LOCUS       NM_029338                951 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus radial spoke head 9 homolog (Chlamydomonas) (Rsph9),
            mRNA.
ACCESSION   NM_029338
VERSION     NM_029338.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 951)
  AUTHORS   Ho,K.H., Candat,A., Scarpetta,V., Faucourt,M., Weill,S., Salio,C.,
            D'Este,E., Meschkat,M., Wurm,C.A., Kneussel,M., Janke,C.,
            Magiera,M.M., Genovesio,A., Meunier,A., Sassoe-Pognetto,M.,
            Brill,M.S., Spassky,N. and Patrizi,A.
  TITLE     Choroid plexuses carry nodal-like cilia that undergo axoneme
            regression from early adult stage
  JOURNAL   Dev Cell 58 (23), 2641-2651 (2023)
   PUBMED   37890489
REFERENCE   3  (bases 1 to 951)
  AUTHORS   Zhang,X., Xiao,Z., Zhang,J., Xu,C., Liu,S., Cheng,L., Zhou,S.,
            Zhao,S., Zhang,Y., Wu,J., Wang,Y. and Liu,M.
  TITLE     Differential requirements of IQUB for the assembly of radial spoke
            1 and the motility of mouse cilia and flagella
  JOURNAL   Cell Rep 41 (8), 111683 (2022)
   PUBMED   36417862
REFERENCE   4  (bases 1 to 951)
  AUTHORS   Liu,S., Zhang,J., Kherraf,Z.E., Sun,S., Zhang,X., Cazin,C.,
            Coutton,C., Zouari,R., Zhao,S., Hu,F., Fourati Ben Mustapha,S.,
            Arnoult,C., Ray,P.F. and Liu,M.
  TITLE     CFAP61 is required for sperm flagellum formation and male fertility
            in human and mouse
  JOURNAL   Development 148 (23) (2021)
   PUBMED   34792097
REFERENCE   5  (bases 1 to 951)
  AUTHORS   Zheng,W., Li,F., Ding,Z., Liu,H., Zhu,L., Xu,C., Li,J., Gao,Q.,
            Wang,Y., Fu,Z., Peng,C., Yan,X., Zhu,X. and Cong,Y.
  TITLE     Distinct architecture and composition of mouse axonemal radial
            spoke head revealed by cryo-EM
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   34871179
REFERENCE   6  (bases 1 to 951)
  AUTHORS   Zou,W., Lv,Y., Liu,Z.I., Xia,P., Li,H. and Jiao,J.
  TITLE     Loss of Rsph9 causes neonatal hydrocephalus with abnormal
            development of motile cilia in mice
  JOURNAL   Sci Rep 10 (1), 12435 (2020)
   PUBMED   32709945
  REMARK    GeneRIF: Loss of Rsph9 causes neonatal hydrocephalus with abnormal
            development of motile cilia in mice.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 951)
  AUTHORS   Zhu,L., Liu,H., Chen,Y., Yan,X. and Zhu,X.
  TITLE     Rsph9 is critical for ciliary radial spoke assembly and central
            pair microtubule stability
  JOURNAL   Biol Cell 111 (2), 29-38 (2019)
   PUBMED   30383886
  REMARK    GeneRIF: Rsph9 was specifically expressed in mouse tissues
            containing motile cilia and upregulated during multiciliation.
REFERENCE   8  (bases 1 to 951)
  AUTHORS   Abbasi,F., Miyata,H., Shimada,K., Morohoshi,A., Nozawa,K.,
            Matsumura,T., Xu,Z., Pratiwi,P. and Ikawa,M.
  TITLE     RSPH6A is required for sperm flagellum formation and male fertility
            in mice
  JOURNAL   J Cell Sci 131 (19) (2018)
   PUBMED   30185526
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 951)
  AUTHORS   Castleman,V.H., Romio,L., Chodhari,R., Hirst,R.A., de Castro,S.C.,
            Parker,K.A., Ybot-Gonzalez,P., Emes,R.D., Wilson,S.W., Wallis,C.,
            Johnson,C.A., Herrera,R.J., Rutman,A., Dixon,M., Shoemark,A.,
            Bush,A., Hogg,C., Gardiner,R.M., Reish,O., Greene,N.D.,
            O'Callaghan,C., Purton,S., Chung,E.M. and Mitchison,H.M.
