LOCUS       NM_001357956            3082 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus R-spondin 2 (Rspo2), transcript variant 2, mRNA.
ACCESSION   NM_001357956 XM_006520905
VERSION     NM_001357956.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Jasmine, Baraiya,D.H., Kavya,T.T., Mandal,A., Chakraborty,S.,
            Sathish,N., Francis,C.M.R. and Binoy Joseph,D.
  TITLE     Epithelial and mesenchymal compartments of the developing bladder
            and urethra display spatially distinct gene expression patterns
  JOURNAL   Dev Biol 520, 155-170 (2025)
   PUBMED   39798644
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Liu,Y., Fan,M., Yang,J., Mihaljevic,L., Chen,K.H., Ye,Y., Sun,S.
            and Qiu,Z.
  TITLE     KAT6A deficiency impairs cognitive functions through suppressing
            RSPO2/Wnt signaling in hippocampal CA3
  JOURNAL   Sci Adv 10 (20), eadm9326 (2024)
   PUBMED   38758792
  REMARK    GeneRIF: KAT6A deficiency impairs cognitive functions through
            suppressing RSPO2/Wnt signaling in hippocampal CA3.
REFERENCE   3  (bases 1 to 3082)
  AUTHORS   Pappas,M.P., Kawakami,H., Corcoran,D., Chen,K.Q., Scott,E.P.,
            Wong,J., Gearhart,M.D., Nishinakamura,R., Nakagawa,Y. and
            Kawakami,Y.
  TITLE     Sall4 regulates posterior trunk mesoderm development by promoting
            mesodermal gene expression and repressing neural genes in the
            mesoderm
  JOURNAL   Development 151 (5) (2024)
   PUBMED   38345319
REFERENCE   4  (bases 1 to 3082)
  AUTHORS   Guo,D., Pan,H., Lu,X., Chen,Z., Zhou,L., Chen,S., Huang,J.,
            Liang,X., Xiao,Z., Zeng,H., Shao,Y., Qi,W., Xie,D. and Lin,C.
  TITLE     Rspo2 exacerbates rheumatoid arthritis by targeting aggressive
            phenotype of fibroblast-like synoviocytes and disrupting
            chondrocyte homeostasis via Wnt/beta-catenin pathway
  JOURNAL   Arthritis Res Ther 25 (1), 217 (2023)
   PUBMED   37946278
  REMARK    GeneRIF: Rspo2 exacerbates rheumatoid arthritis by targeting
            aggressive phenotype of fibroblast-like synoviocytes and disrupting
            chondrocyte homeostasis via Wnt/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3082)
  AUTHORS   Watanabe,K., Horie,M., Hayatsu,M., Mikami,Y. and Sato,N.
  TITLE     Spatiotemporal expression patterns of R-spondins and their
            receptors, Lgrs, in the developing mouse telencephalon
  JOURNAL   Gene Expr Patterns 49, 119333 (2023)
   PUBMED   37651925
REFERENCE   6  (bases 1 to 3082)
  AUTHORS   Nam,J.S., Turcotte,T.J. and Yoon,J.K.
  TITLE     Dynamic expression of R-spondin family genes in mouse development
  JOURNAL   Gene Expr Patterns 7 (3), 306-312 (2007)
   PUBMED   17035101
REFERENCE   7  (bases 1 to 3082)
  AUTHORS   Nam,J.S., Turcotte,T.J., Smith,P.F., Choi,S. and Yoon,J.K.
  TITLE     Mouse cristin/R-spondin family proteins are novel ligands for the
            Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent
            gene expression
  JOURNAL   J Biol Chem 281 (19), 13247-13257 (2006)
   PUBMED   16543246
REFERENCE   8  (bases 1 to 3082)
  AUTHORS   Lowther,W., Wiley,K., Smith,G.H. and Callahan,R.
  TITLE     A new common integration site, Int7, for the mouse mammary tumor
            virus in mouse mammary tumors identifies a gene whose product has
            furin-like and thrombospondin-like sequences
  JOURNAL   J Virol 79 (15), 10093-10096 (2005)
   PUBMED   16014973
REFERENCE   9  (bases 1 to 3082)
  AUTHORS   Kazanskaya,O., Glinka,A., del Barco Barrantes,I., Stannek,P.,
            Niehrs,C. and Wu,W.
