LOCUS       NM_001081267           11129 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus remodeling and spacing factor 1 (Rsf1), mRNA.
ACCESSION   NM_001081267 NM_001008500 XM_620529
VERSION     NM_001081267.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Min,S., Kim,K., Kim,S.G., Cho,H. and Lee,Y.
  TITLE     Chromatin-remodeling factor, RSF1, controls p53-mediated
            transcription in apoptosis upon DNA strand breaks
  JOURNAL   Cell Death Dis 9 (11), 1079 (2018)
   PUBMED   30348983
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11129)
  AUTHORS   Zhang,Z., Jones,A.E., Wu,W., Kim,J., Kang,Y., Bi,X., Gu,Y.,
            Popov,I.K., Renfrow,M.B., Vassylyeva,M.N., Vassylyev,D.G.,
            Giles,K.E., Chen,D., Kumar,A., Fan,Y., Tong,Y., Liu,C.F., An,W.,
            Chang,C., Luo,J., Chow,L.T. and Wang,H.
  TITLE     Role of remodeling and spacing factor 1 in histone H2A
            ubiquitination-mediated gene silencing
  JOURNAL   Proc Natl Acad Sci U S A 114 (38), E7949-E7958 (2017)
   PUBMED   28855339
  REMARK    GeneRIF: these data identify RSF1 as a H2Aub reader that
            contributes to H2Aub-mediated gene silencing by maintaining a
            stable nucleosome pattern at promoter regions.
REFERENCE   4  (bases 1 to 11129)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   5  (bases 1 to 11129)
  AUTHORS   Funato,N., Kokubo,H., Nakamura,M., Yanagisawa,H. and Saga,Y.
  TITLE     Specification of jaw identity by the Hand2 transcription factor
  JOURNAL   Sci Rep 6, 28405 (2016)
   PUBMED   27329940
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11129)
  AUTHORS   Lee,H.S., Park,Y.Y., Cho,M.Y., Chae,S., Yoo,Y.S., Kwon,M.H.,
            Lee,C.W. and Cho,H.
  TITLE     The chromatin remodeller RSF1 is essential for PLK1 deposition and
            function at mitotic kinetochores
  JOURNAL   Nat Commun 6, 7904 (2015)
   PUBMED   26259146
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 11129)
  AUTHORS   Thompson,P.J., Norton,K.A., Niri,F.H., Dawe,C.E. and McDermid,H.E.
  TITLE     CECR2 is involved in spermatogenesis and forms a complex with SNF2H
            in the testis
  JOURNAL   J Mol Biol 415 (5), 793-806 (2012)
   PUBMED   22154806
REFERENCE   8  (bases 1 to 11129)
  AUTHORS   Hung,L. and Kumar,V.
  TITLE     Specific inhibition of gene expression and transactivation
            functions of hepatitis B virus X protein and c-myc by small
            interfering RNAs
  JOURNAL   FEBS Lett 560 (1-3), 210-214 (2004)
   PUBMED   14988024
  REMARK    GeneRIF: Small interfering RNAs have specific inhibition of Hbxap
            expression and transactivation.
REFERENCE   9  (bases 1 to 11129)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149605.7, AC124541.4 and AC151477.1.