  TITLE     Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause
            primary ciliary dyskinesia with central-microtubular-pair
            abnormalities
  JOURNAL   Am J Hum Genet 84 (2), 197-209 (2009)
   PUBMED   19200523
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110562.17.
            
            On Jul 17, 2018 this sequence version replaced NM_029338.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019423.1, AK006440.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-279               AC110562.17        154446-154724       c
            280-445             AC110562.17        152071-152236       c
            446-575             AC110562.17        147266-147395       c
            576-722             AC110562.17        145497-145643       c
            723-951             AC110562.17        139794-140022       c
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.87 cM"
     gene            1..951
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /note="radial spoke head 9 homolog (Chlamydomonas)"
                     /db_xref="GeneID:75564"
                     /db_xref="MGI:MGI:1922814"
     exon            1..279
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..883
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /codon_start=1
                     /product="radial spoke head protein 9 homolog"
                     /protein_id="NP_083614.1"
                     /db_xref="CCDS:CCDS28820.1"
                     /db_xref="GeneID:75564"
                     /db_xref="MGI:MGI:1922814"
                     /translation="MDADSLLLSLELASGSGQGLSPDRRASLLTSLMLVKRDYRFARV
                     LFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMAMQISVVSGR
                     FMGDPSHEYEHTELQKVNEGEKVFDEEVVVQIKEETRLVSIIDQIDKAVAIIPRGALF
                     KTPFGVTHVNRTFEGLPLSEVRKLSSYFHFREAIDLKNKTLLEKSDLEPSLDFLDSLE
                     YDIPRGSWSIQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYIYVGTGEKNMDLPFML
                     "
     exon            280..445
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            446..575
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            576..722
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            723..951
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      925..930
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /note="hexamer: CATAAA"
     polyA_site      950
                     /gene="Rsph9"
                     /gene_synonym="1700027N10Rik"
                     /note="major polyA site"
ORIGIN      
        1 atggagacag cttctacccg ccagactagc ttggaggaga acggctggcc tgatggacgc
       61 cgacagcctc ttgttgtctc tggagttggc gtctggcagt gggcagggac tcagccctga
      121 ccgtcgggcc tcactgctca cgtcccttat gctggtgaag cgcgattacc gcttcgcgag
      181 ggtccttttc tggggccgca tccttggcct tgtggcggat tactacatcg cacagggctt
      241 gagtgaggac cagctcgcac cacgcaagac gctctacagc ctgaactgca cagagtggag
      301 tctcttgccc cctgccacag aggagatggc catgcagata tctgtggtga gtggccgttt
      361 catgggcgac ccttcacacg agtatgaaca cacagagttg cagaaggtta acgaaggaga
      421 gaaggtcttt gatgaagaag tggtggtcca gatcaaggaa gagactcgct tggtgtccat
      481 cattgaccag attgacaagg ctgtagctat catccctcga ggtgccctct tcaagacccc
      541 ttttggagtc acccatgtca atcggacctt tgaaggcctg cccctgtccg aggtcaggaa
      601 gcttagctcg tacttccact tcagggaggc tattgatctg aagaacaaga ccttgctgga
      661 gaagtcggac ttggagccct cgctggattt cctggactcc ctggaatatg acatccccag
      721 agggtcttgg agcatccaga tggaaagggg caacgcactg gtggtgctgc gcagcctgct
      781 ctggccaggc ctcaccttct accacgctcc tcgcaccaag aactatggct acatctacgt
      841 aggcacagga gagaagaaca tggacttgcc cttcatgctg taggaaggca ggctggatat
      901 ttatgtgggg tcgaaacact ctttcataaa gttcaactgt tgggcctttt t
//
