LOCUS       NM_001162957            3650 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus radial spoke head 4 homolog A (Chlamydomonas)
            (Rsph4a), mRNA.
ACCESSION   NM_001162957 XM_137041 XM_906805
VERSION     NM_001162957.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Zheng,W., Li,F., Ding,Z., Liu,H., Zhu,L., Xu,C., Li,J., Gao,Q.,
            Wang,Y., Fu,Z., Peng,C., Yan,X., Zhu,X. and Cong,Y.
  TITLE     Distinct architecture and composition of mouse axonemal radial
            spoke head revealed by cryo-EM
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   34871179
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Ide,T., Twan,W.K., Lu,H., Ikawa,Y., Lim,L.X., Henninger,N.,
            Nishimura,H., Takaoka,K., Narasimhan,V., Yan,X., Shiratori,H.,
            Roy,S. and Hamada,H.
  TITLE     CFAP53 regulates mammalian cilia-type motility patterns through
            differential localization and recruitment of axonemal dynein
            components
  JOURNAL   PLoS Genet 16 (12), e1009232 (2020)
   PUBMED   33347437
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3650)
  AUTHORS   Yoke,H., Ueno,H., Narita,A., Sakai,T., Horiuchi,K., Shingyoji,C.,
            Hamada,H. and Shinohara,K.
  TITLE     Rsph4a is essential for the triplet radial spoke head assembly of
            the mouse motile cilia
  JOURNAL   PLoS Genet 16 (3), e1008664 (2020)
   PUBMED   32203505
  REMARK    GeneRIF: Results suggest Rsph4a is a generic component of radial
            spoke heads, and could explain the severe phenotype of human PCD
            patients with RSPH4A mutation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3650)
  AUTHORS   Abbasi,F., Miyata,H., Shimada,K., Morohoshi,A., Nozawa,K.,
            Matsumura,T., Xu,Z., Pratiwi,P. and Ikawa,M.
  TITLE     RSPH6A is required for sperm flagellum formation and male fertility
            in mice
  JOURNAL   J Cell Sci 131 (19) (2018)
   PUBMED   30185526
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3650)
  AUTHORS   Stauber,M., Weidemann,M., Dittrich-Breiholz,O., Lobschat,K.,
            Alten,L., Mai,M., Beckers,A., Kracht,M. and Gossler,A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev Biol 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   6  (bases 1 to 3650)
  AUTHORS   Shinohara,K., Chen,D., Nishida,T., Misaki,K., Yonemura,S. and
            Hamada,H.
  TITLE     Absence of Radial Spokes in Mouse Node Cilia Is Required for
            Rotational Movement but Confers Ultrastructural Instability as a
            Trade-Off
  JOURNAL   Dev Cell 35 (2), 236-246 (2015)
   PUBMED   26506310
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153961.2.
            
            On or before Jul 2, 2009 this sequence version replaced
            XM_906805.2, XM_137041.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC138000.1, AK038980.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-728               AC153961.2         21170-21897         c
            729-963             AC153961.2         18707-18941         c
            964-1701            AC153961.2         17258-17995         c
            1702-1846           AC153961.2         15358-15502         c
            1847-1964           AC153961.2         13929-14046         c
            1965-3650           AC153961.2         10987-12672         c
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 18.84 cM"
     gene            1..3650