            
            On Dec 2, 2009 this sequence version replaced NM_001081267.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777534.844836.1,
                                           SRR10662774.1538066.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-210               AC149605.7         165270-165479
            211-302             AC124541.4         9985-10076          c
            303-395             AC151477.1         56918-57010
            396-601             AC151477.1         59846-60051
            602-756             AC151477.1         73594-73748
            757-2525            AC151477.1         81354-83122
            2526-2732           AC151477.1         85101-85307
            2733-2837           AC151477.1         90310-90414
            2838-2917           AC151477.1         91344-91423
            2918-2972           AC151477.1         95122-95176
            2973-3082           AC151477.1         96697-96806
            3083-3150           AC151477.1         97886-97953
            3151-3358           AC151477.1         99476-99683
            3359-3582           AC151477.1         101160-101383
            3583-3771           AC151477.1         102240-102428
            3772-11129          AC151477.1         105966-113323
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.55 cM"
     gene            1..11129
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="remodeling and spacing factor 1"
                     /db_xref="GeneID:233532"
                     /db_xref="MGI:MGI:2682305"
     exon            1..210
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     CDS             9..4334
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="hepatitis B virus x associated protein"
                     /codon_start=1
                     /product="remodeling and spacing factor 1"
                     /protein_id="NP_001074736.1"
                     /db_xref="CCDS:CCDS40025.1"
                     /db_xref="GeneID:233532"
                     /db_xref="MGI:MGI:2682305"
                     /translation="MAAAAAAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDL
                     PELPFPELERVLQAPPPDIGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQE
                     FNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIG
                     RDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVL
                     LKNSSQQEDSSRESPTLEDEETKKDEETPKQEASKENEKTGADERSVDSGKCSAASAL
                     EEPAVKTEKADEKELAKLPVIVKLEKPLPETEEKKIIREESDSFKENVKPIKVEAKEC
                     RADPKDLKGSLERLGSQEPERADFGSNIKSQDIIEKSTEETEKLKNDQQAKIPLKKRE
                     IKLSDDFDSPVKGPLCKSATPTKEVLKDDIKQEEETCKRVSTISALSHEGKQLVNGEI
                     NDDKVIPNFKTEQMEIQLCDTKEDSASIPAKDGNACMEGNGTECLNSVITSTKTSELE
                     KEAPLGKGIDSSVPDIESLSQKARLEEPGLLNMDMPLEPSEKATDLSLKPTLSATEPC
                     STKVEEKAPRSKSENHTPGIECLERVEKAKKTSIDKDIQKLSPIPEEVVRGALESEKS
                     GPCEVAETPLPLSMAGAKEKQTSEKKDVDCSRGSSESQSLENASPEILKEDSESSREE
                     VAKLDNAQTSGMEDTSETKGSVQKNKFKYKLVSERNSTASENTEITSERKKEGIKLTI
                     RISSRKKKPDCPPQTVDSESKEEKAGKEEEKASVGRTLRRSPRISRPTAKVAEIRVQK
                     ADKRRADGEDGVEGEPASLQTADKKDHLKKAEKDTNSKASKVKPKGKVRWTGSRTRGR
                     WKYSSNDESEGSESDKSSAALEEEEGKEGEEAVLPDDDEPCKKCGLPNHPELILLCDS
