LOCUS       NM_025897               2856 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ribosomal RNA processing 8 (Rrp8), transcript variant
            2, mRNA.
ACCESSION   NM_025897
VERSION     NM_025897.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Murakami,S., Suzuki,T., Yokoyama,W., Yagi,S., Matsumura,K.,
            Nakajima,Y., Harigae,H., Fukamizu,A. and Motohashi,H.
  TITLE     Nucleomethylin deficiency impairs embryonic erythropoiesis
  JOURNAL   J Biochem 163 (5), 413-423 (2018)
   PUBMED   29244083
  REMARK    GeneRIF: The suppression of p53 did not rescue the lethality or
            impaired erythropoiesis of NML-/- embryos, suggesting that
            p53-independent mechanisms underlie the NML-deficient phenotypes.
REFERENCE   3  (bases 1 to 2856)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2856)
  AUTHORS   Oie,S., Matsuzaki,K., Yokoyama,W., Tokunaga,S., Waku,T., Han,S.I.,
            Iwasaki,N., Mikogai,A., Yasuzawa-Tanaka,K., Kishimoto,H.,
            Hiyoshi,H., Nakajima,Y., Araki,T., Kimura,K., Yanagisawa,J. and
            Murayama,A.
  TITLE     Hepatic rRNA transcription regulates high-fat-diet-induced obesity
  JOURNAL   Cell Rep 7 (3), 807-820 (2014)
   PUBMED   24746822
REFERENCE   5  (bases 1 to 2856)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AV460242.1, AK005138.1 and AC121823.3.
            
            On Feb 3, 2011 this sequence version replaced NM_025897.1.
            
            Transcript Variant: This variant (2) uses an alternate exon in the
            5' UTR and 5' coding region, compared to variant 1. It encodes
            isoform 2, which is shorter and has a distinct N-terminus, compared
            to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165828.1, BC022923.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                AV460242.1         1-84
            85-2377             AK005138.1         1-2293
            2378-2856           AC121823.3         134360-134838
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 55.97 cM"
     gene            1..2856
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /note="ribosomal RNA processing 8"
                     /db_xref="GeneID:101867"
                     /db_xref="MGI:MGI:1914251"
     exon            1..287
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..1562
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ribosomal RNA-processing protein 8; ribosomal RNA
                     processing 8, methyltransferase, homolog; cerebral protein
                     1 homolog; nucleomethylin protein"
                     /codon_start=1
                     /product="ribosomal RNA-processing protein 8 isoform 2"
                     /protein_id="NP_080173.1"
                     /db_xref="CCDS:CCDS21658.1"
                     /db_xref="GeneID:101867"
                     /db_xref="MGI:MGI:1914251"
                     /translation="MFEEPEWVEAAPAIVGLGAATAQVRPATAPPVKGRKRRHLLATL
                     RALEAASLSQQTPSLPGSDSEEEEEVGRKKRHLQRPSLASVSKEVGKKRKGKCQKQAP
                     SISDSEGKEIRRKCHRQAPPLGGVSAGEEKGKRKCQEYSSLHLTQPLDSVDQTVHNSR
                     TSTATIDPSKPSPESMSPNSSHTLSRKQWRNRQKNKRRHKNKFRPLQTPEQAPPKASI
                     EETEVPPVPKSDSQESRAGALRARMTQRLDGARFRYLNEQLYSGPSSAARRLFQEDPE
                     AFLLYHRGFQRQVKKWPLHPVDRIAKDLRQKPASLVVADFGCGDCRLASSVRNPVHCF
                     DLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKTGGLLKVA
                     EVSSRFEDIRTFLGAVTKLGFKIIYKDLTNSHFFLFDFEKTGPPRVGPKAQLSGLKLQ
                     PCLYKRR"
     exon            288..654
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     exon            655..1108
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1238
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1345
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1442
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..2856
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2361..2366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /note="hexamer: AATAAA"
