LOCUS       NM_031255               2355 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus radial spoke head 6 homolog A (Chlamydomonas)
            (Rsph6a), transcript variant 1, mRNA.
ACCESSION   NM_031255 XM_986158
VERSION     NM_031255.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Zhang,X., Xiao,Z., Zhang,J., Xu,C., Liu,S., Cheng,L., Zhou,S.,
            Zhao,S., Zhang,Y., Wu,J., Wang,Y. and Liu,M.
  TITLE     Differential requirements of IQUB for the assembly of radial spoke
            1 and the motility of mouse cilia and flagella
  JOURNAL   Cell Rep 41 (8), 111683 (2022)
   PUBMED   36417862
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zheng,W., Li,F., Ding,Z., Liu,H., Zhu,L., Xu,C., Li,J., Gao,Q.,
            Wang,Y., Fu,Z., Peng,C., Yan,X., Zhu,X. and Cong,Y.
  TITLE     Distinct architecture and composition of mouse axonemal radial
            spoke head revealed by cryo-EM
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   34871179
REFERENCE   3  (bases 1 to 2355)
  AUTHORS   Miyata,H., Abbasi,F., Visconti,P.E. and Ikawa,M.
  TITLE     CRISPR/CAS9-mediated amino acid substitution reveals
            phosphorylation residues of RSPH6A are not essential for male
            fertility in micedagger
  JOURNAL   Biol Reprod 103 (5), 912-914 (2020)
   PUBMED   32901808
REFERENCE   4  (bases 1 to 2355)
  AUTHORS   Paudel,B., Gervasi,M.G., Porambo,J., Caraballo,D.A., Tourzani,D.A.,
            Mager,J., Platt,M.D., Salicioni,A.M. and Visconti,P.E.
  TITLE     Sperm capacitation is associated with phosphorylation of the
            testis-specific radial spoke protein Rsph6adagger
  JOURNAL   Biol Reprod 100 (2), 440-454 (2019)
   PUBMED   30239614
  REMARK    GeneRIF: The findings that Rsph6a is more phosphorylated during
            capacitation and is predicted to function in axonemal localization
            make Rsph6a a candidate protein mediating signaling processes in
            the sperm flagellum.
REFERENCE   5  (bases 1 to 2355)
  AUTHORS   Abbasi,F., Miyata,H., Shimada,K., Morohoshi,A., Nozawa,K.,
            Matsumura,T., Xu,Z., Pratiwi,P. and Ikawa,M.
  TITLE     RSPH6A is required for sperm flagellum formation and male fertility
            in mice
  JOURNAL   J Cell Sci 131 (19) (2018)
   PUBMED   30185526
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2355)
  AUTHORS   Eriksson,M., Ansved,T., Anvret,M. and Carey,N.
  TITLE     A mammalian radial spokehead-like gene, RSHL1, at the myotonic
            dystrophy-1 locus
  JOURNAL   Biochem Biophys Res Commun 281 (4), 835-841 (2001)
   PUBMED   11237735
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC170864.2.