                     CDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKE
                     RLVYVGISIENIIPPQEPEFSEEQEEKKKDAKKSKANALERRSTRTRKCISYRFDEFD
                     EAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARR
                     KKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEEPPSNEDSDTDF
                     CSRRLRRHPSRPMRQSRRLRRKTPKKKYSDDDEEEEESEENSRDSESDFSDDFSDDFV
                     ETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSESEESFMSKNS
                     EDDELTKESKRSVRKRGRSTDDYSEADEDDEEEGKPSRKRLHRIETDEESCDNAHGDA
                     DQPARDSQPSALPSEQESSKKPYRIESDEEEDFENKVGSPLDYSLVDLPSTNGQSPGK
                     AIENLMGKATEKSQTPKDNGTASASLAPNGTSGGQEVGAPEEDEDELLRVTDLVDYVC
                     NSEQL"
     exon            211..302
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            303..395
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            396..601
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            602..756
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            757..2525
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2732
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2837
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2917
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..2972
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3082
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            3083..3150
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            3151..3358
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3582
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            3583..3771
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            3772..11129
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4747..4752
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="hexamer: AATATA"
     polyA_site      4765
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="major polyA site"
     regulatory      9519..9524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="hexamer: ATTAAA"
     polyA_site      9542
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="major polyA site"
     regulatory      11103..11108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="hexamer: AATAAA"
     polyA_site      11120
                     /gene="Rsf1"
                     /gene_synonym="4832420A03Rik; C030033M12Rik; Gm164; Hbxap;
                     p325; XAP8"
                     /note="major polyA site"
ORIGIN      
        1 agttcaaaat ggcggcggcg gcggcggcgg cggcggcggc agcggcagcg gcgatggctc
       61 ctccgggctg cccgggttcg tgccccaact tcgccgtagt ctgctccttc ttggaacgct
      121 acgggccgct gctggacctg cccgagttgc cgttcccaga gctggagcgg gtgctgcagg
      181 cgccgccgcc agacatcggc aacggagaag taccaaaaga attggtggag ctgcatttga
      241 agttaatgag gaagattggc aaatccgtta cagcagacag atgggaaaaa tacttgatca
      301 agatctgtca agagtttaac agtacctggg catgggagat ggagaagaaa ggctatcttg
      361 aaatgagtgt tgaatgcaaa ctagcactct taaagtatct ctgtgagtgt cagtttgatg
      421 acaatcttaa attcaagaat ataattaatg aggaggatgc tgatactatg cgtctccagc
      481 caattgggcg agacaaagat ggcctcatgt attggtacca gttggatcaa gaccataatg
      541 tgagaatgta catagaagaa caagatgatc aagatggctc ttcttggaaa tgcattgtca
