            325..472
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            473..677
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            678..792
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            793..904
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1040
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1218
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1301
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1389
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1497
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1575
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1697
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1755
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1833
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1929
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2041
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..4664
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2902..2907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="hexamer: ATTAAA"
     polyA_site      2926
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="major polyA site"
     regulatory      4108..4113
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="hexamer: AATAAA"
     polyA_site      4129
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="major polyA site"
     regulatory      4635..4640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
                     /note="hexamer: AATAAA"
     polyA_site      4661
                     /gene="Rtf1"
                     /gene_synonym="2900005O08Rik; 6530416A09Rik; cbp82; Gtl7"
ORIGIN      
        1 atgcgcggtc gcctttgtgt gggtcgagcg gcggcggtgg cggcggcggt ggcggcggcg
       61 gcagtggcgg tcccactggc aggcgggcaa gaggggagtc agggcggcgt ccggcgtgga
      121 agccggggga ccaccatggt aaagaagcgg aaaggccgtg tcgtgatcga ctcggatacg
      181 gaagacagcg gcagtgacga gaacctggat caggagctct tgtccctggc gaaacggaag
      241 cgcagtgact ccgaggagaa ggagcctcct gtgagtcagc ctgcagcctc atcagattca
      301 gagacctccg acagtgatga tgagtggaca tttgggagca ataaaaataa gaagaaagga
      361 aaaaccagaa aagtagagaa gaaaggagcc atgaagaagc aagccaacaa agctgcctcc
      421 tcgggcagct cagacagaga cagctcagct gagagctctg cccctgagga aggtgaagtg
      481 tcagattctg agagcagcag ctcctcttcc agttcagatt cagactcctc gtcagaagat
      541 gaagaattcc atgatggcta tggagaggat cttatgggag atgaggaaga tagggcccga
      601 ctggagcaga tgacggagaa ggagagggag caggagctct tcaaccgcat agagaagaga
      661 gaggtgttga aaagaagatt tgaaattaag aaaaaactaa aaacagccaa aaagaaagag
      721 aagaaagaaa aaaagaaaaa gcaggaagaa gaacaagaaa agaagaagct gacacagatt
      781 caggaatctc aggttacttc ccacaataaa gaacggcgtt ccaagcggga tgaaaaacta
      841 gataagaaat ctcaagccat ggaggagcta aaagcagagc gagaaaaacg gaagaacaga
      901 acagctgagc tcctcgccaa aaaacagcca ttgaaaacca gtgaggtcta ctctgacgat
      961 gaggaggagg aagatgatga caagtccagt gagaaatcgg accgctcatc ccggacatca
     1021 tcatctgatg aagaggaaga gaaagaagaa atccctccaa aatcacagcc agtctcatta
     1081 cccgaggaac tgaatcgggt tcggttatca cggcataagc tagagcgctg gtgtcacatg
     1141 cccttctttg ctaaaactgt cacaggatgt tttgtacgga ttggcattgg aaaccacaat
     1201 agcaagccag tttaccgggt ggctgagatc acgggtgttg tggaaactgc caaagtttac
     1261 cagctgggtg gcaccagaac aaataaaggg ctccagctac ggcatggcaa tgaccagcga
     1321 gtgttccgcc tagagtttgt gtccaaccaa gaattcactg aaagtgaatt catgaagtgg
     1381 aaagaagcga tgttttctgc tggcatgcag ttgcccacac tagatgaaat caataaaaag
     1441 gaattatcta ttaaggaagc tctcaattat aaattcaatg atcaggacat tgaggagatt
     1501 gtaaaagaga aggaaaggtt cagaaaagcc ccaccaaact atgctatgaa gaagactcag
     1561 ctgctaaagg agaaggctat ggctgaggac ctgggagatc aggacaaagc caaacaaatc
     1621 caagaccagc taaatgagct agaggagcga gcagaggccc tggaccgcca gcggaccaag