            
            On Dec 30, 2022 this sequence version replaced NM_031255.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF329192.1, BC137992.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-673               AC170864.2         31589-32261         c
            674-905             AC170864.2         29194-29425         c
            906-1670            AC170864.2         20891-21655         c
            1671-1815           AC170864.2         19729-19873         c
            1816-1933           AC170864.2         18811-18928         c
            1934-2355           AC170864.2         12503-12924         c
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.46 cM"
     gene            1..2355
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /note="radial spoke head 6 homolog A (Chlamydomonas)"
                     /db_xref="GeneID:83434"
                     /db_xref="MGI:MGI:1927643"
     exon            1..673
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            674..905
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1670
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1815
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1933
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2355
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2288..2293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /note="hexamer: AATAAA"
     polyA_site      2307
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
                     /note="major polyA site"
     polyA_site      2355
                     /gene="Rsph6a"
                     /gene_synonym="Rshl1; RSP4"
ORIGIN      
        1 aggcaggcag ggtccaggat agggcagcac actttttccg aggcatgggg gaaccaccgc
       61 ccaatcctga cccctctcaa acccggaggg cttcccaagg ctcagaaagg gcacggagtc
      121 aagagtactc tcagcctctg ttaaccatcc cagaggatgg gttgaaccga ccacctccgc
      181 agcggggcag tcggagcagt cagggcagtc aggacctgca gggaactggt ctaccccact
      241 ggccacagag gtccagcctg gtcccagatg ttcaaggtga tgaaggcacc gagtaccacc
      301 agtccatgcc cttaggctac acgccaggct ttcccatgga gttctcgcaa cagggttacc
      361 tggatgatag caggatgatg gagcagttcc cacagggcca ggacctctta gaacaactgg
      421 agtctaccta ccaaggctcg gcttctggaa tcctgggcca gctcaacttg tatccaagag
      481 aggatgagat attcagccag gacacacagc acggacccta cctgagagac gacccctccc
      541 tccacctgag gccctctgac ctgggcttca tgcccattgt tggagaggta cctgaccccg
      601 agcctcgtga gctggcaata cagaacgcca aggcctacct gctgcggacc agcatgagct
      661 gcaacctcag cctgtatgag cacctggtga acctgctgac caagatcctg aaccagcgcc
      721 ctgaggaccc cttgtccatc ctggagtctc tgaaccgcac catgcagtgg gaatggtttc
      781 accccaagct ggacacgctg cgggacgacc ccgagatgca gcccacctac gagatggccg
      841 aaaagcaaaa ggctctgttt atcaggggtg gaggagaagg cgaacaggaa atggaagagg
      901 aggtgactga cagcccggtg cccaacatca tggagaccgc cttctacttc gagcaggcgg
      961 gcgtggggct gagctcggac gaaagcttcc gaatcttcct ggccctgaag cagctggtgg
     1021 agcagcagcc catccacatg tgccgcttct ggggcaagat cctgggcctg agccgcagct
     1081 acctggtggc tgaggtggag ttccgcgagg gcgaggagga gggagaggag gaggaggtgg
     1141 aggagatgat ggagggaggc gaggtcttgg agacgcacgg tgaggaggag ggagaggagg
     1201 atgaggagaa ggtggtggac agcgtgccca agccacagtg gaagccgccg cccatcatcc
     1261 ccaaggagga gagccgcagt ggcaccaaca aatacctgta cttcgtgtgc aacgagccag
     1321 gccgcccgtg gacgcgcctg ccgcacgtga cgcccgcgca gatcgtgtgc gcgcgcaaga
     1381 tcaaaaagtt cttcactggc ttcctggaca cgccggtcat cagctatccg cccttcccgg
     1441 gcaacgaggc caactatctc cgcgcgcaga ttgcgcgcat ctccgccgcc acacacatca
     1501 gcccgctggg cttctaccag ttcggcgagg aggagggcga tgaggaggag gagggcggcg
     1561 cgggacgcga ttcctttgag gagaaccccg actttgaggg catccccgtg ttggagctcg
     1621 tggactccat ggccaactgg gtacatcaca ctcagcacat cctgcctcag gggcgctgta
     1681 catgggtgaa cccactgcag aaaacagagg aggaagaaga gctaggggag gaggaagaga
     1741 aggcagacga ggcaatggag gaggtggagc aggaggtcgg tcctccgctt ctgactccgc
     1801 tctcagaaga tgcagaaatc atgcacctgt cgccctggac cacccgcctc tcctgcagcc
     1861 tcagccctca gtactcggtg gccatcgtgc gctccaacct ctggccaggg gcctatgcct
     1921 acgccactgg caagaagttt gagaacatat acattggctg gggtcacaag tatagccccg
     1981 agaacttcaa cccaatgctg ccagctctga ttcagcaaga gtaccccagc ggccccgaaa
     2041 tcacggagat gagtgacccc acagtggagg aggagcaggc cctgaaggct gctcaggagc
     2101 aggccctggc agccgctgag gaagaggagg aagatgagga agaggaggag gatgaagacc
     2161 tggaggactg aggccccaag ctccttccca tagcccttcc ccttattcgt agcaacagat
     2221 tgtattttcc tattggtttc gttccctgtc cttgaaggac acaggtagga aaccacccag
     2281 tgctccaaat aaaactgccc cagcacagct gcttagtcac atattcattc agtatcctgg
     2341 gcagctggct catca
//