      601 gaaatcgaaa cgagttggct gagactcttg cactcctgaa agcacaaatt gatcctgtcc
      661 tattgaaaaa ctcgagccaa caggaagatt cttctcggga aagccccacc ctagaggatg
      721 aggagactaa gaaagatgaa gaaacaccta aacaagaagc atcaaaagaa aatgaaaaga
      781 caggagctga tgagcggtca gtggattctg ggaagtgttc tgcagccagc gctctagaag
      841 aaccagctgt gaaaactgag aaagcagatg aaaaggagct tgcgaaactg ccagtcattg
      901 taaagctaga aaagcctttg ccagaaactg aggaaaaaaa gattatcaga gaggaaagtg
      961 attctttcaa ggaaaatgtc aaacccatta aagtagaagc aaaggaatgc agagcggacc
     1021 caaaagatct caaaggcagc ttggagcggc tgggctcgca ggagcctgag cgagccgact
     1081 ttggtagcaa catcaaatct caagacatca tagagaaatc tactgaggaa acagagaagc
     1141 ttaaaaatga tcagcaagcc aagataccac tgaagaaacg tgaaattaag ctgagtgatg
     1201 attttgacag tccagtcaaa ggacctttgt gtaagtcagc tactccaact aaagaagttt
     1261 tgaaagatga cataaaacaa gaggaagaga cttgtaaacg ggtctctacc atcagtgctt
     1321 tgagtcatga agggaaacaa cttgtgaatg gagagattaa tgatgacaag gtaattccaa
     1381 attttaagac agaacaaatg gaaatacagc tttgtgatac aaaagaagat agtgctagca
     1441 tccctgctaa ggatggaaat gcctgcatgg agggaaatgg tacggagtgc ttaaattctg
     1501 tcataaccag cacaaagaca agtgagcttg agaaagaggc ccctctaggg aagggtatag
     1561 acagctctgt acctgacata gagagcctca gtcagaaagc aaggttagag gagcctgggc
     1621 ttctgaacat ggacatgcct ctcgaacctt ctgagaaagc cacggatctc tctttaaaac
     1681 ccactttgtc tgccacagaa ccctgcagta cgaaagttga agagaaggcc cccagaagta
     1741 agagtgagaa ccacacccca ggcatagaat gcctggaaag ggtggagaaa gccaagaaga
     1801 cctccattga taaggacatc caaaaattga gtccaatacc agaagaagtt gtaaggggtg
     1861 ctctagagtc agaaaagtca ggtccttgtg aagtggctga aactcctctc ccactctcga
     1921 tggctggggc caaggagaaa cagacctcag aaaaaaagga tgtagactgt agtagaggtt
     1981 cgagtgaaag tcagtccctt gaaaatgcaa gcccagaaat tctaaaagag gattctgagt
     2041 cctcgaggga ggaagtggcc aaactggaca atgcccagac ctctggcatg gaggatactt
     2101 ctgaaacaaa gggctctgtg cagaaaaaca aatttaaata taagttagtt tctgaaagaa
     2161 acagtactgc ttcagaaaac actgagatca cctctgaaag gaagaaggaa ggcatcaagt
     2221 tgacaattag gatatcaagt cggaagaaga agccagattg ccccccacaa actgtagact
     2281 cagaaagcaa ggaagagaag gcgggcaagg aagaagagaa agcaagtgtg ggtcgaactt
     2341 taagaaggtc tccaagaata tctagaccca cagcaaaagt cgctgagatc agagtccaga
     2401 aagccgataa gagaagagct gacggagaag atggagtgga aggggagccg gcatctttgc
     2461 aaacagcaga caagaaggat cacttgaaga aagcagagaa ggatacaaat tctaaagcaa
     2521 gcaaggtaaa acccaaaggc aaagttcgat ggactggctc tcggacacga ggcaggtgga
     2581 aatactccag caatgacgaa agtgaaggat ccgagagtga caaatcctct gcagccttgg
     2641 aagaggagga aggaaaggaa ggtgaagaag cggtccttcc agatgacgat gaaccttgca
     2701 aaaagtgtgg ccttccgaac catccagaac taattcttct gtgtgattct tgcgacagtg
     2761 gataccatac tgcctgcctc cgccctccgc tgatgattat ccctgatgga gaatggttct
     2821 gccctccttg ccaacataaa ctactgtgtg aaaagttaga agaacagctg caagatttgg
     2881 acgtcgcctt aaagaagaaa gagcgtgcag agcgaaggaa agaacgtttg gtgtatgttg
     2941 gtatcagtat tgaaaacatc attcctccac aagagcctga gttttctgaa gagcaagaag
     3001 aaaagaaaaa agatgcaaaa aaatccaaag caaatgcact tgaaaggaga tcaacaagaa
     3061 caaggaaatg tattagctac agatttgatg aatttgatga agcaattgat gaagctatag
     3121 aagatgatat taaagaggct gatggaggag gagttggccg ggggaaagat atctccacca
     3181 tcacaggtca ccgtgggaaa gacatttcta ctattttgga tgaagaaaga aaggaaaata