  TITLE     R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling
            and is required for Xenopus myogenesis
  JOURNAL   Dev Cell 7 (4), 525-534 (2004)
   PUBMED   15469841
REFERENCE   10 (bases 1 to 3082)
  AUTHORS   Bell,S.M., Schreiner,C.M., Hess,K.A., Anderson,K.P. and Scott,W.J.
  TITLE     Asymmetric limb malformations in a new transgene insertional
            mutant, footless
  JOURNAL   Mech Dev 120 (5), 597-605 (2003)
   PUBMED   12782276
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161450.3 and AC121609.3.
            
            On Nov 10, 2017 this sequence version replaced XM_006520905.1.
            
            Transcript Variant: This variant (2) contains a distinct 5' UTR and
            lacks an in-frame portion of the 5' coding region, compared to
            variant 1. The resulting isoform (2) has a shorter N-terminus,
            compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049891.1, SRR7652917.848914.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               AC161450.3         159212-159658       c
            448-712             AC161450.3         158571-158835       c
            713-901             AC161450.3         81993-82181         c
            902-1045            AC161450.3         66939-67082         c
            1046-1234           AC161450.3         64617-64805         c
            1235-3082           AC121609.3         183832-185679       c
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 16.73 cM"
     gene            1..3082
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /note="R-spondin 2"
                     /db_xref="GeneID:239405"
                     /db_xref="MGI:MGI:1922667"
     exon            1..447
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     exon            448..712
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     CDS             619..1350
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; cristin-2; mCristin-2; roof plate-specific
                     spondin-2; cysteine-rich and single thrombospondin
                     domain-containing protein 2; R-spondin 2 homolog"
                     /codon_start=1
                     /product="R-spondin-2 isoform 2 precursor"
                     /protein_id="NP_001344885.1"
                     /db_xref="CCDS:CCDS27451.1"
                     /db_xref="GeneID:239405"
                     /db_xref="MGI:MGI:1922667"
                     /translation="MRFCLFSFALIILNCMDYSQCQGNRWRRNKRASYVSNPICKGCL
                     SCSKDNGCSRCQQKLFFFLRREGMRQYGECLHSCPSGYYGHRAPDMNRCARCRIENCD
                     SCFSKDFCTKCKVGFYLHRGRCFDECPDGFAPLDETMECVEGCEVGHWSEWGTCSRNN
                     RTCGFKWGLETRTRQIVKKPAKDTIPCPTIAESRRCKMAMRHCPGGKRTPKAKEKRNK
                     KKRRKLIERAQEQHSVFLATDRVNQ"
     exon            713..901
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1045
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1234
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..3082
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3064..3069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /note="hexamer: ATTAAA"
     polyA_site      3082
                     /gene="Rspo2"
                     /gene_synonym="2610028F08Rik; D430027K22; ftls"
                     /note="major polyA site"
ORIGIN      
        1 atctccatcg gaaagtgcgc tggctacatc ccttcggcct ccgggcagtg ttctgtctcc
       61 cttagctcag gcagcgagaa acttcagctg cgaagtggtg gtggagagag ccctgggagc
      121 agcgactgga cccggacacc aagaagagag tggacgcgcc cctcgactag gaatcgctct
      181 cgcaggcgga gacccagcat ctcagcgcct gcggtcgcgc ttgcccggcc gcgcgctttt
