LOCUS       NM_025290               1104 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus radial spoke head 1 homolog (Chlamydomonas) (Rsph1),
            transcript variant 1, mRNA.
ACCESSION   NM_025290 XM_992342
VERSION     NM_025290.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Wang,M., Kang,J., Shen,Z., Hu,Y., Chen,M., Cui,X., Liu,H. and
            Gao,F.
  TITLE     CCDC189 affects sperm flagellum formation by interacting with
            CABCOCO1
  JOURNAL   Natl Sci Rev 10 (9), nwad181 (2023)
   PUBMED   37601242
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Zhang,X., Xiao,Z., Zhang,J., Xu,C., Liu,S., Cheng,L., Zhou,S.,
            Zhao,S., Zhang,Y., Wu,J., Wang,Y. and Liu,M.
  TITLE     Differential requirements of IQUB for the assembly of radial spoke
            1 and the motility of mouse cilia and flagella
  JOURNAL   Cell Rep 41 (8), 111683 (2022)
   PUBMED   36417862
REFERENCE   3  (bases 1 to 1104)
  AUTHORS   Zheng,W., Li,F., Ding,Z., Liu,H., Zhu,L., Xu,C., Li,J., Gao,Q.,
            Wang,Y., Fu,Z., Peng,C., Yan,X., Zhu,X. and Cong,Y.
  TITLE     Distinct architecture and composition of mouse axonemal radial
            spoke head revealed by cryo-EM
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   34871179
REFERENCE   4  (bases 1 to 1104)
  AUTHORS   Yoke,H., Ueno,H., Narita,A., Sakai,T., Horiuchi,K., Shingyoji,C.,
            Hamada,H. and Shinohara,K.
  TITLE     Rsph4a is essential for the triplet radial spoke head assembly of
            the mouse motile cilia
  JOURNAL   PLoS Genet 16 (3), e1008664 (2020)
   PUBMED   32203505
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1104)
  AUTHORS   Rachev,E., Schuster-Gossler,K., Fuhl,F., Ott,T., Tveriakhina,L.,
            Beckers,A., Hegermann,J., Boldt,K., Mai,M., Kremmer,E., Ueffing,M.,
            Blum,M. and Gossler,A.
  TITLE     CFAP43 modulates ciliary beating in mouse and Xenopus
  JOURNAL   Dev Biol 459 (2), 109-125 (2020)
   PUBMED   31884020
REFERENCE   6  (bases 1 to 1104)
  AUTHORS   Hui,L., Lu,J., Han,Y. and Pilder,S.H.
  TITLE     The mouse T complex gene Tsga2, encoding polypeptides located in
            the sperm tail and anterior acrosome, maps to a locus associated
            with sperm motility and sperm-egg interaction abnormalities
  JOURNAL   Biol Reprod 74 (4), 633-643 (2006)
   PUBMED   16354795
  REMARK    GeneRIF: Tsga2 fulfills many of the prerequisites required to make
            it a strong candidate for Ccub1, an element of Cucb positioned
            within a genomic interval spanning approximately 0.6 Mb immediately
            distal to Dnahc8.
REFERENCE   7  (bases 1 to 1104)
  AUTHORS   Ju,T.K. and Huang,F.L.
  TITLE     MSAP, the meichroacidin homolog of carp (Cyprinus carpio), differs
            from the rodent counterpart in germline expression and involves
            flagellar differentiation
  JOURNAL   Biol Reprod 71 (5), 1419-1429 (2004)
   PUBMED   15215198
REFERENCE   8  (bases 1 to 1104)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   9  (bases 1 to 1104)
  AUTHORS   Davisson,M.T., Bechtel,L.J., Akeson,E.C., Fortna,A., Slavov,D. and
            Gardiner,K.
  TITLE     Evolutionary breakpoints on human chromosome 21
  JOURNAL   Genomics 78 (1-2), 99-106 (2001)
   PUBMED   11707078
REFERENCE   10 (bases 1 to 1104)
  AUTHORS   Tsuchida,J., Nishina,Y., Wakabayashi,N., Nozaki,M., Sakai,Y. and
            Nishimune,Y.
  TITLE     Molecular cloning and characterization of meichroacidin (male
            meiotic metaphase chromosome-associated acidic protein)
  JOURNAL   Dev Biol 197 (1), 67-76 (1998)
   PUBMED   9578619
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154652.2.