     3241 aacggcccca gagggcagct gctgctcgca ggaagaaacg ccggcgactt aatgatcttg
     3301 acagtgatag caacctagat gaagaagaga gtgaggatga attcaagatc agtgacggat
     3361 ctcaggatga gtttgttgtg tctgatgaaa atccagatga aagtgaagaa gagccaccat
     3421 ccaatgagga cagcgacact gacttctgca gccgaagact gaggcggcat ccttctcggc
     3481 caatgaggca aagcaggcgt ttgcggagaa agaccccaaa gaagaagtac tccgatgacg
     3541 atgaggagga ggaagaatcc gaggagaaca gcagagactc tgaaagtgac tttagcgatg
     3601 attttagtga tgattttgta gaaacccggc gaaggcgatc aagaaggaac cagaaaagac
     3661 aaattaacta taaagaagat tcagaaagtg atggttccca gaaaagtcta cgacgtggta
     3721 aagaaatcag gcgagttcac aagcgcagac tttccagctc agagagtgaa gagagcttta
     3781 tgtccaagaa ctctgaagat gatgagctaa caaaagaatc gaagcgatct gttcgaaaac
     3841 ggggccgaag cacagatgac tattcagaag cagatgagga tgatgaagag gaaggcaaac
     3901 catcccgcaa gcggctacac cggattgaga cggacgagga gagctgtgac aatgctcatg
     3961 gggacgcaga ccagcctgcc cgtgacagcc agcccagtgc cctgccctca gagcaggaga
     4021 gcagcaagaa gccctaccgg atagagagtg atgaggaaga agactttgaa aacaaagtag
     4081 ggagcccatt ggactacagc ctagtagact tgccttccac caatggacag agccctggca
     4141 aggccattga gaacttgatg ggcaaagcaa ctgaaaagtc ccaaaccccc aaggacaatg
     4201 gcacagccag tgcaagtcta gcccccaatg ggacaagtgg tggacaggag gtgggggcac
     4261 cagaggaaga tgaagatgag cttttgcgag tgactgatct tgttgattac gtctgtaaca
     4321 gtgaacagtt ataagacttc tttccattct tgtgctaatt tattccacgg tagctctcac
     4381 accagcgggc cagttagtaa agctgtttaa tttttcctag aaaactacag aacgactttt
     4441 tagaagaaaa atttcaacaa atcttgaagt ctttctggga agtgaccagt tttgaacttt
     4501 gaagattaat aattgctgta aattcctttt gattttcttt ttccaagttc atggtccttg
     4561 gtaatttcat tcatgggaaa aaatcttatt ataataacaa caaagatttg tatatttttg
     4621 actttatatt tcctgagctc tcctgacttt gtgaaaaggg tggatatgaa tgcattccaa
     4681 atctgtgagg gcccaaaaca gaatttaggg tgggagaaag cacctgtgct ttagcttttc
     4741 atattaaata tatattatat ttaaacattc atggcataga tgatgattaa cgaactgttt
     4801 aaaagttcat gtctgtactg ttgcagtttg agtattgtag ataacatcac acaaacatca
     4861 cttagtaata gcttttgtga catcaattgg tttacttttg gagttaccac actttaaata
     4921 aaaaaagaga ccctggaagc accatcataa aaacatttta acctaagttt ctgttatagc
     4981 agagtttctt gttataaaaa aaaaatcatc tggaaagtat ttgtatagta aaatgtataa
     5041 tgaagctttg acaaccagat tgtgctaaca gaatcttttc aatagcttta aagcttttgt
     5101 aataaattag atggcctata catgctgggc caggtgggca gttagtgaaa tgagtggtct
     5161 ttccctaaaa tctaggtaca ctataaactt gacagtagtg ttaactcttc aggccacatg
     5221 gtgttcacca tctgggatgc cattttgaat ataatccatc taatataaca tggagatgct
     5281 aagctgtgta ttttagcaca tttgaagact gagaagggat tttcaggtga attttaactg
     5341 gtttattctt gcccttacta tctacttcaa attgaagtct acaaacaaag cagttccttt
     5401 gggaggtttt tagtttttag tttaagcatg tttatggtgt gtgatttgta tgatgtgtgt
     5461 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgaattttc
     5521 tatcggcctg gatatattag cagggtttga atgtagtttt ggcctttggc cattatatac
     5581 ttctattaaa atccattaat agtcacacaa ccaacagaat taaatgagaa ccgctgatat
     5641 atttaataag tataatttcc attttaaaca tctcaacact ttaaggataa gccctttgct
     5701 tcaagaatag gcggttttta aatcaatata acatagctga cactaaaata tgaagttata
     5761 ccttagtttc tgttacagtt gtgaaagttc aggcacagcc ctagccctgt acagctgtag
     5821 cacttgtgac tcgccttgcc tggcacatct gaagccttca gcattgcttc agtgtgtgct