      241 gctaggcgcc gccagccccg aggaccctcg gggtccgcgg acccttctgc agccggcgga
      301 atcctaaagc tgccaagagc tcccggcggg tgtccgcaaa ctttttccga gcccacgtgc
      361 tgaccaaaca gcccggctcg cttccagagc ctggcatgga gcgccgcgcc taggcacgcc
      421 gtgcagcccg agagacgcga gcgcacggtt caccgtggag ggagagatgc tcatcgagcc
      481 aaattgatca ttgcagccgc agggcagtga catctgtctc taagtcctcc ctaggagcgc
      541 gacccgcact gtctccttcc aggagcccgt catttcctcg acttttgaga ggtgtctctc
      601 cccagcccga ccgtccagat gcgtttttgc ctcttctcat ttgccctcat cattctgaac
      661 tgtatggatt acagccagtg ccaaggcaac cgatggagac gcaataagcg agctagttat
      721 gtatcaaatc ccatttgcaa gggttgtttg tcttgttcga aggacaatgg ttgcagccga
      781 tgtcaacaga agttgttctt tttccttcga agagaaggaa tgcgtcagta tggagagtgc
      841 ctgcattcct gcccatcagg gtattatgga caccgagccc cagatatgaa cagatgtgca
      901 cgatgcagaa tagaaaactg tgattcttgc tttagcaaag acttttgtac gaagtgcaaa
      961 gtaggctttt atttgcatag aggccgctgc tttgatgaat gtccagatgg ttttgcaccg
     1021 ttagatgaga ctatggaatg tgtagaaggt tgtgaagttg gtcattggag cgaatgggga
     1081 acgtgtagca gaaacaaccg cacgtgtgga tttaaatggg gtctggaaac cagaacacgg
     1141 cagattgtta aaaagccagc aaaagacaca ataccatgtc cgaccattgc ggagtccagg
     1201 agatgcaaga tggccatgag gcactgtcca ggaggaaaga gaacaccaaa ggcaaaagag
     1261 aagagaaaca agaagaagag gcggaagctg attgagagag cccaagagca gcacagcgtc
     1321 ttcctcgcta cagacagagt gaaccaataa aatacaagaa atagctgggg cattttgagg
     1381 ttttctgttt tgtttatgtt gttgttttgc aaaagtgcac aaagctactc tccagtccac
     1441 actggtggac agcattcctg atcctctgac cagtatccat tttcagtaat gctgcagagg
     1501 gaggtgccca agcatggact cagcgttatt tatgctttga ttggaatctg gggcctgtga
     1561 tggcaggagc ttgttgagct gagtcagcgg gagctgatgc atctgtactc ttgtgatgag
     1621 cacagtgtgt cataagaacc tgtccctggc acggtggacc cacaggaggc acaaggctgt
     1681 agatcaccac cagagaatgc acctgtgcct attttgatgg atggcaatgc taagcaagca
     1741 agcactgttc acttgtgact ttcatttctc acactgtgca ctgtcaaaga caaatgtgca
     1801 tggaaaaatg tttagtgtca cctcatggcg ttctcagcat cagtgacctt caaacggtcc
     1861 tacaatgaga ctgtgttcta gctaggggta tgctgtggaa attcctgcta catttcatct
     1921 tagtgctaac atgtacagat tctgctgcgc tacattcaaa gctcattact gtatatttat
     1981 gctttctctg tgtaacaagt tatacctgat aagatgtcac tttgtttcta gtggttctta
     2041 accatggtct ggtacatggc tattctagtt ttggaaatta acaagtgttt tgttgcctct
     2101 tgttttcttt tgttcctatc atttttggcg ggggtggggt gggcttgatt ctaaccgtaa
     2161 gtataggata agctagtttt gtatatagag tcaaatgact gatgtcagag gatcagtgct
     2221 gatagaactt ccccagttca tgtcaccata cacacagaga gaaagcagca tgaggcatct
     2281 tgccatcaga agccaaattt cttttgagtc ccaaaattga tgacttatga aatatagctg
     2341 aaaacaagat ttgggtgtag ttacttgtat ttattataca atttccaatt acattttttt
     2401 tcaaactcaa aataacccat gactttgagt gataggtcac ttggcaatgt tcttgaatta
     2461 ctggggaagc tgttgtcact aagataatga gagagaaaat agaatggctt cgcccaagtg
     2521 agagccacat cttacatttc tctgttgaat cggaatcaac tatattagaa cagaagcctg
     2581 atggaagctt tctagttaac acacacaagg ccatggtttc aaaaacatct ttgtcccctt
     2641 aggtcagttt gtccttagat tatgaattgg caggttctaa ttgcattatt tccctggctg
     2701 atccaggaaa aagttagaac aaaataagtt gcatagtttt gaggaaacat ccaaagcaag
     2761 gcgaagcctt tccttgcctt gcattggcaa aactacctct ttagcattta tgttgattca
     2821 gaaacatctt gctgatatgt gtagatgttt taagcttcat tgtgaaaata ttgatgcaag
     2881 ataagccata tatgaatgtt gtattcaact ttagggcttg aaattaatcc taaagtgttc
     2941 acctctctcc atgtctattt acactctgtt cctatttact aagagggtag gggtctcctt
     3001 aatatcatac ttcattgtta ataagtcaat gcttgttatg tttcttggct gttgtttttg
     3061 tgcattaaaa actcaaaaat tg
//