     1681 aacatatctg ctatcagcta catcaaccag cggaatcggg agtggaatat tgtggagtct
     1741 gagaaggctc tggtggctga aagtcacaac atgagaaacc aacagatgga ccccttcacc
     1801 aggcggcagt gcaaacccac catcgtctct aattccaggg atccagctgt tcaggctgcc
     1861 attttggccc agctcaatgc aaaatacggt tctggggtat taccagatgc tccaaaggaa
     1921 atgagcaagg gtcaaggaaa ggataaagat ttgaattcta agacagccag tgacctctcg
     1981 gaagacctgt tcaaagtgca tgactttgac gtgaagattg acttacaggt tccaagctca
     2041 gagtcaaaag ctttggccat tacctccaaa gccccaccag ccaaggacgg agctccgagg
     2101 agatctctga acttagaaga ctacaaaaaa cgacgggggc ttatttgagc acttccagcc
     2161 tgctgcttcc aaccctgcat gccccgccat cgtcctgcct ttcctctttt cctctgattc
     2221 gccctctttg ggctggagca gcagtggaac tgggaagaga ctctaaactg ccagtcattg
     2281 taatataaac catttactgt atagaccttc cttgtctgtc ccatctccca gcaaccccag
     2341 gccccaccca cggtggatct gggttggatg gggcttcttc tctttaggtt tgtgtttgcc
     2401 ctcccgcctg cttctggttt gtaactagca cagtcccgcc actcagcaca ctcagtatta
     2461 ccgtggagct gtggggttct tgctagaggc cctggtggca gcagtggcag cacttcagtg
     2521 tccttcaggc tgccttgggc ccgaccatcg aacatttggc atctaaccct tgcagagagt
     2581 gggactgggc ggctgccatc ttcttccctc tcatccttct cttcttcctg tgtccatgga
     2641 agagggattt tcaaaaccag tttagtttcc aaaaagtgta catttatggg gagggaggga
     2701 aggggcctaa tttgagtgag agagagagag agagagagtg tgtgtgtgtg tgtgtgtgtg
     2761 tgtgtgtgtg tgtgtgtgtg tgtgtatttt ttatcatttt ttaaaatgca gtactctttg
     2821 tatcagtctg tcatgggtct atagctgttt cagatttttt tgagctgtac ttgagcatct
     2881 gaaactgcaa gaaagaaact gattaaatgt gattcttctt actaaacagg cctgactgct
     2941 gtagctgtgt gacttccctc cctctaccct agcactgccc taccccacag gaaactccca
     3001 tctagtcccc ttgccccata ggctgtgttc tagcaggtga gcaggaagct catggcaggt
     3061 cctagggagg gcagccagag agtctcgctc attccacagg cacagcttct tgactgaacg
     3121 tagcacatct cgtccccacc cactgttcct gtctcttcct agtgactctg atgtttggca
     3181 agaataacat tttctggttt tttaagaaat aattcaacat gaatgtggag agcaaacaaa
     3241 aagatgtagg ttgcaaatgt taccagcatg tgcagtggtg tgtgtgtgcc tatgtgtgtc
     3301 tctgtgtgtg catatatatg tggtgagcat atatatatat tgtgcagcta gggtgaagcc
     3361 agcaaagagg tgtgtatgtt gttatgagac gtttgaaaag aagctgactg cttgataatc
     3421 attctcaggt gtaaagctcc aagtgtaaat aaaagtggca tttaacaaat tttttcagaa
     3481 caaagtccag ctgatgatct ccagcaagca gtaagctaaa ggaacagctg agagccgccg
     3541 atctgcacac agtgcagagg ggtcagctct gtgcttcact gacttggata aggaagggat
     3601 gatgggaaat catggaatta agtagtattt aaacaaaagt ggacgtaata gctcttctgt
     3661 ttgagtaaag ccttggtgtt gctttatctt agtttacatt tttacttttt aaaaagcagc
     3721 aacggatgct tttaaagaag tattgtgact gtagcactgg gaaatgtggc ccgtgcccca
     3781 gcttgttgta gctgtggcat agagttgggc aaaagagcgg tgatggactc attgcaacca
     3841 tacatacttg aagagtccat tgaccgaagc tctttataga ctgttttgta aatggggaaa
     3901 tcacagactg ttgaacgccg cctgcactct aacggtccta gagctgctgg gacctctcct
     3961 atcatgtcag acttggactt ttttccttct tgtttaaagg acataaaatc atagaatcca
     4021 tataatctgt ggagtcatcc tgacccacat gtagatctgt atctaatatt atttggattt
     4081 ggacaagtgt caatgacatg gctgtgtaat aaaattttta ttaaaaatac cacacactgt
     4141 agcccctttg ccactcccca tctgcagctg ctgaaaccct catgctgaga tgccaaaaca
     4201 cggggcaggg aaaaaaagcc caccaaagaa gattgagagt taacccagca actgacctgc
     4261 ctgtctgctg attccagtca acattcagca cctacctgca gtctcagctg aggtggggcc
     4321 tgagccaaga tctcagccag tcaatggaac cttagctttg tcccaccaac tggcttcctg
     4381 gtgggtcaca gtttttctcc tgctggtgaa tgccctcagt ggcctctcca tcaccccaac
     4441 ttcacttcag tgttgagggg cgtaggaccc aaagctgttt tctactcttt ttcagttctg
     4501 ggaagtaaga gagtccatat acctagctca tgctgtctgt agatctgttg tctggtttct
     4561 gacaggcaca gagaaagtct gtttttgtta ttttttttgt gaagtcctga cttcaatctg
     4621 taaatatatt tgtaaataaa ctctggcatt ttaataaggg atcaaaaaaa aaaaaaaaaa
//