     5881 tttgccctga tattcgggtt ttctgtccca gtgttgtctg ttgccggcaa tagacatgct
     5941 gtatctgctg ctggctcaag gaatttcact tttctttaag attaagcatt atatgtagtc
     6001 agtgtacagt aaaccacttt ttattattat tattattgaa agctggcatt agacttgtgt
     6061 cataaaagcc tctggatact ttatgctcct actttttgtt ccttgacaga tacactctta
     6121 aatcttctct cctggccttt aaattttagt gctgattttt tttttttcag ttatttgatt
     6181 ttggtttcac tttagttcca tagaacttaa gtgttaatgt ttgctttgat tgtagcagtg
     6241 aacttacatt tgtgtaacaa taaaacaatg aaagcagcca ctgcagagcc agctgtggct
     6301 tcattttgac atagtgttgt gctagaagga cagggcgggc ttacattcac aggcagtgaa
     6361 ggactcaaag gcaccactta cttgcataac ttcctttcta gctgtcgtgt tacttgaaaa
     6421 tcagctaagg aagtttttca ctgtctagaa caggcaggat tcctactact gtgtggtaag
     6481 tggttcttcc tacactctca gaaggcttca gtcctgcaat caggtaaagc cactgtggtg
     6541 tagaaaatgt cctatgtgcc tgtctctacc tctgcacaca ccagctcttg caaccaccta
     6601 tcaattctat tcatcattta cacagatgat tagaagtagt aaatgggtag ctaactcaac
     6661 agcgtgcaga atgtgacgta acttcttatt cttctgctat ggaaacccac agtaattttt
     6721 tatagcaaag actgagtatt ccacttagca aatgccccac tgacttgggt ttcaaaaacc
     6781 acttatgcta cctgatctta ctacacttga gtgtcacctt cataaagagt gtctcccttc
     6841 taactttaca ttctcattag accaactggc tataaagagt aatccaggca gctaatatat
     6901 agcaattctt agtatccagc tacttctgct tggctttttt gttgtctctg ttcatgctgg
     6961 agatcgaatc cagggcctca tgcatactgg gtaagtgttt taacgctggg ctacaactct
     7021 tgccctgctt agtgttcaga ttcagtccct cagagtctgt tatctctcag aaacccttac
     7081 ttggaagatg ttctcaatga ggtttctgtt gctgctgtca aaataaatgt tctgtatcaa
     7141 aaattctgat aaaatcatta agaactggag aatggaaagg aaaacaggca tgtagactat
     7201 cttcaatctt gatagattca gatttataat agatttttag agttttcccc tttaatattt
     7261 tcagtgaccc cccctcctac cttttgacat acaaatgttg aacttgtatc aaagggctcc
     7321 cagtgtcaga ctcctagcct acagcatatg cattaagggg ggatacagtg taatattcca
     7381 agtgtttagt ttgcgatttc agtaaggtgt gagaggtagc cagattttta cagttccaga
     7441 acttagaatt gaaacagtgt atgaaagtga cactgatggg tttgtccacg ctgtgccgat
     7501 acagtgcttg ttgtagagtg taactgcaga gtcgtgtgtt tgcaacaaca cacatgccac
     7561 actgaggtgg tttccttcac ttcttccact tggtcaaggg tacagagtga gaggataaaa
     7621 agttgcatta agaataaatt catgtttact ggtaactcat tttttaaaaa gtgatctgcc
     7681 aaagaaccca gaacttcaga tactagtgaa aaattgttgt ggggaaaaga tttaaaaaag
     7741 atgttattac ttttcacttt ctagttgata ggacttatcc cacataataa acattgaagc
     7801 caagtgcata tattaagaaa aatgtggaca cttacttgct tgtttaaata gggagttata
     7861 attatggaga atgtgtcagt aattgtaatg tgggatgagt ggagttccct ttgtgacaag
     7921 aataccgttt agcctcgttg gtaggcatga ggtgttagcc ctcactagtt cttctaccag
     7981 aagacagaaa acaccgaaca gatctgtaac tctcagaaaa tgctgtgact tcactgccag
     8041 ctgtcacagt ggagcgggtc tttagttcag ggtggtatct gagctttaga acagatgctc
     8101 aacgatattt cttacgagga aatgtgtgca tacacacatt ctgggtaacc acaaggatgc
     8161 tgaattcatc atgaactgag ttactaaatg tataaggcaa atgattgtga caaaattatg
     8221 aatatgattc aacgataggt ttaaagaaaa aaacagtttt tcttgatggt ttgacattct
     8281 caagacgttt ctggttctct tagcagagaa tgccaagacc acatgtatgc tgtaatctac
     8341 atagtccttg tttgttaata tgatccatta gtgaactctc agaacaaaat tcattcctat
     8401 tttatccgct attcccagag aaaccctgga agtggcaagc aagctagctg ctgttttatt
     8461 tcctcactgt tcttatcctg atcctgaaag gaaaacaaaa caaaacaaac aaaaaaaaaa