            
            On Jul 14, 2018 this sequence version replaced NM_025290.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049584.1, AK005739.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                AC154652.2         45392-45486         c
            96-209              AC154652.2         41715-41828         c
            210-315             AC154652.2         41507-41612         c
            316-406             AC154652.2         34533-34623         c
            407-542             AC154652.2         34004-34139         c
            543-614             AC154652.2         29945-30016         c
            615-774             AC154652.2         26978-27137         c
            775-903             AC154652.2         26288-26416         c
            904-1104            AC154652.2         23209-23409         c
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 15.94 cM"
     gene            1..1104
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /note="radial spoke head 1 homolog (Chlamydomonas)"
                     /db_xref="GeneID:22092"
                     /db_xref="MGI:MGI:1194909"
     exon            1..95
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..947
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     meichroacidin; testis-specific gene A2 protein; male
                     meiotic metaphase chromosome-associated acidic protein"
                     /codon_start=1
                     /product="radial spoke head 1 homolog isoform 1"
                     /protein_id="NP_079566.1"
                     /db_xref="CCDS:CCDS28604.1"
                     /db_xref="GeneID:22092"
                     /db_xref="MGI:MGI:1194909"
                     /translation="MSDLGSEELEEEGENDLGEYEGERNEVGERHGHGKARLPNGDTY
                     EGSYEFGKRHGQGTYKFKNGARYTGDYVKNKKHGQGTFIYPDGSRYEGEWADDQRHGQ
                     GVYYYVNNDTYTGEWFNHQRHGQGTYLYAETGSKYVGTWVHGQQEGAAELIHLNHRYQ
                     GKFMNKNPVGPGKYVFDIGCEQHGEYRLTDTERGEEEEEEETLVNIVPKWKALNITEL
                     ALWTPTLSEEQPPPEGQGQEEPQGLTGVGDPSEDIQAEGFEGELEPRGADEDVDTFRQ
                     ESQENSYDIDQGNLNFDEEPSDLQD"
     exon            96..209
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            210..315
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            316..406
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            407..542
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            543..614
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..774
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            775..903
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1104
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1074..1079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /note="hexamer: AATAAA"
     polyA_site      1104
                     /gene="Rsph1"
                     /gene_synonym="MCA; Tsga2"
                     /note="major polyA site"
ORIGIN      
        1 gatagagacg ttgctcctga gatcctgtct gaaaccagat catgtcggac ctgggctctg
       61 aggagttaga ggaggaggga gagaacgacc ttggggagta cgaaggggag cgcaatgagg
      121 tgggagaacg acacggacat gggaaagcac gactgcccaa cggggacaca tatgaaggaa
      181 gctatgagtt tggaaaaaga catggccagg ggacttacaa atttaagaat ggggcccggt
      241 acaccggaga ctatgtcaaa aataaaaagc acggccaagg cacctttatc tatccagatg
      301 gatccagata tgaaggggag tgggctgatg accagaggca cggccaaggc gtgtactact
      361 atgtcaacaa tgacacctac acaggggagt ggttcaatca tcaaaggcac gggcaaggca
      421 cctacctcta cgcagagacc ggcagtaagt atgtcggtac ctgggtgcac ggacagcagg
      481 agggtgctgc cgagctcatc cacctaaacc acaggtacca gggcaagttc atgaacaaaa
      541 atcctgtggg ccctggaaag tacgtatttg atattggatg cgaacagcac ggtgaatatc
      601 gcctcacaga tacggaaaga ggagaagagg aggaggagga agagacatta gtgaacatcg
      661 ttccaaaatg gaaagctctc aacatcacag aattggccct gtggactcca accctctccg
      721 aggagcagcc tccccctgag ggccaaggcc aggaggaacc gcagggactt actggcgtgg
      781 gtgacccctc agaagacatc caggcagaag gttttgaggg cgagctggag ccgagggggg
      841 ccgatgaaga cgtagacacg ttcaggcagg agagccagga gaacagctac gacatagacc
      901 agggaaactt gaactttgat gaagaaccgt cagacctcca ggattaagat agtggaggca
      961 gaagaccaca gacacaccag gcgtgcctta gttaacacca gtcagctagg gctggtatcc
     1021 accacctgtc aatctctctc tcttagctgt taagttgttt tttcggttaa caaaataaat
     1081 ctccaggtgt tcagtgttga caaa
//