     polyA_site      2379
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /note="major polyA site"
     regulatory      2550..2555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
                     /note="hexamer: AATACA"
     polyA_site      2579
                     /gene="Rrp8"
                     /gene_synonym="1500003O22Rik; 2900001K19Rik; NML"
ORIGIN      
        1 tgcctctcta gctcgccagc ggctacgctc tatggtgctc tgctccgcga aggccgctct
       61 agctaccaca gaatttcact cggtggcttc cggcccggtt tggtccgact ccagccctag
      121 gattccctga cgcgcgctgc acgtgggcca gagcgtaggc tcctccggcc ctgcgggcta
      181 cgggtggtat gttcgaagag cccgaatggg tggaggcggc cccagccatc gtgggcctag
      241 gggccgcgac agcacaggtt aggccggcga ctgccccgcc agtcaaggga cgcaagcgcc
      301 gccatctctt ggccacatta cgggctctgg aagcagcatc tctctcccaa caaaccccca
      361 gcctacctgg cagtgactct gaggaggagg aggaggtagg aaggaagaag agacacctcc
      421 aaaggccctc acttgccagc gtctcaaagg aagtagggaa gaaaagaaaa gggaaatgtc
      481 agaaacaggc gccatccatc agtgactctg aggggaaaga aataagaaga aaatgccaca
      541 gacaagctcc tcctcttggt ggggtctctg ctggagaaga aaaaggaaag agaaaatgcc
      601 aggaatattc ctctttacac ctaacccagc ccctggacag tgttgaccaa acagttcaca
      661 attccaggac gagtactgct acaattgacc catccaagcc aagccctgag tctatgtcac
      721 ctaactcctc acacaccctg agccgcaagc agtggcggaa ccggcagaaa aataagcgga
      781 gacacaagaa caaatttcgg ccactccaga caccagagca ggctcctccc aaggcttcca
      841 tagaggagac tgaggtgcct cctgtcccaa agtcagatag tcaagagtct agagctggag
      901 ccctgcgagc acgcatgaca caacgcctgg atggggcccg atttcgctac cttaatgaac
      961 agttgtactc agggcccagc agtgctgccc gacgcctatt ccaggaagac cctgaggctt
     1021 ttctccttta tcaccgtggc ttccagagac aagtaaagaa gtggccactg cacccagtgg
     1081 accgtattgc caaagatctc cgccagaagc ctgcatcctt agtggtagct gactttggct
     1141 gtggagattg ccgcctagct tcaagtgtcc ggaaccctgt gcactgtttt gatttggctt
     1201 ctctggaccc cagggtcaca gtatgtgaca tggcccaggt gcctctggag gatgaatctg
     1261 tagatgtggc tgtgttttgc ctttcactga tgggaactaa catcagggac ttccttgagg
     1321 aggcaaatcg agtgctaaaa acagggggtc ttctcaaagt agctgaagtc agcagccgct
     1381 ttgaggatat tcggaccttt ttgggggctg tgaccaaact cggctttaag attatctaca
     1441 aggacctgac caacagccac ttcttcttgt ttgactttga aaagaccgga cctcctcgag
     1501 taggacccaa agcccaactc tcaggcctta aacttcagcc ctgtctctac aagcgcaggt
     1561 gaccgatgga cacccattgg aaggggagag ctcagactcc agtctcagaa ctctgaagac
     1621 tgtatctacc caggctatga gccaggatct ggtaccaccc tccttctgta ccaagaacac
     1681 tgtggtaaag cctccagctc agctctactc taatggagcc ttcttggata cagaaagcat
     1741 aaaattactt gggcaggctc aagaatgagg tgtttattgt gagggcatgg atgtgtctaa
     1801 taattgtggg tcacttcagt attgtggaaa ctagtcggct gagaactgat ggatctctgg
     1861 gtttgctttc tcttcatctc tcagaaggca cgtgggctct ccgctgtgtt gtcttctgac
     1921 caatattgtc cacctctgct catccatagt ttctgctcct tcagcttcaa gactacagac
     1981 aggcagtttt tgccttatgg caggtcccaa tgctagtttg ccacttgggg ctgggagtta
     2041 tttaatttct ttgtttcttg gagctgggaa tcatctaatt tttttctgtg ttgctattgt
     2101 tgttgttgtt gttgttgttt tgagacagta tctcacacta gtccaagctg acctcaaaca
     2161 aaacaaatgg caatcctcct gcctcagcct cctgattatt gggaacacag gcatgggcca
     2221 ccatatttag ctgaagaaat catttacttt ctgaatattc tatttctcat ttgaaaaatg
     2281 taaccttctt gtcttacagt atgttaaatg ggttcactga ggaaagctac agctagagct
     2341 gggactagta agaggtgctg aataaaccag ctatggctat gtgtgtcgtt gatctcactg
     2401 tgtctgatca cccctactgc cttaacggtt gagcaataag taatgtagat gtgaccgaga
     2461 aagactgggg gagtttgata taggcatgta aacgggctta tttgctcttt agagacttaa
     2521 cgtgtttgaa gctttccttg gaagggggta atacagaagc tggaatctgt tcaacagcag
     2581 aaactgattg ggctgcttat tggcacagtc tgtgtgtcct aggaaaacta caggtactga
     2641 atatacaagt tcttggcgta gtgctgaggg aaggtgagct aggttgacac actgagctca
     2701 ttcaggaggc gtctgccttg aaaccaggca tataggcttg tttacgactt cctaagaata
     2761 agacctagga gtttcctcta ggagcaaata ggtctcggga ttttgaacca gttagatgcc
     2821 tccaggccta aaggaataaa gattttgaaa aaggcc
//