     8521 aaacctttgt tctcatccag ttttagactg gattatcgtt atgcttccat ttaatttcca
     8581 aatattttgc tccaacttaa atgaggtaac tttttctttt gagtgccaag gtttgctaga
     8641 taactggcta cacaacactg aggacaagct attcccatgt aaacagtcat aagcgaaaat
     8701 gaggtagcgt tggtggcggg ccacgttaga ctgtacactc agcccttgtt gctcatacag
     8761 accctctcag aagctgatta attagaacag aggtctaatt ccccactcct ggtcatgcac
     8821 acagctccct gccctatctc acatttatac cactttgctc taggagaccg ttctggcaca
     8881 ctcaccatag gggtttcagt tcgtggaggt gttctgatct taataatgtt ttagatacat
     8941 ctatcccata cacaagctat gtgatagaat gtaacaaatt accagtgaga agggatttat
     9001 taagctctag atattttgaa tgagagaaaa actaaggaac tatatttggg ggagggttgt
     9061 tttgtttttt tgttttttgc caaactggca caagctagaa catgtgcaaa aggctgaata
     9121 agctccctgt tctttttgcc actggatgat ggaagaatga gcttgtctag gtatatattt
     9181 tcttgacttt caccagtttc agaaggagtc taatttaaac atttggggtt tgtttgatct
     9241 gtatttattg catcctggcc ataggccggg ggcctgcatg ttatgtttat gaacagctag
     9301 aacacagcca tgtggaactt aatggctcaa gatcttctaa ataatttaac ttattttgta
     9361 tgttgcaaaa aagaaaaaaa aagtttattt tgcttgagag tcacattttt ttataactgt
     9421 acagctttta agggatggga ggtggggaaa taccctaaaa taggatgtag atttttacag
     9481 ttgtgtttat gctagaacat ctatgggagc attatgtaat taaacctcaa aattgtttaa
     9541 aaccttgtgc catggtcttt ttcattatac ttccctttag caagagttgg tgcatgtcaa
     9601 tcgtgcactg ctgtgtggac agaccctgag attagattaa tacctatttc caaagctttc
     9661 atccattgca tgaattacct ccctacacac acgcacacat gcacacacag aggtacagtt
     9721 cctagtcgtg taaatgcatc ttcaggatca gtataactga acacgcagac cctggcaacc
     9781 agcagcatta ctcttcccgt gcatgacttt gtggtgttgt ctaggctgtt actgatgttg
     9841 ctgtgttttg ttctcttatc aaagtggctt cttggtcata gtgtctggat agtaccttgc
     9901 ccaaaacccc cattgggttt cacgtgcact ttaagaaggg atgtcctatg ttgagatgga
     9961 gatgggaatg gagatgggaa tggggcaggg ccagtttctt atgtgtaaga gaaaaactag
    10021 aattgttgac tgttctgtag acttacctaa ctctacacaa ataacttgct gaaacttggc
    10081 aggaaaggaa atcacattaa cttttctatc cctccttatt tggtaaataa ttttgtaatc
    10141 tattagaagt gctgctgctg ccagtcttcc ctggcacatt ttcccatgct atagccacac
    10201 ctttttaaat gtcagtactc cccccaaccc ccactccgct atccggtaat ttcaccattc
    10261 tggtttacta cctggatagc acaactgtat tcttataagt aattaggttt atctaaatgt
    10321 gacaatttct aagtaacctt catctgctgg ctactaattt ggaacataat tttctttgct
    10381 atttctatcc atttagaagc ttaggggccg tgagtttcaa tgtgaagagt ataaattcca
    10441 gctaagagtt tgtaaattgt cttatcttag aatctcactt caattttaaa gtagtaacta
    10501 agttccagga agcacctaat cagaacatgt cttggatttt aagatgtgtt tatttgggat
    10561 actttatttt ccctatgcat aaaagaaaaa tattgctgga atagttctcc aactactgtt
    10621 tttagatgag agctgaagca gaagctccaa gactagcaat caaagtaact cttgaggtaa
    10681 ttaggatcat gctgttagtc aaagagaact gggagtgcgc tgtcacataa taatgtactg
    10741 cacatgccaa tgaggtgcac aaagatctgc aatggtgacg ctgagaacac aagtgaagtg
    10801 aagtttgtgt atggagagta tggcaggtcc tgagagcttc aagcttcagg agtgttagaa
    10861 ctatagttca tagttgttta aatataatat ttcttgtgac aaccaaaata tgttgtggct
    10921 ggtgccaata ttttcgttaa tgaaatgttg aatgtcgcat tacagtctga tcagaattct
    10981 cagtgtaata agccaggcct aagccatttt atagctattg gcctaactgt gtaacttttt
    11041 ttttcaaaat gttttattat aatacttgtc atttctttca cttaatatat gttaattgtc
    11101 ataataaaaa gtttaaataa gttgatgta
//