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /note="radial spoke head 4 homolog A (Chlamydomonas)"
                     /db_xref="GeneID:212892"
                     /db_xref="MGI:MGI:3027894"
     exon            1..728
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..2196
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /note="radial spokehead-like 3; radial spoke head-like
                     protein 3"
                     /codon_start=1
                     /product="radial spoke head protein 4 homolog A"
                     /protein_id="NP_001156429.1"
                     /db_xref="CCDS:CCDS48534.1"
                     /db_xref="GeneID:212892"
                     /db_xref="MGI:MGI:3027894"
                     /translation="MENSTSLKQEKENQEPGEAERLWQGESDVSPQEPGPPSPEYREE
                     EQRTDTEPAPRMSPSWSHQSRVSLSTGDLTAGPEVSSSPPPPPLQFHSTPLNTETTQD
                     PVAASPTEKTANGIADTGTPYSDPWESSSAAKQSTSHYTSHAEESTFPQSQTPQPDLC
                     GLRDASRNKSKHKGLRFDLLQEEGSDSNCDPDQPEVGASEAAQSMLEVAIQNAKAYLL
                     STSSKSGLNLYDHLSKVLTKILDERPADAVDIIENISQDVKMAHFNKKLDTLHNEYEM
                     LPAYEIAETQKALFLQGHLEGADSELEEEMAESSLPNVMESAYYFEQAGVGLGTDETY
                     RVFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFRDGEDEEEVEEEGIAEERDN
                     GGSEAGEEEEEELPKSLYKAPQVIPKEESRTGANKYVYFVCNVPGRPWVRLPSVTPAQ
                     IVTARKIKKFFTGRLDAAVISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEE
                     GEEEEVEGGRDSYEENPDFEGIQVIDLVESLSNWVHHVQYILPQGRCNWFNPIQKDED
                     EEEEEEEDEEKGEEPDYIEQEVGPPLLTPISEDLGIQNIPSWTTQLSSNLIPQYAIAV
                     LRSNLWPGAYAFSNGKKFENFYIGWGHKYCVENYTPPSPPPVYQEYPSGPEITEMNDP
                     SVEEEQAFRMTQEPVALSTEENEGTEDEDEDDED"
     exon            729..963
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1701
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1846
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1964
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..3650
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3491..3496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /note="hexamer: AATAAA"
     polyA_site      3509
                     /gene="Rsph4a"
                     /gene_synonym="A230081C05; Rshl3"
                     /note="major polyA site"
ORIGIN      
        1 tccatatcgt cacgctccgt tcacgtgact tatttcttaa ctcctatgga aaactctacc
       61 tctctgaaac aagaaaagga aaatcaagag ccaggggaag cagagagatt atggcaagga
      121 gagtcagacg tttctcccca ggagcctggt cccccatcac ctgagtaccg ggaagaggag
      181 caaaggacag acaccgaacc cgcacccaga atgagcccct cttggagcca ccagtctaga
      241 gtcagcctgt ctacgggaga cctcaccgct ggaccagaag tgtcatcctc ccctccccct
      301 cctcctctgc agtttcattc cactcctctc aacactgaaa ctactcagga tcctgtagct
      361 gcatcaccca cagagaagac cgctaatgga atcgctgaca ccgggacacc ttattctgat
      421 ccttgggaat catcttctgc tgcaaagcaa tcaacttctc actacacaag ccacgccgag
      481 gaaagtactt tcccgcagtc tcagacaccc caacctgacc tctgtgggct aagggatgcc
      541 agcaggaaca agtccaagca caaagggctg agatttgacc ttttacaaga ggaaggctca
      601 gacagtaact gtgacccaga ccagcctgag gttggagcca gtgaagcagc ccagagtatg
      661 cttgaggtgg ccattcagaa tgcaaaagcc tacctgctga gtaccagcag caagtctggc
      721 ttaaatctat atgaccatct ttctaaagtg ttgaccaaga tactagatga gcgtcctgca
      781 gatgctgttg acatcattga aaatataagc caagatgtga aaatggcaca ttttaataaa
      841 aaattagata cactccacaa tgaatatgag atgcttccag catatgaaat agcagagaca
      901 caaaaggcac tttttctcca gggacatttg gaaggtgctg attcagaact ggaggaggaa
      961 atggcagaaa gttctctgcc caatgtgatg gaatcagctt attattttga acaagctgga
     1021 gttggtttgg ggacagatga gacttaccga gtattcctcg ccctcaagca acttactgat
     1081 acacacccaa tccaaagatg tcgcttctgg ggcaagatct tgggtctgga aatgaactat
     1141 attgttgctg aagtggaatt tcgtgatggt gaagatgaag aggaggtgga agaagaaggg
     1201 atagctgagg agagggacaa tggaggaagt gaggccggtg aagaggagga ggaggaatta
     1261 ccgaagtcct tgtataaggc cccacaggtt atacctaagg aagaaagtag aacgggtgcc
     1321 aacaagtatg tctattttgt ttgcaatgtg cctggaaggc catgggtaag gttaccatca
     1381 gttacacctg cacaaattgt cactgcaaga aaaatcaaga aatttttcac tgggcgacta
     1441 gatgcagctg tcataagcta cccacccttc ccaggaaatg agagcaatta cttaagagca
     1501 caaattgccc gcatttcagc agggacccat gtcagtcctc taggattcta tcagtttggt
     1561 gaagaggaag gagaggaaga agaggtggaa ggtggtcgag acagctatga ggaaaaccct
     1621 gactttgaag gcatccaagt gattgacttg gtggagtctc tatccaattg ggttcatcac
     1681 gtgcagtata ttcttcctca gggtcgctgt aattggttca accctataca aaaagatgaa
     1741 gatgaagaag aggaagagga agaagatgaa gaaaaggggg aagaacctga ctacatagag
     1801 caggaagtcg gacctcctct tttgacacca atatctgaag atttggggat tcagaatata
     1861 ccctcttgga caacacagct gtcttcaaat ctcattccac agtatgccat tgctgtcctt
     1921 cgctccaatc tttggcctgg agcatatgct ttttccaatg gcaagaagtt tgaaaacttc
     1981 tacataggct ggggtcataa gtactgtgtg gagaattaca cacccccaag cccaccacca
     2041 gtttatcaag aatatcccag tggaccagaa attacagaaa tgaatgatcc cagtgtggag
     2101 gaggaacagg ctttcaggat gacacaagag ccagttgcac tttcaactga ggagaacgaa
     2161 ggaactgaag atgaagatga ggacgatgag gactaataca aaactagccc aaccttttat
     2221 ggcactaaca cacacaaatc ttttatgggg gactgatttt atagatgcat atattgaata
     2281 cacacacaca catatataca cacatgtgtg tctatgtaac acacacacat acatactttg
     2341 aagatgatgg tacaaaatat tcctttaaag ttaccaagtg tgtggggggg cagggatgat
     2401 aaacagatgt ctcagtgttt ataaaaaaat tttgctctga ggacaggaat ccagttgcta
     2461 ggacctatgt cagggggctt accactacct gcaactccaa gtctaggaaa tctaatgctc
     2521 tcttctatcc tccacaggca cagcacatgt gcatgtttgc taacacaagt aaatcttcca
     2581 aaaagatttt aaaagctaga tgtggtgaaa taaaccttta accccaatac ttgggaggca
     2641 gagggaggaa gatttctatg agtccaaagc cagcctggtc tggatagcaa gctccagtga
     2701 agcaaacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
     2761 acacgaatga tattggaaac tgtatataga actgccagta tttacagaaa gtagccagtg
     2821 gattttccag aagctcacca catcaccgtt ttaatgaata tagcttatgt aattaacact
     2881 tatataatca tgctttgaaa agatctcaat ttctgttttc tttttctcct tgacagggtc
     2941 aaactatata attctggctg tcctggaact cgctatgcag accaggttgg ctataaaatc
     3001 acatagagag gcctctgcct tttaagtgct aggattgaag gtgtgaatgt tcaaagttaa
     3061 tttccaacat ggcaagtatt gattgctata actcacaatg cttctaggaa aattccagtt
     3121 tttgagtgtc aagggatata gaatgcaaaa ctttgataac ctggactaga aagactcagt
     3181 ggttaccagt acttgttact cttgctgagg acctgggttc gattcccagc atccacatga
     3241 tagcttgaaa tcatcagttc taagggatct agtatccctt tctgacatcc atgtgtaaca
     3301 cacacacaca tgcaaaatac tcataaacaa acctctaata acctaaagca tttattttag
     3361 cctgtcagaa ctggatggag atgtattaaa tctgtagttg cttgtgctga agccaatgaa
     3421 actgtgtata aatactgtgc atgtgcacgc attctctttc ctgggtgagt gttcacaatt
     3481 ccataagtgg aataaaacct gtaagggcat tcaaatgttt agagtttttg tttttcccca
     3541 acttctccat caaatttctt cttcatccta aattagtcag tggaaatgaa ttacttagag
     3601 atgggaggta gtgttatcca gacactttag acttacgttc tctatcactc
//
